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The map label for this gene is rgy [H]

Identifier: 20093729

GI number: 20093729

Start: 278976

End: 280052

Strand: Reverse

Name: rgy [H]

Synonym: MK0289

Alternate gene names: 20093729

Gene position: 280052-278976 (Counterclockwise)

Preceding gene: 20093731

Following gene: 20093728

Centisome position: 16.52

GC content: 59.61

Gene sequence:

>1077_bases
TTGAATGCGACACTCCGGATCCGGAACAGACCTGTCGCCGAGTCGACCTACACTTCGCTGAGGGGAGCGAAGGCTGAGGT
CGTCCGAGTTGAGCGAGAGGAGCGTGAAATACACCCTAAACCACCTTTCGAAACCGGAACCATGCTTCAGGCGGCTACCC
GACGACTTCGACTGTCGTCCGAGCGTGTCATGCAGCTGGCCCAGGACCTGTTCGAGGGAGGTCTCATCACATACCACCGG
ACCGACTCTACCAGGGTCTCGGAGGAGGGCAAGCGCGTAGCCCGGGACTACATCCGGGCTAACTTCGACCCCGAAGACTA
CAATCCGAGGACCTGGGAACCCGAGGCCGAGCACGTCGAAGGTGCTCACGAGTGTATAAGACCCACCAGACCGGCCGACG
CCGAGGAGCTACGGACGATGGTTCGTGAGGGAGCGATTCAGACCACCGTCACGCTGACTTCTCACCATTTACGTCTTTAC
GATCTCGTCTTCCGACGTTTCGTAGCCAGCCAGATGAAGCCCGCGAAGGTCCTCTACCAGGAGGCCGTGCTGGAGGTCGA
AGTCAAAGGAGTGCCCGTCGCGGAGCTCGAACTCAGCGGAGTTTTGGAGATCGTCGAGCCGGGCTTCACTAAGGTCCTAA
CGGAGTACGACCTGCCGGCGTACGGCATCCGGGAGACCCCGGAACTGGAGGAGGGAGATCGGCTCGAAATAGGCGACGTG
GAGGTGCTGGAACGGCATGAAGAGTACCCGTACGATCAATCGGAGTTGGTTGAAGACATGCGAGAGCGGGGACTCGGCCG
TCCTAGCACCTACGCGCAGATAGTCGAGAAGCTGTTCCGGCGTGGGTACGTGTACGAGGTACCGCAACGGAGGTGGATAT
TCCCGACGACCCGTGGTGAGGCCGTCTACGAGTACCTCAGTACCCACTACGAGCGGTTCGTATCGGAGGAAACTACGAGA
GACCTGGAGGAACGCATGGACGCCGTAGCGCTCGGTAAGGCCGAGTATCAAGAGGAGATGGAAAAACTCTACCTAGAGCT
CGAAAGGGTCGTCGAGATGCCTGACCCGGAACCGTGA

Upstream 100 bases:

>100_bases
AACGTGTCCATGCCCTATCCACCCGACCCGGGCCCGTTCTCCCGAAAGTCCATATTCACCTCGGCGCTCGCCCTAACCCT
GCGAGACCGGGGGATCGTAA

Downstream 100 bases:

>100_bases
TCGCCGATCACGTGCGGAGGTGAGCCGCCATAAGACGCAAAGTATACTTCTTTTCGGAGACGGGAAACACGCGACTGATC
GCCCAAGTGATCGCTTCGAC

Product: Reverse gyrase

Products: NA

Alternate protein names: Helicase; Topoisomerase [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MNATLRIRNRPVAESTYTSLRGAKAEVVRVEREEREIHPKPPFETGTMLQAATRRLRLSSERVMQLAQDLFEGGLITYHR
TDSTRVSEEGKRVARDYIRANFDPEDYNPRTWEPEAEHVEGAHECIRPTRPADAEELRTMVREGAIQTTVTLTSHHLRLY
DLVFRRFVASQMKPAKVLYQEAVLEVEVKGVPVAELELSGVLEIVEPGFTKVLTEYDLPAYGIRETPELEEGDRLEIGDV
EVLERHEEYPYDQSELVEDMRERGLGRPSTYAQIVEKLFRRGYVYEVPQRRWIFPTTRGEAVYEYLSTHYERFVSEETTR
DLEERMDAVALGKAEYQEEMEKLYLELERVVEMPDPEP

Sequences:

>Translated_358_residues
MNATLRIRNRPVAESTYTSLRGAKAEVVRVEREEREIHPKPPFETGTMLQAATRRLRLSSERVMQLAQDLFEGGLITYHR
TDSTRVSEEGKRVARDYIRANFDPEDYNPRTWEPEAEHVEGAHECIRPTRPADAEELRTMVREGAIQTTVTLTSHHLRLY
DLVFRRFVASQMKPAKVLYQEAVLEVEVKGVPVAELELSGVLEIVEPGFTKVLTEYDLPAYGIRETPELEEGDRLEIGDV
EVLERHEEYPYDQSELVEDMRERGLGRPSTYAQIVEKLFRRGYVYEVPQRRWIFPTTRGEAVYEYLSTHYERFVSEETTR
DLEERMDAVALGKAEYQEEMEKLYLELERVVEMPDPEP
>Mature_358_residues
MNATLRIRNRPVAESTYTSLRGAKAEVVRVEREEREIHPKPPFETGTMLQAATRRLRLSSERVMQLAQDLFEGGLITYHR
TDSTRVSEEGKRVARDYIRANFDPEDYNPRTWEPEAEHVEGAHECIRPTRPADAEELRTMVREGAIQTTVTLTSHHLRLY
DLVFRRFVASQMKPAKVLYQEAVLEVEVKGVPVAELELSGVLEIVEPGFTKVLTEYDLPAYGIRETPELEEGDRLEIGDV
EVLERHEEYPYDQSELVEDMRERGLGRPSTYAQIVEKLFRRGYVYEVPQRRWIFPTTRGEAVYEYLSTHYERFVSEETTR
DLEERMDAVALGKAEYQEEMEKLYLELERVVEMPDPEP

Specific function: Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process. It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue. May be involved in rewinding the DN

COG id: COG1110

COG function: function code L; Reverse gyrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=357, Percent_Identity=24.0896358543417, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1787529, Length=346, Percent_Identity=32.6589595375723, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=342, Percent_Identity=23.9766081871345, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24585251, Length=278, Percent_Identity=24.1007194244604, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR014021
- InterPro:   IPR005736
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00270 DEAD; PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =3.6.4.12; =5.99.1.3 [H]

Molecular weight: Translated: 41700; Mature: 41700

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATLRIRNRPVAESTYTSLRGAKAEVVRVEREEREIHPKPPFETGTMLQAATRRLRLSS
CCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCH
ERVMQLAQDLFEGGLITYHRTDSTRVSEEGKRVARDYIRANFDPEDYNPRTWEPEAEHVE
HHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
GAHECIRPTRPADAEELRTMVREGAIQTTVTLTSHHLRLYDLVFRRFVASQMKPAKVLYQ
HHHHHHCCCCCCCHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
EAVLEVEVKGVPVAELELSGVLEIVEPGFTKVLTEYDLPAYGIRETPELEEGDRLEIGDV
HHHHEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCH
EVLERHEEYPYDQSELVEDMRERGLGRPSTYAQIVEKLFRRGYVYEVPQRRWIFPTTRGE
HHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCCEECCCCCHH
AVYEYLSTHYERFVSEETTRDLEERMDAVALGKAEYQEEMEKLYLELERVVEMPDPEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNATLRIRNRPVAESTYTSLRGAKAEVVRVEREEREIHPKPPFETGTMLQAATRRLRLSS
CCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCH
ERVMQLAQDLFEGGLITYHRTDSTRVSEEGKRVARDYIRANFDPEDYNPRTWEPEAEHVE
HHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
GAHECIRPTRPADAEELRTMVREGAIQTTVTLTSHHLRLYDLVFRRFVASQMKPAKVLYQ
HHHHHHCCCCCCCHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
EAVLEVEVKGVPVAELELSGVLEIVEPGFTKVLTEYDLPAYGIRETPELEEGDRLEIGDV
HHHHEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCH
EVLERHEEYPYDQSELVEDMRERGLGRPSTYAQIVEKLFRRGYVYEVPQRRWIFPTTRGE
HHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCCEECCCCCHH
AVYEYLSTHYERFVSEETTRDLEERMDAVALGKAEYQEEMEKLYLELERVVEMPDPEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]