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The map label for this gene is cofG [H]

Identifier: 161485663

GI number: 161485663

Start: 847218

End: 848264

Strand: Reverse

Name: cofG [H]

Synonym: MK0898

Alternate gene names: 161485663

Gene position: 848264-847218 (Counterclockwise)

Preceding gene: 20094337

Following gene: 20094333

Centisome position: 50.05

GC content: 60.94

Gene sequence:

>1047_bases
ATGGACCGGGCGAACCGGTTGACCAGGGAGAAGTTCGGCACGGTGGTTTCCTACTCGAAGAACGTGTTCGTACCGCTCAC
CCGGTTATGTCGGAATCGGTGCGCTTACTGCACCTTCCGTCGGGAACCGGAGGAGGTGAGGTCGCCGTATCTCTCCCCGG
AAGAGGTCTTCGAGATCGTGGAGAAGGGTAAGGAAGCCGGTTGCAAGGAGGTGCTCTTCACCTTCGGAGAGCGCCCTGAG
GAGCGGTACGACGAGGCCCTCGAATGGCTGGAGGAGCACGGGTATTCGAGCACCGTCGAGTACCTCGTCGACCTCTGCCG
TAGATGCGTGGAGGAGTACGACATGCTGCCGCACAGCAACCCCGGCGTGATCACGAAGCGGGAGATGCGGAAGCTGCGTC
GTTGGAACGCCAGCATGGGCCTCATGATGGAGATACTATCGGAGCGGTTGTGTGAGGAAAGTGGTCCCCACGAACACTCT
CCCGGGAAGCGCCCCGAGGAGCGTCTGAAGGTGCTGAAATACGCCGGGGAGTTGAAAGTACCTTTCACCACCGGGATACT
GATCGGCATCGGCGAGACGTGGGAGGAACGCGTGAAGACCTTGGAAGAGATCCAGAGAATGCACGAGCGGTACGGTCACG
TTCAGGAGGTGATCGTTCAGAACTTCAGGACGAAACCGGGAATCCCGATGGAGGACCACCCCGAACCCACACCCGCCGAC
TTACTACGGACGGTCGCGACGGCGCGGCTGATACTGCCGGACGTTCCGGTCCAAGTACCCCCGAACCTGAACCGTGAGAC
GGGTCAGCTCGCGCTGTTGGCGGGCGCGAACGACTGGGGCGGAGTTTCCCCCGTGACGAAGGATTACGTGAACCCCGAAG
CCCCCTGGCCCGAGATCGAGGAGCTGAAAAGACTGACCGAAGACGTCGGTTTCAGGTTAAGGGAGCGGCTCCCTATTTAC
CCCGAGTACGTCCGTCGGGGCTGGTACCATGCCAACATCTCAGAAGTCGTCGAGCGGCTCTCGGACGATGAGGGATTCGC
CCGATGA

Upstream 100 bases:

>100_bases
CCCCTTGATTACGTCTGTCCCGACGCGCTCAGGATTAAACCCCGCGGGTGACCGAAGCGACCGGGGCGGACGTTTGAAGG
GCAACGATCTCCTCGAGCTC

Downstream 100 bases:

>100_bases
AGTCAGCGTCGACGAGCTGGTGAACATGGCCGTGGCCGGTGGCATCGACGAAGGAACGGCGTTGGACGCCTTACAGGGTA
AGCTGGACCCGTACAAGGTA

Product: FO synthase subunit 1

Products: Biotin [C]

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 1 [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MDRANRLTREKFGTVVSYSKNVFVPLTRLCRNRCAYCTFRREPEEVRSPYLSPEEVFEIVEKGKEAGCKEVLFTFGERPE
ERYDEALEWLEEHGYSSTVEYLVDLCRRCVEEYDMLPHSNPGVITKREMRKLRRWNASMGLMMEILSERLCEESGPHEHS
PGKRPEERLKVLKYAGELKVPFTTGILIGIGETWEERVKTLEEIQRMHERYGHVQEVIVQNFRTKPGIPMEDHPEPTPAD
LLRTVATARLILPDVPVQVPPNLNRETGQLALLAGANDWGGVSPVTKDYVNPEAPWPEIEELKRLTEDVGFRLRERLPIY
PEYVRRGWYHANISEVVERLSDDEGFAR

Sequences:

>Translated_348_residues
MDRANRLTREKFGTVVSYSKNVFVPLTRLCRNRCAYCTFRREPEEVRSPYLSPEEVFEIVEKGKEAGCKEVLFTFGERPE
ERYDEALEWLEEHGYSSTVEYLVDLCRRCVEEYDMLPHSNPGVITKREMRKLRRWNASMGLMMEILSERLCEESGPHEHS
PGKRPEERLKVLKYAGELKVPFTTGILIGIGETWEERVKTLEEIQRMHERYGHVQEVIVQNFRTKPGIPMEDHPEPTPAD
LLRTVATARLILPDVPVQVPPNLNRETGQLALLAGANDWGGVSPVTKDYVNPEAPWPEIEELKRLTEDVGFRLRERLPIY
PEYVRRGWYHANISEVVERLSDDEGFAR
>Mature_348_residues
MDRANRLTREKFGTVVSYSKNVFVPLTRLCRNRCAYCTFRREPEEVRSPYLSPEEVFEIVEKGKEAGCKEVLFTFGERPE
ERYDEALEWLEEHGYSSTVEYLVDLCRRCVEEYDMLPHSNPGVITKREMRKLRRWNASMGLMMEILSERLCEESGPHEHS
PGKRPEERLKVLKYAGELKVPFTTGILIGIGETWEERVKTLEEIQRMHERYGHVQEVIVQNFRTKPGIPMEDHPEPTPAD
LLRTVATARLILPDVPVQVPPNLNRETGQLALLAGANDWGGVSPVTKDYVNPEAPWPEIEELKRLTEDVGFRLRERLPIY
PEYVRRGWYHANISEVVERLSDDEGFAR

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR019939
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: 2.8.1.6 [C]

Molecular weight: Translated: 40443; Mature: 40443

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRANRLTREKFGTVVSYSKNVFVPLTRLCRNRCAYCTFRREPEEVRSPYLSPEEVFEIV
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHH
EKGKEAGCKEVLFTFGERPEERYDEALEWLEEHGYSSTVEYLVDLCRRCVEEYDMLPHSN
HHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PGVITKREMRKLRRWNASMGLMMEILSERLCEESGPHEHSPGKRPEERLKVLKYAGELKV
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
PFTTGILIGIGETWEERVKTLEEIQRMHERYGHVQEVIVQNFRTKPGIPMEDHPEPTPAD
CCHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
LLRTVATARLILPDVPVQVPPNLNRETGQLALLAGANDWGGVSPVTKDYVNPEAPWPEIE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCHHH
ELKRLTEDVGFRLRERLPIYPEYVRRGWYHANISEVVERLSDDEGFAR
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDRANRLTREKFGTVVSYSKNVFVPLTRLCRNRCAYCTFRREPEEVRSPYLSPEEVFEIV
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHH
EKGKEAGCKEVLFTFGERPEERYDEALEWLEEHGYSSTVEYLVDLCRRCVEEYDMLPHSN
HHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PGVITKREMRKLRRWNASMGLMMEILSERLCEESGPHEHSPGKRPEERLKVLKYAGELKV
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
PFTTGILIGIGETWEERVKTLEEIQRMHERYGHVQEVIVQNFRTKPGIPMEDHPEPTPAD
CCHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
LLRTVATARLILPDVPVQVPPNLNRETGQLALLAGANDWGGVSPVTKDYVNPEAPWPEIE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCHHH
ELKRLTEDVGFRLRERLPIYPEYVRRGWYHANISEVVERLSDDEGFAR
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.002 {dethiobiotin}} [C]

Substrates: Dethiobiotin; sulfur [C]

Specific reaction: Dethiobiotin + sulfur = biotin [C]

General reaction: Sulfur atom transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]