Click here to switch to the map view.

The map label for this gene is LasT [C]

Identifier: 20093957

GI number: 20093957

Start: 483150

End: 483854

Strand: Reverse

Name: LasT [C]

Synonym: MK0519

Alternate gene names: 20093957

Gene position: 483854-483150 (Counterclockwise)

Preceding gene: 20093959

Following gene: 20093956

Centisome position: 28.55

GC content: 55.32

Gene sequence:

>705_bases
TTGGACGTGCGGGTGGTACTGGTAGAACCGAAGTACGACGGGAACATCGGTCACGTAGCCCGTGTCATGAAAAACTTCGG
GGTAAAGGAGCTCTTTTTAGTAAGACCTAGGTCAGAGCTCGGTGAAGTGGCGATAGCTCGGGCGATGCACGCGCTCGACC
TGTTGGAGAACGCGGTGATAGTGAACACGTTGGAGGAGGCTATCGAGGACGTGGAGGCGGCCATAGCGACGACCGCCGTC
CTCGCCTCAACGCCCGCTAGGAACCCGATGATGCCCTGGGAGGTCAGAGAGGCGTTTTCGGACTACGAGAAGATCGCCGT
GGTATTCGGGCCCGAAGATAGAGGTCTGAGGACTTGGGAGGTCGAGCTTTGCGACGCCACCCTTCATATCCCGACAAGCG
AGGAGTACCGTTCCATGAACCTCTCGCATTCCGTGGCTGTAGTACTTTACGAGCTTAAGAAGATGGAGTACGTGCCGGAA
CGGTACGGTCGTGCTGCCACTAAGCGAGAGAAGGAGGTGCTAGTGAAGTCCTTGGACGACCTCATGAAGGCTTTGAACTT
CGACAGTACCCGTCGCGAGAACGTGTGCACGACCTTCCGGAGGTTTCTCGCCAGAGCAAGATGCACAGATAAGGAAATCA
AAGCACTACTGTGGATATTCGAGAAAGCTAAACGACGTGTTCTAGGGGTTAAGAGACGGAACTAG

Upstream 100 bases:

>100_bases
TTCGATCTCGACCTCCCCGTCCCAGTAGTTAGTCCTTCGCTCCCGAAACTCGTTCACGACCATACCCTCGAGCAAAGACT
GCACCCCGGAGGAATCCAGC

Downstream 100 bases:

>100_bases
CGGAATACGAATCTGTGAACGTTCACGTATCTGTACCCGAAGACTTTCACCCTGCGATAATATATTGCGAACTCAATACA
CCATATCGCCAGTAAGGCTA

Product: rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MDVRVVLVEPKYDGNIGHVARVMKNFGVKELFLVRPRSELGEVAIARAMHALDLLENAVIVNTLEEAIEDVEAAIATTAV
LASTPARNPMMPWEVREAFSDYEKIAVVFGPEDRGLRTWEVELCDATLHIPTSEEYRSMNLSHSVAVVLYELKKMEYVPE
RYGRAATKREKEVLVKSLDDLMKALNFDSTRRENVCTTFRRFLARARCTDKEIKALLWIFEKAKRRVLGVKRRN

Sequences:

>Translated_234_residues
MDVRVVLVEPKYDGNIGHVARVMKNFGVKELFLVRPRSELGEVAIARAMHALDLLENAVIVNTLEEAIEDVEAAIATTAV
LASTPARNPMMPWEVREAFSDYEKIAVVFGPEDRGLRTWEVELCDATLHIPTSEEYRSMNLSHSVAVVLYELKKMEYVPE
RYGRAATKREKEVLVKSLDDLMKALNFDSTRRENVCTTFRRFLARARCTDKEIKALLWIFEKAKRRVLGVKRRN
>Mature_234_residues
MDVRVVLVEPKYDGNIGHVARVMKNFGVKELFLVRPRSELGEVAIARAMHALDLLENAVIVNTLEEAIEDVEAAIATTAV
LASTPARNPMMPWEVREAFSDYEKIAVVFGPEDRGLRTWEVELCDATLHIPTSEEYRSMNLSHSVAVVLYELKKMEYVPE
RYGRAATKREKEVLVKSLDDLMKALNFDSTRRENVCTTFRRFLARARCTDKEIKALLWIFEKAKRRVLGVKRRN

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=248, Percent_Identity=31.8548387096774, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1790865, Length=180, Percent_Identity=30.5555555555556, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26811; Mature: 26811

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRVVLVEPKYDGNIGHVARVMKNFGVKELFLVRPRSELGEVAIARAMHALDLLENAVI
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VNTLEEAIEDVEAAIATTAVLASTPARNPMMPWEVREAFSDYEKIAVVFGPEDRGLRTWE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCEEEE
VELCDATLHIPTSEEYRSMNLSHSVAVVLYELKKMEYVPERYGRAATKREKEVLVKSLDD
EEEECEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LMKALNFDSTRRENVCTTFRRFLARARCTDKEIKALLWIFEKAKRRVLGVKRRN
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDVRVVLVEPKYDGNIGHVARVMKNFGVKELFLVRPRSELGEVAIARAMHALDLLENAVI
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VNTLEEAIEDVEAAIATTAVLASTPARNPMMPWEVREAFSDYEKIAVVFGPEDRGLRTWE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCEEEE
VELCDATLHIPTSEEYRSMNLSHSVAVVLYELKKMEYVPERYGRAATKREKEVLVKSLDD
EEEECEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LMKALNFDSTRRENVCTTFRRFLARARCTDKEIKALLWIFEKAKRRVLGVKRRN
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]