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The map label for this gene is gspE [C]

Identifier: 20094144

GI number: 20094144

Start: 670334

End: 672142

Strand: Reverse

Name: gspE [C]

Synonym: MK0707

Alternate gene names: 20094144

Gene position: 672142-670334 (Counterclockwise)

Preceding gene: 20094145

Following gene: 20094143

Centisome position: 39.66

GC content: 49.47

Gene sequence:

>1809_bases
GTGGTGTGGACGGTGCCCCGGAAAAGGAACATCAAAGACCTGATCGGGGGCCTGGAGGAGGACGACCTCCTCCGCGACAT
ATTAGGGGAGAAGGAGGAAGAGTCGAAGTCGGAAAGCCCCGAGACCGTCGAGGAAACCGCCGAAGCCAGTGGAGAGCACT
ACGAGGCTGAGGAATCGAAGATCTCCCCACTGTCATCCGAGAAAGCCGCGGAGGTCCTGGAAGCGCACGAACTGGCGGAG
AGGGAGGGATGGGAAGGGAAGATACTGTGTGACGACAACATCCGCAGGATCGTGGAGTACCCGGATCAACCGGTGCCAGT
ATACATATCGAAAGAGTACGAGCAGTACTACAACTTCGAACGTATTAGGACACAAGTTCTAAAACGCCTGGGATCACAGG
CTCCACCGTACGCCCGTGATGACGAAGAGTTTCGGAAAGTTCTTGACGAGATTAAGGATATTCTATCGATGAATATCGAC
TTCGATCCGACGGAATACGCGACCCTTAAAGAGAGAGAAAAGGCGTTTGTCGAGCTAGTGAAGGAGAACTTCGACGCAGT
GTTAGAGGAGTACCCAGAACTTGAAGTTGTTAAGGATGATTTAGCCCATTTACTTATGTATGAAATGGTTGGGTACAAGG
AGATACATCCGCTGTTGAAAGACGACAATCTAGAGGAGATAATGGTAGTACCTGAGGTCGTACTCGGTGGTACTGCCAAG
CATTGCTTCATTTACGTATATGACCGTGATCATGGAATGTGTCTATGCAATTTCCGAGTCACTTCACGTTCAATTCGCAC
GGTGATCGAGCGGATTTCGCGAGAATCAGGTCGAAGAATAGATCAGGAAAACCCACTTCTCGATGCGCATTTACCGGATG
GCAGTCGAGTGAATGCCACTATCCCTCCGGTGAGTCCGGACGGTCCCACGCTTACAATTCGTAAGTTTAGAGAGGATCCA
CTGACGATAACTGACATAATCAAGTTTGGAACTATGAGTTATGAAGCTGCGGCATATTTGTGGCTAGCCGTTGAATATGG
GGCGAACATTCTAATTGCTGGAGGTACAGGTAGTGGTAAAACTACGACTTTAAACTGTCTCTGTATTTTCATCCCTCCGG
AGGAGAGAGTGATCACTATTGAGGACACTCTCGAGCTCCAACTTCCTCACGAACACTGGGTACGACTGACGACCAAACCA
CCTAACGTTGAAGGTCGTGGCGAAATTACTATGGATGATCTCGTAAAGAACACACTCCGACAGCGGCCTGATAGGATTAT
AGTGGGAGAGGTGCGAGGTCCCGAAGCACGTACATTGTTCACCGCTATGAATACGGGACATGACGGTTGTATGGGTACCT
TACACGCTAACACAGCTCGTGAAACTATCACCAGACTCACCAATGAACCCATGAACGTCCCAAAGATTATGATCCCAGCG
CTCGACATTATAGTGATGCAGAATAGGTTCCTCCAGCGTGGGTCCGGATCAATTCGACGTATCACGGAGATCTCGGAAAT
CGCCGGAATGGAGGGTGACACCGTCCAGCTGAACACGGTGTTCGAGTGGAAGCCTGAAACTGATGAGGTTACGAGTACCG
AAGTACCTAGCATGGTCTTCAAGAAGATTCAGGAGAAAACCGGAATGAGTATGGACGAAATACTCCATGAAATTGAGATA
CGTAAGCGAATCTTGAAGTACTTGGTCGACAATAATATCAGGCACGTGAGGGAGGTCGGGGAGTTCATACACGAGTTCTA
CAAGAACAGAGAGTCGGTGCTGGAGAAGGTGGGCATCTCGGGGTACTAA

Upstream 100 bases:

>100_bases
CTGGGAAAGAAGAGCGAGAGACAGAGATCATTGAGGAAGAAACGGAGGACATCGAAGACGTAGACGACCTACTTAAGGAC
CTGGAGCTATAACACCGAAG

Downstream 100 bases:

>100_bases
ACGTTGCTCCCGTCCCCTGAGACTCTACTCACGATCATCGGCGTGGGAGTCGGCGGCGCGGTAGTCCTCTACGTGACGGG
AGTGGCTGAACGCGTAGGTC

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MVWTVPRKRNIKDLIGGLEEDDLLRDILGEKEEESKSESPETVEETAEASGEHYEAEESKISPLSSEKAAEVLEAHELAE
REGWEGKILCDDNIRRIVEYPDQPVPVYISKEYEQYYNFERIRTQVLKRLGSQAPPYARDDEEFRKVLDEIKDILSMNID
FDPTEYATLKEREKAFVELVKENFDAVLEEYPELEVVKDDLAHLLMYEMVGYKEIHPLLKDDNLEEIMVVPEVVLGGTAK
HCFIYVYDRDHGMCLCNFRVTSRSIRTVIERISRESGRRIDQENPLLDAHLPDGSRVNATIPPVSPDGPTLTIRKFREDP
LTITDIIKFGTMSYEAAAYLWLAVEYGANILIAGGTGSGKTTTLNCLCIFIPPEERVITIEDTLELQLPHEHWVRLTTKP
PNVEGRGEITMDDLVKNTLRQRPDRIIVGEVRGPEARTLFTAMNTGHDGCMGTLHANTARETITRLTNEPMNVPKIMIPA
LDIIVMQNRFLQRGSGSIRRITEISEIAGMEGDTVQLNTVFEWKPETDEVTSTEVPSMVFKKIQEKTGMSMDEILHEIEI
RKRILKYLVDNNIRHVREVGEFIHEFYKNRESVLEKVGISGY

Sequences:

>Translated_602_residues
MVWTVPRKRNIKDLIGGLEEDDLLRDILGEKEEESKSESPETVEETAEASGEHYEAEESKISPLSSEKAAEVLEAHELAE
REGWEGKILCDDNIRRIVEYPDQPVPVYISKEYEQYYNFERIRTQVLKRLGSQAPPYARDDEEFRKVLDEIKDILSMNID
FDPTEYATLKEREKAFVELVKENFDAVLEEYPELEVVKDDLAHLLMYEMVGYKEIHPLLKDDNLEEIMVVPEVVLGGTAK
HCFIYVYDRDHGMCLCNFRVTSRSIRTVIERISRESGRRIDQENPLLDAHLPDGSRVNATIPPVSPDGPTLTIRKFREDP
LTITDIIKFGTMSYEAAAYLWLAVEYGANILIAGGTGSGKTTTLNCLCIFIPPEERVITIEDTLELQLPHEHWVRLTTKP
PNVEGRGEITMDDLVKNTLRQRPDRIIVGEVRGPEARTLFTAMNTGHDGCMGTLHANTARETITRLTNEPMNVPKIMIPA
LDIIVMQNRFLQRGSGSIRRITEISEIAGMEGDTVQLNTVFEWKPETDEVTSTEVPSMVFKKIQEKTGMSMDEILHEIEI
RKRILKYLVDNNIRHVREVGEFIHEFYKNRESVLEKVGISGY
>Mature_602_residues
MVWTVPRKRNIKDLIGGLEEDDLLRDILGEKEEESKSESPETVEETAEASGEHYEAEESKISPLSSEKAAEVLEAHELAE
REGWEGKILCDDNIRRIVEYPDQPVPVYISKEYEQYYNFERIRTQVLKRLGSQAPPYARDDEEFRKVLDEIKDILSMNID
FDPTEYATLKEREKAFVELVKENFDAVLEEYPELEVVKDDLAHLLMYEMVGYKEIHPLLKDDNLEEIMVVPEVVLGGTAK
HCFIYVYDRDHGMCLCNFRVTSRSIRTVIERISRESGRRIDQENPLLDAHLPDGSRVNATIPPVSPDGPTLTIRKFREDP
LTITDIIKFGTMSYEAAAYLWLAVEYGANILIAGGTGSGKTTTLNCLCIFIPPEERVITIEDTLELQLPHEHWVRLTTKP
PNVEGRGEITMDDLVKNTLRQRPDRIIVGEVRGPEARTLFTAMNTGHDGCMGTLHANTARETITRLTNEPMNVPKIMIPA
LDIIVMQNRFLQRGSGSIRRITEISEIAGMEGDTVQLNTVFEWKPETDEVTSTEVPSMVFKKIQEKTGMSMDEILHEIEI
RKRILKYLVDNNIRHVREVGEFIHEFYKNRESVLEKVGISGY

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=158, Percent_Identity=32.2784810126582, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786296, Length=178, Percent_Identity=27.5280898876405, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 68899; Mature: 68899

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWTVPRKRNIKDLIGGLEEDDLLRDILGEKEEESKSESPETVEETAEASGEHYEAEESK
CEECCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCCCCCCCHHH
ISPLSSEKAAEVLEAHELAEREGWEGKILCDDNIRRIVEYPDQPVPVYISKEYEQYYNFE
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCEEEEEECHHHHHHCHH
RIRTQVLKRLGSQAPPYARDDEEFRKVLDEIKDILSMNIDFDPTEYATLKEREKAFVELV
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KENFDAVLEEYPELEVVKDDLAHLLMYEMVGYKEIHPLLKDDNLEEIMVVPEVVLGGTAK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
HCFIYVYDRDHGMCLCNFRVTSRSIRTVIERISRESGRRIDQENPLLDAHLPDGSRVNAT
EEEEEEEECCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEE
IPPVSPDGPTLTIRKFREDPLTITDIIKFGTMSYEAAAYLWLAVEYGANILIAGGTGSGK
CCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHEEEEEECCCEEEEECCCCCCC
TTTLNCLCIFIPPEERVITIEDTLELQLPHEHWVRLTTKPPNVEGRGEITMDDLVKNTLR
EEEEEEEEEEECCCCCEEEEECCEEEECCHHHEEEEECCCCCCCCCCCEEHHHHHHHHHH
QRPDRIIVGEVRGPEARTLFTAMNTGHDGCMGTLHANTARETITRLTNEPMNVPKIMIPA
CCCCEEEEECCCCCCHHEEEEECCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCHHHHH
LDIIVMQNRFLQRGSGSIRRITEISEIAGMEGDTVQLNTVFEWKPETDEVTSTEVPSMVF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHH
KKIQEKTGMSMDEILHEIEIRKRILKYLVDNNIRHVREVGEFIHEFYKNRESVLEKVGIS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCC
GY
CC
>Mature Secondary Structure
MVWTVPRKRNIKDLIGGLEEDDLLRDILGEKEEESKSESPETVEETAEASGEHYEAEESK
CEECCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCCCCCCCHHH
ISPLSSEKAAEVLEAHELAEREGWEGKILCDDNIRRIVEYPDQPVPVYISKEYEQYYNFE
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCEEEEEECHHHHHHCHH
RIRTQVLKRLGSQAPPYARDDEEFRKVLDEIKDILSMNIDFDPTEYATLKEREKAFVELV
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KENFDAVLEEYPELEVVKDDLAHLLMYEMVGYKEIHPLLKDDNLEEIMVVPEVVLGGTAK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
HCFIYVYDRDHGMCLCNFRVTSRSIRTVIERISRESGRRIDQENPLLDAHLPDGSRVNAT
EEEEEEEECCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEE
IPPVSPDGPTLTIRKFREDPLTITDIIKFGTMSYEAAAYLWLAVEYGANILIAGGTGSGK
CCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHEEEEEECCCEEEEECCCCCCC
TTTLNCLCIFIPPEERVITIEDTLELQLPHEHWVRLTTKPPNVEGRGEITMDDLVKNTLR
EEEEEEEEEEECCCCCEEEEECCEEEECCHHHEEEEECCCCCCCCCCCEEHHHHHHHHHH
QRPDRIIVGEVRGPEARTLFTAMNTGHDGCMGTLHANTARETITRLTNEPMNVPKIMIPA
CCCCEEEEECCCCCCHHEEEEECCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCHHHHH
LDIIVMQNRFLQRGSGSIRRITEISEIAGMEGDTVQLNTVFEWKPETDEVTSTEVPSMVF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHH
KKIQEKTGMSMDEILHEIEIRKRILKYLVDNNIRHVREVGEFIHEFYKNRESVLEKVGIS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCC
GY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]