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The map label for this gene is EhaJ [C]

Identifier: 20093906

GI number: 20093906

Start: 432112

End: 432963

Strand: Reverse

Name: EhaJ [C]

Synonym: MK0468

Alternate gene names: 20093906

Gene position: 432963-432112 (Counterclockwise)

Preceding gene: 20093907

Following gene: 20093905

Centisome position: 25.54

GC content: 57.86

Gene sequence:

>852_bases
TTGATCGTGCTCGAAACGCTGGTGGGGATCACCGAAGCCTTCTTGGTCGGAAGCGTGTTCCTCGGACTGCATCGGAAGGT
AATGGCGCGCATTCAGCGTAGGCCGGGACCGCCGATAGTTCAGGAGTTCCTCCACACGTTGAAGTTTATGCTCAAGGAAG
GATACGCCCCACTGACCTCGGCGGAGTTCCTGTACTGGATGGTGCCGGTTTTCAACGTGATAATCTGGTCGGCTGCGGTC
ACCATCTCGGCAGTGTATCGGGGTAATTTGCTCCCGTTCTTCGCCCTGTACGCGTCCTACAAGGTTCTCTCCCACGGTGC
CGGAGTGTCCTCAGGTTCCACTTACACCAAGATCGGTGGTGTGCGACACGCCATTATGCCCGCCGGCGAGCTGGCGCTGG
CCTGTTCTCTGATATCGGCGTACTTCGTAACAGGGCACATGGATGTAACCGGCATACTGGCGTGGGAACACGCTCACGGC
CCGCTGATAGAACACATCCCGTTCTCGGCCTTGGCGTTCTTCACCCTCCTGTGGGTAGACTCACCTTACTCCCCGTTGGA
TCCTTCGAAGGGATACGACATCGTCGAGGGTTACCTCACGGAATATCCGGGGTTCCTGCGTGGGCTGATGTACGTGGCTG
AAGCCATCAAGTACTACTGTGAGCTCTGGGTCTTCCAGGCGGTGTTCTTGGGGATATCCGACCCGGTCTCTCACCTCCTG
ACCATGGGGGTCCTCACGCTACTCCTGGCCTCGATGTGCGCGCTGACGCCGATACTGAATCCGTACCAGGCCGTCGGGTT
CCACGTGATAATAGCCTCAATGATGTTCCTGGACTTCCTGGTCCTCGGATAG

Upstream 100 bases:

>100_bases
TCTAACGATCCTGGCGATCGCCCTCACGATCTTCGTCCTAGGATGGGCGCTCTCGCCTCGAGGTGATCGGGATAGGACAC
CTTTCCTGGGGTACTGAGCG

Downstream 100 bases:

>100_bases
GGGGGTCCCGTTGCGCTCGCACCTCTGCGTACTGTCGCTGTCCCTGGTCGGGATGGCCGCGATCCTGCTCCTCGAGCACT
CGATCTACGCTAAAGCCGGA

Product: membrane protein related to formate hydrogenlyase subunit 4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MIVLETLVGITEAFLVGSVFLGLHRKVMARIQRRPGPPIVQEFLHTLKFMLKEGYAPLTSAEFLYWMVPVFNVIIWSAAV
TISAVYRGNLLPFFALYASYKVLSHGAGVSSGSTYTKIGGVRHAIMPAGELALACSLISAYFVTGHMDVTGILAWEHAHG
PLIEHIPFSALAFFTLLWVDSPYSPLDPSKGYDIVEGYLTEYPGFLRGLMYVAEAIKYYCELWVFQAVFLGISDPVSHLL
TMGVLTLLLASMCALTPILNPYQAVGFHVIIASMMFLDFLVLG

Sequences:

>Translated_283_residues
MIVLETLVGITEAFLVGSVFLGLHRKVMARIQRRPGPPIVQEFLHTLKFMLKEGYAPLTSAEFLYWMVPVFNVIIWSAAV
TISAVYRGNLLPFFALYASYKVLSHGAGVSSGSTYTKIGGVRHAIMPAGELALACSLISAYFVTGHMDVTGILAWEHAHG
PLIEHIPFSALAFFTLLWVDSPYSPLDPSKGYDIVEGYLTEYPGFLRGLMYVAEAIKYYCELWVFQAVFLGISDPVSHLL
TMGVLTLLLASMCALTPILNPYQAVGFHVIIASMMFLDFLVLG
>Mature_283_residues
MIVLETLVGITEAFLVGSVFLGLHRKVMARIQRRPGPPIVQEFLHTLKFMLKEGYAPLTSAEFLYWMVPVFNVIIWSAAV
TISAVYRGNLLPFFALYASYKVLSHGAGVSSGSTYTKIGGVRHAIMPAGELALACSLISAYFVTGHMDVTGILAWEHAHG
PLIEHIPFSALAFFTLLWVDSPYSPLDPSKGYDIVEGYLTEYPGFLRGLMYVAEAIKYYCELWVFQAVFLGISDPVSHLL
TMGVLTLLLASMCALTPILNPYQAVGFHVIIASMMFLDFLVLG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: =1.6.5.3 [H]

Molecular weight: Translated: 31180; Mature: 31180

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLETLVGITEAFLVGSVFLGLHRKVMARIQRRPGPPIVQEFLHTLKFMLKEGYAPLTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCH
AEFLYWMVPVFNVIIWSAAVTISAVYRGNLLPFFALYASYKVLSHGAGVSSGSTYTKIGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
VRHAIMPAGELALACSLISAYFVTGHMDVTGILAWEHAHGPLIEHIPFSALAFFTLLWVD
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHHHCC
SPYSPLDPSKGYDIVEGYLTEYPGFLRGLMYVAEAIKYYCELWVFQAVFLGISDPVSHLL
CCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
TMGVLTLLLASMCALTPILNPYQAVGFHVIIASMMFLDFLVLG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIVLETLVGITEAFLVGSVFLGLHRKVMARIQRRPGPPIVQEFLHTLKFMLKEGYAPLTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCH
AEFLYWMVPVFNVIIWSAAVTISAVYRGNLLPFFALYASYKVLSHGAGVSSGSTYTKIGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
VRHAIMPAGELALACSLISAYFVTGHMDVTGILAWEHAHGPLIEHIPFSALAFFTLLWVD
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHHHCC
SPYSPLDPSKGYDIVEGYLTEYPGFLRGLMYVAEAIKYYCELWVFQAVFLGISDPVSHLL
CCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
TMGVLTLLLASMCALTPILNPYQAVGFHVIIASMMFLDFLVLG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]