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The map label for this gene is leuA2

Identifier: 20093715

GI number: 20093715

Start: 263828

End: 265357

Strand: Reverse

Name: leuA2

Synonym: MK0275

Alternate gene names: 20093715

Gene position: 265357-263828 (Counterclockwise)

Preceding gene: 20093716

Following gene: 20093712

Centisome position: 15.66

GC content: 63.4

Gene sequence:

>1530_bases
TTGAGAGAGGCCAACGCGGACGCGGACCCGCCGGACGAGGTGAGGATATTCGACACCACTCTGAGGGACGGGGAGCAGAC
CCCGGGAGTCGCTCTGACCCCCGAGGAAAAGCTGCGGATCGCCCGAAAGCTCGACGAGATCGGCGTGGATACGATCGAAG
CCGGCTTCGCGGCCGCGAGTGAGGGAGAACTCAAGGCGATTCGGCGCATCGCCCGGGAGGAGCTCGACGCCGAAGTGTGC
AGTATGGCGCGTATGGTGAAGGGTGACGTGGACGCGGCGGTCGAAGCCGAGGCCGACGCGGTTCACATCGTCGTACCTAC
TTCCGAGGTCCACGTGAAGAAGAAGCTTCGGATGGATCGTGAAGAGGTCCTGGAGCGCGCTCGCGAGGTCGTAGAGTACG
CGCGCGATCACGGGTTGACGGTGGAGATTTCCACCGAAGACGGTACCAGGACGGAGCTCGAGTACCTGTACGAGGTGTTC
GACGCCTGCCTGGAGGCGGGCGCCGAACGTCTCGGTTACAACGACACCGTCGGAGTGATGGCGCCGGAGGGTATGTTCCT
GGCGGTGAAGAAGCTCCGGGAGCGCGTCGGTGAGGACGTCATCCTCAGCGTGCACTGTCACGATGACTTCGGTATGGCGA
CGGCCAACACCGTCGCCGCCGTCAGGGCGGGGGCGCGTCAGGTCCACGTGACCGTGAACGGGATCGGCGAGCGCGCCGGC
AACGCGGCGTTGGAGGAGGTGGTGGTAGTGCTGGAGGAGCTGTACGGCGTGGACACCGGCATCCGGACCGAGCGGCTGAC
GGAGCTCTCCAAGCTCGTGGAGAGGCTGACCGGCGTTAGGGTACCACCGAACAAGGCCGTGGTAGGTGAGAACGCTTTCA
CCCACGAGTCGGGGATCCACGCCGACGGCATCCTAAAGGACGAGAGTACTTACGAGCCCATTCCGCCCGAGAAAGTCGGG
CATGAACGCCGGTTCGTCCTAGGTAAACACGTAGGAACAAGCGTGATCCGTAAGAAGTTGAAACAAATGGGTGTCGACGT
CGACGACGAGCAGCTCCTGGAGATCCTCCGACGCCTCAAGCGGTTGGGGGACCGTGGGAAGCGGATCACCGAGGCCGATC
TCCGCGCGATCGCCGAGGACGTACTGGGGAGACCGGCTGAGCGTGACATCGAGGTCGAGGATTTCACGACGGTCACCGGG
AAGAGGACTATACCGACGGCCTCGATCGTCGTGAAGATCGACGGGACCAGGAAGGAGGCCGCGTCTACCGGCGTGGGACC
CGTGGACGCTACTATCAAGGCGTTGGAGCGGGCCCTCAAGGATCAAGGGATCGACTTCGAGCTCGTCGAGTATCGGGCTG
AGGCGCTGACAGGCGGTACCGACGCTATCACCCACGTGGATGTCAAGCTGCGCGATCCGGAGACCGGTGATATAGTGCAT
TCCGGCTCCTCACGGGAAGACATCGTCGTGGCGAGCTTGGAGGCGTTCATCGACGGTATCAACTCGCTGATGGCCCGCAA
GCGTAGCTGA

Upstream 100 bases:

>100_bases
AGCTTGAAGAAGCGTACCGGGAGCTCATCGGTGCCGTCCAGGAGAGGCTGTAGGGGCGTGCCTGACGTGGGGGATCGCTT
TATCCGCCGTTAGGGAGCTG

Downstream 100 bases:

>100_bases
TCAGGCCAAGCCCCACTGCCTGATTATCTCCTCCATCCCACGTCTGTTCTCCCAGAACCTACGCAGACCTTGAAGGTAAC
CCCTCCACGTCGGGTCGTAA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MREANADADPPDEVRIFDTTLRDGEQTPGVALTPEEKLRIARKLDEIGVDTIEAGFAAASEGELKAIRRIAREELDAEVC
SMARMVKGDVDAAVEAEADAVHIVVPTSEVHVKKKLRMDREEVLERAREVVEYARDHGLTVEISTEDGTRTELEYLYEVF
DACLEAGAERLGYNDTVGVMAPEGMFLAVKKLRERVGEDVILSVHCHDDFGMATANTVAAVRAGARQVHVTVNGIGERAG
NAALEEVVVVLEELYGVDTGIRTERLTELSKLVERLTGVRVPPNKAVVGENAFTHESGIHADGILKDESTYEPIPPEKVG
HERRFVLGKHVGTSVIRKKLKQMGVDVDDEQLLEILRRLKRLGDRGKRITEADLRAIAEDVLGRPAERDIEVEDFTTVTG
KRTIPTASIVVKIDGTRKEAASTGVGPVDATIKALERALKDQGIDFELVEYRAEALTGGTDAITHVDVKLRDPETGDIVH
SGSSREDIVVASLEAFIDGINSLMARKRS

Sequences:

>Translated_509_residues
MREANADADPPDEVRIFDTTLRDGEQTPGVALTPEEKLRIARKLDEIGVDTIEAGFAAASEGELKAIRRIAREELDAEVC
SMARMVKGDVDAAVEAEADAVHIVVPTSEVHVKKKLRMDREEVLERAREVVEYARDHGLTVEISTEDGTRTELEYLYEVF
DACLEAGAERLGYNDTVGVMAPEGMFLAVKKLRERVGEDVILSVHCHDDFGMATANTVAAVRAGARQVHVTVNGIGERAG
NAALEEVVVVLEELYGVDTGIRTERLTELSKLVERLTGVRVPPNKAVVGENAFTHESGIHADGILKDESTYEPIPPEKVG
HERRFVLGKHVGTSVIRKKLKQMGVDVDDEQLLEILRRLKRLGDRGKRITEADLRAIAEDVLGRPAERDIEVEDFTTVTG
KRTIPTASIVVKIDGTRKEAASTGVGPVDATIKALERALKDQGIDFELVEYRAEALTGGTDAITHVDVKLRDPETGDIVH
SGSSREDIVVASLEAFIDGINSLMARKRS
>Mature_509_residues
MREANADADPPDEVRIFDTTLRDGEQTPGVALTPEEKLRIARKLDEIGVDTIEAGFAAASEGELKAIRRIAREELDAEVC
SMARMVKGDVDAAVEAEADAVHIVVPTSEVHVKKKLRMDREEVLERAREVVEYARDHGLTVEISTEDGTRTELEYLYEVF
DACLEAGAERLGYNDTVGVMAPEGMFLAVKKLRERVGEDVILSVHCHDDFGMATANTVAAVRAGARQVHVTVNGIGERAG
NAALEEVVVVLEELYGVDTGIRTERLTELSKLVERLTGVRVPPNKAVVGENAFTHESGIHADGILKDESTYEPIPPEKVG
HERRFVLGKHVGTSVIRKKLKQMGVDVDDEQLLEILRRLKRLGDRGKRITEADLRAIAEDVLGRPAERDIEVEDFTTVTG
KRTIPTASIVVKIDGTRKEAASTGVGPVDATIKALERALKDQGIDFELVEYRAEALTGGTDAITHVDVKLRDPETGDIVH
SGSSREDIVVASLEAFIDGINSLMARKRS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=287, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI109150422, Length=301, Percent_Identity=25.9136212624585, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI109150427, Length=301, Percent_Identity=25.9136212624585, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786261, Length=504, Percent_Identity=36.7063492063492, Blast_Score=304, Evalue=8e-84,
Organism=Saccharomyces cerevisiae, GI6320019, Length=411, Percent_Identity=30.9002433090024, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6320071, Length=356, Percent_Identity=32.3033707865169, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324682, Length=547, Percent_Identity=23.7659963436929, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324225, Length=555, Percent_Identity=24.6846846846847, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319695, Length=124, Percent_Identity=32.258064516129, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582381, Length=286, Percent_Identity=29.020979020979, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU12_METKA (Q8TYM1)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613562.1
- ProteinModelPortal:   Q8TYM1
- SMR:   Q8TYM1
- GeneID:   1477578
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0275
- NMPDR:   fig|190192.1.peg.275
- HOGENOM:   HBG701974
- OMA:   CFCDTVG
- ProtClustDB:   PRK00915
- BioCyc:   MKAN190192:MK0275-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_A
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55688; Mature: 55688

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREANADADPPDEVRIFDTTLRDGEQTPGVALTPEEKLRIARKLDEIGVDTIEAGFAAAS
CCCCCCCCCCCCCEEEEEEHHCCCCCCCCEEECCHHHHHHHHHHHHHCCHHHHHCCCCCC
EGELKAIRRIAREELDAEVCSMARMVKGDVDAAVEAEADAVHIVVPTSEVHVKKKLRMDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCEEEEEECCHHHHHHHHHCCCH
EEVLERAREVVEYARDHGLTVEISTEDGTRTELEYLYEVFDACLEAGAERLGYNDTVGVM
HHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
APEGMFLAVKKLRERVGEDVILSVHCHDDFGMATANTVAAVRAGARQVHVTVNGIGERAG
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCC
NAALEEVVVVLEELYGVDTGIRTERLTELSKLVERLTGVRVPPNKAVVGENAFTHESGIH
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
ADGILKDESTYEPIPPEKVGHERRFVLGKHVGTSVIRKKLKQMGVDVDDEQLLEILRRLK
CCCEECCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RLGDRGKRITEADLRAIAEDVLGRPAERDIEVEDFTTVTGKRTIPTASIVVKIDGTRKEA
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCCCCEEEEEEECCCHHHH
ASTGVGPVDATIKALERALKDQGIDFELVEYRAEALTGGTDAITHVDVKLRDPETGDIVH
HCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEE
SGSSREDIVVASLEAFIDGINSLMARKRS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MREANADADPPDEVRIFDTTLRDGEQTPGVALTPEEKLRIARKLDEIGVDTIEAGFAAAS
CCCCCCCCCCCCCEEEEEEHHCCCCCCCCEEECCHHHHHHHHHHHHHCCHHHHHCCCCCC
EGELKAIRRIAREELDAEVCSMARMVKGDVDAAVEAEADAVHIVVPTSEVHVKKKLRMDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCEEEEEECCHHHHHHHHHCCCH
EEVLERAREVVEYARDHGLTVEISTEDGTRTELEYLYEVFDACLEAGAERLGYNDTVGVM
HHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
APEGMFLAVKKLRERVGEDVILSVHCHDDFGMATANTVAAVRAGARQVHVTVNGIGERAG
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCC
NAALEEVVVVLEELYGVDTGIRTERLTELSKLVERLTGVRVPPNKAVVGENAFTHESGIH
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
ADGILKDESTYEPIPPEKVGHERRFVLGKHVGTSVIRKKLKQMGVDVDDEQLLEILRRLK
CCCEECCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RLGDRGKRITEADLRAIAEDVLGRPAERDIEVEDFTTVTGKRTIPTASIVVKIDGTRKEA
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCCCCEEEEEEECCCHHHH
ASTGVGPVDATIKALERALKDQGIDFELVEYRAEALTGGTDAITHVDVKLRDPETGDIVH
HCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEE
SGSSREDIVVASLEAFIDGINSLMARKRS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014