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The map label for this gene is RPB10

Identifier: 20094914

GI number: 20094914

Start: 1499006

End: 1499224

Strand: Direct

Name: RPB10

Synonym: MK1478

Alternate gene names: 20094914

Gene position: 1499006-1499224 (Clockwise)

Preceding gene: 20094913

Following gene: 20094915

Centisome position: 88.44

GC content: 60.27

Gene sequence:

>219_bases
ATGATCATACCGATCAGGTGCTTCACCTGTGGGCGCCCCATCGCCCACCTATGGGAGAAGTACGTGGAGTTGATCGAAGA
GGAGGGAATGGAACCCGGGGAGGCACTGGACGAGCTAGGCGTAGATCGGTACTGTTGCAGGAGGATGTTCCTGTCCCACG
TCGACCTGCTCGAGGAATCCCTCCCCTACACGCCACCCCGGCTCGGAATGCCCCGCTAA

Upstream 100 bases:

>100_bases
GCTGAAAGGTGACCCGCGCCGTAAGGAGCCGAAGAAGTTCGGTGGCCGCGGTGCCAGGGCACGGAGACAGAAGAGCTACC
GTTAAGGCGGGTGTCCACGG

Downstream 100 bases:

>100_bases
ATTCTTAAACACGATCTTCTGGGAGTCCTCCGGTCCACGTCAAGGGGCCGTGGGGTAGCCTGGTCTATCCTCCGCGGCTG
GGGAGCCGCGGCCCGGAGGC

Product: DNA-directed RNA polymerase subunit N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 72; Mature: 72

Protein sequence:

>72_residues
MIIPIRCFTCGRPIAHLWEKYVELIEEEGMEPGEALDELGVDRYCCRRMFLSHVDLLEESLPYTPPRLGMPR

Sequences:

>Translated_72_residues
MIIPIRCFTCGRPIAHLWEKYVELIEEEGMEPGEALDELGVDRYCCRRMFLSHVDLLEESLPYTPPRLGMPR
>Mature_72_residues
MIIPIRCFTCGRPIAHLWEKYVELIEEEGMEPGEALDELGVDRYCCRRMFLSHVDLLEESLPYTPPRLGMPR

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG1644

COG function: function code K; DNA-directed RNA polymerase, subunit N (RpoN/RPB10)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeal rpoN/eukaryotic RPB10 RNA polymerase subunit family

Homologues:

Organism=Homo sapiens, GI10863925, Length=65, Percent_Identity=58.4615384615385, Blast_Score=86, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17509857, Length=65, Percent_Identity=60, Blast_Score=82, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324784, Length=65, Percent_Identity=52.3076923076923, Blast_Score=86, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24649759, Length=65, Percent_Identity=61.5384615384615, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPON_METKA (Q8TVB4)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614761.1
- ProteinModelPortal:   Q8TVB4
- SMR:   Q8TVB4
- GeneID:   1478073
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1478
- NMPDR:   fig|190192.1.peg.1474
- HOGENOM:   HBG391971
- OMA:   KYCCRRM
- ProtClustDB:   PRK04016
- BioCyc:   MKAN190192:MK1478-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00250
- InterPro:   IPR012287
- InterPro:   IPR000268
- InterPro:   IPR020789
- Gene3D:   G3DSA:1.10.10.60
- PANTHER:   PTHR23413:SF2
- PIRSF:   PIRSF005653
- ProDom:   PD006539

Pfam domain/function: PF01194 RNA_pol_N; SSF46924 RNA_pol_N/8_sub

EC number: =2.7.7.6

Molecular weight: Translated: 8423; Mature: 8423

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS01112 RNA_POL_N_8KD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
11.1 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
11.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIPIRCFTCGRPIAHLWEKYVELIEEEGMEPGEALDELGVDRYCCRRMFLSHVDLLEES
CCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LPYTPPRLGMPR
CCCCCCCCCCCC
>Mature Secondary Structure
MIIPIRCFTCGRPIAHLWEKYVELIEEEGMEPGEALDELGVDRYCCRRMFLSHVDLLEES
CCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LPYTPPRLGMPR
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014