Click here to switch to the map view.

The map label for this gene is mfnA

Identifier: 20094936

GI number: 20094936

Start: 1517569

End: 1518687

Strand: Reverse

Name: mfnA

Synonym: MK1500

Alternate gene names: 20094936

Gene position: 1518687-1517569 (Counterclockwise)

Preceding gene: 20094937

Following gene: 20094935

Centisome position: 89.6

GC content: 62.56

Gene sequence:

>1119_bases
GTGATCCTCCAGCGGGACTCGGATTACTCCGACGGTACCGTGCTCGGATCCATGTGCACCGAACCGCACCCCGTCGCCGC
CGAAGCGTTCGTGGCTGGGCTTCACGTCAACTTGGGTGATCCTTACCTCTTTCCGAACGCGTACCGCGCCGAGCGTGAGT
GCATCGGATGGTTGGCGGAGACGCTCCTCGACCACCCCGCCCCTGAGGAGGCCGAAGGTAGCATAGTATCGGGAGGCACC
GAAGCCAACATCCTGGCGGCGTACGCGGCTCGGGAGGTCACCGGTGGTAGGGAAATCATCGTCCCCGCCACTCGCCATTT
CTCCTTCGAGAAGGCTGCACGGATGTTACGTATGAAGCTCGTCGAGGCGCCGCTGAGGTCCGACTACACCGTGGATGTAG
ACGCCGTTCAGGACCTGATTTCCCGGGATACCGCGCTGATCGTCGGTATCGTCGGTACCACCGAGACGGGGTCCGTCGAC
GACATCGAGGCGCTGTCGGACGTGGCGGAGGACCACGGCGTTCCGCTTCACGTGGACGCGGCGTTCGGGGGCTTCACGGC
ACCGTTCCTCCGGGAGGAGTACCCACTACCACGTTTCGGCTTCGATCTGGAGGCGGTGGTATCTGTCACCGTCGATCCTC
ACAAAATGGGGCTGGTACCTCCGCCAGCGGGCGGCATCGTGTTCCGAGATGACGAGTTCCCGAAAGCTATCGAGGTGTAC
GCGCCTTACTTGTCGGGAGGTGGGGCGTCACAGTACACCATCACCGGCACACGACCCGGAGCCCCGGTTCTGGCGCTCTA
CGCGAACATATTGGAGCTCGGGGAAGAGGGGTACCGGAGGATAGCCTTCAGATGTTACGAAGAGACCCTGAAAGTCGCGG
AGAAGGCCAGGGAACTCGGCCTAGAGCTGGCCGTGGACCCGCCCCATCTTAACTTGGTGAATATCCGGTTGCCCGACCGA
GGGACCGCGGAACGACTGTTGCGGGAATCGGAGCGGGAGGGTTGGAAGATCTCAGTTTCCACCAAGCCTCTCGGAGTCAG
GATAGTGATGATGCCACACCTCGACGCGGAGACGGTCTCACGGTTCCTCGAGCTCGTCGCCAGGGTGCTGGGTGGATGA

Upstream 100 bases:

>100_bases
TCAAGGGTCTGAAGGCGTTGGTAGTGATCACCACCCCCATGCTGAGGAGGGCGAGGGACAAGGCGGAACGGCGGGGAGCC
GAATACCGGATCCAGTACGT

Downstream 100 bases:

>100_bases
TCGGACCCTTGGATCCCCTCCGAGACTACCTCCGAGAACTGCTGGGAGAAGGCTTCGTTCTAGTCGGACTCGGCAACCCG
TTGGCCGCGGATGACGGGTT

Product: pyridoxal-phosphate-dependent enzyme related to glutamate decarboxylase

Products: NA

Alternate protein names: TDC

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MILQRDSDYSDGTVLGSMCTEPHPVAAEAFVAGLHVNLGDPYLFPNAYRAERECIGWLAETLLDHPAPEEAEGSIVSGGT
EANILAAYAAREVTGGREIIVPATRHFSFEKAARMLRMKLVEAPLRSDYTVDVDAVQDLISRDTALIVGIVGTTETGSVD
DIEALSDVAEDHGVPLHVDAAFGGFTAPFLREEYPLPRFGFDLEAVVSVTVDPHKMGLVPPPAGGIVFRDDEFPKAIEVY
APYLSGGGASQYTITGTRPGAPVLALYANILELGEEGYRRIAFRCYEETLKVAEKARELGLELAVDPPHLNLVNIRLPDR
GTAERLLRESEREGWKISVSTKPLGVRIVMMPHLDAETVSRFLELVARVLGG

Sequences:

>Translated_372_residues
MILQRDSDYSDGTVLGSMCTEPHPVAAEAFVAGLHVNLGDPYLFPNAYRAERECIGWLAETLLDHPAPEEAEGSIVSGGT
EANILAAYAAREVTGGREIIVPATRHFSFEKAARMLRMKLVEAPLRSDYTVDVDAVQDLISRDTALIVGIVGTTETGSVD
DIEALSDVAEDHGVPLHVDAAFGGFTAPFLREEYPLPRFGFDLEAVVSVTVDPHKMGLVPPPAGGIVFRDDEFPKAIEVY
APYLSGGGASQYTITGTRPGAPVLALYANILELGEEGYRRIAFRCYEETLKVAEKARELGLELAVDPPHLNLVNIRLPDR
GTAERLLRESEREGWKISVSTKPLGVRIVMMPHLDAETVSRFLELVARVLGG
>Mature_372_residues
MILQRDSDYSDGTVLGSMCTEPHPVAAEAFVAGLHVNLGDPYLFPNAYRAERECIGWLAETLLDHPAPEEAEGSIVSGGT
EANILAAYAAREVTGGREIIVPATRHFSFEKAARMLRMKLVEAPLRSDYTVDVDAVQDLISRDTALIVGIVGTTETGSVD
DIEALSDVAEDHGVPLHVDAAFGGFTAPFLREEYPLPRFGFDLEAVVSVTVDPHKMGLVPPPAGGIVFRDDEFPKAIEVY
APYLSGGGASQYTITGTRPGAPVLALYANILELGEEGYRRIAFRCYEETLKVAEKARELGLELAVDPPHLNLVNIRLPDR
GTAERLLRESEREGWKISVSTKPLGVRIVMMPHLDAETVSRFLELVARVLGG

Specific function: Specifically catalyzes the decarboxylation of L-tyrosine to produce tyramine

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Archaeal L-tyrosine decarboxylase subfamily

Homologues:

Organism=Homo sapiens, GI31982936, Length=270, Percent_Identity=32.2222222222222, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1789934, Length=287, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787769, Length=287, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543922, Length=344, Percent_Identity=29.0697674418605, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17557272, Length=321, Percent_Identity=28.6604361370716, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25148342, Length=286, Percent_Identity=29.7202797202797, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320500, Length=279, Percent_Identity=29.0322580645161, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323905, Length=277, Percent_Identity=29.6028880866426, Blast_Score=108, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21355963, Length=316, Percent_Identity=25.3164556962025, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24654344, Length=316, Percent_Identity=25.3164556962025, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): MFNA_METKA (Q8TV92)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614783.1
- ProteinModelPortal:   Q8TV92
- SMR:   Q8TV92
- GeneID:   1478095
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1500
- NMPDR:   fig|190192.1.peg.1496
- HOGENOM:   HBG497871
- OMA:   ILGSMCT
- BioCyc:   MKAN190192:MK1500-MONOMER
- BRENDA:   4.1.1.25
- HAMAP:   MF_01610
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR020931
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11999
- TIGRFAMs:   TIGR03812

Pfam domain/function: PF00282 Pyridoxal_deC; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.1.1.25

Molecular weight: Translated: 40407; Mature: 40407

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00392 DDC_GAD_HDC_YDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILQRDSDYSDGTVLGSMCTEPHPVAAEAFVAGLHVNLGDPYLFPNAYRAERECIGWLAE
CCCCCCCCCCCCEEEHHHCCCCCCCHHHHHEEEEEEECCCCEECCCHHHHHHHHHHHHHH
TLLDHPAPEEAEGSIVSGGTEANILAAYAAREVTGGREIIVPATRHFSFEKAARMLRMKL
HHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCEEEEECHHCCCHHHHHHHHHHHH
VEAPLRSDYTVDVDAVQDLISRDTALIVGIVGTTETGSVDDIEALSDVAEDHGVPLHVDA
HHCCCCCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEC
AFGGFTAPFLREEYPLPRFGFDLEAVVSVTVDPHKMGLVPPPAGGIVFRDDEFPKAIEVY
CCCCCCCHHHHHCCCCCCCCCCCEEEEEEEECHHHCCCCCCCCCCEEEECCCCCCEEEEE
APYLSGGGASQYTITGTRPGAPVLALYANILELGEEGYRRIAFRCYEETLKVAEKARELG
EHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LELAVDPPHLNLVNIRLPDRGTAERLLRESEREGWKISVSTKPLGVRIVMMPHLDAETVS
CEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH
RFLELVARVLGG
HHHHHHHHHHCC
>Mature Secondary Structure
MILQRDSDYSDGTVLGSMCTEPHPVAAEAFVAGLHVNLGDPYLFPNAYRAERECIGWLAE
CCCCCCCCCCCCEEEHHHCCCCCCCHHHHHEEEEEEECCCCEECCCHHHHHHHHHHHHHH
TLLDHPAPEEAEGSIVSGGTEANILAAYAAREVTGGREIIVPATRHFSFEKAARMLRMKL
HHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCEEEEECHHCCCHHHHHHHHHHHH
VEAPLRSDYTVDVDAVQDLISRDTALIVGIVGTTETGSVDDIEALSDVAEDHGVPLHVDA
HHCCCCCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEC
AFGGFTAPFLREEYPLPRFGFDLEAVVSVTVDPHKMGLVPPPAGGIVFRDDEFPKAIEVY
CCCCCCCHHHHHCCCCCCCCCCCEEEEEEEECHHHCCCCCCCCCCEEEECCCCCCEEEEE
APYLSGGGASQYTITGTRPGAPVLALYANILELGEEGYRRIAFRCYEETLKVAEKARELG
EHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LELAVDPPHLNLVNIRLPDRGTAERLLRESEREGWKISVSTKPLGVRIVMMPHLDAETVS
CEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH
RFLELVARVLGG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014