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The map label for this gene is MoaA

Identifier: 20094976

GI number: 20094976

Start: 1557946

End: 1558869

Strand: Reverse

Name: MoaA

Synonym: MK1540

Alternate gene names: 20094976

Gene position: 1558869-1557946 (Counterclockwise)

Preceding gene: 20094977

Following gene: 20094975

Centisome position: 91.97

GC content: 60.28

Gene sequence:

>924_bases
TTGCGCGACGCGCTAGGTCGCGAGGTCCGAAGCGTTAGGATATCCGTAACGATGCGATGCAACATGGCCTGCGTGTACTG
CCACAGGGAAGGGGAGCGACCCGGGAGATCCGAGCTGAGCGCCGCCGAGTGGGGCCGACTGCTCCGAGCCTGTGCTGAGA
TCGGCGTTAGGAAGGTCAAGATTACCGGAGGTGAACCGTTACTCCGGAGGGATTTGATCGAGATAATCGAAAACGCCGAG
GGCTTCGAGGAGGTTTCCCTCGTCACGAACGGTGTACTGCTCGCGGATTACGCAGGTGACCTGGCCGAGGCCGGACTGGA
CCGCGTGAACGTGAGCCTCGACACGGTGGATCGAAAGTTGTACCGTAAGCTCACCCGGTCTCGTTTCTCTCCCGACGATG
TCATCAGAGGGATAGAGGCGGCGGTGTCGGAGGGATTGACCCCCGTCAAGGTGAACGTTGTGCTGACCTCGGAGACCGTG
AAGACGCTGCCGACGCTCGTGGAGGAGCTGGCCGACTTAGAGGGTCTGAAGCTGCAGCTGATCGAGCCGATGGGTTCGAT
ACCTGGATTTAGGCCCGCACATGCCGAGGACGGCCTGAGAGCGCTCGGTGAGTACGAACCGGAGCTGGAGCGGGTTCGCA
CGTTCCACAGCCGCGAGGTATACCGGCTTAACAACGGTATGGCCGTCGAAGTCGTCAAACCCATGGACGGGGTCATGTGT
GAGGCCTGCACTCGCATCCGTTTAACCCACGATGGAAAGTACAAGGGGTGCTTGATGGCACCTCCCAAACCGCTTCCTCG
GGATGACTTCGGAGAACTCGTTCGCACGCTGAAGGAGTACGTTCGAACTCGGGACGATACGTTCGAAGTACATCAAGGGA
CGTCGGTGATGGGGCGGATGCGCGGTGACGTCTCGGGACGCTAA

Upstream 100 bases:

>100_bases
AAATAGGCTTCAAGGGGAAGCCGAAGATCGGTGGGATCATATTCTGCAACATCTACGCCCTCCTACCCGCTTAATCACAT
TTTTCGGTGGGGTCCCTCGC

Downstream 100 bases:

>100_bases
GACCTACGAGGAAGCCGTGGAGATAGTGCTCCGTACGCTGGTGAAAGCGGAGAGGATCAAACCCACGCAGTACGCCAGAC
GTCTGGTGATGAAGGAGATG

Product: molybdenum cofactor biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRDALGREVRSVRISVTMRCNMACVYCHREGERPGRSELSAAEWGRLLRACAEIGVRKVKITGGEPLLRRDLIEIIENAE
GFEEVSLVTNGVLLADYAGDLAEAGLDRVNVSLDTVDRKLYRKLTRSRFSPDDVIRGIEAAVSEGLTPVKVNVVLTSETV
KTLPTLVEELADLEGLKLQLIEPMGSIPGFRPAHAEDGLRALGEYEPELERVRTFHSREVYRLNNGMAVEVVKPMDGVMC
EACTRIRLTHDGKYKGCLMAPPKPLPRDDFGELVRTLKEYVRTRDDTFEVHQGTSVMGRMRGDVSGR

Sequences:

>Translated_307_residues
MRDALGREVRSVRISVTMRCNMACVYCHREGERPGRSELSAAEWGRLLRACAEIGVRKVKITGGEPLLRRDLIEIIENAE
GFEEVSLVTNGVLLADYAGDLAEAGLDRVNVSLDTVDRKLYRKLTRSRFSPDDVIRGIEAAVSEGLTPVKVNVVLTSETV
KTLPTLVEELADLEGLKLQLIEPMGSIPGFRPAHAEDGLRALGEYEPELERVRTFHSREVYRLNNGMAVEVVKPMDGVMC
EACTRIRLTHDGKYKGCLMAPPKPLPRDDFGELVRTLKEYVRTRDDTFEVHQGTSVMGRMRGDVSGR
>Mature_307_residues
MRDALGREVRSVRISVTMRCNMACVYCHREGERPGRSELSAAEWGRLLRACAEIGVRKVKITGGEPLLRRDLIEIIENAE
GFEEVSLVTNGVLLADYAGDLAEAGLDRVNVSLDTVDRKLYRKLTRSRFSPDDVIRGIEAAVSEGLTPVKVNVVLTSETV
KTLPTLVEELADLEGLKLQLIEPMGSIPGFRPAHAEDGLRALGEYEPELERVRTFHSREVYRLNNGMAVEVVKPMDGVMC
EACTRIRLTHDGKYKGCLMAPPKPLPRDDFGELVRTLKEYVRTRDDTFEVHQGTSVMGRMRGDVSGR

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI22027536, Length=277, Percent_Identity=31.0469314079422, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI116734668, Length=276, Percent_Identity=31.1594202898551, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786999, Length=325, Percent_Identity=28.9230769230769, Blast_Score=110, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI32566331, Length=289, Percent_Identity=31.4878892733564, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17561210, Length=322, Percent_Identity=31.055900621118, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566329, Length=279, Percent_Identity=31.8996415770609, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28574488, Length=281, Percent_Identity=33.0960854092527, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28574490, Length=281, Percent_Identity=33.0960854092527, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_METKA (Q8TV60)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614823.1
- ProteinModelPortal:   Q8TV60
- SMR:   Q8TV60
- GeneID:   1478135
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1540
- NMPDR:   fig|190192.1.peg.1536
- HOGENOM:   HBG529885
- OMA:   EMENSQF
- BioCyc:   MKAN190192:MK1540-MONOMER
- HAMAP:   MF_01225_A
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013485
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02668

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 34239; Mature: 34239

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 13-13 BINDING 26-26 BINDING 60-60 BINDING 64-64 BINDING 112-112 BINDING 150-150 BINDING 184-184

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDALGREVRSVRISVTMRCNMACVYCHREGERPGRSELSAAEWGRLLRACAEIGVRKVK
CCCHHHHHHHEEEEEEEEEECEEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEE
ITGGEPLLRRDLIEIIENAEGFEEVSLVTNGVLLADYAGDLAEAGLDRVNVSLDTVDRKL
EECCCHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEHHHHHHHHCCHHHEECHHHHHHHHH
YRKLTRSRFSPDDVIRGIEAAVSEGLTPVKVNVVLTSETVKTLPTLVEELADLEGLKLQL
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECHHHHHHHHHHHHHHCCCCCEEEE
IEPMGSIPGFRPAHAEDGLRALGEYEPELERVRTFHSREVYRLNNGMAVEVVKPMDGVMC
ECHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCEEEEEECCCCHHHH
EACTRIRLTHDGKYKGCLMAPPKPLPRDDFGELVRTLKEYVRTRDDTFEVHQGTSVMGRM
HHHHHEEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHH
RGDVSGR
CCCCCCC
>Mature Secondary Structure
MRDALGREVRSVRISVTMRCNMACVYCHREGERPGRSELSAAEWGRLLRACAEIGVRKVK
CCCHHHHHHHEEEEEEEEEECEEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEE
ITGGEPLLRRDLIEIIENAEGFEEVSLVTNGVLLADYAGDLAEAGLDRVNVSLDTVDRKL
EECCCHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEHHHHHHHHCCHHHEECHHHHHHHHH
YRKLTRSRFSPDDVIRGIEAAVSEGLTPVKVNVVLTSETVKTLPTLVEELADLEGLKLQL
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECHHHHHHHHHHHHHHCCCCCEEEE
IEPMGSIPGFRPAHAEDGLRALGEYEPELERVRTFHSREVYRLNNGMAVEVVKPMDGVMC
ECHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCEEEEEECCCCHHHH
EACTRIRLTHDGKYKGCLMAPPKPLPRDDFGELVRTLKEYVRTRDDTFEVHQGTSVMGRM
HHHHHEEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHH
RGDVSGR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014