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The map label for this gene is HemL

Identifier: 20093720

GI number: 20093720

Start: 271207

End: 272499

Strand: Reverse

Name: HemL

Synonym: MK0280

Alternate gene names: 20093720

Gene position: 272499-271207 (Counterclockwise)

Preceding gene: 20093721

Following gene: 20093716

Centisome position: 16.08

GC content: 63.73

Gene sequence:

>1293_bases
TTGGGTTACGAGGACGAGTTCCCCGAGTCGCTCGAGCTCTTCAAGCGCGCCGAGCGAGTGATGCCGGGCGGCGTGAGCAG
TCCGGTCCGACGGTTCGATCCGTACCCGTTCTACGTCGAAAGGGCCGAGGGTTCGCGCCTGTACACCGTGGACGGACACG
TCCTGATTGACTACTGCTTGGCCTTCGGTCCGTTAATACTGGGACACGCCCACCCCGAGGTCGTAGAGGCCGTGGTCGAG
CGGGTGCGGGAGGGCTTCCACTACGGTACACCGACGCTCCCCGAGCTGAAGCTCGCGGAGAAAGTCGTCGAGCTGGTTCC
GAACGTGGAGAAGGTGAGATTGGTGAACACCGGCACCGAGGCGACGATGAGCGCGATACGGCTCGCCCGCGCGTACACGG
GCCGTGAGAAGATCGTGAAGTTCGAAGGGTGCTACCACGGAGCCCACGACGCGGTGCTCGTCCGGGCGGGATCCGGGGCC
TCCGAGCTCGGGGCGCCCGACTCACCGGGTATCCCGGAGTCGGTGGCGGAGAACACCCTCGTCTGCCCGTTCAACGACGT
CGAAGCTTTCGTGGAGACCGTGGAGAGGTTCGACGAGGAGATCGGTGCGGTGATCGTGGAACCCGTGCTGGGTAACGCCG
GCTGTGTGCCCCCCGACGAGGAGTTCCTGAAAGTACTTCGCGAGTACTGCGACGGGACCGAGCGGTTGCTGATCTTCGAC
GAGGTGATCACGGGATTCCGACTGGAGTTGGGTGGTGCCCAGGAGTACTACGGTATCGACGCGGACCTCGTCTGCCTGGG
TAAGATCCTGGGAGGAGGGCTCCCGATAGGGGCCTTCGGAGGCCCGGAGGAGTACATGTCGCGCGTTGCACCCGAGGGCA
AAGTATACCAGGCCGGCACGTTCAACGGCAACCCGGTGTCGGCTACGGCGGGCCTGGTGACGCTCGAGGTACTCGAACGT
GAACGACCCTACGACGAACTCTCATCGAAGGCGGAGCGGTTGGCCTCCGCCCTGGAGGACGGCCTCGAGGACCGTGGCAT
CGAAGGAGTCGTGAACCGAGTGGAGTCCATGTTCCAGGTGTACTTCGGGATCGAAGAGGTCCGAGATTACGCCGACGTAA
ACTCGGCCGACCACGATGCGTTCAAGCGGTTCCACAGGGAGTTGTTAGAGCACGGCGTATGGATCGCGGCCTCGAATTAC
GAGGCCTGGTTCCTGTCCATCGCGCACACCGAGACGGACCTGGAACGCACCGAGGAAGCCTTCGAAGAGGCACTCGATCG
GCTCACGGGATGA

Upstream 100 bases:

>100_bases
AAATTCGAAACGAATGGCGTCCCACGGAACTCCCTCCATACCGTAGCAAGTCGCGAGGCACTCATTAAGCACCTGCTCCT
ATGCCCCATCGGGGCTCAGC

Downstream 100 bases:

>100_bases
GGAGGCCGTACCGGGGGAGTCCCTGATGACCTGTACCTGGGCTTCCCCGACCGTTCCACCCCGAACGAACTCGAGGGGAT
GAGGAGGGAGGTTTCGTCGG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGYEDEFPESLELFKRAERVMPGGVSSPVRRFDPYPFYVERAEGSRLYTVDGHVLIDYCLAFGPLILGHAHPEVVEAVVE
RVREGFHYGTPTLPELKLAEKVVELVPNVEKVRLVNTGTEATMSAIRLARAYTGREKIVKFEGCYHGAHDAVLVRAGSGA
SELGAPDSPGIPESVAENTLVCPFNDVEAFVETVERFDEEIGAVIVEPVLGNAGCVPPDEEFLKVLREYCDGTERLLIFD
EVITGFRLELGGAQEYYGIDADLVCLGKILGGGLPIGAFGGPEEYMSRVAPEGKVYQAGTFNGNPVSATAGLVTLEVLER
ERPYDELSSKAERLASALEDGLEDRGIEGVVNRVESMFQVYFGIEEVRDYADVNSADHDAFKRFHRELLEHGVWIAASNY
EAWFLSIAHTETDLERTEEAFEEALDRLTG

Sequences:

>Translated_430_residues
MGYEDEFPESLELFKRAERVMPGGVSSPVRRFDPYPFYVERAEGSRLYTVDGHVLIDYCLAFGPLILGHAHPEVVEAVVE
RVREGFHYGTPTLPELKLAEKVVELVPNVEKVRLVNTGTEATMSAIRLARAYTGREKIVKFEGCYHGAHDAVLVRAGSGA
SELGAPDSPGIPESVAENTLVCPFNDVEAFVETVERFDEEIGAVIVEPVLGNAGCVPPDEEFLKVLREYCDGTERLLIFD
EVITGFRLELGGAQEYYGIDADLVCLGKILGGGLPIGAFGGPEEYMSRVAPEGKVYQAGTFNGNPVSATAGLVTLEVLER
ERPYDELSSKAERLASALEDGLEDRGIEGVVNRVESMFQVYFGIEEVRDYADVNSADHDAFKRFHRELLEHGVWIAASNY
EAWFLSIAHTETDLERTEEAFEEALDRLTG
>Mature_429_residues
GYEDEFPESLELFKRAERVMPGGVSSPVRRFDPYPFYVERAEGSRLYTVDGHVLIDYCLAFGPLILGHAHPEVVEAVVER
VREGFHYGTPTLPELKLAEKVVELVPNVEKVRLVNTGTEATMSAIRLARAYTGREKIVKFEGCYHGAHDAVLVRAGSGAS
ELGAPDSPGIPESVAENTLVCPFNDVEAFVETVERFDEEIGAVIVEPVLGNAGCVPPDEEFLKVLREYCDGTERLLIFDE
VITGFRLELGGAQEYYGIDADLVCLGKILGGGLPIGAFGGPEEYMSRVAPEGKVYQAGTFNGNPVSATAGLVTLEVLERE
RPYDELSSKAERLASALEDGLEDRGIEGVVNRVESMFQVYFGIEEVRDYADVNSADHDAFKRFHRELLEHGVWIAASNYE
AWFLSIAHTETDLERTEEAFEEALDRLTG

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=329, Percent_Identity=29.483282674772, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI24119277, Length=375, Percent_Identity=27.2, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI37574042, Length=417, Percent_Identity=23.7410071942446, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI13994255, Length=431, Percent_Identity=24.1299303944316, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI284507298, Length=239, Percent_Identity=31.3807531380753, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI226442709, Length=383, Percent_Identity=24.0208877284595, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI226442705, Length=333, Percent_Identity=24.3243243243243, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=48.8207547169811, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1789016, Length=348, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1788044, Length=297, Percent_Identity=33.6700336700337, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1789759, Length=317, Percent_Identity=29.3375394321767, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787560, Length=339, Percent_Identity=28.9085545722714, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI145693181, Length=319, Percent_Identity=31.6614420062696, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786991, Length=396, Percent_Identity=27.7777777777778, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25144271, Length=355, Percent_Identity=27.3239436619718, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32564660, Length=329, Percent_Identity=27.355623100304, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144274, Length=195, Percent_Identity=27.6923076923077, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323470, Length=351, Percent_Identity=26.7806267806268, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=326, Percent_Identity=26.9938650306748, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357415, Length=306, Percent_Identity=28.7581699346405, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161085790, Length=328, Percent_Identity=25.609756097561, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574759, Length=328, Percent_Identity=25.609756097561, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_METKA (Q8TYL6)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613567.1
- ProteinModelPortal:   Q8TYL6
- SMR:   Q8TYL6
- GeneID:   1477583
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0280
- NMPDR:   fig|190192.1.peg.280
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- BioCyc:   MKAN190192:MK0280-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 47422; Mature: 47291

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYEDEFPESLELFKRAERVMPGGVSSPVRRFDPYPFYVERAEGSRLYTVDGHVLIDYCL
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEECCCCEEEEECCHHHHHHHH
AFGPLILGHAHPEVVEAVVERVREGFHYGTPTLPELKLAEKVVELVPNVEKVRLVNTGTE
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEEECCHH
ATMSAIRLARAYTGREKIVKFEGCYHGAHDAVLVRAGSGASELGAPDSPGIPESVAENTL
HHHHHHHHHHHHCCHHHEEEECCCCCCCCCEEEEEECCCHHHCCCCCCCCCCHHHHCCCE
VCPFNDVEAFVETVERFDEEIGAVIVEPVLGNAGCVPPDEEFLKVLREYCDGTERLLIFD
ECCCHHHHHHHHHHHHHHHHHCCEEEHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHH
EVITGFRLELGGAQEYYGIDADLVCLGKILGGGLPIGAFGGPEEYMSRVAPEGKVYQAGT
HHHCCCEEEECCCCHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEECCC
FNGNPVSATAGLVTLEVLERERPYDELSSKAERLASALEDGLEDRGIEGVVNRVESMFQV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YFGIEEVRDYADVNSADHDAFKRFHRELLEHGVWIAASNYEAWFLSIAHTETDLERTEEA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEEEEHHHHHHHHHHHHH
FEEALDRLTG
HHHHHHHHCC
>Mature Secondary Structure 
GYEDEFPESLELFKRAERVMPGGVSSPVRRFDPYPFYVERAEGSRLYTVDGHVLIDYCL
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEECCCCEEEEECCHHHHHHHH
AFGPLILGHAHPEVVEAVVERVREGFHYGTPTLPELKLAEKVVELVPNVEKVRLVNTGTE
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEEECCHH
ATMSAIRLARAYTGREKIVKFEGCYHGAHDAVLVRAGSGASELGAPDSPGIPESVAENTL
HHHHHHHHHHHHCCHHHEEEECCCCCCCCCEEEEEECCCHHHCCCCCCCCCCHHHHCCCE
VCPFNDVEAFVETVERFDEEIGAVIVEPVLGNAGCVPPDEEFLKVLREYCDGTERLLIFD
ECCCHHHHHHHHHHHHHHHHHCCEEEHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHH
EVITGFRLELGGAQEYYGIDADLVCLGKILGGGLPIGAFGGPEEYMSRVAPEGKVYQAGT
HHHCCCEEEECCCCHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEECCC
FNGNPVSATAGLVTLEVLERERPYDELSSKAERLASALEDGLEDRGIEGVVNRVESMFQV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YFGIEEVRDYADVNSADHDAFKRFHRELLEHGVWIAASNYEAWFLSIAHTETDLERTEEA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEEEEHHHHHHHHHHHHH
FEEALDRLTG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014