Click here to switch to the map view.

The map label for this gene is TruA

Identifier: 20094324

GI number: 20094324

Start: 836360

End: 837130

Strand: Reverse

Name: TruA

Synonym: MK0888

Alternate gene names: 20094324

Gene position: 837130-836360 (Counterclockwise)

Preceding gene: 20094325

Following gene: 20094323

Centisome position: 49.39

GC content: 57.59

Gene sequence:

>771_bases
ATGAGAGTAGCCCTCCGGGTCGCCTACGACGGTTCGCGATATCACGGTTTCCAGTACCAACCCGACGTCCCAACGATCGA
AGGAGCGCTACGAAAAGCGCTCTCGGAGCTGGGATTGGAACTGGTAGGGTACGCATCCAGGACCGACGCGGGGGCCCATG
CACGCTACCAGGTAGTCGTGGTAGAAGGCGACCCGGAGCTAGCCCAACCGGACCCCATCAACGCGAGATTACCCAAGGAC
ATCCGGGTTATAGCCAAAACGGAAGTCCATGAAGAGTTCGATCCGCGGCGCGACGCGCTACGAAAGGAGTATCGGTACTT
CTTGGGACCACTAAACCGTCCCGAAGCCGCGGCACGGGCTGCCCGCAAGCTCGAAGGAAAACACGATTTTTCGGCATTCC
GAAGGGAGGATGGCCGGAATCCGATAATAACCGTCGAGCGGTGTGAACTCGTAGAAATAACCCCTAACGCGTACGTGTTG
CGGGTCGTGGCACCGCGATTCCTGTGGGAGATGGTCCGCAGGATAGCGGGGTTCGTGTGGGAGGTAGGCCACGGGCTCAG
GGAGGAAGGGGACGCCGAAGCGCTACTTTCTGGGAAGTTCGAGCCGTCGAAGAAACCCAGATGCCTTCCGGCGGAGGGAT
TGATCTTGTGGCACATCGAGTACGATGAGGTACGTTTTGAGCGTACCAAAGCTTGGTTTGAAGATCATAAAGTAATACAA
TTAGGGGGGAGATTACTACTACGCCTGCCCGAGGGGGCAGAAAGTTGCTGA

Upstream 100 bases:

>100_bases
ACGAACCCGTGATCCGTTTCGGAAACCGGGAACTAGTTTCCGAGGAGGAAATGATAGAGGTCTACCGGAGGCTCCCTTAC
AAGCCCCCTGGGGCCGGGCG

Downstream 100 bases:

>100_bases
AAGTCACCGTCTGCCCGCCGTGCTACGGGTTCAGAGAAGTATGCCATAAGTTAGCGCAAGACGGCGAAATCGTAGACGTA
TCGGATGCTCGAAGGCTATC

Product: TruD family pseudouridine synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRVALRVAYDGSRYHGFQYQPDVPTIEGALRKALSELGLELVGYASRTDAGAHARYQVVVVEGDPELAQPDPINARLPKD
IRVIAKTEVHEEFDPRRDALRKEYRYFLGPLNRPEAAARAARKLEGKHDFSAFRREDGRNPIITVERCELVEITPNAYVL
RVVAPRFLWEMVRRIAGFVWEVGHGLREEGDAEALLSGKFEPSKKPRCLPAEGLILWHIEYDEVRFERTKAWFEDHKVIQ
LGGRLLLRLPEGAESC

Sequences:

>Translated_256_residues
MRVALRVAYDGSRYHGFQYQPDVPTIEGALRKALSELGLELVGYASRTDAGAHARYQVVVVEGDPELAQPDPINARLPKD
IRVIAKTEVHEEFDPRRDALRKEYRYFLGPLNRPEAAARAARKLEGKHDFSAFRREDGRNPIITVERCELVEITPNAYVL
RVVAPRFLWEMVRRIAGFVWEVGHGLREEGDAEALLSGKFEPSKKPRCLPAEGLILWHIEYDEVRFERTKAWFEDHKVIQ
LGGRLLLRLPEGAESC
>Mature_256_residues
MRVALRVAYDGSRYHGFQYQPDVPTIEGALRKALSELGLELVGYASRTDAGAHARYQVVVVEGDPELAQPDPINARLPKD
IRVIAKTEVHEEFDPRRDALRKEYRYFLGPLNRPEAAARAARKLEGKHDFSAFRREDGRNPIITVERCELVEITPNAYVL
RVVAPRFLWEMVRRIAGFVWEVGHGLREEGDAEALLSGKFEPSKKPRCLPAEGLILWHIEYDEVRFERTKAWFEDHKVIQ
LGGRLLLRLPEGAESC

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI199559785, Length=270, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=33.469387755102, Blast_Score=92, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321112, Length=216, Percent_Identity=29.1666666666667, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325044, Length=121, Percent_Identity=38.8429752066116, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_METKA (Q8TWZ3)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614171.1
- ProteinModelPortal:   Q8TWZ3
- SMR:   Q8TWZ3
- GeneID:   1476989
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0888
- NMPDR:   fig|190192.1.peg.884
- HOGENOM:   HBG621020
- OMA:   CELVEIT
- BioCyc:   MKAN190192:MK0888-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29255; Mature: 29255

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: ACT_SITE 49-49

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVALRVAYDGSRYHGFQYQPDVPTIEGALRKALSELGLELVGYASRTDAGAHARYQVVV
CEEEEEEEECCCEECCEEECCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEEEE
VEGDPELAQPDPINARLPKDIRVIAKTEVHEEFDPRRDALRKEYRYFLGPLNRPEAAARA
EECCCCCCCCCCCCCCCCCHHEEEEHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHH
ARKLEGKHDFSAFRREDGRNPIITVERCELVEITPNAYVLRVVAPRFLWEMVRRIAGFVW
HHHHCCCHHHHHHHHHCCCCCEEEEECCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHH
EVGHGLREEGDAEALLSGKFEPSKKPRCLPAEGLILWHIEYDEVRFERTKAWFEDHKVIQ
HHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCEEE
LGGRLLLRLPEGAESC
ECCEEEEECCCCCCCC
>Mature Secondary Structure
MRVALRVAYDGSRYHGFQYQPDVPTIEGALRKALSELGLELVGYASRTDAGAHARYQVVV
CEEEEEEEECCCEECCEEECCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEEEE
VEGDPELAQPDPINARLPKDIRVIAKTEVHEEFDPRRDALRKEYRYFLGPLNRPEAAARA
EECCCCCCCCCCCCCCCCCHHEEEEHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHH
ARKLEGKHDFSAFRREDGRNPIITVERCELVEITPNAYVLRVVAPRFLWEMVRRIAGFVW
HHHHCCCHHHHHHHHHCCCCCEEEEECCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHH
EVGHGLREEGDAEALLSGKFEPSKKPRCLPAEGLILWHIEYDEVRFERTKAWFEDHKVIQ
HHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCEEE
LGGRLLLRLPEGAESC
ECCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014