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The map label for this gene is PurL_1

Identifier: 20094227

GI number: 20094227

Start: 750903

End: 751583

Strand: Reverse

Name: PurL_1

Synonym: MK0790

Alternate gene names: 20094227

Gene position: 751583-750903 (Counterclockwise)

Preceding gene: 20094228

Following gene: 20094222

Centisome position: 44.34

GC content: 59.77

Gene sequence:

>681_bases
TTGGTCCGTGTGGCTGTCATCAGGTTTCCCGGCACGAACTGCGACCTCGAGATGGCATGGGCCGTGAAGCTCGCTGGTGG
AGATCCGGAGTTCGTCTGGCACGAAGATGGTGGGTTAGACGACTTCGACGCCGTGATCATCCCGGGCGGATTCACTTACG
GGGATTACATCCGGGCGGGCGCCATCGCCGCGCTATCCCCGATTTTGGAGGAGATTCGAGAGTGTGCAGAGGACGGGCGC
CCCGTGCTGGGAGTGTGTAACGGAATGCAGATACTAGCCGAAGCGGAGCTCATCCCGGGTACTCTCACGGTCAACGTCGG
GAACAGGTTCATCTGCGATTGGGTCTACTTACGCGTGGAACGTACTGACACCCCGTTCACGACCAAGTACCAGGAGGACG
AAGTCATTCGAGTTCCGATCGCCCACGCGGAGGGACGGTACTACTACGAAAACCCGGAGGAGATCGAGGACAACGTGGTG
TTCAGGTTCTGCGGACCCGACGGCGACGTCTCAGAGGAGTACAACCTCAACGGCTCCGTCGGCGGTATTACGGGAGTTGT
GAACGACGATGGGAACGTCCTCGGAATGATGCCTCACCCTGAGCGCGCGGCTCACCGTCTCCTGAACTCCGATGACGGTT
TACGGTTGTTCGAGTCGCTCGTCGAGTGGTGCCGATCCTGA

Upstream 100 bases:

>100_bases
CGAAGACTACGAGATCGAGGTCGTCGAGTGAACCCCCTCGGCGTACTTGGGACTCCCCATCACCTCGGTCCCGGGGTAGG
TCATCATCGGGGGCCTGAGT

Downstream 100 bases:

>100_bases
TCCAGCAGCCAATCCAGGAGTATCGACACGGACTTGCGGGTATAACGTTCCACGTCCGACTGGTCCCACGGGTCGACACC
TCGGGCCAGTTTCACCAGCT

Product: phosphoribosylformylglycinamidine synthase I

Products: NA

Alternate protein names: Phosphoribosylformylglycinamidine synthase I; FGAM synthase I

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MVRVAVIRFPGTNCDLEMAWAVKLAGGDPEFVWHEDGGLDDFDAVIIPGGFTYGDYIRAGAIAALSPILEEIRECAEDGR
PVLGVCNGMQILAEAELIPGTLTVNVGNRFICDWVYLRVERTDTPFTTKYQEDEVIRVPIAHAEGRYYYENPEEIEDNVV
FRFCGPDGDVSEEYNLNGSVGGITGVVNDDGNVLGMMPHPERAAHRLLNSDDGLRLFESLVEWCRS

Sequences:

>Translated_226_residues
MVRVAVIRFPGTNCDLEMAWAVKLAGGDPEFVWHEDGGLDDFDAVIIPGGFTYGDYIRAGAIAALSPILEEIRECAEDGR
PVLGVCNGMQILAEAELIPGTLTVNVGNRFICDWVYLRVERTDTPFTTKYQEDEVIRVPIAHAEGRYYYENPEEIEDNVV
FRFCGPDGDVSEEYNLNGSVGGITGVVNDDGNVLGMMPHPERAAHRLLNSDDGLRLFESLVEWCRS
>Mature_226_residues
MVRVAVIRFPGTNCDLEMAWAVKLAGGDPEFVWHEDGGLDDFDAVIIPGGFTYGDYIRAGAIAALSPILEEIRECAEDGR
PVLGVCNGMQILAEAELIPGTLTVNVGNRFICDWVYLRVERTDTPFTTKYQEDEVIRVPIAHAEGRYYYENPEEIEDNVV
FRFCGPDGDVSEEYNLNGSVGGITGVVNDDGNVLGMMPHPERAAHRLLNSDDGLRLFESLVEWCRS

Specific function: Unknown

COG id: COG0047

COG function: function code F; Phosphoribosylformylglycinamidine (FGAM) synthase, glutamine amidotransferase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI31657129, Length=242, Percent_Identity=33.8842975206612, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI48994899, Length=233, Percent_Identity=35.1931330472103, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17553022, Length=233, Percent_Identity=31.3304721030043, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321498, Length=266, Percent_Identity=30.0751879699248, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24582111, Length=233, Percent_Identity=29.6137339055794, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24582109, Length=233, Percent_Identity=29.6137339055794, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17137292, Length=233, Percent_Identity=29.6137339055794, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURQ_METKA (Q8TX85)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614074.1
- ProteinModelPortal:   Q8TX85
- SMR:   Q8TX85
- GeneID:   1476891
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0790
- NMPDR:   fig|190192.1.peg.787
- HOGENOM:   HBG302712
- OMA:   FPGTNCD
- ProtClustDB:   PRK03619
- BioCyc:   MKAN190192:MK0790-MONOMER
- BRENDA:   6.3.5.3
- GO:   GO:0005737
- HAMAP:   MF_00421
- InterPro:   IPR017926
- InterPro:   IPR011698
- InterPro:   IPR010075
- PIRSF:   PIRSF001586
- TIGRFAMs:   TIGR01737

Pfam domain/function: PF07685 GATase_3

EC number: =6.3.5.3

Molecular weight: Translated: 25032; Mature: 25032

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 86-86 ACT_SITE 199-199 ACT_SITE 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRVAVIRFPGTNCDLEMAWAVKLAGGDPEFVWHEDGGLDDFDAVIIPGGFTYGDYIRAG
CEEEEEEEECCCCCCEEEEEEEEECCCCCCEEEECCCCCCCCCEEEECCCCCCHHHHHHH
AIAALSPILEEIRECAEDGRPVLGVCNGMQILAEAELIPGTLTVNVGNRFICDWVYLRVE
HHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEHEECCCEEEEECCCEEEEEEEEEEEE
RTDTPFTTKYQEDEVIRVPIAHAEGRYYYENPEEIEDNVVFRFCGPDGDVSEEYNLNGSV
CCCCCCEECCCCCCEEEEEEEECCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
GGITGVVNDDGNVLGMMPHPERAAHRLLNSDDGLRLFESLVEWCRS
CCEEEEEECCCCEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVRVAVIRFPGTNCDLEMAWAVKLAGGDPEFVWHEDGGLDDFDAVIIPGGFTYGDYIRAG
CEEEEEEEECCCCCCEEEEEEEEECCCCCCEEEECCCCCCCCCEEEECCCCCCHHHHHHH
AIAALSPILEEIRECAEDGRPVLGVCNGMQILAEAELIPGTLTVNVGNRFICDWVYLRVE
HHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEHEECCCEEEEECCCEEEEEEEEEEEE
RTDTPFTTKYQEDEVIRVPIAHAEGRYYYENPEEIEDNVVFRFCGPDGDVSEEYNLNGSV
CCCCCCEECCCCCCEEEEEEEECCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
GGITGVVNDDGNVLGMMPHPERAAHRLLNSDDGLRLFESLVEWCRS
CCEEEEEECCCCEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014