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The map label for this gene is RpoA

Identifier: 20094910

GI number: 20094910

Start: 1496884

End: 1497711

Strand: Direct

Name: RpoA

Synonym: MK1474

Alternate gene names: 20094910

Gene position: 1496884-1497711 (Clockwise)

Preceding gene: 20094909

Following gene: 20094911

Centisome position: 88.31

GC content: 57.49

Gene sequence:

>828_bases
ATGAGTCCCTTGCGCGTCCGTCTCTACGACTACCGGAAAGCCGACGTCGAGCGCGCCACGTTCATCATCGAGGAAACGTC
CGCGGAGTTCGTCAATACTATCCGCCGCGCCCTTTACACACTCGTTCCCACCTTACGCATCGAGGAGGTCATCATCTACG
AGAACGACACACCGATGTACGACGAAATGCTGGCCCATCGACTGGGACTGATACCGCTGCGTGTCGACGACATCGACCAG
TTCGAGTTGCCCGACCTGTGCGACTGCGGTGGGAAAGGTTGCGAGAAGTGTCAGGTACGGGCGGAGCTCGAAGTAGAAGG
GCCCACCAAAGTCTACGCTCGGGATCTGAAGTTCGATCATCCTGATGTGGAACCGGCCTTCCCTGATACGCTTATCACCG
AGGTCGGGGAGGACCAACGGATCAGGCTCGAAGTTATCGCCGTACCCGGTCTGGGCCTCGAACACGCGAAGTGGAAGCCG
GTGAGCGCAGTCGGGTACAAAGGCCTCCCCGAGCTCGAGATCGACGAGGATAAACTCAAGGAAAAGAAGATCACGTACGA
GTGTCCCCAGGGTATTATCAGGATCGAGAACGGCGAAGTAGTCCATATCGATGAGGATCGTCTCCCCGAGTGTCGGATGT
ACAAGGAGTACGAGCGCGAGACCGATGGGGCCGTTCGTGTCCGTCTCCGTGATGACGCCTTCGTGTTCAACGTTGAGACC
GACGGCTCGATGTCGCTCGACACCGCGATCTTGAAAGCTCTCGACGCGATCGAGCACAAGCTCGAGTCTCTGAAGAAGAA
CTTGCAGAAGGAGGTGAGCGGAGAATGA

Upstream 100 bases:

>100_bases
CTCTAGCCCGTGCAGGCCTGAAGATCGGACGGATCGAGGACGTCACGCCGATCCCGCACGACGGTACCAGGAGGCCCGGA
GGTAAGAGAGGACGCAGGGT

Downstream 100 bases:

>100_bases
CTTGGGCGCCCACCGGACCCACTAACGTGGAACTAAGGAAACTGATCCGTGACCTGAAGAAGGCGGCATGTGAGTACAAC
GCGCCAGTCTGGCGAGATGT

Product: DNA-directed RNA polymerase alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSPLRVRLYDYRKADVERATFIIEETSAEFVNTIRRALYTLVPTLRIEEVIIYENDTPMYDEMLAHRLGLIPLRVDDIDQ
FELPDLCDCGGKGCEKCQVRAELEVEGPTKVYARDLKFDHPDVEPAFPDTLITEVGEDQRIRLEVIAVPGLGLEHAKWKP
VSAVGYKGLPELEIDEDKLKEKKITYECPQGIIRIENGEVVHIDEDRLPECRMYKEYERETDGAVRVRLRDDAFVFNVET
DGSMSLDTAILKALDAIEHKLESLKKNLQKEVSGE

Sequences:

>Translated_275_residues
MSPLRVRLYDYRKADVERATFIIEETSAEFVNTIRRALYTLVPTLRIEEVIIYENDTPMYDEMLAHRLGLIPLRVDDIDQ
FELPDLCDCGGKGCEKCQVRAELEVEGPTKVYARDLKFDHPDVEPAFPDTLITEVGEDQRIRLEVIAVPGLGLEHAKWKP
VSAVGYKGLPELEIDEDKLKEKKITYECPQGIIRIENGEVVHIDEDRLPECRMYKEYERETDGAVRVRLRDDAFVFNVET
DGSMSLDTAILKALDAIEHKLESLKKNLQKEVSGE
>Mature_274_residues
SPLRVRLYDYRKADVERATFIIEETSAEFVNTIRRALYTLVPTLRIEEVIIYENDTPMYDEMLAHRLGLIPLRVDDIDQF
ELPDLCDCGGKGCEKCQVRAELEVEGPTKVYARDLKFDHPDVEPAFPDTLITEVGEDQRIRLEVIAVPGLGLEHAKWKPV
SAVGYKGLPELEIDEDKLKEKKITYECPQGIIRIENGEVVHIDEDRLPECRMYKEYERETDGAVRVRLRDDAFVFNVETD
GSMSLDTAILKALDAIEHKLESLKKNLQKEVSGE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeal rpoD/eukaryotic RPB3 RNA polymerase subunit family

Homologues:

Organism=Homo sapiens, GI14702171, Length=278, Percent_Identity=31.294964028777, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI42560246, Length=265, Percent_Identity=27.1698113207547, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4759046, Length=176, Percent_Identity=31.8181818181818, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322168, Length=272, Percent_Identity=30.5147058823529, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325367, Length=286, Percent_Identity=31.1188811188811, Blast_Score=102, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20129237, Length=272, Percent_Identity=28.6764705882353, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI17137650, Length=275, Percent_Identity=25.4545454545455, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPOD_METKA (Q8TVB8)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614757.1
- ProteinModelPortal:   Q8TVB8
- SMR:   Q8TVB8
- GeneID:   1478069
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1474
- NMPDR:   fig|190192.1.peg.1470
- HOGENOM:   HBG539019
- OMA:   SFIFTVE
- BioCyc:   MKAN190192:MK1474-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00320
- InterPro:   IPR001514
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR022842
- Gene3D:   G3DSA:2.170.120.12
- SMART:   SM00662

Pfam domain/function: PF01000 RNA_pol_A_bac; PF01193 RNA_pol_L; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 31526; Mature: 31395

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00446 RNA_POL_D_30KD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLRVRLYDYRKADVERATFIIEETSAEFVNTIRRALYTLVPTLRIEEVIIYENDTPMY
CCCEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHH
DEMLAHRLGLIPLRVDDIDQFELPDLCDCGGKGCEKCQVRAELEVEGPTKVYARDLKFDH
HHHHHHHHCCCEEEECCCCCCCCCCHHCCCCCCCCEEEEEEEEEECCCHHEEEECCCCCC
PDVEPAFPDTLITEVGEDQRIRLEVIAVPGLGLEHAKWKPVSAVGYKGLPELEIDEDKLK
CCCCCCCCHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHH
EKKITYECPQGIIRIENGEVVHIDEDRLPECRMYKEYERETDGAVRVRLRDDAFVFNVET
HCCCEEECCCCEEEECCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCEEEEEEEC
DGSMSLDTAILKALDAIEHKLESLKKNLQKEVSGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPLRVRLYDYRKADVERATFIIEETSAEFVNTIRRALYTLVPTLRIEEVIIYENDTPMY
CCEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHH
DEMLAHRLGLIPLRVDDIDQFELPDLCDCGGKGCEKCQVRAELEVEGPTKVYARDLKFDH
HHHHHHHHCCCEEEECCCCCCCCCCHHCCCCCCCCEEEEEEEEEECCCHHEEEECCCCCC
PDVEPAFPDTLITEVGEDQRIRLEVIAVPGLGLEHAKWKPVSAVGYKGLPELEIDEDKLK
CCCCCCCCHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHH
EKKITYECPQGIIRIENGEVVHIDEDRLPECRMYKEYERETDGAVRVRLRDDAFVFNVET
HCCCEEECCCCEEEECCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCEEEEEEEC
DGSMSLDTAILKALDAIEHKLESLKKNLQKEVSGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014