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The map label for this gene is radA [H]

Identifier: 20094878

GI number: 20094878

Start: 1474919

End: 1475872

Strand: Direct

Name: radA [H]

Synonym: MK1442

Alternate gene names: 20094878

Gene position: 1474919-1475872 (Clockwise)

Preceding gene: 20094877

Following gene: 20094879

Centisome position: 87.02

GC content: 59.75

Gene sequence:

>954_bases
GTGAAGGAGAGGAAGCTGAGTGATCTGGACCTACTGCCCGATGAAACCGTCAAAAAGCTGGAAGAGAAGGGGATCGTGAC
GGTCGAGGACTTCATTTACGCCGATCCGAAGTACCTGTCGGAGGTGACGGGGATGTCTGAGCGGGATGTGGAGGACATCC
AGGAGGAGTTGAGGAATATCGACGTGGAATTCGAGACGCTGGAGAAGCTGGAACGGAAACGCAGGCGCATAACCACGGGC
TCCAGCGCGCTCGACGAGATCTTAGGAGGAGGCGTCCCGTGCGGGGAGTTGACAGAGTTCGCGGGCCCCTTCGGCTCGGG
TAAGTCCCAGATCGTGTTCCAGCTGTGCGTGAACGTACAACTACCGGAAGAGGAGGGCGGGCTGGAGTCCAAGGCGATCT
TCATCGATACGGAGGGAACCGTATCACCCGGTCGTATCAAGGGGATGGCGGAAGCCTTAGGGCTGGATCCCGGAGAGGCC
CTGCGCAACGTCTTCGTGACGCAGGTGAGATCCGTGGAAGAGCAGATGCGAGCCGCGGAGGAGGCGCACAAACTGTGCGA
GCGCGAGGATATCGGACTCGTCGTCATAGATTCCCTCACGGCGCACTTCCGCGCGGAGTACTCGAAGCTGGGGGACGTCA
GCGAGCGTCAGGCCAGACTGATGAAGCACGTGGATCAACTCAGGAACCTTGCCATGGACCACGACGTGGCCGTGGTCTTC
ACCAACCAAGTGCACGTCGATATCGAGGCCGCCACGAAGGGCAAGGGCAGACGTTACGAGCCCGTCGGAGGAACGATCGT
AGCCCACCAGGCCACGCATAGGATCATGCTCAGGAGAGCCAAAGGTGAGGTCCGAATCGCACGGATCATCGACTCACCGT
ACCTTCCACAGCGTGAGGCAGCGTTCAGGATCACGGAAGAAGGCATTCGGGACGTGGAATTCCCTGAGCGATGA

Upstream 100 bases:

>100_bases
GAGGACTACGATGCCGTCGCACTGCGGGCTAGGATACTCAACGAGGAGGACTTCAAACGCGAAATCCCGATCTTGGTCCG
GGAGCTGAAGGGGGAGGAGA

Downstream 100 bases:

>100_bases
GGACAGGCCCAATAGCCGAAACCCGATGACGAGCTCCCGGTCCCATCCGAGCCCTCGCGGGTGAAACGCTTATGGAGTTC
GATGTGGTGGTGGTCGGTGC

Product: DNA repair and recombination protein RadA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKERKLSDLDLLPDETVKKLEEKGIVTVEDFIYADPKYLSEVTGMSERDVEDIQEELRNIDVEFETLEKLERKRRRITTG
SSALDEILGGGVPCGELTEFAGPFGSGKSQIVFQLCVNVQLPEEEGGLESKAIFIDTEGTVSPGRIKGMAEALGLDPGEA
LRNVFVTQVRSVEEQMRAAEEAHKLCEREDIGLVVIDSLTAHFRAEYSKLGDVSERQARLMKHVDQLRNLAMDHDVAVVF
TNQVHVDIEAATKGKGRRYEPVGGTIVAHQATHRIMLRRAKGEVRIARIIDSPYLPQREAAFRITEEGIRDVEFPER

Sequences:

>Translated_317_residues
MKERKLSDLDLLPDETVKKLEEKGIVTVEDFIYADPKYLSEVTGMSERDVEDIQEELRNIDVEFETLEKLERKRRRITTG
SSALDEILGGGVPCGELTEFAGPFGSGKSQIVFQLCVNVQLPEEEGGLESKAIFIDTEGTVSPGRIKGMAEALGLDPGEA
LRNVFVTQVRSVEEQMRAAEEAHKLCEREDIGLVVIDSLTAHFRAEYSKLGDVSERQARLMKHVDQLRNLAMDHDVAVVF
TNQVHVDIEAATKGKGRRYEPVGGTIVAHQATHRIMLRRAKGEVRIARIIDSPYLPQREAAFRITEEGIRDVEFPER
>Mature_317_residues
MKERKLSDLDLLPDETVKKLEEKGIVTVEDFIYADPKYLSEVTGMSERDVEDIQEELRNIDVEFETLEKLERKRRRITTG
SSALDEILGGGVPCGELTEFAGPFGSGKSQIVFQLCVNVQLPEEEGGLESKAIFIDTEGTVSPGRIKGMAEALGLDPGEA
LRNVFVTQVRSVEEQMRAAEEAHKLCEREDIGLVVIDSLTAHFRAEYSKLGDVSERQARLMKHVDQLRNLAMDHDVAVVF
TNQVHVDIEAATKGKGRRYEPVGGTIVAHQATHRIMLRRAKGEVRIARIIDSPYLPQREAAFRITEEGIRDVEFPER

Specific function: Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules [H]

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family [H]

Homologues:

Organism=Homo sapiens, GI19924133, Length=301, Percent_Identity=39.5348837209302, Blast_Score=207, Evalue=7e-54,
Organism=Homo sapiens, GI23238219, Length=322, Percent_Identity=37.5776397515528, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI256017143, Length=304, Percent_Identity=37.828947368421, Blast_Score=187, Evalue=7e-48,
Organism=Homo sapiens, GI256017141, Length=304, Percent_Identity=37.828947368421, Blast_Score=187, Evalue=7e-48,
Organism=Homo sapiens, GI256017145, Length=219, Percent_Identity=38.3561643835616, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI10835029, Length=332, Percent_Identity=27.4096385542169, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI19924117, Length=332, Percent_Identity=27.4096385542169, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI46255039, Length=332, Percent_Identity=27.4096385542169, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI17402896, Length=347, Percent_Identity=30.2593659942363, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI153946430, Length=342, Percent_Identity=27.4853801169591, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI4885659, Length=342, Percent_Identity=27.4853801169591, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI153946427, Length=342, Percent_Identity=27.4853801169591, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI217416415, Length=302, Percent_Identity=25.4966887417219, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71997295, Length=299, Percent_Identity=36.7892976588629, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71997301, Length=298, Percent_Identity=36.9127516778523, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321027, Length=302, Percent_Identity=40.3973509933775, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6320942, Length=299, Percent_Identity=38.4615384615385, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6320207, Length=237, Percent_Identity=31.6455696202532, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17864108, Length=299, Percent_Identity=35.4515050167224, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24651285, Length=236, Percent_Identity=38.9830508474576, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24650551, Length=212, Percent_Identity=33.4905660377358, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136506, Length=248, Percent_Identity=33.0645161290323, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011938
- InterPro:   IPR016467
- InterPro:   IPR020588
- InterPro:   IPR010995
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00633 HHH; PF08423 Rad51 [H]

EC number: NA

Molecular weight: Translated: 35544; Mature: 35544

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERKLSDLDLLPDETVKKLEEKGIVTVEDFIYADPKYLSEVTGMSERDVEDIQEELRNI
CCCCCCCCCCCCCHHHHHHHHHCCCEEEHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHCC
DVEFETLEKLERKRRRITTGSSALDEILGGGVPCGELTEFAGPFGSGKSQIVFQLCVNVQ
CCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCC
LPEEEGGLESKAIFIDTEGTVSPGRIKGMAEALGLDPGEALRNVFVTQVRSVEEQMRAAE
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EAHKLCEREDIGLVVIDSLTAHFRAEYSKLGDVSERQARLMKHVDQLRNLAMDHDVAVVF
HHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TNQVHVDIEAATKGKGRRYEPVGGTIVAHQATHRIMLRRAKGEVRIARIIDSPYLPQREA
ECEEEEEEEECCCCCCCEECCCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
AFRITEEGIRDVEFPER
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKERKLSDLDLLPDETVKKLEEKGIVTVEDFIYADPKYLSEVTGMSERDVEDIQEELRNI
CCCCCCCCCCCCCHHHHHHHHHCCCEEEHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHCC
DVEFETLEKLERKRRRITTGSSALDEILGGGVPCGELTEFAGPFGSGKSQIVFQLCVNVQ
CCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCC
LPEEEGGLESKAIFIDTEGTVSPGRIKGMAEALGLDPGEALRNVFVTQVRSVEEQMRAAE
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EAHKLCEREDIGLVVIDSLTAHFRAEYSKLGDVSERQARLMKHVDQLRNLAMDHDVAVVF
HHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TNQVHVDIEAATKGKGRRYEPVGGTIVAHQATHRIMLRRAKGEVRIARIIDSPYLPQREA
ECEEEEEEEECCCCCCCEECCCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
AFRITEEGIRDVEFPER
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475; 9922255 [H]