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The map label for this gene is sepS

Identifier: 20093877

GI number: 20093877

Start: 409925

End: 411559

Strand: Reverse

Name: sepS

Synonym: MK0439

Alternate gene names: 20093877

Gene position: 411559-409925 (Counterclockwise)

Preceding gene: 20093881

Following gene: 20093876

Centisome position: 24.28

GC content: 63.0

Gene sequence:

>1635_bases
ATGCCGTTCGACCGTGACAAGCTGGAGGAACTGAGGTCCCTGGCCCAGCGCGATTTCGATCGAGCCTGGAAAGAGGGTGC
TAAGCTGGTCCGGGAGCCCGGCCTCCGGGACCGGTACCCGAGGCTTAAGGTGGAGACGGGCGAGCCCCATCCGCTGTTCG
AAACTATTCAGCAGCTGAGGGAGGCGTACCTGCGTGCGGGCTTCAGAGAGGTCGTGAACCCCGTCATCATCCCGGAGGAG
GAGGTGTACAAGCAGTTCGGTCCGGAGGCGGCGGCCGTGCTGGACAGGTGCTTCTACCTCGCCGGACTCCCGCGTCCGGA
CGTAGGATTGGGTGCGGACAAGGTCGAGAAGTTGGCGGAGGTGCTGGGTCGGGAGCCATCCGAAGACGAGGTGGAACGAC
TGAGGGAAACCCTGCACGCGTACAAGAAGGGCGAGATCGACGGTGACGAGCTGACACACGAGATCGCGGAGGCCCTGGAC
ACGGACGACGGGACGGCGGTGAGGATACTGGACGAGGTCTTCCCCGAGCTCAAGCGCCTGAAGCCCGAGCCGCTGGAGCC
TCCGCTCACCCTCCGATCGCACATGACCGCGGGTTGGTTTATAACGCTATCCGAGATACTGAAGCGTGAGGATCCGCCGT
TGAAATTGTTCTCCATCGACAGGTGCTTCCGACGCGAGCAGCGGGAGGACGAGAGCCACTTGATGACGTACCACTCCGCG
TCGTGCGTCGTGGTCAGCGACGACGTGACCGTGGACACCGGGAAGGCAGTAGCGGAGGCGATCCTCCGGCAGTTCGGGTT
CGAGGATTTCGAGTTCGTTCCCGATGAGAAAATGTCCAAATACTACGTACCCGGAACACAGACCGAGGTGTACGCTTACC
ACCCCGACCTGGAGGACTCCATCGAGGACGAGGAGCTGGGACCGGGTTGGGTGGAGATAGCGACGTTCGGGCTGTACTCA
CCGGTGGCGCTGGCGGAGTACGGGATTGACTACCCGGTCATGAACCTCGGGATCGGCGTGGAGCGGTTGTGCATGGTCCT
CCACGGTATCGACGACGTGCGTTCCCTCGCGTACGTCGAGTACGAACCGTGGGAACCTTCAGATCTGGAGCTGGCCAGGA
TGATCGACTACGAGCGGAAGCCGGCTACGTCGTTCGGGGAACGGCTGGTTCGTGAGGTGGTGCGGGGGCTTCACGAGCAC
GCCGACGAGGAGGGGCCCGTGGAAGTCGAGCTGTTCCGCGGGGAGTTTGGCGATCGGGAGGTGGTAGTCCACGCCGTCGA
GGAAGAGAAGGGAGAACCTCTCGCGGGGCCCGCGGCCTTCAACCGCGTTTACGTCCTCGATGGTAACCTCTACGCCGTCC
CGCCCGAGGGCGACTTCGGCCGTGAGATCCGCGAGGAGGGCGTGTACTCTGGGGTGTCATTCGAAGAAGGACTGGCGGCG
CGGTTGGCCTACGAGGTGGAGGAGCTGCTCGCGACTGGAGGAGGGGAGACCACCGTGTCGGTCCGTAAGGTGAGTCGCCC
CTCGCAGGTCAACCTGAGTCTTCCTCGGAAGCTGTTACGCTACGTAACCAAAAAAGGAGGTGAGATCGAAATCAAGGGGC
CGGTGTTCGTGACCCTCAGAGCCGAGGTCAGGTGA

Upstream 100 bases:

>100_bases
TCGGAAACCGCGTCCATATTCTTTCCGGTTCCGTCACGTTAACACCTCCGGGAAAAACCATGACCCGGCCCGCAGACCCG
GCATGTGAGGGGCACTGCGG

Downstream 100 bases:

>100_bases
CTGGGTCCCTCATCATCACATTTCCGCAATCCGCCGTACTCATCATCCGGGGGTGCATGCATCGGTGGGAAGTCCTTGGC
GCTACATCGTGACGGTAGCC

Product: O-phosphoseryl-tRNA synthetase

Products: NA

Alternate protein names: SepRS; Non-canonical O-phosphoseryl-tRNA(Cys) synthetase; O-phosphoserine--tRNA(Cys) ligase; O-phosphoserine--tRNA ligase

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MPFDRDKLEELRSLAQRDFDRAWKEGAKLVREPGLRDRYPRLKVETGEPHPLFETIQQLREAYLRAGFREVVNPVIIPEE
EVYKQFGPEAAAVLDRCFYLAGLPRPDVGLGADKVEKLAEVLGREPSEDEVERLRETLHAYKKGEIDGDELTHEIAEALD
TDDGTAVRILDEVFPELKRLKPEPLEPPLTLRSHMTAGWFITLSEILKREDPPLKLFSIDRCFRREQREDESHLMTYHSA
SCVVVSDDVTVDTGKAVAEAILRQFGFEDFEFVPDEKMSKYYVPGTQTEVYAYHPDLEDSIEDEELGPGWVEIATFGLYS
PVALAEYGIDYPVMNLGIGVERLCMVLHGIDDVRSLAYVEYEPWEPSDLELARMIDYERKPATSFGERLVREVVRGLHEH
ADEEGPVEVELFRGEFGDREVVVHAVEEEKGEPLAGPAAFNRVYVLDGNLYAVPPEGDFGREIREEGVYSGVSFEEGLAA
RLAYEVEELLATGGGETTVSVRKVSRPSQVNLSLPRKLLRYVTKKGGEIEIKGPVFVTLRAEVR

Sequences:

>Translated_544_residues
MPFDRDKLEELRSLAQRDFDRAWKEGAKLVREPGLRDRYPRLKVETGEPHPLFETIQQLREAYLRAGFREVVNPVIIPEE
EVYKQFGPEAAAVLDRCFYLAGLPRPDVGLGADKVEKLAEVLGREPSEDEVERLRETLHAYKKGEIDGDELTHEIAEALD
TDDGTAVRILDEVFPELKRLKPEPLEPPLTLRSHMTAGWFITLSEILKREDPPLKLFSIDRCFRREQREDESHLMTYHSA
SCVVVSDDVTVDTGKAVAEAILRQFGFEDFEFVPDEKMSKYYVPGTQTEVYAYHPDLEDSIEDEELGPGWVEIATFGLYS
PVALAEYGIDYPVMNLGIGVERLCMVLHGIDDVRSLAYVEYEPWEPSDLELARMIDYERKPATSFGERLVREVVRGLHEH
ADEEGPVEVELFRGEFGDREVVVHAVEEEKGEPLAGPAAFNRVYVLDGNLYAVPPEGDFGREIREEGVYSGVSFEEGLAA
RLAYEVEELLATGGGETTVSVRKVSRPSQVNLSLPRKLLRYVTKKGGEIEIKGPVFVTLRAEVR
>Mature_543_residues
PFDRDKLEELRSLAQRDFDRAWKEGAKLVREPGLRDRYPRLKVETGEPHPLFETIQQLREAYLRAGFREVVNPVIIPEEE
VYKQFGPEAAAVLDRCFYLAGLPRPDVGLGADKVEKLAEVLGREPSEDEVERLRETLHAYKKGEIDGDELTHEIAEALDT
DDGTAVRILDEVFPELKRLKPEPLEPPLTLRSHMTAGWFITLSEILKREDPPLKLFSIDRCFRREQREDESHLMTYHSAS
CVVVSDDVTVDTGKAVAEAILRQFGFEDFEFVPDEKMSKYYVPGTQTEVYAYHPDLEDSIEDEELGPGWVEIATFGLYSP
VALAEYGIDYPVMNLGIGVERLCMVLHGIDDVRSLAYVEYEPWEPSDLELARMIDYERKPATSFGERLVREVVRGLHEHA
DEEGPVEVELFRGEFGDREVVVHAVEEEKGEPLAGPAAFNRVYVLDGNLYAVPPEGDFGREIREEGVYSGVSFEEGLAAR
LAYEVEELLATGGGETTVSVRKVSRPSQVNLSLPRKLLRYVTKKGGEIEIKGPVFVTLRAEVR

Specific function: Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys)

COG id: COG2024

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit (archaeal type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=171, Percent_Identity=29.2397660818713, Blast_Score=72, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508957, Length=170, Percent_Identity=30, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI32563657, Length=170, Percent_Identity=30, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6321087, Length=195, Percent_Identity=29.7435897435897, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SEPS_METKA (Q8TY66)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613724.1
- ProteinModelPortal:   Q8TY66
- SMR:   Q8TY66
- GeneID:   1477742
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0439
- NMPDR:   fig|190192.1.peg.437
- HOGENOM:   HBG539012
- OMA:   TLRSHMT
- ProtClustDB:   PRK06253
- BioCyc:   MKAN190192:MK0439-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01674
- InterPro:   IPR006195
- InterPro:   IPR005246
- InterPro:   IPR002319
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00470

Pfam domain/function: PF01409 tRNA-synt_2d

EC number: =6.1.1.27

Molecular weight: Translated: 61614; Mature: 61483

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFDRDKLEELRSLAQRDFDRAWKEGAKLVREPGLRDRYPRLKVETGEPHPLFETIQQLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHH
EAYLRAGFREVVNPVIIPEEEVYKQFGPEAAAVLDRCFYLAGLPRPDVGLGADKVEKLAE
HHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
VLGREPSEDEVERLRETLHAYKKGEIDGDELTHEIAEALDTDDGTAVRILDEVFPELKRL
HHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
KPEPLEPPLTLRSHMTAGWFITLSEILKREDPPLKLFSIDRCFRREQREDESHLMTYHSA
CCCCCCCCHHHHHCCCCHHHEEHHHHHCCCCCCEEEEHHHHHHHHHHCCCHHHEEEEECC
SCVVVSDDVTVDTGKAVAEAILRQFGFEDFEFVPDEKMSKYYVPGTQTEVYAYHPDLEDS
EEEEEECCCEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCC
IEDEELGPGWVEIATFGLYSPVALAEYGIDYPVMNLGIGVERLCMVLHGIDDVRSLAYVE
CCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHCHHHHHHHEEEE
YEPWEPSDLELARMIDYERKPATSFGERLVREVVRGLHEHADEEGPVEVELFRGEFGDRE
ECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCE
VVVHAVEEEKGEPLAGPAAFNRVYVLDGNLYAVPPEGDFGREIREEGVYSGVSFEEGLAA
EEEEEEHHCCCCCCCCHHHCCEEEEEECCEEEECCCCCHHHHHHHCCCCCCCCHHHHHHH
RLAYEVEELLATGGGETTVSVRKVSRPSQVNLSLPRKLLRYVTKKGGEIEIKGPVFVTLR
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEE
AEVR
ECCC
>Mature Secondary Structure 
PFDRDKLEELRSLAQRDFDRAWKEGAKLVREPGLRDRYPRLKVETGEPHPLFETIQQLR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHH
EAYLRAGFREVVNPVIIPEEEVYKQFGPEAAAVLDRCFYLAGLPRPDVGLGADKVEKLAE
HHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
VLGREPSEDEVERLRETLHAYKKGEIDGDELTHEIAEALDTDDGTAVRILDEVFPELKRL
HHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
KPEPLEPPLTLRSHMTAGWFITLSEILKREDPPLKLFSIDRCFRREQREDESHLMTYHSA
CCCCCCCCHHHHHCCCCHHHEEHHHHHCCCCCCEEEEHHHHHHHHHHCCCHHHEEEEECC
SCVVVSDDVTVDTGKAVAEAILRQFGFEDFEFVPDEKMSKYYVPGTQTEVYAYHPDLEDS
EEEEEECCCEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCC
IEDEELGPGWVEIATFGLYSPVALAEYGIDYPVMNLGIGVERLCMVLHGIDDVRSLAYVE
CCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHCHHHHHHHEEEE
YEPWEPSDLELARMIDYERKPATSFGERLVREVVRGLHEHADEEGPVEVELFRGEFGDRE
ECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCE
VVVHAVEEEKGEPLAGPAAFNRVYVLDGNLYAVPPEGDFGREIREEGVYSGVSFEEGLAA
EEEEEEHHCCCCCCCCHHHCCEEEEEECCEEEECCCCCHHHHHHHCCCCCCCCHHHHHHH
RLAYEVEELLATGGGETTVSVRKVSRPSQVNLSLPRKLLRYVTKKGGEIEIKGPVFVTLR
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEE
AEVR
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014