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The map label for this gene is OppA2 [H]

Identifier: 20806734

GI number: 20806734

Start: 213609

End: 215255

Strand: Direct

Name: OppA2 [H]

Synonym: TTE0208

Alternate gene names: 20806734

Gene position: 213609-215255 (Clockwise)

Preceding gene: 20806733

Following gene: 20806735

Centisome position: 7.94

GC content: 36.73

Gene sequence:

>1647_bases
ATGAGGGGGAAAAAGAGCATAATTTCACTATTTGTTGCAGTGTTATTTATCTTATCTTTAAGTTTTTCAGGATGTTCTAA
ATCTTCTAATCCTATATCTTCCCAAAAAGTACTAGCAGATGAGCAGGTTTTAAATATTAATTTAGGGGAAGAACCGCCTT
CTCTGGATCCGCAGTTGGCTACTGATTTAGTATCATTTGAAATGTTAAATGCTACATTAGAAGGGCTAGTGAGGCTAAAT
AAATACGGGAAAATAGAAAAAGGTTCTGGACTAGCTTTAGATTGGGAAGTTTCTCCAGATGGGTTGAGGTATGTTTTTCA
CTTGAGAGATGCAAAGTGGAGCGATGGAACCCCTATAACTGCTCATGACTTTGAATATGCTTGGAAGAGGGCTCTTGACC
CGAAAACAGGGTCAAACTATGCGTACATGCTCTATTATTTAAAAAATGGGGAAGCTTTTAATAAAGGGGAAGCTAAGGCA
GAAGATGTAGGTGTAAGGGCTCTTAATGACAAAACGCTAGAAGTAATACTCGAAAGACCAGCTCCGCAATTTTTGGGTTT
GACAGGATGGGGTACTTATTTGCCTGCTCAAAAAGCGGCTATTGAAAAATTTGGAGATAAATATGGAACCTCTCCTGATA
CGATGGTCTATTCCGGTCCTTATATGATAAAAGAATGGAATCACGAACAGAATATAGTACTTGTAAAAAATCCCTACTAT
TGGGATAAGGATAGTGTGAAACTTCAACAGATAAATATGGACATGGTAAAGGAAGAAAATACTATTATTCAAAACTGGGA
AACAGGGCGTTATGATTCTATTAGTGTACCTGCCCAATATATTGCAAAATATAAAAATGATCCGAATTTTCACCAAATGC
CAATAGGTGTAACTTACTATGTCATGTTCAACAACAAATCCAAGATATTTTCAAATGTAAATATAAGAAAAGCTTTCAGT
TATGCGATTGATAGGCAGTCTTTTGTAGATTATGTTTTCGCTGGCGGAGGGATACCTGCTCTCGCTTTTGTGCCACCAGG
AATACCTGGAGATAAAGAAGAATTTAGAAAAGAAGGCGGGGATTACTTTAAAGATAATGACGTAGCAAAAGCGAAAGAAT
TGCTTGCACAAGGCATGAGAGAACTAGGAATTGATAAGCTCCCCAAGATTAAATTTGTAGCTGGAGACAGTGATGCAGCG
AGGAAACATTCACAAGTATTACAAGAGTTTTGGAGTAAAAATTTAGGTGTAACTGTTGATATTTACAACGTAGCTTTCAA
AGTAAGACTTGATATGATGAATAAAGGAGATTACGATATAGTGTATACTAATTGGGTAGCTGACTACAACGACCCAATGA
CATTTTTAGACCTTTGGGTTACAGATGGAGGTAATAACAGAGCTTTTTATAGCAACCCTCAATATGATCAACTCATAAAA
AAGGCTAATTCTACAATTGATAATTCCGTTGGAATGCAGGCTATGCATGAGGCAGAGAATATATTGATGGAGGATATGCC
TATTGCTCCTTTACTTTTTAGGATTACCTCGTATTTAGAAAGACCCTATGTAAAAGACTGGATAAGATTTCCTGTGGGGA
TAGACGATGAGTGGAAATGGACGTATATAGAAGGTAAAAATAAATAG

Upstream 100 bases:

>100_bases
AATGATATAATTATTTATAACCTTGAATAAAAATTTATGACATTCAAGAAGTGACGAGAGCCAGAGTTTTTACAATTTAT
AAGGGGGAGTGGAGTGACTA

Downstream 100 bases:

>100_bases
TGCTTTAAAAAGGGTCAAGCTTATAAGCTTGGCTCTTTTTAAGATGTTTACAGATATTCCCGCAAGTGTTAGATAAATTT
CTTAGATAAATTTCTGTATT

Product: ABC-type dipeptide/oligopeptide/nickel transport systems, periplasmic components

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MRGKKSIISLFVAVLFILSLSFSGCSKSSNPISSQKVLADEQVLNINLGEEPPSLDPQLATDLVSFEMLNATLEGLVRLN
KYGKIEKGSGLALDWEVSPDGLRYVFHLRDAKWSDGTPITAHDFEYAWKRALDPKTGSNYAYMLYYLKNGEAFNKGEAKA
EDVGVRALNDKTLEVILERPAPQFLGLTGWGTYLPAQKAAIEKFGDKYGTSPDTMVYSGPYMIKEWNHEQNIVLVKNPYY
WDKDSVKLQQINMDMVKEENTIIQNWETGRYDSISVPAQYIAKYKNDPNFHQMPIGVTYYVMFNNKSKIFSNVNIRKAFS
YAIDRQSFVDYVFAGGGIPALAFVPPGIPGDKEEFRKEGGDYFKDNDVAKAKELLAQGMRELGIDKLPKIKFVAGDSDAA
RKHSQVLQEFWSKNLGVTVDIYNVAFKVRLDMMNKGDYDIVYTNWVADYNDPMTFLDLWVTDGGNNRAFYSNPQYDQLIK
KANSTIDNSVGMQAMHEAENILMEDMPIAPLLFRITSYLERPYVKDWIRFPVGIDDEWKWTYIEGKNK

Sequences:

>Translated_548_residues
MRGKKSIISLFVAVLFILSLSFSGCSKSSNPISSQKVLADEQVLNINLGEEPPSLDPQLATDLVSFEMLNATLEGLVRLN
KYGKIEKGSGLALDWEVSPDGLRYVFHLRDAKWSDGTPITAHDFEYAWKRALDPKTGSNYAYMLYYLKNGEAFNKGEAKA
EDVGVRALNDKTLEVILERPAPQFLGLTGWGTYLPAQKAAIEKFGDKYGTSPDTMVYSGPYMIKEWNHEQNIVLVKNPYY
WDKDSVKLQQINMDMVKEENTIIQNWETGRYDSISVPAQYIAKYKNDPNFHQMPIGVTYYVMFNNKSKIFSNVNIRKAFS
YAIDRQSFVDYVFAGGGIPALAFVPPGIPGDKEEFRKEGGDYFKDNDVAKAKELLAQGMRELGIDKLPKIKFVAGDSDAA
RKHSQVLQEFWSKNLGVTVDIYNVAFKVRLDMMNKGDYDIVYTNWVADYNDPMTFLDLWVTDGGNNRAFYSNPQYDQLIK
KANSTIDNSVGMQAMHEAENILMEDMPIAPLLFRITSYLERPYVKDWIRFPVGIDDEWKWTYIEGKNK
>Mature_548_residues
MRGKKSIISLFVAVLFILSLSFSGCSKSSNPISSQKVLADEQVLNINLGEEPPSLDPQLATDLVSFEMLNATLEGLVRLN
KYGKIEKGSGLALDWEVSPDGLRYVFHLRDAKWSDGTPITAHDFEYAWKRALDPKTGSNYAYMLYYLKNGEAFNKGEAKA
EDVGVRALNDKTLEVILERPAPQFLGLTGWGTYLPAQKAAIEKFGDKYGTSPDTMVYSGPYMIKEWNHEQNIVLVKNPYY
WDKDSVKLQQINMDMVKEENTIIQNWETGRYDSISVPAQYIAKYKNDPNFHQMPIGVTYYVMFNNKSKIFSNVNIRKAFS
YAIDRQSFVDYVFAGGGIPALAFVPPGIPGDKEEFRKEGGDYFKDNDVAKAKELLAQGMRELGIDKLPKIKFVAGDSDAA
RKHSQVLQEFWSKNLGVTVDIYNVAFKVRLDMMNKGDYDIVYTNWVADYNDPMTFLDLWVTDGGNNRAFYSNPQYDQLIK
KANSTIDNSVGMQAMHEAENILMEDMPIAPLLFRITSYLERPYVKDWIRFPVGIDDEWKWTYIEGKNK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=515, Percent_Identity=32.2330097087379, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87081878, Length=534, Percent_Identity=32.3970037453184, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789397, Length=521, Percent_Identity=31.8618042226488, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1787762, Length=517, Percent_Identity=25.7253384912959, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1787052, Length=513, Percent_Identity=26.3157894736842, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1789966, Length=548, Percent_Identity=23.7226277372263, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1787551, Length=469, Percent_Identity=23.0277185501066, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1789887, Length=277, Percent_Identity=24.9097472924188, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62352; Mature: 62352

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGKKSIISLFVAVLFILSLSFSGCSKSSNPISSQKVLADEQVLNINLGEEPPSLDPQLA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCHHHH
TDLVSFEMLNATLEGLVRLNKYGKIEKGSGLALDWEVSPDGLRYVFHLRDAKWSDGTPIT
HHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEE
AHDFEYAWKRALDPKTGSNYAYMLYYLKNGEAFNKGEAKAEDVGVRALNDKTLEVILERP
ECCHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHCCEEEECCCEEEHEECCC
APQFLGLTGWGTYLPAQKAAIEKFGDKYGTSPDTMVYSGPYMIKEWNHEQNIVLVKNPYY
CCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCEEEEECCCCCCEEEEECCCE
WDKDSVKLQQINMDMVKEENTIIQNWETGRYDSISVPAQYIAKYKNDPNFHQMPIGVTYY
ECCCCCEEEECCHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHCCCCCCEEECCCEEEE
VMFNNKSKIFSNVNIRKAFSYAIDRQSFVDYVFAGGGIPALAFVPPGIPGDKEEFRKEGG
EEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCHHHHHHCCC
DYFKDNDVAKAKELLAQGMRELGIDKLPKIKFVAGDSDAARKHSQVLQEFWSKNLGVTVD
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEE
IYNVAFKVRLDMMNKGDYDIVYTNWVADYNDPMTFLDLWVTDGGNNRAFYSNPQYDQLIK
EEEEEEEEEEEECCCCCEEEEEEEEECCCCCCEEEEEEEEECCCCCCEECCCCCHHHHHH
KANSTIDNSVGMQAMHEAENILMEDMPIAPLLFRITSYLERPYVKDWIRFPVGIDDEWKW
HHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEE
TYIEGKNK
EEEECCCC
>Mature Secondary Structure
MRGKKSIISLFVAVLFILSLSFSGCSKSSNPISSQKVLADEQVLNINLGEEPPSLDPQLA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCHHHH
TDLVSFEMLNATLEGLVRLNKYGKIEKGSGLALDWEVSPDGLRYVFHLRDAKWSDGTPIT
HHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEE
AHDFEYAWKRALDPKTGSNYAYMLYYLKNGEAFNKGEAKAEDVGVRALNDKTLEVILERP
ECCHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHCCEEEECCCEEEHEECCC
APQFLGLTGWGTYLPAQKAAIEKFGDKYGTSPDTMVYSGPYMIKEWNHEQNIVLVKNPYY
CCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCEEEEECCCCCCEEEEECCCE
WDKDSVKLQQINMDMVKEENTIIQNWETGRYDSISVPAQYIAKYKNDPNFHQMPIGVTYY
ECCCCCEEEECCHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHCCCCCCEEECCCEEEE
VMFNNKSKIFSNVNIRKAFSYAIDRQSFVDYVFAGGGIPALAFVPPGIPGDKEEFRKEGG
EEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCHHHHHHCCC
DYFKDNDVAKAKELLAQGMRELGIDKLPKIKFVAGDSDAARKHSQVLQEFWSKNLGVTVD
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEE
IYNVAFKVRLDMMNKGDYDIVYTNWVADYNDPMTFLDLWVTDGGNNRAFYSNPQYDQLIK
EEEEEEEEEEEECCCCCEEEEEEEEECCCCCCEEEEEEEEECCCCCCEECCCCCHHHHHH
KANSTIDNSVGMQAMHEAENILMEDMPIAPLLFRITSYLERPYVKDWIRFPVGIDDEWKW
HHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEE
TYIEGKNK
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]