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The map label for this gene is AcoA [H]

Identifier: 20806712

GI number: 20806712

Start: 189702

End: 190703

Strand: Direct

Name: AcoA [H]

Synonym: TTE0186

Alternate gene names: 20806712

Gene position: 189702-190703 (Clockwise)

Preceding gene: 20806711

Following gene: 20806713

Centisome position: 7.05

GC content: 40.32

Gene sequence:

>1002_bases
GTGAAATTAAAAGGAGGTAGAATTGTGACTATGGAATTTTCAAAAGAAAAATTGCTGTGGATGTACAAAAAAATGGTTCA
AATAAGACAGTTTGAGCTAAAGACAGATGAGCTTTATAAGAAAAATATGATATGGGGGACATACCACCTGTACGTGGGAG
AGGAAGCTGCTGCAGTTGGAGCTTGTGCCGCATTAGAGCCCACGGACTATATAACGAGTACTCACAGGGGACATGGGCAT
ACAATAGCAAAAGGTGCGGATTTGAAGAGGATGATGGCAGAGCTTCTTGGCAAAGAGACGGGGCATTGCAAAGGAAGAGG
AGGTTCAATGCACATAGCAGATATTTTTACAGGCCATTTGGGTGCGAATGGAATCGTAGCTGGAGGAATTCCCATAGCTA
CAGGCGCGGCTTTAGCTTCTAAATTAAGAGGAGATAAAAGAGTTACGTTATGCTTTTTTGGTGATGGTGCAGCAAATACA
GGAGCATTTAATGAATCTCTAAATATGGCAGCAATATGGCAATTACCAGTTGTATATTTCTGCGAGAATAATCAGTATGC
TATGTCTGCACCAGTAAATAAATTTACTGCTGTAAAAAATATTGCTGACAGAGCAGCATCTTACAATATGCCTGGTGTAA
TAGTAGATGGGAATGATGTGTTAGAGGTATACAAAGCGGTAAAAGAAGCAGTGGACAGAGCAAGGAAAGGTGAAGGGCCT
ACACTTATAGAAGCAAAGACTTATAGATGGTTTGGACATTCTAAGAGTGATCCTAGAGTATATAGGACTAGAGAAGAAGA
AGAGGAATGGAAGAAAAAAGATCCTATAAAGAGATTTGCAGAAAAACTGCTTTCAGAAGGAGTGGCAACAGAAGAGGAAT
TAAAGAAAATAGACGAAGAGGTTGCAAAAGAGATAGAAGAGGCCTATCAATTTGCAGTAGAAAGTCCTGAACCCAAGGTA
GAAGACCTGGCAAAATACGTTTATGCAGAGGAGGGAGAGTAA

Upstream 100 bases:

>100_bases
AAAACACCTGCTTATATACCCTCTAATGCCCTTCATCAGTTTGTAAACATGGGAGAAGAGGTACTTGAGCTTATATGTAT
AGTGCCAGAAGAAGGAGAAA

Downstream 100 bases:

>100_bases
AGATGGCAGAAAAGCTCTATATAGAAGCGCTGGCAGAAGCAATAAAAGAAGAGTTTGAAAGAGACCCTAACGTTTTTATG
ATGGGCGAGGATATAGGAAT

Product: thiamine pyrophosphate-dependent dehydrogenase, E1 component alpha subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKLKGGRIVTMEFSKEKLLWMYKKMVQIRQFELKTDELYKKNMIWGTYHLYVGEEAAAVGACAALEPTDYITSTHRGHGH
TIAKGADLKRMMAELLGKETGHCKGRGGSMHIADIFTGHLGANGIVAGGIPIATGAALASKLRGDKRVTLCFFGDGAANT
GAFNESLNMAAIWQLPVVYFCENNQYAMSAPVNKFTAVKNIADRAASYNMPGVIVDGNDVLEVYKAVKEAVDRARKGEGP
TLIEAKTYRWFGHSKSDPRVYRTREEEEEWKKKDPIKRFAEKLLSEGVATEEELKKIDEEVAKEIEEAYQFAVESPEPKV
EDLAKYVYAEEGE

Sequences:

>Translated_333_residues
MKLKGGRIVTMEFSKEKLLWMYKKMVQIRQFELKTDELYKKNMIWGTYHLYVGEEAAAVGACAALEPTDYITSTHRGHGH
TIAKGADLKRMMAELLGKETGHCKGRGGSMHIADIFTGHLGANGIVAGGIPIATGAALASKLRGDKRVTLCFFGDGAANT
GAFNESLNMAAIWQLPVVYFCENNQYAMSAPVNKFTAVKNIADRAASYNMPGVIVDGNDVLEVYKAVKEAVDRARKGEGP
TLIEAKTYRWFGHSKSDPRVYRTREEEEEWKKKDPIKRFAEKLLSEGVATEEELKKIDEEVAKEIEEAYQFAVESPEPKV
EDLAKYVYAEEGE
>Mature_333_residues
MKLKGGRIVTMEFSKEKLLWMYKKMVQIRQFELKTDELYKKNMIWGTYHLYVGEEAAAVGACAALEPTDYITSTHRGHGH
TIAKGADLKRMMAELLGKETGHCKGRGGSMHIADIFTGHLGANGIVAGGIPIATGAALASKLRGDKRVTLCFFGDGAANT
GAFNESLNMAAIWQLPVVYFCENNQYAMSAPVNKFTAVKNIADRAASYNMPGVIVDGNDVLEVYKAVKEAVDRARKGEGP
TLIEAKTYRWFGHSKSDPRVYRTREEEEEWKKKDPIKRFAEKLLSEGVATEEELKKIDEEVAKEIEEAYQFAVESPEPKV
EDLAKYVYAEEGE

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=332, Percent_Identity=42.7710843373494, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI4505685, Length=332, Percent_Identity=43.3734939759036, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI291084742, Length=332, Percent_Identity=43.3734939759036, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI291084744, Length=339, Percent_Identity=42.4778761061947, Blast_Score=251, Evalue=4e-67,
Organism=Homo sapiens, GI291084757, Length=332, Percent_Identity=38.855421686747, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI11386135, Length=320, Percent_Identity=35.9375, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI258645172, Length=320, Percent_Identity=35.9375, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17536047, Length=337, Percent_Identity=41.2462908011869, Blast_Score=237, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI32564172, Length=337, Percent_Identity=41.2462908011869, Blast_Score=237, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI86563355, Length=321, Percent_Identity=33.6448598130841, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI86563357, Length=321, Percent_Identity=33.6448598130841, Blast_Score=160, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6321026, Length=329, Percent_Identity=41.0334346504559, Blast_Score=240, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24639748, Length=331, Percent_Identity=40.1812688821752, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24639740, Length=335, Percent_Identity=40.2985074626866, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24639744, Length=335, Percent_Identity=40.2985074626866, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI28571106, Length=335, Percent_Identity=40.2985074626866, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24639746, Length=312, Percent_Identity=41.6666666666667, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI21355903, Length=302, Percent_Identity=30.1324503311258, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 37060; Mature: 37060

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKGGRIVTMEFSKEKLLWMYKKMVQIRQFELKTDELYKKNMIWGTYHLYVGEEAAAVG
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEEEEEECCHHHHHH
ACAALEPTDYITSTHRGHGHTIAKGADLKRMMAELLGKETGHCKGRGGSMHIADIFTGHL
HHHCCCCHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHCCC
GANGIVAGGIPIATGAALASKLRGDKRVTLCFFGDGAANTGAFNESLNMAAIWQLPVVYF
CCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCEEEE
CENNQYAMSAPVNKFTAVKNIADRAASYNMPGVIVDGNDVLEVYKAVKEAVDRARKGEGP
ECCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCC
TLIEAKTYRWFGHSKSDPRVYRTREEEEEWKKKDPIKRFAEKLLSEGVATEEELKKIDEE
EEEEEEEEEECCCCCCCCCEECCCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
VAKEIEEAYQFAVESPEPKVEDLAKYVYAEEGE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLKGGRIVTMEFSKEKLLWMYKKMVQIRQFELKTDELYKKNMIWGTYHLYVGEEAAAVG
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEEEEEECCHHHHHH
ACAALEPTDYITSTHRGHGHTIAKGADLKRMMAELLGKETGHCKGRGGSMHIADIFTGHL
HHHCCCCHHHHHCCCCCCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHCCC
GANGIVAGGIPIATGAALASKLRGDKRVTLCFFGDGAANTGAFNESLNMAAIWQLPVVYF
CCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCEEEE
CENNQYAMSAPVNKFTAVKNIADRAASYNMPGVIVDGNDVLEVYKAVKEAVDRARKGEGP
ECCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCC
TLIEAKTYRWFGHSKSDPRVYRTREEEEEWKKKDPIKRFAEKLLSEGVATEEELKKIDEE
EEEEEEEEEECCCCCCCCCEECCCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
VAKEIEEAYQFAVESPEPKVEDLAKYVYAEEGE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]