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The map label for this gene is glyA

Identifier: 20093562

GI number: 20093562

Start: 115928

End: 117214

Strand: Reverse

Name: glyA

Synonym: MK0122

Alternate gene names: 20093562

Gene position: 117214-115928 (Counterclockwise)

Preceding gene: 20093567

Following gene: 20093561

Centisome position: 6.92

GC content: 63.4

Gene sequence:

>1287_bases
GTGTTCGGGTTGGAGGACGTACATTCCGTCGTGCGGGCGGTGGAGAAGCATCACGAGTGGCTGAAGAAGTGCCTCCCCAT
GATCGCCAGCGAGAACGTGACCAGCCCCGCGGTGCGCGAGATGCTCGTGACGGATTTCGGGCACCGGTACGCCGAAGGGA
AACCCGGTGAGCGGCTTTACGAGGGTTGTGAGTACATCGACGAAGTCGAGCTGGCCTGTGTACGACTGGCCAAAGAGCTC
TTCGGTGCCGAGCACGCGAACGTCCAGCCCACATCCGGCGTGGTGGCCAACCTAGCCGCTCTCTTCGCGTTGACCGAGCC
CGGTGACACGATCTTGGGGCTTCGGATCTCCCACGGGGGTCACATCAGCCACCACGATATCAGCGCTCCGGGTGTTAGAG
GGCTCAACGTCGAGTACCTACCGTTCGACGAGGAGGATATGGCCATCGACGTCGACGGTATGGTCCGCAAGATCGAGGAA
GTCGAGCCGTCGGTGGTCATGCTCGGGGCTAGCCTGTTCCTGTTTCCACATCCGGTGGAGGAGGCCGTAGAAGCCGTGGA
GGCCGTCGGAGGTTACGTGGTGTACGACGCCGCCCACGTGCTCGGACTGATCGCCGGCGGTCAGTTCCAGGACCCTATCC
GAGAAGGCGCTCACGTGGTCACCGGAAGCACTCACAAGACGTTTCCGGGTCCTCAGGGCGGAATCGTGCTCTGTCAACGG
GATCTAGCCGACGACATCGACGAGGCAGTGTTTCCGGGGCTCGTGAGCAACCACCACCTGCACCACGTGGCGGCGCTGGC
CGTAGCGCTCGCGGAGTTCAAGGAGTACGGGGAGCGATACGCGCGTGACACGGTTCGGAACGCTAAGGCTCTGGCCGAGG
CTCTGTACGCCGAGGGGCTCCGGGTACTCTGTGAGCACCGGGGCTTCACGGAGTCCCATCAAATCGCCGTGGACGTGCGG
GAGCAAGGAGGGGGAGCGGTCATCGCCGAAAAACTGGAGAGTGCCAACATCCTGTGCAACAAGAACCTGCTACCCTGGGA
CGACGAGTCGAAATCCCACGATCCGAGCGGTATCCGGCTGGGCACCCAGGAGCTCACCCGGTTGGGCATGGGTCTTTCTG
AGATGGAGTACATCGCCGAGCTGATCGCGGACGTCGTGCTCGGTCGCAGGGAGCCGTCCGAGGTCCGGAAGGACGTCGAG
GAACTCCGGCGTGAGTTCCAGGAGGTCAAGTACGGGTTCGGGTCGGGAGTGGGTGCCCACGAATGGCCCAGGCTCGCCGA
CTGGTAG

Upstream 100 bases:

>100_bases
GAGCCTGGCTTTACGCTCACCTCGACGTGCGTGAGCACACCTTTGGTCACTGCCTCGGCCAGTGCTCGCGCCTCGACCAA
AGTCAAAACCCTCCCTCGGG

Downstream 100 bases:

>100_bases
TGTTAGACTTCGATGGAACGCTGGTCGATGGGGAGACCATCGATCTAATCGCGGAGGCGGCCGGAGTCGACGATGAAGTC
GAAGAGATCACGAGACGGGC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MFGLEDVHSVVRAVEKHHEWLKKCLPMIASENVTSPAVREMLVTDFGHRYAEGKPGERLYEGCEYIDEVELACVRLAKEL
FGAEHANVQPTSGVVANLAALFALTEPGDTILGLRISHGGHISHHDISAPGVRGLNVEYLPFDEEDMAIDVDGMVRKIEE
VEPSVVMLGASLFLFPHPVEEAVEAVEAVGGYVVYDAAHVLGLIAGGQFQDPIREGAHVVTGSTHKTFPGPQGGIVLCQR
DLADDIDEAVFPGLVSNHHLHHVAALAVALAEFKEYGERYARDTVRNAKALAEALYAEGLRVLCEHRGFTESHQIAVDVR
EQGGGAVIAEKLESANILCNKNLLPWDDESKSHDPSGIRLGTQELTRLGMGLSEMEYIAELIADVVLGRREPSEVRKDVE
ELRREFQEVKYGFGSGVGAHEWPRLADW

Sequences:

>Translated_428_residues
MFGLEDVHSVVRAVEKHHEWLKKCLPMIASENVTSPAVREMLVTDFGHRYAEGKPGERLYEGCEYIDEVELACVRLAKEL
FGAEHANVQPTSGVVANLAALFALTEPGDTILGLRISHGGHISHHDISAPGVRGLNVEYLPFDEEDMAIDVDGMVRKIEE
VEPSVVMLGASLFLFPHPVEEAVEAVEAVGGYVVYDAAHVLGLIAGGQFQDPIREGAHVVTGSTHKTFPGPQGGIVLCQR
DLADDIDEAVFPGLVSNHHLHHVAALAVALAEFKEYGERYARDTVRNAKALAEALYAEGLRVLCEHRGFTESHQIAVDVR
EQGGGAVIAEKLESANILCNKNLLPWDDESKSHDPSGIRLGTQELTRLGMGLSEMEYIAELIADVVLGRREPSEVRKDVE
ELRREFQEVKYGFGSGVGAHEWPRLADW
>Mature_428_residues
MFGLEDVHSVVRAVEKHHEWLKKCLPMIASENVTSPAVREMLVTDFGHRYAEGKPGERLYEGCEYIDEVELACVRLAKEL
FGAEHANVQPTSGVVANLAALFALTEPGDTILGLRISHGGHISHHDISAPGVRGLNVEYLPFDEEDMAIDVDGMVRKIEE
VEPSVVMLGASLFLFPHPVEEAVEAVEAVGGYVVYDAAHVLGLIAGGQFQDPIREGAHVVTGSTHKTFPGPQGGIVLCQR
DLADDIDEAVFPGLVSNHHLHHVAALAVALAEFKEYGERYARDTVRNAKALAEALYAEGLRVLCEHRGFTESHQIAVDVR
EQGGGAVIAEKLESANILCNKNLLPWDDESKSHDPSGIRLGTQELTRLGMGLSEMEYIAELIADVVLGRREPSEVRKDVE
ELRREFQEVKYGFGSGVGAHEWPRLADW

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=377, Percent_Identity=33.42175066313, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI261862352, Length=377, Percent_Identity=33.42175066313, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI261862350, Length=377, Percent_Identity=33.42175066313, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI261862348, Length=377, Percent_Identity=33.42175066313, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI261862346, Length=372, Percent_Identity=33.6021505376344, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI22547186, Length=435, Percent_Identity=32.4137931034483, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI22547189, Length=415, Percent_Identity=30.8433734939759, Blast_Score=152, Evalue=8e-37,
Organism=Escherichia coli, GI1788902, Length=385, Percent_Identity=37.6623376623377, Blast_Score=203, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI25144732, Length=426, Percent_Identity=33.3333333333333, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI25144729, Length=426, Percent_Identity=33.3333333333333, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319739, Length=430, Percent_Identity=31.8604651162791, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6323087, Length=445, Percent_Identity=32.8089887640449, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24640005, Length=398, Percent_Identity=35.427135678392, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI221329721, Length=448, Percent_Identity=33.2589285714286, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_METKA (Q8TZ19)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613409.1
- ProteinModelPortal:   Q8TZ19
- GeneID:   1477425
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0122
- NMPDR:   fig|190192.1.peg.122
- HOGENOM:   HBG301263
- OMA:   SGIVTNR
- ProtClustDB:   PRK00011
- BioCyc:   MKAN190192:MK0122-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_A
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46898; Mature: 46898

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGLEDVHSVVRAVEKHHEWLKKCLPMIASENVTSPAVREMLVTDFGHRYAEGKPGERLY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
EGCEYIDEVELACVRLAKELFGAEHANVQPTSGVVANLAALFALTEPGDTILGLRISHGG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCC
HISHHDISAPGVRGLNVEYLPFDEEDMAIDVDGMVRKIEEVEPSVVMLGASLFLFPHPVE
CCCCCCCCCCCCCCCCEEECCCCCCCCEECHHHHHHHHHHCCCCEEEECHHHHCCCCCHH
EAVEAVEAVGGYVVYDAAHVLGLIAGGQFQDPIREGAHVVTGSTHKTFPGPQGGIVLCQR
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCCEEEECCCCCCCCCCCCCEEEECH
DLADDIDEAVFPGLVSNHHLHHVAALAVALAEFKEYGERYARDTVRNAKALAEALYAEGL
HHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLCEHRGFTESHQIAVDVREQGGGAVIAEKLESANILCNKNLLPWDDESKSHDPSGIRL
HHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEE
GTQELTRLGMGLSEMEYIAELIADVVLGRREPSEVRKDVEELRREFQEVKYGFGSGVGAH
CHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EWPRLADW
CCCCCCCC
>Mature Secondary Structure
MFGLEDVHSVVRAVEKHHEWLKKCLPMIASENVTSPAVREMLVTDFGHRYAEGKPGERLY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
EGCEYIDEVELACVRLAKELFGAEHANVQPTSGVVANLAALFALTEPGDTILGLRISHGG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCC
HISHHDISAPGVRGLNVEYLPFDEEDMAIDVDGMVRKIEEVEPSVVMLGASLFLFPHPVE
CCCCCCCCCCCCCCCCEEECCCCCCCCEECHHHHHHHHHHCCCCEEEECHHHHCCCCCHH
EAVEAVEAVGGYVVYDAAHVLGLIAGGQFQDPIREGAHVVTGSTHKTFPGPQGGIVLCQR
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCCEEEECCCCCCCCCCCCCEEEECH
DLADDIDEAVFPGLVSNHHLHHVAALAVALAEFKEYGERYARDTVRNAKALAEALYAEGL
HHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLCEHRGFTESHQIAVDVREQGGGAVIAEKLESANILCNKNLLPWDDESKSHDPSGIRL
HHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEE
GTQELTRLGMGLSEMEYIAELIADVVLGRREPSEVRKDVEELRREFQEVKYGFGSGVGAH
CHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EWPRLADW
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014