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The map label for this gene is selB [H]

Identifier: 20093588

GI number: 20093588

Start: 147400

End: 148779

Strand: Reverse

Name: selB [H]

Synonym: MK0148

Alternate gene names: 20093588

Gene position: 148779-147400 (Counterclockwise)

Preceding gene: 20093589

Following gene: 20093586

Centisome position: 8.78

GC content: 59.2

Gene sequence:

>1380_bases
GTGCCCGTAGTACATGTTGGACTGTTCGGACACATCGACCACGGCAAGACGGCACTCGCAGCGCAGCTGACTGAGAAGCC
TTCTACGGCGGCGTTGGATAAACACCCGGAGGAGAAGGAGCGTGGGATTACGATAGACCTTGGGTTCTCCAGCTTCGAGC
TCGGTGATTACACCGTCACACTGGTAGACGCCCCGGGCCACGCTGATCTCATCAGAACCGTGGTGGCCGGGGCCGAAATA
ATCGACGCCGCGATCTTGGTCGTAGCGGCCGATGAGGGCCCTCAGGTACAGACCGGGGAACACCTGGTGGTGCTTAATCA
CCTGGGTATCGACCGTGGGGTCATCGCCCTCAATAAGGTGGACCTGGTGGACGAAAAGACCGTCGAGCGTCGGATCGAGG
AGATCAAACGAGTACTCCAGGGGACCACCTTAGAAGACGCTCCCATAATCCCGGTCTCCGCGAAGATCGGTGAGGGTATT
GAGGATCTGAAAGACGCGTTGCTCGAGGTGCTGGAACCTCCCAATCGTGATCTAGATTCTCCGTTCAGAATGCCGATAGA
CCACGCGTTCCACGTCAAAGGTGCCGGGACCGTGGTAACCGGTACCGTACTGACCGGACGTGTCGAGGTCGGGGATGAAC
TGACACTGTACCCGATCGGCAAGACCGTCGAAGTGAAGTCCATCCAGAGCTTCGGGAAGGACAAGCAGGAAGCGTGTGCG
GGTGATCGGGTTGGAATAGCGCTACGAGGGATCCGAGAGGAAGAGATCGAACGGGGCTTCCAACTGGCCGAGGAGGGTTC
CCTGCGGGTCACCAGATACCTTGACTTGAAGGTGGAAATAGACCCCCTGTTCCCCCAATCCATCGGGCAGAAGACCATGC
TGCACATACACGTCGGAATGAGGTCCGTACCGGCTCGGATCGTGCCTCACGACGATGGGTTCCTCCTAGACTCCCTACGA
CCCGGCGAATCGAGTTACCTGTATGCGAAGCTCAACGAGCCCGTGGCCGTGAGGGAGGGGGATAGGACGATCCTCGTTAA
GTTGGACTTACCGCCGACAACGCTCCGCATAGCGGGCTCCGGGCTCGTGGAGGATACCTCGAAGCGTGAGACATTCAAGC
GCGTCTCCAGGCGACGTGGGCGCGTTACAAGGGCCGACCATATGGGGAAGGGCTTAGCGGTCGTCGATGGACTAGCTTTA
AACAAGGAGCACGCCGAGCGTCTGGTGGGCGAGAAAGTCCGCACGGAGGGAGGAGTCGAGGGGAAAATCGTGGACACTCA
CGGTACGCGCGGGGCCGTACTAGTCGACTTCGAGGGGGAGGTTAAAACAGGGGAACGGGTCGTTCTGGAGCGGGTTCGGG
ACGTCAAAATCGACCTCTGA

Upstream 100 bases:

>100_bases
GGAAGACATTTTCACAGCGGCCGCAGTGGCGGGAGCCTCATCGCTCTTCAATCGGGCGAAGGTGCGATACTTCTTCGACG
AAGGGTGGTGATGCAGCGCC

Downstream 100 bases:

>100_bases
CCTCCTTGTCCTTGAAGGATGCCCTCGAAGCATCAGCGCTGGAACGTCTCTTCACTAAGTCCGCTCCCGAGGGGTTCATC
ACCCGAAGTACCTCTTGGCG

Product: GTPase - translation elongation factor

Products: GDP; phosphate

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MPVVHVGLFGHIDHGKTALAAQLTEKPSTAALDKHPEEKERGITIDLGFSSFELGDYTVTLVDAPGHADLIRTVVAGAEI
IDAAILVVAADEGPQVQTGEHLVVLNHLGIDRGVIALNKVDLVDEKTVERRIEEIKRVLQGTTLEDAPIIPVSAKIGEGI
EDLKDALLEVLEPPNRDLDSPFRMPIDHAFHVKGAGTVVTGTVLTGRVEVGDELTLYPIGKTVEVKSIQSFGKDKQEACA
GDRVGIALRGIREEEIERGFQLAEEGSLRVTRYLDLKVEIDPLFPQSIGQKTMLHIHVGMRSVPARIVPHDDGFLLDSLR
PGESSYLYAKLNEPVAVREGDRTILVKLDLPPTTLRIAGSGLVEDTSKRETFKRVSRRRGRVTRADHMGKGLAVVDGLAL
NKEHAERLVGEKVRTEGGVEGKIVDTHGTRGAVLVDFEGEVKTGERVVLERVRDVKIDL

Sequences:

>Translated_459_residues
MPVVHVGLFGHIDHGKTALAAQLTEKPSTAALDKHPEEKERGITIDLGFSSFELGDYTVTLVDAPGHADLIRTVVAGAEI
IDAAILVVAADEGPQVQTGEHLVVLNHLGIDRGVIALNKVDLVDEKTVERRIEEIKRVLQGTTLEDAPIIPVSAKIGEGI
EDLKDALLEVLEPPNRDLDSPFRMPIDHAFHVKGAGTVVTGTVLTGRVEVGDELTLYPIGKTVEVKSIQSFGKDKQEACA
GDRVGIALRGIREEEIERGFQLAEEGSLRVTRYLDLKVEIDPLFPQSIGQKTMLHIHVGMRSVPARIVPHDDGFLLDSLR
PGESSYLYAKLNEPVAVREGDRTILVKLDLPPTTLRIAGSGLVEDTSKRETFKRVSRRRGRVTRADHMGKGLAVVDGLAL
NKEHAERLVGEKVRTEGGVEGKIVDTHGTRGAVLVDFEGEVKTGERVVLERVRDVKIDL
>Mature_458_residues
PVVHVGLFGHIDHGKTALAAQLTEKPSTAALDKHPEEKERGITIDLGFSSFELGDYTVTLVDAPGHADLIRTVVAGAEII
DAAILVVAADEGPQVQTGEHLVVLNHLGIDRGVIALNKVDLVDEKTVERRIEEIKRVLQGTTLEDAPIIPVSAKIGEGIE
DLKDALLEVLEPPNRDLDSPFRMPIDHAFHVKGAGTVVTGTVLTGRVEVGDELTLYPIGKTVEVKSIQSFGKDKQEACAG
DRVGIALRGIREEEIERGFQLAEEGSLRVTRYLDLKVEIDPLFPQSIGQKTMLHIHVGMRSVPARIVPHDDGFLLDSLRP
GESSYLYAKLNEPVAVREGDRTILVKLDLPPTTLRIAGSGLVEDTSKRETFKRVSRRRGRVTRADHMGKGLAVVDGLALN
KEHAERLVGEKVRTEGGVEGKIVDTHGTRGAVLVDFEGEVKTGERVVLERVRDVKIDL

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI54607086, Length=371, Percent_Identity=37.466307277628, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI34147630, Length=398, Percent_Identity=31.1557788944724, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4503475, Length=316, Percent_Identity=28.7974683544304, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4503471, Length=316, Percent_Identity=29.1139240506329, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI223555963, Length=300, Percent_Identity=31, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI5729864, Length=300, Percent_Identity=31, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4503507, Length=305, Percent_Identity=28.1967213114754, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI194097354, Length=354, Percent_Identity=28.2485875706215, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI194018520, Length=354, Percent_Identity=28.2485875706215, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI46094014, Length=354, Percent_Identity=28.5310734463277, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI194018522, Length=354, Percent_Identity=28.2485875706215, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI157426893, Length=268, Percent_Identity=27.6119402985075, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI82546879, Length=460, Percent_Identity=24.1304347826087, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI45593140, Length=308, Percent_Identity=26.2987012987013, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI2367247, Length=303, Percent_Identity=38.2838283828383, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1790412, Length=386, Percent_Identity=33.6787564766839, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789737, Length=386, Percent_Identity=33.6787564766839, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789108, Length=236, Percent_Identity=33.0508474576271, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994988, Length=234, Percent_Identity=28.2051282051282, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788922, Length=240, Percent_Identity=27.5, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17506081, Length=461, Percent_Identity=27.5488069414317, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25141371, Length=307, Percent_Identity=34.2019543973941, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17552884, Length=316, Percent_Identity=29.1139240506329, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17569207, Length=316, Percent_Identity=29.1139240506329, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17556456, Length=294, Percent_Identity=34.0136054421769, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17506079, Length=460, Percent_Identity=25.4347826086956, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17509919, Length=261, Percent_Identity=30.6513409961686, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566303, Length=309, Percent_Identity=27.1844660194175, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI115532067, Length=305, Percent_Identity=31.1475409836066, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115532065, Length=305, Percent_Identity=31.1475409836066, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32566629, Length=357, Percent_Identity=27.7310924369748, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=236, Percent_Identity=30.0847457627119, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=215, Percent_Identity=29.3023255813954, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=293, Percent_Identity=39.2491467576792, Blast_Score=183, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6325337, Length=314, Percent_Identity=30.2547770700637, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6319594, Length=314, Percent_Identity=30.2547770700637, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6320863, Length=261, Percent_Identity=27.5862068965517, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322937, Length=381, Percent_Identity=25.9842519685039, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323320, Length=295, Percent_Identity=29.1525423728814, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320377, Length=357, Percent_Identity=25.7703081232493, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324550, Length=174, Percent_Identity=31.0344827586207, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922690, Length=501, Percent_Identity=31.936127744511, Blast_Score=221, Evalue=9e-58,
Organism=Drosophila melanogaster, GI19921738, Length=405, Percent_Identity=33.3333333333333, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI281363316, Length=417, Percent_Identity=32.1342925659472, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI17864358, Length=417, Percent_Identity=32.1342925659472, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI45553807, Length=318, Percent_Identity=28.9308176100629, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45553816, Length=318, Percent_Identity=28.9308176100629, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24651721, Length=318, Percent_Identity=28.9308176100629, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17864154, Length=318, Percent_Identity=28.9308176100629, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24652838, Length=318, Percent_Identity=29.2452830188679, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17137572, Length=318, Percent_Identity=29.2452830188679, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24647046, Length=261, Percent_Identity=29.5019157088123, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647048, Length=261, Percent_Identity=29.5019157088123, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17137380, Length=359, Percent_Identity=28.4122562674095, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45550900, Length=344, Percent_Identity=26.7441860465116, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI78706572, Length=245, Percent_Identity=30.6122448979592, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 50007; Mature: 49876

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVHVGLFGHIDHGKTALAAQLTEKPSTAALDKHPEEKERGITIDLGFSSFELGDYTVT
CCEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCEEEEECCCEEECCCEEEE
LVDAPGHADLIRTVVAGAEIIDAAILVVAADEGPQVQTGEHLVVLNHLGIDRGVIALNKV
EEECCCHHHHHHHHHHCHHHHEEEEEEEECCCCCCEECCCEEEEEEECCCCCCEEEEEEE
DLVDEKTVERRIEEIKRVLQGTTLEDAPIIPVSAKIGEGIEDLKDALLEVLEPPNRDLDS
CCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHCCCHHHHHHHHHHHHCCCCCCCCC
PFRMPIDHAFHVKGAGTVVTGTVLTGRVEVGDELTLYPIGKTVEVKSIQSFGKDKQEACA
CCCCCCCCEEEECCCCEEEEEEEEEEEEECCCEEEEEECCCEEEHHHHHHHCCCHHHHHC
GDRVGIALRGIREEEIERGFQLAEEGSLRVTRYLDLKVEIDPLFPQSIGQKTMLHIHVGM
CCCCEEEEECCCHHHHHHHHHECCCCCEEEEEEEEEEEEECCCCCCCCCCCEEEEEEECC
RSVPARIVPHDDGFLLDSLRPGESSYLYAKLNEPVAVREGDRTILVKLDLPPTTLRIAGS
CCCCCEEEECCCCEEECCCCCCCCCEEEEECCCCEEEECCCEEEEEEECCCCCEEEEECC
GLVEDTSKRETFKRVSRRRGRVTRADHMGKGLAVVDGLALNKEHAERLVGEKVRTEGGVE
CCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCEECCHHHHHHHHHHHHHCCCCCC
GKIVDTHGTRGAVLVDFEGEVKTGERVVLERVRDVKIDL
EEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCEECC
>Mature Secondary Structure 
PVVHVGLFGHIDHGKTALAAQLTEKPSTAALDKHPEEKERGITIDLGFSSFELGDYTVT
CEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCEEEEECCCEEECCCEEEE
LVDAPGHADLIRTVVAGAEIIDAAILVVAADEGPQVQTGEHLVVLNHLGIDRGVIALNKV
EEECCCHHHHHHHHHHCHHHHEEEEEEEECCCCCCEECCCEEEEEEECCCCCCEEEEEEE
DLVDEKTVERRIEEIKRVLQGTTLEDAPIIPVSAKIGEGIEDLKDALLEVLEPPNRDLDS
CCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHCCCHHHHHHHHHHHHCCCCCCCCC
PFRMPIDHAFHVKGAGTVVTGTVLTGRVEVGDELTLYPIGKTVEVKSIQSFGKDKQEACA
CCCCCCCCEEEECCCCEEEEEEEEEEEEECCCEEEEEECCCEEEHHHHHHHCCCHHHHHC
GDRVGIALRGIREEEIERGFQLAEEGSLRVTRYLDLKVEIDPLFPQSIGQKTMLHIHVGM
CCCCEEEEECCCHHHHHHHHHECCCCCEEEEEEEEEEEEECCCCCCCCCCCEEEEEEECC
RSVPARIVPHDDGFLLDSLRPGESSYLYAKLNEPVAVREGDRTILVKLDLPPTTLRIAGS
CCCCCEEEECCCCEEECCCCCCCCCEEEEECCCCEEEECCCEEEEEEECCCCCEEEEECC
GLVEDTSKRETFKRVSRRRGRVTRADHMGKGLAVVDGLALNKEHAERLVGEKVRTEGGVE
CCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCEECCHHHHHHHHHHHHHCCCCCC
GKIVDTHGTRGAVLVDFEGEVKTGERVVLERVRDVKIDL
EEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 10860743 [H]