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The map label for this gene is KsgA

Identifier: 20806642

GI number: 20806642

Start: 115504

End: 116310

Strand: Direct

Name: KsgA

Synonym: TTE0113

Alternate gene names: 20806642

Gene position: 115504-116310 (Clockwise)

Preceding gene: 20806641

Following gene: 20806643

Centisome position: 4.29

GC content: 36.43

Gene sequence:

>807_bases
ATGAAGGCGAAAAAGAAATGGGGTCAAAATTTCATATTTGACAAAAATCTTCTTTCAAAAATTGTGAGAGCTTCAGGAGT
AGGAGAAGAGGACTTTGTGCTAGAAGTGGGTACAGGGCATGGGGGGCTGACAGAGGAACTTGCCAAAAAGGTTAAAAAAG
TTGTATCCTTTGAAATAGATAAAGAGCTTTTTGAGATGAGTAGAGAAAAATTAAAAATCTATAAAAATGTGGTCATCATA
AATGAAGACATACTAGAAGTTGACCTTTTAGAAATTGCTCAGGAGCATTTTGACGGAAATTCCTTTAAAGTCGTAGCCAA
TCTCCCCTATTACATAACTTCACCTATAATAATGAAAATGCTTGACTGCAAATTGGTAAAAGAGATGACTGTGCTTGTTC
AAAAAGAAGTTGCTGAGAGAATATGCGCTTTACCAGGCACAAAGGACTACGGTATGCTTACTGTTTTTGTAAATTTCAAA
GCAAAACCGGAAATACTCTTTAACCTTCCTCCGAAGGTGTTTGTGCCTCCTCCGAAAGTGGAGTCTTCTCTTTTGAAATT
GAAAGTTTACGATAAGCCTTTAGTAGAAGTTAAAGACGAAAAGCTCTTTTCTGAAGTTGTAAGAGCAGCTTTTGGACAAA
GGAGAAAAGTTTTGTCCAACTCTTTAAAAGTTCTCGGCTTTTCAAAAGAAGTTTTACATGAAACTCTTTTAAAAGTTGGA
ATTTCCCCTCAGGCAAGGGGGGAAACCCTTTCAATAGATCAGTTTGCGAATTTGGCAAATGCACTTTATCTTCTCATAAA
AGAGTAA

Upstream 100 bases:

>100_bases
CTACCAGAGAAGAGGTCAGAAACTGGGGGAGAAGATACGTCAAAGTGTACATTCTTCAGTAGGGCAGGGGCTTCCCTGCC
TGATTTTTTGAGGTGAAGAT

Downstream 100 bases:

>100_bases
TAATATTTCCCCTCATTTCATAATATGTAGTGCGGCAATGGATTTTTAGTGAAATGAGGGGGATTAAGCAAATGCCAGTG
AATATATACAAGCGTTCTTA

Product: dimethyladenosine transferase (rRNA methylation)

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKAKKKWGQNFIFDKNLLSKIVRASGVGEEDFVLEVGTGHGGLTEELAKKVKKVVSFEIDKELFEMSREKLKIYKNVVII
NEDILEVDLLEIAQEHFDGNSFKVVANLPYYITSPIIMKMLDCKLVKEMTVLVQKEVAERICALPGTKDYGMLTVFVNFK
AKPEILFNLPPKVFVPPPKVESSLLKLKVYDKPLVEVKDEKLFSEVVRAAFGQRRKVLSNSLKVLGFSKEVLHETLLKVG
ISPQARGETLSIDQFANLANALYLLIKE

Sequences:

>Translated_268_residues
MKAKKKWGQNFIFDKNLLSKIVRASGVGEEDFVLEVGTGHGGLTEELAKKVKKVVSFEIDKELFEMSREKLKIYKNVVII
NEDILEVDLLEIAQEHFDGNSFKVVANLPYYITSPIIMKMLDCKLVKEMTVLVQKEVAERICALPGTKDYGMLTVFVNFK
AKPEILFNLPPKVFVPPPKVESSLLKLKVYDKPLVEVKDEKLFSEVVRAAFGQRRKVLSNSLKVLGFSKEVLHETLLKVG
ISPQARGETLSIDQFANLANALYLLIKE
>Mature_268_residues
MKAKKKWGQNFIFDKNLLSKIVRASGVGEEDFVLEVGTGHGGLTEELAKKVKKVVSFEIDKELFEMSREKLKIYKNVVII
NEDILEVDLLEIAQEHFDGNSFKVVANLPYYITSPIIMKMLDCKLVKEMTVLVQKEVAERICALPGTKDYGMLTVFVNFK
AKPEILFNLPPKVFVPPPKVESSLLKLKVYDKPLVEVKDEKLFSEVVRAAFGQRRKVLSNSLKVLGFSKEVLHETLLKVG
ISPQARGETLSIDQFANLANALYLLIKE

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=297, Percent_Identity=29.96632996633, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI7657198, Length=220, Percent_Identity=30.9090909090909, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=31.8007662835249, Blast_Score=147, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI25141369, Length=285, Percent_Identity=29.4736842105263, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI25146882, Length=282, Percent_Identity=29.4326241134752, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=221, Percent_Identity=28.0542986425339, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357273, Length=276, Percent_Identity=29.3478260869565, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21358017, Length=221, Percent_Identity=29.8642533936652, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_THETN (Q8RDC8)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_621813.1
- ProteinModelPortal:   Q8RDC8
- SMR:   Q8RDC8
- GeneID:   996444
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0113
- NMPDR:   fig|273068.3.peg.286
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   TTEN273068:TTE0113-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30287; Mature: 30287

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 10-10 BINDING 12-12 BINDING 37-37 BINDING 58-58 BINDING 83-83 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKKKWGQNFIFDKNLLSKIVRASGVGEEDFVLEVGTGHGGLTEELAKKVKKVVSFEID
CCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
KELFEMSREKLKIYKNVVIINEDILEVDLLEIAQEHFDGNSFKVVANLPYYITSPIIMKM
HHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHH
LDCKLVKEMTVLVQKEVAERICALPGTKDYGMLTVFVNFKAKPEILFNLPPKVFVPPPKV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEECCCCC
ESSLLKLKVYDKPLVEVKDEKLFSEVVRAAFGQRRKVLSNSLKVLGFSKEVLHETLLKVG
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHC
ISPQARGETLSIDQFANLANALYLLIKE
CCCCCCCCEECHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKAKKKWGQNFIFDKNLLSKIVRASGVGEEDFVLEVGTGHGGLTEELAKKVKKVVSFEID
CCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
KELFEMSREKLKIYKNVVIINEDILEVDLLEIAQEHFDGNSFKVVANLPYYITSPIIMKM
HHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHH
LDCKLVKEMTVLVQKEVAERICALPGTKDYGMLTVFVNFKAKPEILFNLPPKVFVPPPKV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEECCCCC
ESSLLKLKVYDKPLVEVKDEKLFSEVVRAAFGQRRKVLSNSLKVLGFSKEVLHETLLKVG
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHC
ISPQARGETLSIDQFANLANALYLLIKE
CCCCCCCCEECHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336