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The map label for this gene is rfcL

Identifier: 20093445

GI number: 20093445

Start: 3811

End: 5343

Strand: Reverse

Name: rfcL

Synonym: MK0005

Alternate gene names: 20093445

Gene position: 5343-3811 (Counterclockwise)

Preceding gene: 20093446

Following gene: 20093443

Centisome position: 0.32

GC content: 60.01

Gene sequence:

>1533_bases
TTGGTCCCTTGGGTTGAGAAGTACCGACCTAGGTCGCTCAAAGAGCTGGTGAACCAGGACGAGGCCAAGAAGGAGCTAGC
CGCATGGGCGAACGAGTGGGCGCGCGGTAGTATTCCGGAACCGCGGGCCGTGCTACTTCATGGACCACCCGGGACCGGAA
AGACGAGCGCCGCGTACGCCCTAGCCCACGACTTCGGCTGGGACGTGATCGAACTCAACGCGTCGGACAAGCGCACACGA
AACGTGATCGAGAAGATCGTGGGCGGCGCCTCCACCTCACGCTCCCTCCTCCGAATGACCAGGGAAGCCGGGGGAGATTA
CGAACATGTGGAGGGTCACTCGGACAGGGTTCTAGTCCTCGTGGACGAGGTCGACGGGATCGATCCCAGGGAAGATAGAG
GTGGGGTGACCGCGCTCACGCGGGCCGTACGTCAGGCCCGTAACCCCATGGTCCTCGTCGCCAACGACCCGTGGGTGTTA
CCCAAGTCCCTACGTGACGCCGTTCGGATGATCGAGTTCCGCCGGCTCCGCGTCAACGACATCGTCGAGGCATTACGTCG
CATCTGCGAGCGGGAGGGTATCGAGTACGAGGAGGTGGCCCTGCGTCGGATCGCGAAGCGTGCCAGGGGAGATCTTAGGG
CTGCGATCAACGATCTGGAGGCGCTCGCAAGGCCCACGGGTAGGGTAACGTCCGATGACGTGGAAGCGCTCGGGTGGCGT
GATAAGGAGATCACCATCTTCGAGGCTCTGGGTAGGATCTTCAACAAACCGCCCAGACAGGCCAGACGCGCGCTCTGGAA
CTTAGACGAGGATCCGGACGACGTGATCCTGTGGATAGCTCAGAACATACCTCGAGCGTACCGAGATCCGGAGGAGATCG
CCCGCGCGTATGATTACCTGTCCAAAGCCGACGTATTCTCGAGTCGTGCGATAGAGACCGGGGACTGGAGGTTCAAGTAC
GTCTACGCCACCGACCTCATGACGTCGGGTGTAGCCGCGGCCCGGAAGGGTAAACCACCGGGGTTCGTTCGGTTCCAACC
TCCCAAGATCCTGCGGAAGCTGGGGACTACCCGCAAAGAGCGTGAGGTCCGGAATTCCATAGCGAAAAAGATCGCGGAAC
GCATGCATGTGTCCACACGCCGTGCCAAGATGGACGTGATTTCAGTGCTCGAGATCGCGTTCCGGAAGGTCGCGGACAAT
CCCACCGATCGCGGTTTGGAAATCCTAGGAGGGATCGCCGGCTATCTAGAACTCTCCAAACGGGAAATCGGGTTCCTCTG
CGGCGATCCACAGGTGGCTCAGCGGGTATATCAGCGGGCGTTGCGCGTTCGCGAGAAGCTGCGGAAGATCCGCAGAGAGC
GGGTGAAAGGTGCGATGGAGTCCATGCTCGAGAGAAAACGTGAAGAATCGGAGGTAGAAGAGGAAGCGAAGGAGATCGAG
GAAGCCGTAGAAAAAGCGGAGGAAGAAGAGGAGAGAGAGGAAAAGAAGAAGGAAGGAGGAGGTGAACAGAGGACGTTAGA
CGCGTTCTTCTGA

Upstream 100 bases:

>100_bases
AGTACCGCCTGATCCGCGGCTCGGACGAGCGGATCCAGTTAGAGGCCCTGTTAGCCCGAATTCACGCACTCGGTAACGAG
TACTCCGGCGGGTAGCACCG

Downstream 100 bases:

>100_bases
GGTTTACCCGAACAGTGCACCGAGGCCCGCTGCCGCTTCCTCTTCCGCTTCTTCTTCCTCCTCTTCCTCTTCTTCCTCTT
CCGCTTCGGCCTCTTCCTCT

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MVPWVEKYRPRSLKELVNQDEAKKELAAWANEWARGSIPEPRAVLLHGPPGTGKTSAAYALAHDFGWDVIELNASDKRTR
NVIEKIVGGASTSRSLLRMTREAGGDYEHVEGHSDRVLVLVDEVDGIDPREDRGGVTALTRAVRQARNPMVLVANDPWVL
PKSLRDAVRMIEFRRLRVNDIVEALRRICEREGIEYEEVALRRIAKRARGDLRAAINDLEALARPTGRVTSDDVEALGWR
DKEITIFEALGRIFNKPPRQARRALWNLDEDPDDVILWIAQNIPRAYRDPEEIARAYDYLSKADVFSSRAIETGDWRFKY
VYATDLMTSGVAAARKGKPPGFVRFQPPKILRKLGTTRKEREVRNSIAKKIAERMHVSTRRAKMDVISVLEIAFRKVADN
PTDRGLEILGGIAGYLELSKREIGFLCGDPQVAQRVYQRALRVREKLRKIRRERVKGAMESMLERKREESEVEEEAKEIE
EAVEKAEEEEEREEKKKEGGGEQRTLDAFF

Sequences:

>Translated_510_residues
MVPWVEKYRPRSLKELVNQDEAKKELAAWANEWARGSIPEPRAVLLHGPPGTGKTSAAYALAHDFGWDVIELNASDKRTR
NVIEKIVGGASTSRSLLRMTREAGGDYEHVEGHSDRVLVLVDEVDGIDPREDRGGVTALTRAVRQARNPMVLVANDPWVL
PKSLRDAVRMIEFRRLRVNDIVEALRRICEREGIEYEEVALRRIAKRARGDLRAAINDLEALARPTGRVTSDDVEALGWR
DKEITIFEALGRIFNKPPRQARRALWNLDEDPDDVILWIAQNIPRAYRDPEEIARAYDYLSKADVFSSRAIETGDWRFKY
VYATDLMTSGVAAARKGKPPGFVRFQPPKILRKLGTTRKEREVRNSIAKKIAERMHVSTRRAKMDVISVLEIAFRKVADN
PTDRGLEILGGIAGYLELSKREIGFLCGDPQVAQRVYQRALRVREKLRKIRRERVKGAMESMLERKREESEVEEEAKEIE
EAVEKAEEEEEREEKKKEGGGEQRTLDAFF
>Mature_510_residues
MVPWVEKYRPRSLKELVNQDEAKKELAAWANEWARGSIPEPRAVLLHGPPGTGKTSAAYALAHDFGWDVIELNASDKRTR
NVIEKIVGGASTSRSLLRMTREAGGDYEHVEGHSDRVLVLVDEVDGIDPREDRGGVTALTRAVRQARNPMVLVANDPWVL
PKSLRDAVRMIEFRRLRVNDIVEALRRICEREGIEYEEVALRRIAKRARGDLRAAINDLEALARPTGRVTSDDVEALGWR
DKEITIFEALGRIFNKPPRQARRALWNLDEDPDDVILWIAQNIPRAYRDPEEIARAYDYLSKADVFSSRAIETGDWRFKY
VYATDLMTSGVAAARKGKPPGFVRFQPPKILRKLGTTRKEREVRNSIAKKIAERMHVSTRRAKMDVISVLEIAFRKVADN
PTDRGLEILGGIAGYLELSKREIGFLCGDPQVAQRVYQRALRVREKLRKIRRERVKGAMESMLERKREESEVEEEAKEIE
EAVEKAEEEEEREEKKKEGGGEQRTLDAFF

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily

Homologues:

Organism=Homo sapiens, GI32528306, Length=435, Percent_Identity=25.2873563218391, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI31563534, Length=287, Percent_Identity=26.4808362369338, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI6677723, Length=323, Percent_Identity=24.7678018575851, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI194306571, Length=321, Percent_Identity=24.2990654205607, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI194306567, Length=321, Percent_Identity=24.2990654205607, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI4506491, Length=235, Percent_Identity=28.936170212766, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI31881687, Length=235, Percent_Identity=28.936170212766, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI27501458, Length=266, Percent_Identity=24.812030075188, Blast_Score=73, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17563226, Length=428, Percent_Identity=23.3644859813084, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17541988, Length=241, Percent_Identity=25.3112033195021, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17554730, Length=231, Percent_Identity=27.7056277056277, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71986063, Length=314, Percent_Identity=23.5668789808917, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17541368, Length=251, Percent_Identity=24.3027888446215, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324791, Length=234, Percent_Identity=31.6239316239316, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324478, Length=240, Percent_Identity=27.5, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323724, Length=454, Percent_Identity=22.6872246696035, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322528, Length=249, Percent_Identity=28.5140562248996, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324039, Length=244, Percent_Identity=27.8688524590164, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17737765, Length=243, Percent_Identity=28.3950617283951, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI62472391, Length=265, Percent_Identity=26.7924528301887, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI18859927, Length=231, Percent_Identity=30.3030303030303, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17647857, Length=285, Percent_Identity=24.9122807017544, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921076, Length=241, Percent_Identity=25.7261410788382, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCL_METKA (Q8TZC5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613292.1
- ProteinModelPortal:   Q8TZC5
- SMR:   Q8TZC5
- GeneID:   1477307
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0005
- NMPDR:   fig|190192.1.peg.5
- HOGENOM:   HBG541322
- OMA:   ELNASDK
- ProtClustDB:   PRK04195
- BioCyc:   MKAN190192:MK0005-MONOMER
- HAMAP:   MF_01508
- InterPro:   IPR003593
- InterPro:   IPR003959
- SMART:   SM00382

Pfam domain/function: PF00004 AAA

EC number: NA

Molecular weight: Translated: 58256; Mature: 58256

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPWVEKYRPRSLKELVNQDEAKKELAAWANEWARGSIPEPRAVLLHGPPGTGKTSAAYA
CCCCHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHH
LAHDFGWDVIELNASDKRTRNVIEKIVGGASTSRSLLRMTREAGGDYEHVEGHSDRVLVL
HHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
VDEVDGIDPREDRGGVTALTRAVRQARNPMVLVANDPWVLPKSLRDAVRMIEFRRLRVND
EECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
IVEALRRICEREGIEYEEVALRRIAKRARGDLRAAINDLEALARPTGRVTSDDVEALGWR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCC
DKEITIFEALGRIFNKPPRQARRALWNLDEDPDDVILWIAQNIPRAYRDPEEIARAYDYL
CCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEHHHHHCCHHCCCHHHHHHHHHHH
SKADVFSSRAIETGDWRFKYVYATDLMTSGVAAARKGKPPGFVRFQPPKILRKLGTTRKE
HHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHCCCHHH
REVRNSIAKKIAERMHVSTRRAKMDVISVLEIAFRKVADNPTDRGLEILGGIAGYLELSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
REIGFLCGDPQVAQRVYQRALRVREKLRKIRRERVKGAMESMLERKREESEVEEEAKEIE
HHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAVEKAEEEEEREEKKKEGGGEQRTLDAFF
HHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
>Mature Secondary Structure
MVPWVEKYRPRSLKELVNQDEAKKELAAWANEWARGSIPEPRAVLLHGPPGTGKTSAAYA
CCCCHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHH
LAHDFGWDVIELNASDKRTRNVIEKIVGGASTSRSLLRMTREAGGDYEHVEGHSDRVLVL
HHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
VDEVDGIDPREDRGGVTALTRAVRQARNPMVLVANDPWVLPKSLRDAVRMIEFRRLRVND
EECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
IVEALRRICEREGIEYEEVALRRIAKRARGDLRAAINDLEALARPTGRVTSDDVEALGWR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCC
DKEITIFEALGRIFNKPPRQARRALWNLDEDPDDVILWIAQNIPRAYRDPEEIARAYDYL
CCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEHHHHHCCHHCCCHHHHHHHHHHH
SKADVFSSRAIETGDWRFKYVYATDLMTSGVAAARKGKPPGFVRFQPPKILRKLGTTRKE
HHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHCCCHHH
REVRNSIAKKIAERMHVSTRRAKMDVISVLEIAFRKVADNPTDRGLEILGGIAGYLELSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
REIGFLCGDPQVAQRVYQRALRVREKLRKIRRERVKGAMESMLERKREESEVEEEAKEIE
HHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAVEKAEEEEEREEKKKEGGGEQRTLDAFF
HHHHHHHHHHHHHHHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014