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The map label for this gene is trmE [C]

Identifier: 20093443

GI number: 20093443

Start: 2357

End: 3415

Strand: Reverse

Name: trmE [C]

Synonym: MK0003

Alternate gene names: 20093443

Gene position: 3415-2357 (Counterclockwise)

Preceding gene: 20093445

Following gene: 20093442

Centisome position: 0.2

GC content: 61.85

Gene sequence:

>1059_bases
GTGTCCGGGAACCCGTTTCGGAAGATGCCCGAGGTACCGGACCCGGAAGAGCTGATCGACGTCGCCTTCCGACGGGCGGA
GAGAGCCGCTGAAGGCACCAGGAAATCGTTCTACGGTACCAGGACACCGCCTGAAGTCAGGGCCAGGTCCATCGAGATAG
CACGCGTCAACACGGCGTGCCAGTTGGTGCAGGACCGCCTCTGGGAGATCGTGAGGAAGACGCCCAACCTCGACGAGCTT
CACCCGTTCTACCGGGAGTTGGCGGACGCGCTAGCGGGCATCGACAGGCTGAAAAGCAGTCTGGCCGACGTGCATACGGT
CGCCAAGATAGCCAGGTTGATCCGGGAGGAGTACACTCGGAAGATCAAGAGGGCGAGGGACCCCCGGACCGCCGCGGAGT
TGAGACGTCAGGCGTTCGGGAGACTGGCTTCGACGATCCGCAGGAAGGTTGGAGACGCGCTCAGGTTCCTCCGGAAGGTG
CAGCCGAAGCTCGTAGACCTACCCGCGATCGACACCGAGATGTTCACCGTGACCTTAGCGGGGTTCCCCAACGTCGGTAA
AACCACCCTTATGACGGTGCTCACGGGCTCGAGGCCGGAGATCGCCCCGTATCCCTTCACTACCAAGGGCATCCAAGTAG
GCTACATGGAGCGCCCGTACCCCGTGCAAATGCTCGACACACCGGGTTTGCTGGAACGACCGGAGGAAGAGCGCAATCCG
GTCGAGCGACAGGCGATCGCGGCCCTAAAGCACGTGACGGACGCCGTGCTCTTCCTGATAGATCCCACCGGAACCTGTGG
ATATCCAGTGGAGGAGCAGCTGGAGTTGCTGGACCGCGTCCGGAAGGAGTTCGACGTACCGGTCTACGTGGTGCTGACGA
AAGCCGACCTACGGGATCTGTGGGAGGAACCCGATCTCGAAGGGGAACCCGTTTATAAGGTCTCGGCGACGGAGAGGACC
GGACTGAAAGAACTCCGCGAGCTCCTGAACGATCTGGCGAGGGGGCACTACAGTGGGCGTGATCGAGGACATGATGAAGG
TCGGGATGAGGAGCGCTAA

Upstream 100 bases:

>100_bases
AGCAACGCCGCGTAAATATACTCCATTCGCGGAACCCCCGTCGCCGTTTTCTCGCCGGACGGCCTCTGTCGACTCAAAAA
GGTTTACGGGAGGCGATACC

Downstream 100 bases:

>100_bases
GGCCGGCCTCGAGGCCACGGAGGAGTTGATAAAGCTCTTCCGGGAGGACGGGCGGCTAGTAGGGTCTATCTTGAAGGAAA
TGGAGCCTGAGGAGATTACG

Product: GTPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSGNPFRKMPEVPDPEELIDVAFRRAERAAEGTRKSFYGTRTPPEVRARSIEIARVNTACQLVQDRLWEIVRKTPNLDEL
HPFYRELADALAGIDRLKSSLADVHTVAKIARLIREEYTRKIKRARDPRTAAELRRQAFGRLASTIRRKVGDALRFLRKV
QPKLVDLPAIDTEMFTVTLAGFPNVGKTTLMTVLTGSRPEIAPYPFTTKGIQVGYMERPYPVQMLDTPGLLERPEEERNP
VERQAIAALKHVTDAVLFLIDPTGTCGYPVEEQLELLDRVRKEFDVPVYVVLTKADLRDLWEEPDLEGEPVYKVSATERT
GLKELRELLNDLARGHYSGRDRGHDEGRDEER

Sequences:

>Translated_352_residues
MSGNPFRKMPEVPDPEELIDVAFRRAERAAEGTRKSFYGTRTPPEVRARSIEIARVNTACQLVQDRLWEIVRKTPNLDEL
HPFYRELADALAGIDRLKSSLADVHTVAKIARLIREEYTRKIKRARDPRTAAELRRQAFGRLASTIRRKVGDALRFLRKV
QPKLVDLPAIDTEMFTVTLAGFPNVGKTTLMTVLTGSRPEIAPYPFTTKGIQVGYMERPYPVQMLDTPGLLERPEEERNP
VERQAIAALKHVTDAVLFLIDPTGTCGYPVEEQLELLDRVRKEFDVPVYVVLTKADLRDLWEEPDLEGEPVYKVSATERT
GLKELRELLNDLARGHYSGRDRGHDEGRDEER
>Mature_351_residues
SGNPFRKMPEVPDPEELIDVAFRRAERAAEGTRKSFYGTRTPPEVRARSIEIARVNTACQLVQDRLWEIVRKTPNLDELH
PFYRELADALAGIDRLKSSLADVHTVAKIARLIREEYTRKIKRARDPRTAAELRRQAFGRLASTIRRKVGDALRFLRKVQ
PKLVDLPAIDTEMFTVTLAGFPNVGKTTLMTVLTGSRPEIAPYPFTTKGIQVGYMERPYPVQMLDTPGLLERPEEERNPV
ERQAIAALKHVTDAVLFLIDPTGTCGYPVEEQLELLDRVRKEFDVPVYVVLTKADLRDLWEEPDLEGEPVYKVSATERTG
LKELRELLNDLARGHYSGRDRGHDEGRDEER

Specific function: Exhibits A Very High Intrinsic Gtpase Hydrolysis Rate. Involved In The Biosynthesis Of The Hypermodified Nucleoside 5- Methylaminomethyl-2-Thiouridine, Which Is Found In The Wobble Position Of Some Trnas. [C]

COG id: COG1084

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasmic; may also be associated with the inner membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP1/OBG family. NOG subfamily [H]

Homologues:

Organism=Homo sapiens, GI55953087, Length=334, Percent_Identity=32.9341317365269, Blast_Score=186, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI25145602, Length=334, Percent_Identity=32.3353293413174, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI25145600, Length=334, Percent_Identity=32.3353293413174, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6325164, Length=304, Percent_Identity=32.2368421052632, Blast_Score=172, Evalue=8e-44,
Organism=Drosophila melanogaster, GI19921908, Length=339, Percent_Identity=31.858407079646, Blast_Score=183, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006073
- InterPro:   IPR010674
- InterPro:   IPR005225 [H]

Pfam domain/function: PF06858 NOG1 [H]

EC number: NA

Molecular weight: Translated: 40168; Mature: 40037

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNPFRKMPEVPDPEELIDVAFRRAERAAEGTRKSFYGTRTPPEVRARSIEIARVNTAC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QLVQDRLWEIVRKTPNLDELHPFYRELADALAGIDRLKSSLADVHTVAKIARLIREEYTR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIKRARDPRTAAELRRQAFGRLASTIRRKVGDALRFLRKVQPKLVDLPAIDTEMFTVTLA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCEEEEEEC
GFPNVGKTTLMTVLTGSRPEIAPYPFTTKGIQVGYMERPYPVQMLDTPGLLERPEEERNP
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCEEECCCCHHCCCHHHCCH
VERQAIAALKHVTDAVLFLIDPTGTCGYPVEEQLELLDRVRKEFDVPVYVVLTKADLRDL
HHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHH
WEEPDLEGEPVYKVSATERTGLKELRELLNDLARGHYSGRDRGHDEGRDEER
HCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGNPFRKMPEVPDPEELIDVAFRRAERAAEGTRKSFYGTRTPPEVRARSIEIARVNTAC
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QLVQDRLWEIVRKTPNLDELHPFYRELADALAGIDRLKSSLADVHTVAKIARLIREEYTR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIKRARDPRTAAELRRQAFGRLASTIRRKVGDALRFLRKVQPKLVDLPAIDTEMFTVTLA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCEEEEEEC
GFPNVGKTTLMTVLTGSRPEIAPYPFTTKGIQVGYMERPYPVQMLDTPGLLERPEEERNP
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCEEECCCCHHCCCHHHCCH
VERQAIAALKHVTDAVLFLIDPTGTCGYPVEEQLELLDRVRKEFDVPVYVVLTKADLRDL
HHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHH
WEEPDLEGEPVYKVSATERTGLKELRELLNDLARGHYSGRDRGHDEGRDEER
HCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]