Click here to switch to the map view.

The map label for this gene is ypfI [C]

Identifier: 20093586

GI number: 20093586

Start: 143918

End: 146485

Strand: Reverse

Name: ypfI [C]

Synonym: MK0146

Alternate gene names: 20093586

Gene position: 146485-143918 (Counterclockwise)

Preceding gene: 20093588

Following gene: 20093583

Centisome position: 8.64

GC content: 60.01

Gene sequence:

>2568_bases
ATGCCGGACAAGGCGGAAGTGTTCTTCGACGAGGGCGTGCTGGACTTCGCGGACGTCGATGAGATCGTGCTCGACGTGGG
GGAGGAAGCCCTCGCCGAGGCCTTAGCCCACCGGCACCGAAGGATGATCGTGTTTCAGGGTGATGAGGGCAAAGCGGAGG
CCGCGGGTGTCGTGACGGCGGGAGCTGCGGACGTCCTTTTCGACGTCCGGGACAGACCCATTTCCGTCCTGTACGTGACG
GACAGTCTCAAGGAGGATACCTACGCTCGTGAGCGTTACGAGGAGTTTCGCCGCGTCCTCGAGGGGTTCGCCGAGGAAGC
GAACTTCGAGTACGAATTAGAAGCGCTCACTTTCAGCGGCTCCAAACGGGCGCTCGGTACCACCTGGGACCTCATGGTGA
TCGACCTCTCCTACGACCTGGATCCGGACGCTATCGGTCGTCTCGTCGAAACCGTTCGTGGAGGTGGTCTCGTAATCTTC
CAGACCCCGCCCTTCGACCGCTGGAGGAACATGTGGACGGCCTTCCACAAGAGCTTAGTGACACCACCTTACACGCTCGA
TCACGTCGGTAAGCGCTTCAACCGGCGGTTCATCAGGAAGCTGAAGGAGCACGACGGTGTTTGGATAGTGGATACGGACG
AGTGGACCGCGGAGCCCGAACCGTCGGAAGATGTCGACCTCGAGGTGGAGGTTAAGCGCCGAGAGCGGCCAGATCTCGAT
CCACCGGACGACGCCGTGCTTCCCGAAGAGCTCTATCGGATGTGTGCAACCGAGGATCAGTTCCGGGCTTTGATCAGGTT
CGAAGAGCTGCTGGAGTCGAACGGTAAGACCGCCCTCATCCTCACGGCGGACCGAGGGCGGGGCAAGTCGGCGCTTCTCG
GAATCGCCGTGGCCGGTGCGGGCGTCACGACCGACGTGTACGACGTCGTGGTGACGGCGTCCGAGCCCGAGAACGTCGCC
GTACTCTTCGAGTTCCTACTCGAGGCACTGCGTGAGCTCGGTGTCGAGTACGATGTGGAACGGGACGACAAGGGGAACAT
CGTGTACGTAGAAACCGACGATTTCGTGGTCGAGTACGAGCGTCCGTCCGAGGCTTCCGAGATCGAGTGCGATCTGATGG
TAGTCGACGAGGCCGCTTCGATTCACGTACCCATCCTGGAGCGGATCCTGGATAACAACGACAAGGTGGTCTACTCCTCG
ACAATTCACGGTTACGAGGGTGCGGGCCGCGGGTTCTCGGTGAGGTTCCTCCAGAACGTTCGCAAGAGACGGGATGTTCG
CCTAATAGAGTTCAAGATGCACGAGCCGATCCGCTACGATTCGGATGACCCCATCGAACGATGGCTGTTCGACACGCTTC
TCCTGGACGCCGAGCCCGCGGACCTCGACAAGGAGGACCTGGAATGCGTGAAGGAAATGAGGGTCGAGTTCGAGAAGCCG
GACCTGAGGTACTGGTTCGAGGACCCTGAGGGTGAGGAGGAACTCCGTCAGTTCATCGGAATCTACGTGATGGCCCACTA
CCGCAACCGACCCAGCGACGTGATGGTACTCGCCGACGCTCCACATCACGAGGCCTACGCCCTCAAGACGGAGACCGGCA
AGATCGTCACCGCGCTCCAGGTAGCCCGTGAGGGCACTATACCCAGAGACGTGATCACCAAGATGCGTCGCGGTTACCGA
CCGCCGGGTAACGTGATCCCGGATCTGATGGTTCAACATCACGACGCGCTGGATTTCCCACGAATGAAGGGGCTTAGAAT
TGTAAGGATAGCGACACATCCCGACATCATGAGGCACGGTCTTGGCTCTCGAGCTCTGAAGGAGCTGGCAAAAATAGCGA
AAAAGAAGGACTACGACTGGATAGGAACAGGATTTGGAGCCAACGAAGAACTTACCCGATTCTGGTTGCGTAATGGTTTC
GTGCCCGTGCACATCAGCCCTAACCGCAACCCTGTGAGCGGAGAGTACTCCGTCGCCGTGATCCGGCCGATCTCCGAGGA
AGCCGAGGAGATCATTAACAGGGCGAACTTCGAGTTCCGGATCAAGCTGGCGGACTGGCTGGGCGAAACACATCGGGACC
TTGAGCCCGAGGTAGCCCGACTGCTCTTCGAACCCATGTCATCCTTGAGGTACCGGCCGACGCTGACCGAGGGCCAGCTC
AGACGGCTCAAAAAGTACGCGGATATGGTCCATACGTACGAGATCGCCGCCGACGCGGTGCGGGAGCTGGCGAAGGCGTA
CTTCCTGGACACCGAGGACAGGCCGGAACTATCCGAGGAGGAGGAACTGCTGCTCATCACCAAGTGTCTGCAGCGCTGGA
AGTGGGCGGATGTCGCGGACGTCCTGGGTGAGGAAGTACCCGACCTCATGCGATCCCTCAGGGACCTGGTGGGTCTTCTG
TATGAGGAGTACAAGGAGGATCTACAACGGAGCGCCGCGGTCGAGGGTATCCGGAAGGCGGTGGAGCGGCTGGCTGACAA
GGGGCTCACGGGTACCGTCATCGTGGAGGTCGAAGAAGGGGAGCCGAAGGAAGTCATCATACGTCGGGAGGAGCGGTTAG
AGCTCTGA

Upstream 100 bases:

>100_bases
TCGGCCCGAGGTCCGTCTTCATCCCCGAGTGGGGTGTACCATCTCGGGCTTTAAGTCGAATCTCACCGTCAAGGGAATAG
TGCGGCGGAGGGGGCGCGGA

Downstream 100 bases:

>100_bases
CGGAGACCGACAGCTCCCGCTTCGGCAACGCGGCCTTCGGCGGCTCACCGAAGACGTGGGCTTCGCCCTCGAGCACCGCC
AGTCCCACCGCGCAGCATAT

Product: P-loop ATPase fused to an acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 855; Mature: 854

Protein sequence:

>855_residues
MPDKAEVFFDEGVLDFADVDEIVLDVGEEALAEALAHRHRRMIVFQGDEGKAEAAGVVTAGAADVLFDVRDRPISVLYVT
DSLKEDTYARERYEEFRRVLEGFAEEANFEYELEALTFSGSKRALGTTWDLMVIDLSYDLDPDAIGRLVETVRGGGLVIF
QTPPFDRWRNMWTAFHKSLVTPPYTLDHVGKRFNRRFIRKLKEHDGVWIVDTDEWTAEPEPSEDVDLEVEVKRRERPDLD
PPDDAVLPEELYRMCATEDQFRALIRFEELLESNGKTALILTADRGRGKSALLGIAVAGAGVTTDVYDVVVTASEPENVA
VLFEFLLEALRELGVEYDVERDDKGNIVYVETDDFVVEYERPSEASEIECDLMVVDEAASIHVPILERILDNNDKVVYSS
TIHGYEGAGRGFSVRFLQNVRKRRDVRLIEFKMHEPIRYDSDDPIERWLFDTLLLDAEPADLDKEDLECVKEMRVEFEKP
DLRYWFEDPEGEEELRQFIGIYVMAHYRNRPSDVMVLADAPHHEAYALKTETGKIVTALQVAREGTIPRDVITKMRRGYR
PPGNVIPDLMVQHHDALDFPRMKGLRIVRIATHPDIMRHGLGSRALKELAKIAKKKDYDWIGTGFGANEELTRFWLRNGF
VPVHISPNRNPVSGEYSVAVIRPISEEAEEIINRANFEFRIKLADWLGETHRDLEPEVARLLFEPMSSLRYRPTLTEGQL
RRLKKYADMVHTYEIAADAVRELAKAYFLDTEDRPELSEEEELLLITKCLQRWKWADVADVLGEEVPDLMRSLRDLVGLL
YEEYKEDLQRSAAVEGIRKAVERLADKGLTGTVIVEVEEGEPKEVIIRREERLEL

Sequences:

>Translated_855_residues
MPDKAEVFFDEGVLDFADVDEIVLDVGEEALAEALAHRHRRMIVFQGDEGKAEAAGVVTAGAADVLFDVRDRPISVLYVT
DSLKEDTYARERYEEFRRVLEGFAEEANFEYELEALTFSGSKRALGTTWDLMVIDLSYDLDPDAIGRLVETVRGGGLVIF
QTPPFDRWRNMWTAFHKSLVTPPYTLDHVGKRFNRRFIRKLKEHDGVWIVDTDEWTAEPEPSEDVDLEVEVKRRERPDLD
PPDDAVLPEELYRMCATEDQFRALIRFEELLESNGKTALILTADRGRGKSALLGIAVAGAGVTTDVYDVVVTASEPENVA
VLFEFLLEALRELGVEYDVERDDKGNIVYVETDDFVVEYERPSEASEIECDLMVVDEAASIHVPILERILDNNDKVVYSS
TIHGYEGAGRGFSVRFLQNVRKRRDVRLIEFKMHEPIRYDSDDPIERWLFDTLLLDAEPADLDKEDLECVKEMRVEFEKP
DLRYWFEDPEGEEELRQFIGIYVMAHYRNRPSDVMVLADAPHHEAYALKTETGKIVTALQVAREGTIPRDVITKMRRGYR
PPGNVIPDLMVQHHDALDFPRMKGLRIVRIATHPDIMRHGLGSRALKELAKIAKKKDYDWIGTGFGANEELTRFWLRNGF
VPVHISPNRNPVSGEYSVAVIRPISEEAEEIINRANFEFRIKLADWLGETHRDLEPEVARLLFEPMSSLRYRPTLTEGQL
RRLKKYADMVHTYEIAADAVRELAKAYFLDTEDRPELSEEEELLLITKCLQRWKWADVADVLGEEVPDLMRSLRDLVGLL
YEEYKEDLQRSAAVEGIRKAVERLADKGLTGTVIVEVEEGEPKEVIIRREERLEL
>Mature_854_residues
PDKAEVFFDEGVLDFADVDEIVLDVGEEALAEALAHRHRRMIVFQGDEGKAEAAGVVTAGAADVLFDVRDRPISVLYVTD
SLKEDTYARERYEEFRRVLEGFAEEANFEYELEALTFSGSKRALGTTWDLMVIDLSYDLDPDAIGRLVETVRGGGLVIFQ
TPPFDRWRNMWTAFHKSLVTPPYTLDHVGKRFNRRFIRKLKEHDGVWIVDTDEWTAEPEPSEDVDLEVEVKRRERPDLDP
PDDAVLPEELYRMCATEDQFRALIRFEELLESNGKTALILTADRGRGKSALLGIAVAGAGVTTDVYDVVVTASEPENVAV
LFEFLLEALRELGVEYDVERDDKGNIVYVETDDFVVEYERPSEASEIECDLMVVDEAASIHVPILERILDNNDKVVYSST
IHGYEGAGRGFSVRFLQNVRKRRDVRLIEFKMHEPIRYDSDDPIERWLFDTLLLDAEPADLDKEDLECVKEMRVEFEKPD
LRYWFEDPEGEEELRQFIGIYVMAHYRNRPSDVMVLADAPHHEAYALKTETGKIVTALQVAREGTIPRDVITKMRRGYRP
PGNVIPDLMVQHHDALDFPRMKGLRIVRIATHPDIMRHGLGSRALKELAKIAKKKDYDWIGTGFGANEELTRFWLRNGFV
PVHISPNRNPVSGEYSVAVIRPISEEAEEIINRANFEFRIKLADWLGETHRDLEPEVARLLFEPMSSLRYRPTLTEGQLR
RLKKYADMVHTYEIAADAVRELAKAYFLDTEDRPELSEEEELLLITKCLQRWKWADVADVLGEEVPDLMRSLRDLVGLLY
EEYKEDLQRSAAVEGIRKAVERLADKGLTGTVIVEVEEGEPKEVIIRREERLEL

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP)

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain

Homologues:

Organism=Homo sapiens, GI221316723, Length=876, Percent_Identity=26.027397260274, Blast_Score=221, Evalue=3e-57,
Organism=Homo sapiens, GI221316741, Length=793, Percent_Identity=26.2295081967213, Blast_Score=214, Evalue=3e-55,
Organism=Escherichia coli, GI1788818, Length=583, Percent_Identity=25.2144082332762, Blast_Score=168, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17507621, Length=543, Percent_Identity=30.2025782688766, Blast_Score=201, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6324197, Length=839, Percent_Identity=25.6257449344458, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24640662, Length=773, Percent_Identity=27.4256144890039, Blast_Score=267, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TMCA_METKA (Q8TYZ5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613433.1
- ProteinModelPortal:   Q8TYZ5
- GeneID:   1477449
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0146
- NMPDR:   fig|190192.1.peg.146
- HOGENOM:   HBG553581
- OMA:   GNIIPDR
- ProtClustDB:   CLSK862705
- BioCyc:   MKAN190192:MK0146-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01886
- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR013562
- InterPro:   IPR007807
- Gene3D:   G3DSA:3.40.630.30

Pfam domain/function: PF00583 Acetyltransf_1; PF08351 DUF1726; PF05127 DUF699; SSF55729 Acyl_CoA_acyltransferase

EC number: NA

Molecular weight: Translated: 98137; Mature: 98006

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS51186 GNAT

Important sites: BINDING 260-260 BINDING 438-438 BINDING 630-630 BINDING 637-637

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDKAEVFFDEGVLDFADVDEIVLDVGEEALAEALAHRHRRMIVFQGDEGKAEAAGVVTA
CCCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEC
GAADVLFDVRDRPISVLYVTDSLKEDTYARERYEEFRRVLEGFAEEANFEYELEALTFSG
CHHHHHEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
SKRALGTTWDLMVIDLSYDLDPDAIGRLVETVRGGGLVIFQTPPFDRWRNMWTAFHKSLV
CCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHC
TPPYTLDHVGKRFNRRFIRKLKEHDGVWIVDTDEWTAEPEPSEDVDLEVEVKRRERPDLD
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEHHCCCCCCC
PPDDAVLPEELYRMCATEDQFRALIRFEELLESNGKTALILTADRGRGKSALLGIAVAGA
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHEEEEEEECC
GVTTDVYDVVVTASEPENVAVLFEFLLEALRELGVEYDVERDDKGNIVYVETDDFVVEYE
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCCEEEEEC
RPSEASEIECDLMVVDEAASIHVPILERILDNNDKVVYSSTIHGYEGAGRGFSVRFLQNV
CCCCCCCCEEEEEEECCCCCEEHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEHHHHHHH
RKRRDVRLIEFKMHEPIRYDSDDPIERWLFDTLLLDAEPADLDKEDLECVKEMRVEFEKP
HHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
DLRYWFEDPEGEEELRQFIGIYVMAHYRNRPSDVMVLADAPHHEAYALKTETGKIVTALQ
CCEEECCCCCCHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHH
VAREGTIPRDVITKMRRGYRPPGNVIPDLMVQHHDALDFPRMKGLRIVRIATHPDIMRHG
HHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHC
LGSRALKELAKIAKKKDYDWIGTGFGANEELTRFWLRNGFVPVHISPNRNPVSGEYSVAV
CCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEE
IRPISEEAEEIINRANFEFRIKLADWLGETHRDLEPEVARLLFEPMSSLRYRPTLTEGQL
EECCHHHHHHHHHHCCCEEEEEEHHHCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
RRLKKYADMVHTYEIAADAVRELAKAYFLDTEDRPELSEEEELLLITKCLQRWKWADVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHH
VLGEEVPDLMRSLRDLVGLLYEEYKEDLQRSAAVEGIRKAVERLADKGLTGTVIVEVEEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
EPKEVIIRREERLEL
CCHHHEEECHHHCCC
>Mature Secondary Structure 
PDKAEVFFDEGVLDFADVDEIVLDVGEEALAEALAHRHRRMIVFQGDEGKAEAAGVVTA
CCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEC
GAADVLFDVRDRPISVLYVTDSLKEDTYARERYEEFRRVLEGFAEEANFEYELEALTFSG
CHHHHHEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
SKRALGTTWDLMVIDLSYDLDPDAIGRLVETVRGGGLVIFQTPPFDRWRNMWTAFHKSLV
CCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHC
TPPYTLDHVGKRFNRRFIRKLKEHDGVWIVDTDEWTAEPEPSEDVDLEVEVKRRERPDLD
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEHHCCCCCCC
PPDDAVLPEELYRMCATEDQFRALIRFEELLESNGKTALILTADRGRGKSALLGIAVAGA
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHEEEEEEECC
GVTTDVYDVVVTASEPENVAVLFEFLLEALRELGVEYDVERDDKGNIVYVETDDFVVEYE
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCCEEEEEC
RPSEASEIECDLMVVDEAASIHVPILERILDNNDKVVYSSTIHGYEGAGRGFSVRFLQNV
CCCCCCCCEEEEEEECCCCCEEHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEHHHHHHH
RKRRDVRLIEFKMHEPIRYDSDDPIERWLFDTLLLDAEPADLDKEDLECVKEMRVEFEKP
HHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
DLRYWFEDPEGEEELRQFIGIYVMAHYRNRPSDVMVLADAPHHEAYALKTETGKIVTALQ
CCEEECCCCCCHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHH
VAREGTIPRDVITKMRRGYRPPGNVIPDLMVQHHDALDFPRMKGLRIVRIATHPDIMRHG
HHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHC
LGSRALKELAKIAKKKDYDWIGTGFGANEELTRFWLRNGFVPVHISPNRNPVSGEYSVAV
CCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEE
IRPISEEAEEIINRANFEFRIKLADWLGETHRDLEPEVARLLFEPMSSLRYRPTLTEGQL
EECCHHHHHHHHHHCCCEEEEEEHHHCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
RRLKKYADMVHTYEIAADAVRELAKAYFLDTEDRPELSEEEELLLITKCLQRWKWADVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHH
VLGEEVPDLMRSLRDLVGLLYEEYKEDLQRSAAVEGIRKAVERLADKGLTGTVIVEVEEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
EPKEVIIRREERLEL
CCHHHEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014