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The map label for this gene is LeuC_1

Identifier: 20094876

GI number: 20094876

Start: 1472310

End: 1473566

Strand: Reverse

Name: LeuC_1

Synonym: MK1440

Alternate gene names: 20094876

Gene position: 1473566-1472310 (Counterclockwise)

Preceding gene: 20094880

Following gene: 20094874

Centisome position: 86.94

GC content: 63.17

Gene sequence:

>1257_bases
GTGGGGAAGACGATGGCCGAGAAGATACTGTCTCGAGCGTCCGGAGAAGACGCTGAGGCGGGGGATATCGTCGTCGCCAA
CATCGACGTGGCGATGGTCCACGATATCACGGGCCCGATCACGGTTCAGCGGCTCGAGGAGATGGGAGTCGAGCGCGTGT
GGGACCCGTCCAAGATCGTCGTGCTGTTCGACCATCAGGTGCCCGCGGACTCCGTGGAAGCCGCGGAGAACCACAAAATA
ATGCGGGAGTTCGTCGAAGAACAGGGTATCGAGCACTTCTACGACGTTCGGGAGGGTGTCTGTCACCAGGTCCTCCCCGA
GAAGGGTCACGTGCGTCCCGGGGACGTGATCGTGGGGGCCGACTCTCACACCTGCACCCACGGAGCGCTCGGAGCCTTCG
CCACCGGGATCGGTTCCACCGACATGGCGGCGGTCTTTGCGACCGGTAAGCTGTGGTTCCGCGTCCCGGAAACTTACCGC
GTAGAGATCACGGGAGAGCTTCCGGAAGGTGTGTACGCGAAGGATGTCGTGCTCAAGGTGACGGGAGAAATCGGTGCCGA
CGGTGCGACGTACATGGCGATCGAGTACCACGGCGAGGTCGTCCGAGAGATGTCCGTATCGGACCGTATGTGCCTGTGCA
ACATGGCGATCGAGATGGGAGCGAAGACGGGAATGGTTCCACCCGACGAGAAGACGCTCGAGTACGTCAAGAAGCGTGCG
GGTACGGAAGGGCGCCCGGTTGAGCCGGACCCGGACGCCCGGTACGAGGCCGAGCTGACCTTGGACGTGAGCGACCTCGA
GCCGCAGGTCGCGAAGCCGTTCTCTCCGGACAACGTGGTGCCCGTGGGGGAGGTCGAAGGTATCGCGATCGACCAAGTCT
TCATCGGTTCCTGTACCAACGGTCGGTACGAGGACCTGAAGGTTGCGGCGGAGGTACTTGAAGGCGAGGAGGTCCACGAC
GACGTCCGGCTGATCGTCATCCCGGCGTCGAGGGAAGTTTACCACCGGACGCTCAAGGACGGCGTGCTCGAAGTACTCCA
CGAGGCGGGGGCACTGATCTGCCCGCCGAACTGCGGTCCGTGCCTGGGTGGCCATATGGGAGTGCTAGCGGAAGGCGAGC
GATGCGTAGCGACGTCCAACAGGAACTTTCCGGGTAGGATGGGACACCGCGAGTCGGAAGTGTATCTAGCCAGTCCGGCG
ACGGCCGCCGCCAGCGCTATCGAGGGTGAGATCACCGACCCTCGTCCCTACCTCTAA

Upstream 100 bases:

>100_bases
GATCGCCCCTCTAGACCTTGGCACTTCCACCTGATATACGGGGCTCGGGTCCCCGATCCTTTTTCAACCGCCGCTCGGGG
GCGGCCGGGGGTCGAGCACC

Downstream 100 bases:

>100_bases
GGTACAGCCATGTGGACCCCATCAGGCCGATCGCGAACGCTCCCCACCACAGCATCCTCTCCCACCACGTGAACCCGAAC
CGGAGCCGTGTAACACCTGT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MGKTMAEKILSRASGEDAEAGDIVVANIDVAMVHDITGPITVQRLEEMGVERVWDPSKIVVLFDHQVPADSVEAAENHKI
MREFVEEQGIEHFYDVREGVCHQVLPEKGHVRPGDVIVGADSHTCTHGALGAFATGIGSTDMAAVFATGKLWFRVPETYR
VEITGELPEGVYAKDVVLKVTGEIGADGATYMAIEYHGEVVREMSVSDRMCLCNMAIEMGAKTGMVPPDEKTLEYVKKRA
GTEGRPVEPDPDARYEAELTLDVSDLEPQVAKPFSPDNVVPVGEVEGIAIDQVFIGSCTNGRYEDLKVAAEVLEGEEVHD
DVRLIVIPASREVYHRTLKDGVLEVLHEAGALICPPNCGPCLGGHMGVLAEGERCVATSNRNFPGRMGHRESEVYLASPA
TAAASAIEGEITDPRPYL

Sequences:

>Translated_418_residues
MGKTMAEKILSRASGEDAEAGDIVVANIDVAMVHDITGPITVQRLEEMGVERVWDPSKIVVLFDHQVPADSVEAAENHKI
MREFVEEQGIEHFYDVREGVCHQVLPEKGHVRPGDVIVGADSHTCTHGALGAFATGIGSTDMAAVFATGKLWFRVPETYR
VEITGELPEGVYAKDVVLKVTGEIGADGATYMAIEYHGEVVREMSVSDRMCLCNMAIEMGAKTGMVPPDEKTLEYVKKRA
GTEGRPVEPDPDARYEAELTLDVSDLEPQVAKPFSPDNVVPVGEVEGIAIDQVFIGSCTNGRYEDLKVAAEVLEGEEVHD
DVRLIVIPASREVYHRTLKDGVLEVLHEAGALICPPNCGPCLGGHMGVLAEGERCVATSNRNFPGRMGHRESEVYLASPA
TAAASAIEGEITDPRPYL
>Mature_417_residues
GKTMAEKILSRASGEDAEAGDIVVANIDVAMVHDITGPITVQRLEEMGVERVWDPSKIVVLFDHQVPADSVEAAENHKIM
REFVEEQGIEHFYDVREGVCHQVLPEKGHVRPGDVIVGADSHTCTHGALGAFATGIGSTDMAAVFATGKLWFRVPETYRV
EITGELPEGVYAKDVVLKVTGEIGADGATYMAIEYHGEVVREMSVSDRMCLCNMAIEMGAKTGMVPPDEKTLEYVKKRAG
TEGRPVEPDPDARYEAELTLDVSDLEPQVAKPFSPDNVVPVGEVEGIAIDQVFIGSCTNGRYEDLKVAAEVLEGEEVHDD
VRLIVIPASREVYHRTLKDGVLEVLHEAGALICPPNCGPCLGGHMGVLAEGERCVATSNRNFPGRMGHRESEVYLASPAT
AAASAIEGEITDPRPYL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=445, Percent_Identity=29.2134831460674, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI8659555, Length=459, Percent_Identity=23.7472766884532, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI41352693, Length=395, Percent_Identity=25.0632911392405, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1786259, Length=467, Percent_Identity=35.9743040685225, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI87081781, Length=447, Percent_Identity=26.1744966442953, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787531, Length=388, Percent_Identity=25.2577319587629, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI2367097, Length=131, Percent_Identity=35.1145038167939, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=445, Percent_Identity=28.0898876404494, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=411, Percent_Identity=28.2238442822384, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=29.2253521126761, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=485, Percent_Identity=25.360824742268, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320440, Length=446, Percent_Identity=32.9596412556054, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6321429, Length=484, Percent_Identity=32.4380165289256, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6323335, Length=449, Percent_Identity=28.9532293986637, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6322261, Length=373, Percent_Identity=27.6139410187668, Blast_Score=155, Evalue=8e-39,
Organism=Drosophila melanogaster, GI281365315, Length=445, Percent_Identity=27.8651685393258, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17864292, Length=445, Percent_Identity=27.8651685393258, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI161076999, Length=410, Percent_Identity=28.0487804878049, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI28571643, Length=418, Percent_Identity=28.4688995215311, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645686, Length=370, Percent_Identity=26.4864864864865, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=26.9541778975741, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC1_METKA (Q8TVF2)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614723.1
- ProteinModelPortal:   Q8TVF2
- GeneID:   1478035
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1440
- NMPDR:   fig|190192.1.peg.1436
- HOGENOM:   HBG330745
- OMA:   TDPRDFL
- ProtClustDB:   PRK00402
- BioCyc:   MKAN190192:MK1440-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 45203; Mature: 45071

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2; PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTMAEKILSRASGEDAEAGDIVVANIDVAMVHDITGPITVQRLEEMGVERVWDPSKIV
CCHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHHHCCHHHCCCCCEEE
VLFDHQVPADSVEAAENHKIMREFVEEQGIEHFYDVREGVCHQVLPEKGHVRPGDVIVGA
EEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEC
DSHTCTHGALGAFATGIGSTDMAAVFATGKLWFRVPETYRVEITGELPEGVYAKDVVLKV
CCCCCCCCCHHHHHHCCCCCCCEEEEEECEEEEECCCEEEEEEECCCCCCCEECEEEEEE
TGEIGADGATYMAIEYHGEVVREMSVSDRMCLCNMAIEMGAKTGMVPPDEKTLEYVKKRA
ECCCCCCCEEEEEEEECHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
GTEGRPVEPDPDARYEAELTLDVSDLEPQVAKPFSPDNVVPVGEVEGIAIDQVFIGSCTN
CCCCCCCCCCCCCCEEEEEEEEHHCCCCHHCCCCCCCCEEECCCCCCEEEEEEEEECCCC
GRYEDLKVAAEVLEGEEVHDDVRLIVIPASREVYHRTLKDGVLEVLHEAGALICPPNCGP
CCHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
CLGGHMGVLAEGERCVATSNRNFPGRMGHRESEVYLASPATAAASAIEGEITDPRPYL
CCCCCCCEEECCCEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GKTMAEKILSRASGEDAEAGDIVVANIDVAMVHDITGPITVQRLEEMGVERVWDPSKIV
CHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHHHCCHHHCCCCCEEE
VLFDHQVPADSVEAAENHKIMREFVEEQGIEHFYDVREGVCHQVLPEKGHVRPGDVIVGA
EEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEC
DSHTCTHGALGAFATGIGSTDMAAVFATGKLWFRVPETYRVEITGELPEGVYAKDVVLKV
CCCCCCCCCHHHHHHCCCCCCCEEEEEECEEEEECCCEEEEEEECCCCCCCEECEEEEEE
TGEIGADGATYMAIEYHGEVVREMSVSDRMCLCNMAIEMGAKTGMVPPDEKTLEYVKKRA
ECCCCCCCEEEEEEEECHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
GTEGRPVEPDPDARYEAELTLDVSDLEPQVAKPFSPDNVVPVGEVEGIAIDQVFIGSCTN
CCCCCCCCCCCCCCEEEEEEEEHHCCCCHHCCCCCCCCEEECCCCCCEEEEEEEEECCCC
GRYEDLKVAAEVLEGEEVHDDVRLIVIPASREVYHRTLKDGVLEVLHEAGALICPPNCGP
CCHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
CLGGHMGVLAEGERCVATSNRNFPGRMGHRESEVYLASPATAAASAIEGEITDPRPYL
CCCCCCCEEECCCEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014