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The map label for this gene is PurA [H]

Identifier: 20093675

GI number: 20093675

Start: 220673

End: 221713

Strand: Reverse

Name: PurA [H]

Synonym: MK0235

Alternate gene names: 20093675

Gene position: 221713-220673 (Counterclockwise)

Preceding gene: 20093676

Following gene: 20093671

Centisome position: 13.08

GC content: 65.8

Gene sequence:

>1041_bases
TTGCGGGATCGCGGTCACGTTAGCGTCGTTGCTGGTGGTCAGTGGGGCGACGAGGGCAAGGGCAAGATCGTGGCGTACCT
CGCGGTCCAGGACGAGCCGGAGGTCATAGCGCGGGCCGGTGTCGGTCCCAACGCCGGCCACACCGTGCGCGTGAATGGGG
AGGATTACGGCCTCCGGCAGATCCCGTGCGGCTTCCCACACGAGGAGGCCGAGCTGGCCATCGGACCCGGGGTTCTCGTC
AACCCCGAAGTGCTCTTGGACGAAGTCGAGCGACTGTCCCGCTTCCGGGTCGATGACCGCCTGATCGTGGATGAGAGGTG
CGCGATCATCGAGCCCAAGCACATCGAGGCCGAGCGCGCTTCCAAGCACCTCTCGGACGAGATCGACACGACCGGCACCG
GCTGCGGGCCGGCGAACGCGGACAGGGCGTTGCGGAAGGCGAAGCTGGCCCGGGACGTCGACGAGCTTTCGGAGTTCCTC
GGCGACGTTCCCGGGTTGGTGAACGAGGCTATCGACGCGGGCGAGGACGTGCTGATCGAGGGTACGCAGGGGTTCGGTCT
ATCCCTATACCACGGGATCGACTACCCTTATGTCACCAGCAAGGATACCACGGCGAGCGCCTTCGCCTCCGACGTCGGCG
TCGGCCCGACGAGGATCGACGACGTGTACGTGGTGTTCAAGGCGTACGCAACCCGGGTCGGGGAAGGACCGTTCCCGACG
GAGTTGTCCCGGGAGGAGGTGATAGAGAAGTTCGGCGAGGAGATCCTGGAGGTCGAGCGCGGTACGGTCACGGGTCGGCC
GCGACGCATCGGCGAGTTCGACTTCGAGATGGCCAAGCGCGCGTGCGTCATCAACGGCGCCACTCAGGTCGCGATCACGT
GCATCGACAGGAGGTTTCCGGACGCGGCGGAAGCCGAGACTTGGCACGAGCTTCCCACCGAGGCGAAGAAGTTCGTCGAG
AAGGTGGAGGAGGCCGTCGGGGTACCCGTGACGATAGTATCCACGGGACCGGAGCTGGAGCACACCGTGGACCTAAGGTA
G

Upstream 100 bases:

>100_bases
TGAACGAATCCGATAAGTACCATGTCACGGTCACGCTGGAGCTCGTCACGCCACAGCCCTCTCCGCCGGCCTCCTGGCGG
AAGGTTCCGGGGGTATCGGT

Downstream 100 bases:

>100_bases
TTCGTCCAGGATCCGCCGGACCCGATCGCTCACGGGCACCTCGACCACCTCGAACCGTTCCCTTTTCTCCACCGGGGCCG
TCGCGTCGAACACGGCCTTC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRDRGHVSVVAGGQWGDEGKGKIVAYLAVQDEPEVIARAGVGPNAGHTVRVNGEDYGLRQIPCGFPHEEAELAIGPGVLV
NPEVLLDEVERLSRFRVDDRLIVDERCAIIEPKHIEAERASKHLSDEIDTTGTGCGPANADRALRKAKLARDVDELSEFL
GDVPGLVNEAIDAGEDVLIEGTQGFGLSLYHGIDYPYVTSKDTTASAFASDVGVGPTRIDDVYVVFKAYATRVGEGPFPT
ELSREEVIEKFGEEILEVERGTVTGRPRRIGEFDFEMAKRACVINGATQVAITCIDRRFPDAAEAETWHELPTEAKKFVE
KVEEAVGVPVTIVSTGPELEHTVDLR

Sequences:

>Translated_346_residues
MRDRGHVSVVAGGQWGDEGKGKIVAYLAVQDEPEVIARAGVGPNAGHTVRVNGEDYGLRQIPCGFPHEEAELAIGPGVLV
NPEVLLDEVERLSRFRVDDRLIVDERCAIIEPKHIEAERASKHLSDEIDTTGTGCGPANADRALRKAKLARDVDELSEFL
GDVPGLVNEAIDAGEDVLIEGTQGFGLSLYHGIDYPYVTSKDTTASAFASDVGVGPTRIDDVYVVFKAYATRVGEGPFPT
ELSREEVIEKFGEEILEVERGTVTGRPRRIGEFDFEMAKRACVINGATQVAITCIDRRFPDAAEAETWHELPTEAKKFVE
KVEEAVGVPVTIVSTGPELEHTVDLR
>Mature_346_residues
MRDRGHVSVVAGGQWGDEGKGKIVAYLAVQDEPEVIARAGVGPNAGHTVRVNGEDYGLRQIPCGFPHEEAELAIGPGVLV
NPEVLLDEVERLSRFRVDDRLIVDERCAIIEPKHIEAERASKHLSDEIDTTGTGCGPANADRALRKAKLARDVDELSEFL
GDVPGLVNEAIDAGEDVLIEGTQGFGLSLYHGIDYPYVTSKDTTASAFASDVGVGPTRIDDVYVVFKAYATRVGEGPFPT
ELSREEVIEKFGEEILEVERGTVTGRPRRIGEFDFEMAKRACVINGATQVAITCIDRRFPDAAEAETWHELPTEAKKFVE
KVEEAVGVPVTIVSTGPELEHTVDLR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=433, Percent_Identity=30.0230946882217, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI34577063, Length=434, Percent_Identity=27.4193548387097, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=28.1407035175879, Blast_Score=121, Evalue=8e-28,
Organism=Escherichia coli, GI1790620, Length=434, Percent_Identity=32.9493087557604, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=30.5361305361305, Blast_Score=173, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=30.5361305361305, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324109, Length=436, Percent_Identity=30.5045871559633, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21356033, Length=433, Percent_Identity=27.2517321016166, Blast_Score=148, Evalue=7e-36,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 37632; Mature: 37632

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDRGHVSVVAGGQWGDEGKGKIVAYLAVQDEPEVIARAGVGPNAGHTVRVNGEDYGLRQ
CCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHHEECCCCCCCCCEEEECCCCCCCEE
IPCGFPHEEAELAIGPGVLVNPEVLLDEVERLSRFRVDDRLIVDERCAIIEPKHIEAERA
CCCCCCCCCCCEEECCCEEECHHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCCHHHH
SKHLSDEIDTTGTGCGPANADRALRKAKLARDVDELSEFLGDVPGLVNEAIDAGEDVLIE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEEE
GTQGFGLSLYHGIDYPYVTSKDTTASAFASDVGVGPTRIDDVYVVFKAYATRVGEGPFPT
CCCCCCEEEEECCCCCEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
ELSREEVIEKFGEEILEVERGTVTGRPRRIGEFDFEMAKRACVINGATQVAITCIDRRFP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHEEEECCCCEEEEEEECCCCC
DAAEAETWHELPTEAKKFVEKVEEAVGVPVTIVSTGPELEHTVDLR
CCHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCC
>Mature Secondary Structure
MRDRGHVSVVAGGQWGDEGKGKIVAYLAVQDEPEVIARAGVGPNAGHTVRVNGEDYGLRQ
CCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHHEECCCCCCCCCEEEECCCCCCCEE
IPCGFPHEEAELAIGPGVLVNPEVLLDEVERLSRFRVDDRLIVDERCAIIEPKHIEAERA
CCCCCCCCCCCEEECCCEEECHHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCCHHHH
SKHLSDEIDTTGTGCGPANADRALRKAKLARDVDELSEFLGDVPGLVNEAIDAGEDVLIE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEEE
GTQGFGLSLYHGIDYPYVTSKDTTASAFASDVGVGPTRIDDVYVVFKAYATRVGEGPFPT
CCCCCCEEEEECCCCCEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
ELSREEVIEKFGEEILEVERGTVTGRPRRIGEFDFEMAKRACVINGATQVAITCIDRRFP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHEEEECCCCEEEEEEECCCCC
DAAEAETWHELPTEAKKFVEKVEEAVGVPVTIVSTGPELEHTVDLR
CCHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA