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The map label for this gene is Efp

Identifier: 20094176

GI number: 20094176

Start: 703117

End: 703527

Strand: Reverse

Name: Efp

Synonym: MK0739

Alternate gene names: 20094176

Gene position: 703527-703117 (Counterclockwise)

Preceding gene: 20094180

Following gene: 20094175

Centisome position: 41.51

GC content: 56.2

Gene sequence:

>411_bases
CTGGACGTGGCCGTGAAGAAAGTCGAGGTGCGGCAGCTTAAGAAGGGCAAGTACATCATGATCGACGACGAGCCGTGTAA
GATTGTGGAGTACACCACGTCCTCACCGGGTAAGCACGGTTCGGCGAAGGCGCGGATCGTAGCGGTAGGGCTGTTCGACG
GTAAGAAGCGCACGCTTACTAAACCGGTGGACGCGAAGGTGGATGTACCGGTGATCGAGCGGAAGACAGCCCAGGTAGTC
TCCGATATGGGTGATACGGTGCAGCTGATGGATATGGAAACCTACGAGACATTCGAAGTTCAGAAGCCGGAGGACGAGGA
ACTCGCCTCGCAGCTTGAACCGGGAACCATGGTGGAGTACATGGAGGCAGCGGGCAAGCGTAAAATTGTGGGAATCAAGG
AGGAAGAGTGA

Upstream 100 bases:

>100_bases
ACTAGGGCGTACTTCTTAGGTACTCCCAGCAATCCTATCCCCCTCGCGAAGCTTATTAGGAGGGATCTCGCTCGCGCCGT
GACACTCCGGGAAGGGGGTT

Downstream 100 bases:

>100_bases
ACCCCGCTTCCCACTCTCGTTTTCATCCGACGACGTGAAATCCCCGGATGTAGCCGTAGTCGGACTTCCCAGGGAGCGTG
TGCTCCCAGAGTACCCGACC

Product: translation initiation factor IF-5A

Products: NA

Alternate protein names: Hypusine-containing protein; eIF-5A

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MDVAVKKVEVRQLKKGKYIMIDDEPCKIVEYTTSSPGKHGSAKARIVAVGLFDGKKRTLTKPVDAKVDVPVIERKTAQVV
SDMGDTVQLMDMETYETFEVQKPEDEELASQLEPGTMVEYMEAAGKRKIVGIKEEE

Sequences:

>Translated_136_residues
MDVAVKKVEVRQLKKGKYIMIDDEPCKIVEYTTSSPGKHGSAKARIVAVGLFDGKKRTLTKPVDAKVDVPVIERKTAQVV
SDMGDTVQLMDMETYETFEVQKPEDEELASQLEPGTMVEYMEAAGKRKIVGIKEEE
>Mature_136_residues
MDVAVKKVEVRQLKKGKYIMIDDEPCKIVEYTTSSPGKHGSAKARIVAVGLFDGKKRTLTKPVDAKVDVPVIERKTAQVV
SDMGDTVQLMDMETYETFEVQKPEDEELASQLEPGTMVEYMEAAGKRKIVGIKEEE

Specific function: Functions by promoting the formation of the first peptide bond

COG id: COG0231

COG function: function code J; Translation elongation factor P (EF-P)/translation initiation factor 5A (eIF-5A)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eIF-5A family

Homologues:

Organism=Homo sapiens, GI9966867, Length=106, Percent_Identity=34.9056603773585, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17534327, Length=132, Percent_Identity=34.8484848484849, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71990399, Length=132, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320801, Length=136, Percent_Identity=32.3529411764706, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322507, Length=136, Percent_Identity=30.1470588235294, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922878, Length=132, Percent_Identity=29.5454545454545, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24762531, Length=132, Percent_Identity=29.5454545454545, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IF5A_METKA (Q8TXD5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614023.1
- ProteinModelPortal:   Q8TXD5
- SMR:   Q8TXD5
- GeneID:   1476840
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0739
- NMPDR:   fig|190192.1.peg.736
- HOGENOM:   HBG497107
- OMA:   IMRIKGE
- ProtClustDB:   PRK03999
- BioCyc:   MKAN190192:MK0739-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00085
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR019769
- InterPro:   IPR001884
- InterPro:   IPR022847
- InterPro:   IPR020189
- InterPro:   IPR013185
- InterPro:   IPR014722
- InterPro:   IPR008991
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:2.30.30.30
- PIRSF:   PIRSF003025
- TIGRFAMs:   TIGR00037

Pfam domain/function: PF08207 EFP_N; PF01287 eIF-5a; SSF50249 Nucleic_acid_OB; SSF50104 Transl_SH3_like

EC number: NA

Molecular weight: Translated: 15179; Mature: 15179

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00302 IF5A_HYPUSINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVAVKKVEVRQLKKGKYIMIDDEPCKIVEYTTSSPGKHGSAKARIVAVGLFDGKKRTLT
CCCCHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCC
KPVDAKVDVPVIERKTAQVVSDMGDTVQLMDMETYETFEVQKPEDEELASQLEPGTMVEY
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCHHHHHHHCCCCHHHHH
MEAAGKRKIVGIKEEE
HHHCCCEEEEEEECCC
>Mature Secondary Structure
MDVAVKKVEVRQLKKGKYIMIDDEPCKIVEYTTSSPGKHGSAKARIVAVGLFDGKKRTLT
CCCCHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCC
KPVDAKVDVPVIERKTAQVVSDMGDTVQLMDMETYETFEVQKPEDEELASQLEPGTMVEY
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCHHHHHHHCCCCHHHHH
MEAAGKRKIVGIKEEE
HHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014