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The map label for this gene is PurF [H]

Identifier: 20095085

GI number: 20095085

Start: 1650476

End: 1651831

Strand: Reverse

Name: PurF [H]

Synonym: MK1649

Alternate gene names: 20095085

Gene position: 1651831-1650476 (Counterclockwise)

Preceding gene: 20095086

Following gene: 20095084

Centisome position: 97.45

GC content: 59.14

Gene sequence:

>1356_bases
TTGTGCGGGGTTTCCGGCTGTTACCTGCTTAAGGAAAACGAGGCGGGCGTTTATAATTACCTCATCCTCCATGCGAACCA
GCACAGGGGCCAAGAGTCGGCCGGAATCTGCGTGTACGATGGACTCCGACTGGTCGGGAAGAAGGGCATGGGCCTAGTGA
CCGAAGTCTTCGATCGGCCCGACCTGCGGAAGCTCTCGGGTCCAGTGGGTATCGGCCACGTCAGGTACTCGACGACTGGC
GCTTCCGAGCTCGTCAACGCCCAACCGTTCAAAGTGGGGTACTCGAAGGGCGAGCTGGCTTTGGCCCACAACGGAGACAT
CGTGAACTCCGAAGAGCTAAGGCGGGAGCTCGTTTCCGACGGACATGCTTTCGTTTCGGAGACTGATTCGGAGGTCATCG
CCCGACTGCTCGCGGTCGCTCTCACCGAGACCGACGATATGTTCGAGGCGTTCGAGGACGTTATGGAACGGTTGGTAGGC
TCGTACTCCCTGACCGTGATAACGAGTCACGGTGACCTAATCGCGGTCCGAGATCCCTGGGGATTCCGACCACTGTGCCT
GGGATGGGACGAACGTGGCTTCTTCGTTTCCTCCGAGACGGTAGGTCTGGACGTGTTAGGTGTGGAGGAACGCCGCGAGC
TGGAACGTGGCGAGGTAGTTTGGATTCGGGAGGGCGACGTCGAAAGTAAAGTCGTGAGAAGGGAACGCAAGGCCGTCTGT
ATGTTCGAGTTCGTGTACTTCGCACGGCCGGATTCCATCATCGAAGGCAGGTGCGTGTACGAATGTCGGAAGTGCATGGG
TAAGCGACTCGCCGAGGAGGCCCCGGTCGAGTGCGATCTGGTGGTTCCCGTCCCGGATTCGGGTCGGACGGCGGCGCTGG
GATACGCGGAGTCGTTGGGGGTCCCTATGGAAGAGGGACTGATCAAAAATCGGTACGTGGGTAGGACGTTCATCATGCCG
GAGCAGGAGGAGCGCGTACGGAGCATCCGGGTAAAGCTGAACCCCATCCGGGAGGTGATCAAAGGGTGCTCGCTGGCAGT
AGTGGACGACAGCATCGTACGTGGAAACACCTCTCGACAAATAGTGGAAATGCTGCGGGATGCCGGGGCCCGTGAGGTTC
ACATGAGGATCGCGTCACCTCCCGTGGTGTCCCCTTGTTACTACGGGATCGACATGGCCACGAAGGAGGAGCTAATAGCC
GCAGACCTCGATGTTCCAGAGATCTGTGAGAAAATCTCGGCAGACTCGCTGGCTTACCTGTCTCTAGAAGGTCTCGTCGA
GTCGATCAGATTGAAGAAACGGGAGCTGTGCGTGGGTTGCCTCACGGGGGAGTACCCCACCCCGGTCCCCGAATGA

Upstream 100 bases:

>100_bases
CCGGCTCGACCGCGTTGACCGGGTACGATATGAGCGACCACAGAAGGACCGCTACGGTCAGAACCGTGCCCATGTAGGGT
ACCGCCCGGGGGTGTAGTGG

Downstream 100 bases:

>100_bases
CCGGGGGTAAGAGGGGGTGGAACCCCGCGTCTATAGACCCCCGGCTGTATCAGCGTACCGGATTCCTCATAGTCGCGACA
CTTAACCTCGTAATCGTCGG

Product: glutamine phosphoribosylpyrophosphate amidotransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MCGVSGCYLLKENEAGVYNYLILHANQHRGQESAGICVYDGLRLVGKKGMGLVTEVFDRPDLRKLSGPVGIGHVRYSTTG
ASELVNAQPFKVGYSKGELALAHNGDIVNSEELRRELVSDGHAFVSETDSEVIARLLAVALTETDDMFEAFEDVMERLVG
SYSLTVITSHGDLIAVRDPWGFRPLCLGWDERGFFVSSETVGLDVLGVEERRELERGEVVWIREGDVESKVVRRERKAVC
MFEFVYFARPDSIIEGRCVYECRKCMGKRLAEEAPVECDLVVPVPDSGRTAALGYAESLGVPMEEGLIKNRYVGRTFIMP
EQEERVRSIRVKLNPIREVIKGCSLAVVDDSIVRGNTSRQIVEMLRDAGAREVHMRIASPPVVSPCYYGIDMATKEELIA
ADLDVPEICEKISADSLAYLSLEGLVESIRLKKRELCVGCLTGEYPTPVPE

Sequences:

>Translated_451_residues
MCGVSGCYLLKENEAGVYNYLILHANQHRGQESAGICVYDGLRLVGKKGMGLVTEVFDRPDLRKLSGPVGIGHVRYSTTG
ASELVNAQPFKVGYSKGELALAHNGDIVNSEELRRELVSDGHAFVSETDSEVIARLLAVALTETDDMFEAFEDVMERLVG
SYSLTVITSHGDLIAVRDPWGFRPLCLGWDERGFFVSSETVGLDVLGVEERRELERGEVVWIREGDVESKVVRRERKAVC
MFEFVYFARPDSIIEGRCVYECRKCMGKRLAEEAPVECDLVVPVPDSGRTAALGYAESLGVPMEEGLIKNRYVGRTFIMP
EQEERVRSIRVKLNPIREVIKGCSLAVVDDSIVRGNTSRQIVEMLRDAGAREVHMRIASPPVVSPCYYGIDMATKEELIA
ADLDVPEICEKISADSLAYLSLEGLVESIRLKKRELCVGCLTGEYPTPVPE
>Mature_451_residues
MCGVSGCYLLKENEAGVYNYLILHANQHRGQESAGICVYDGLRLVGKKGMGLVTEVFDRPDLRKLSGPVGIGHVRYSTTG
ASELVNAQPFKVGYSKGELALAHNGDIVNSEELRRELVSDGHAFVSETDSEVIARLLAVALTETDDMFEAFEDVMERLVG
SYSLTVITSHGDLIAVRDPWGFRPLCLGWDERGFFVSSETVGLDVLGVEERRELERGEVVWIREGDVESKVVRRERKAVC
MFEFVYFARPDSIIEGRCVYECRKCMGKRLAEEAPVECDLVVPVPDSGRTAALGYAESLGVPMEEGLIKNRYVGRTFIMP
EQEERVRSIRVKLNPIREVIKGCSLAVVDDSIVRGNTSRQIVEMLRDAGAREVHMRIASPPVVSPCYYGIDMATKEELIA
ADLDVPEICEKISADSLAYLSLEGLVESIRLKKRELCVGCLTGEYPTPVPE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=503, Percent_Identity=40.7554671968191, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI4826742, Length=202, Percent_Identity=33.1683168316832, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI205277386, Length=213, Percent_Identity=29.1079812206573, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788651, Length=463, Percent_Identity=39.3088552915767, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1790167, Length=289, Percent_Identity=31.1418685121107, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554892, Length=485, Percent_Identity=41.0309278350515, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17539970, Length=200, Percent_Identity=29, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532897, Length=200, Percent_Identity=29.5, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17532899, Length=200, Percent_Identity=29.5, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323958, Length=449, Percent_Identity=39.8663697104677, Blast_Score=289, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6322745, Length=211, Percent_Identity=30.3317535545024, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24659598, Length=465, Percent_Identity=46.0215053763441, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24659604, Length=465, Percent_Identity=46.0215053763441, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28573187, Length=467, Percent_Identity=45.8244111349036, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 49994; Mature: 49994

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVSGCYLLKENEAGVYNYLILHANQHRGQESAGICVYDGLRLVGKKGMGLVTEVFDRP
CCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCEEEECCHHHCCCCCCCHHHHHHCCC
DLRKLSGPVGIGHVRYSTTGASELVNAQPFKVGYSKGELALAHNGDIVNSEELRRELVSD
CHHHHCCCCCCCEEEEECCCHHHHCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHC
GHAFVSETDSEVIARLLAVALTETDDMFEAFEDVMERLVGSYSLTVITSHGDLIAVRDPW
CCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCC
GFRPLCLGWDERGFFVSSETVGLDVLGVEERRELERGEVVWIREGDVESKVVRRERKAVC
CCCCEEECCCCCCEEEECCCCCEEEEECHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHH
MFEFVYFARPDSIIEGRCVYECRKCMGKRLAEEAPVECDLVVPVPDSGRTAALGYAESLG
HHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCHHHCC
VPMEEGLIKNRYVGRTFIMPEQEERVRSIRVKLNPIREVIKGCSLAVVDDSIVRGNTSRQ
CCHHHCCHHCCCCCEEEECCCHHHHHHHHHEEEHHHHHHHCCCEEEEECCHHHCCCCHHH
IVEMLRDAGAREVHMRIASPPVVSPCYYGIDMATKEELIAADLDVPEICEKISADSLAYL
HHHHHHHCCCCEEEEEECCCCCCCCHHCCCCCCCHHHHHEECCCHHHHHHHHCCCCEEEH
SLEGLVESIRLKKRELCVGCLTGEYPTPVPE
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
>Mature Secondary Structure
MCGVSGCYLLKENEAGVYNYLILHANQHRGQESAGICVYDGLRLVGKKGMGLVTEVFDRP
CCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCEEEECCHHHCCCCCCCHHHHHHCCC
DLRKLSGPVGIGHVRYSTTGASELVNAQPFKVGYSKGELALAHNGDIVNSEELRRELVSD
CHHHHCCCCCCCEEEEECCCHHHHCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHC
GHAFVSETDSEVIARLLAVALTETDDMFEAFEDVMERLVGSYSLTVITSHGDLIAVRDPW
CCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCC
GFRPLCLGWDERGFFVSSETVGLDVLGVEERRELERGEVVWIREGDVESKVVRRERKAVC
CCCCEEECCCCCCEEEECCCCCEEEEECHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHH
MFEFVYFARPDSIIEGRCVYECRKCMGKRLAEEAPVECDLVVPVPDSGRTAALGYAESLG
HHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCHHHCC
VPMEEGLIKNRYVGRTFIMPEQEERVRSIRVKLNPIREVIKGCSLAVVDDSIVRGNTSRQ
CCHHHCCHHCCCCCEEEECCCHHHHHHHHHEEEHHHHHHHCCCEEEEECCHHHCCCCHHH
IVEMLRDAGAREVHMRIASPPVVSPCYYGIDMATKEELIAADLDVPEICEKISADSLAYL
HHHHHHHCCCCEEEEEECCCCCCCCHHCCCCCCCHHHHHEECCCHHHHHHHHCCCCEEEH
SLEGLVESIRLKKRELCVGCLTGEYPTPVPE
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]