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The map label for this gene is spcS

Identifier: 20094110

GI number: 20094110

Start: 629019

End: 630314

Strand: Reverse

Name: spcS

Synonym: MK0672

Alternate gene names: 20094110

Gene position: 630314-629019 (Counterclockwise)

Preceding gene: 20094115

Following gene: 20094109

Centisome position: 37.19

GC content: 64.58

Gene sequence:

>1296_bases
ATGCGGGGACTCATCCCCGACCACATGCTGGAGCGTGGGCGTACCGTGCTCGACTCCTACAGGGAACCCGTGGAACGACT
CCTCAGCGAGCGTAGGATGCCGGAGGAGGGCTGGCCGGACGACGTCATCGCGACGTTCCTGTGGGAGCTGTCGAGGATGG
ACACCGACAAGGACCCCAAGGCGGCACGTATCGGGGAGAGGGAAGCCCGGGTAGCCTCAAGGCTTGCGGAGGAGTCCGTG
TTCGGGTTCTGCCACGGTGTGGGTAGGAGCGGGACGCTCGTGGATCCACAGCCGAAGGCTCCGGGCGCCTCGATAATGTA
CGCGTTAACGAACAGGCTGGTCACGGACTTCCTCCGGAGGCTCGGGTTCCGGATCGAAGGTGCCTTCGTGGTACCCGGTG
CTACGGGACTGTCGATAGCGCTGTGCCTGTCGGCGTTGGGTGAAGGCGAGGAGGTGATCTATCCGTACGCCGCCCACAAG
AGTCCGATTAAAGCCGTGCGACTGGCCGGCTTCGGGATGAGGGTGGTGGACACGGAGATCGAGGGTGACAGGATCGTGGT
GGACCCAGGGGATGTGGAGGAGGCCTTGGAGCGCTCGGAGTCCCCGGCTGCCGTGCTGAGCACGCTGACGTTCTTCCCAC
CGCGAAGCAGCGATCCGCTGCCCGAGATCGCCGAACTCTGCGAGGAGTACGGGGTCCCTCACGTCGTGAACGCCGCCTAC
GGGATCCAGCACGAGCAGTACCGAGATCTACTGAACCGAGCGATCAAGCGAGGGCGCGTGGACGTGGTCGTAAGCTCGAC
GGACAAGAACCTCCTAACACCCGTGGGTGGAGGGATCGTGTACGCGCCGGACGAGGAGACGCTCCGGGAGGTTTCCAGGG
CTTATCCTGGTCGCGCCAGCGCGGCACCGGTGGCCCACGCGTTAATCTCGCTGCTCTCCTTGGGGATGAAGGGATACCGG
CGCCTGATGCGGAGGCAGAAGGAGTGCAAAGCGCTTTTGGACGAGCTGTTGGAGGATCTCGAGGCCCGTCGAGACGACGT
CCGCGTGCTCGACGTGGATAACCCGATAGCCTCGGCGGTCGCCGTGGAAGGGCACGACCCCGTGGACCTCGCGGCCAGGC
TGTACGTGAGGAGGGTCACGGGACCGAGGGGTGTTCGTGCGGACGATCCGTTCGGAACCAGCCGGCTCCGGGGGTACCAT
TCGAACTACATCACCATCAACGCTGCCATCGGTGTCCGAGAGGAGGACGTCAAAACGGCCGTCGAAAGGCTCGAGCGGGA
GTTGGAGGGTGAGTGA

Upstream 100 bases:

>100_bases
CTCGAGCTCCTCGAGCGCCAGGAGTACCTCACGGGAGACGGCTCTCGACTTCCCACGTAGCTCGACGACCGGGGTGTCGA
CCTTGCTGGACCCGGAGGAC

Downstream 100 bases:

>100_bases
TTGCAGTTCAACCGAGTCAGGCTGGAGGGGGAGCGTGAGGAGCTGGAGATCAGAATAGGGAGCGCGAATGTGAAGCGGAA
GCTCGCCATGCTGATCCGGG

Product: pyridoxal-phosphate-dependent enzyme related to glutamate decarboxylase

Products: NA

Alternate protein names: Selenocysteine synthase; Sec synthase; Selenocysteinyl-tRNA(Sec) synthase; Sep-tRNA:Sec-tRNA synthase; SepSecS

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MRGLIPDHMLERGRTVLDSYREPVERLLSERRMPEEGWPDDVIATFLWELSRMDTDKDPKAARIGEREARVASRLAEESV
FGFCHGVGRSGTLVDPQPKAPGASIMYALTNRLVTDFLRRLGFRIEGAFVVPGATGLSIALCLSALGEGEEVIYPYAAHK
SPIKAVRLAGFGMRVVDTEIEGDRIVVDPGDVEEALERSESPAAVLSTLTFFPPRSSDPLPEIAELCEEYGVPHVVNAAY
GIQHEQYRDLLNRAIKRGRVDVVVSSTDKNLLTPVGGGIVYAPDEETLREVSRAYPGRASAAPVAHALISLLSLGMKGYR
RLMRRQKECKALLDELLEDLEARRDDVRVLDVDNPIASAVAVEGHDPVDLAARLYVRRVTGPRGVRADDPFGTSRLRGYH
SNYITINAAIGVREEDVKTAVERLERELEGE

Sequences:

>Translated_431_residues
MRGLIPDHMLERGRTVLDSYREPVERLLSERRMPEEGWPDDVIATFLWELSRMDTDKDPKAARIGEREARVASRLAEESV
FGFCHGVGRSGTLVDPQPKAPGASIMYALTNRLVTDFLRRLGFRIEGAFVVPGATGLSIALCLSALGEGEEVIYPYAAHK
SPIKAVRLAGFGMRVVDTEIEGDRIVVDPGDVEEALERSESPAAVLSTLTFFPPRSSDPLPEIAELCEEYGVPHVVNAAY
GIQHEQYRDLLNRAIKRGRVDVVVSSTDKNLLTPVGGGIVYAPDEETLREVSRAYPGRASAAPVAHALISLLSLGMKGYR
RLMRRQKECKALLDELLEDLEARRDDVRVLDVDNPIASAVAVEGHDPVDLAARLYVRRVTGPRGVRADDPFGTSRLRGYH
SNYITINAAIGVREEDVKTAVERLERELEGE
>Mature_431_residues
MRGLIPDHMLERGRTVLDSYREPVERLLSERRMPEEGWPDDVIATFLWELSRMDTDKDPKAARIGEREARVASRLAEESV
FGFCHGVGRSGTLVDPQPKAPGASIMYALTNRLVTDFLRRLGFRIEGAFVVPGATGLSIALCLSALGEGEEVIYPYAAHK
SPIKAVRLAGFGMRVVDTEIEGDRIVVDPGDVEEALERSESPAAVLSTLTFFPPRSSDPLPEIAELCEEYGVPHVVNAAY
GIQHEQYRDLLNRAIKRGRVDVVVSSTDKNLLTPVGGGIVYAPDEETLREVSRAYPGRASAAPVAHALISLLSLGMKGYR
RLMRRQKECKALLDELLEDLEARRDDVRVLDVDNPIASAVAVEGHDPVDLAARLYVRRVTGPRGVRADDPFGTSRLRGYH
SNYITINAAIGVREEDVKTAVERLERELEGE

Specific function: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl- tRNA(Sec) required for selenoprotein biosynthesis

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SepSecS family

Homologues:

Organism=Homo sapiens, GI267844904, Length=439, Percent_Identity=35.0797266514806, Blast_Score=264, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17559116, Length=438, Percent_Identity=30.1369863013699, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI212646307, Length=438, Percent_Identity=30.1369863013699, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI221377925, Length=437, Percent_Identity=32.0366132723112, Blast_Score=234, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPCS_METKA (Q8TXK0)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613957.1
- ProteinModelPortal:   Q8TXK0
- SMR:   Q8TXK0
- GeneID:   1476773
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0672
- NMPDR:   fig|190192.1.peg.670
- HOGENOM:   HBG716333
- OMA:   HKSPIKA
- ProtClustDB:   CLSK876261
- BioCyc:   MKAN190192:MK0672-MONOMER
- GO:   GO:0006412
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR019872
- InterPro:   IPR008829
- Gene3D:   G3DSA:3.40.640.10
- PANTHER:   PTHR12944
- TIGRFAMs:   TIGR03531

Pfam domain/function: PF05889 SLA_LP_auto_ag; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.9.1.2

Molecular weight: Translated: 47590; Mature: 47590

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLIPDHMLERGRTVLDSYREPVERLLSERRMPEEGWPDDVIATFLWELSRMDTDKDPK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
AARIGEREARVASRLAEESVFGFCHGVGRSGTLVDPQPKAPGASIMYALTNRLVTDFLRR
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGFRIEGAFVVPGATGLSIALCLSALGEGEEVIYPYAAHKSPIKAVRLAGFGMRVVDTEI
CCCEECCEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCEEEEEEEC
EGDRIVVDPGDVEEALERSESPAAVLSTLTFFPPRSSDPLPEIAELCEEYGVPHVVNAAY
CCCEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHH
GIQHEQYRDLLNRAIKRGRVDVVVSSTDKNLLTPVGGGIVYAPDEETLREVSRAYPGRAS
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCC
AAPVAHALISLLSLGMKGYRRLMRRQKECKALLDELLEDLEARRDDVRVLDVDNPIASAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHE
AVEGHDPVDLAARLYVRRVTGPRGVRADDPFGTSRLRGYHSNYITINAAIGVREEDVKTA
EECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEEEEECCCHHHHHHH
VERLERELEGE
HHHHHHHHCCC
>Mature Secondary Structure
MRGLIPDHMLERGRTVLDSYREPVERLLSERRMPEEGWPDDVIATFLWELSRMDTDKDPK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
AARIGEREARVASRLAEESVFGFCHGVGRSGTLVDPQPKAPGASIMYALTNRLVTDFLRR
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGFRIEGAFVVPGATGLSIALCLSALGEGEEVIYPYAAHKSPIKAVRLAGFGMRVVDTEI
CCCEECCEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCEEEEEEEC
EGDRIVVDPGDVEEALERSESPAAVLSTLTFFPPRSSDPLPEIAELCEEYGVPHVVNAAY
CCCEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHH
GIQHEQYRDLLNRAIKRGRVDVVVSSTDKNLLTPVGGGIVYAPDEETLREVSRAYPGRAS
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCC
AAPVAHALISLLSLGMKGYRRLMRRQKECKALLDELLEDLEARRDDVRVLDVDNPIASAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHE
AVEGHDPVDLAARLYVRRVTGPRGVRADDPFGTSRLRGYHSNYITINAAIGVREEDVKTA
EECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEEEEECCCHHHHHHH
VERLERELEGE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014