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The map label for this gene is pnp [H]

Identifier: 19746859

GI number: 19746859

Start: 1674252

End: 1676384

Strand: Reverse

Name: pnp [H]

Synonym: spyM18_2014

Alternate gene names: 19746859

Gene position: 1676384-1674252 (Counterclockwise)

Preceding gene: 19746860

Following gene: 19746858

Centisome position: 88.46

GC content: 44.21

Gene sequence:

>2133_bases
ATGTCAAAACAAACCTTTACAACAACATTTGCAGGGAAACCCCTTGTTGTTGAAGTTGGTCAAGTCGCTAAGCAAGCCAA
TGGGGCAACCGTTGTTCGTTACGGAGAATCAACTGTCCTGACCGCAGCTGTCATGTCTAAGAAAATGGCAACGGGTGATT
TCTTCCCTCTTCAAGTTAATTACGAAGAAAAAATGTATGCCGCTGGTAAATTCCCAGGAGGTTTCATGAAGCGTGAGGGA
CGCCCTTCAACAGATGCGACCTTGACAGCTCGCTTGATTGACCGTCCGATTCGTCCCATGTTTGCCGAAGGTTTCCGTAA
CGAAGTACAAGTGATTAACACTGTTCTTTCTTATGATGAAAATGCTAGCGCTCCAATGGCAGCGATGTTTGGCTCATCTC
TGGCCTTGTCCATCTCAGATATTCCATTTAATGGACCGATTGCAGGTGTTCAAGTTGGCTATATTGATGGCGAGTTCATC
ATCAACCCAGATAAAGAACAAATGGAAGCGTCACTCTTAGAATTAACCGTTGCTGGTAGCAAAGAAGCCATCAATATGGT
TGAGTCTGGTGCCAAAGAATTATCAGAAGACATCATGCTTGAGGCTCTTTTAAAAGGTCACCAAGCCATTCAAGAATTGA
TTGCCTTCCAAGAACAAATCGTAGCCGTTGTTGGTAAAGAAAAAGCTGAGGTTGAACTGTTACAGGTTGATGCAGACTTA
CAAGCTGATATTGTGGCTAAGTACAATGCTCAACTGCAAAAAGCTGTTCAAGTTGAAGAGAAAAAAGCGCGTGAAGCAGC
CACAGAAGCTGTTAAAGAAATGGTTAAGGCTGAATATGAAGAGCGTTACGCAGAAGACGAAAACCTAGCAACCATTATGC
GTGACGTGGCAGAAATCCTTGAACAAATGGAGCATGCTGAAGTACGTCGCCTCATTACAGAAGACAAGATTCGTCCTGAC
GGTCGTAAGATTGACGAAATTCGTCCGCTTGATGCTGTGGTTGACTTCTTGCCAAAGGTCCATGGATCAGGTCTTTTTAC
GCGCGGTCAAACGCAGGCCTTGTCAGTTTTGACTTTGGCGCCAATGGGAGAAACTCAAATCATTGATGGTCTGGCTCCAG
AGTACAAAAAAAGCTTTTTACACCACTACAATTTCCCACAATATTCAGTTGGTGAAACTGGTCGTTACGGAGCTGCTGGT
CGTCGTGAAATTGGACACGGTGCCTTAGGTGAACGCGCTCTTGAACAAGTCTTGCCAAGCTTAGAAGAATTTCCTTATGC
TATTCGTTTAGTTGCCGAAGTTCTAGAATCTAACGGTTCGTCTTCTCAGGCTTCTATCTGTGCTGGTACCCTTGCTCTGA
TGGCTGGTGGTGTACCAATCAAGGCTCCTGTGGCAGGGATTGCCATGGGTCTCATTTCAGACGGGACGAATTATACTGTC
TTGACTGACATTCAGGGTCTTGAAGACCACTTTGGAGATATGGACTTTAAAGTGGCAGGAACTCGTGAAGGGATTACAGC
CCTTCAAATGGATATTAAGATTGCAGGGATTACGCCTCAAATCCTAGAAGAAGCTCTTGCACAAGCTAAAAAAGCTCGCT
TTGAAATCCTTGATGTGATTGAAGCAACTATCGCTGAACCACGTCCTGAGTTAGCCCCAACTGCACCGAAAATTGACACC
ATCAAGATTGACGTAGACAAAATCAAAGTGGTTATCGGTAAAGGTGGCGAAACTATTGACAAGATTATTGCCGAAACGGG
CGTCAAAATTGATATTGACGACGAAGGAAATGTCTCTATCTACTCAAGTGACCAAGCTGCCATTGACCGCACTAAAGAAA
TTATTGCAGGTCTTGTTCGTGAAGCCAAGGTAGGCGAAGTTTACCATGCCAAGGTTATCCGTATCGAGAAATTTGGTGCC
TTTGTCAACCTCTTTGACAAGACAGATGCACTTGTTCACATTTCTGAAATTGCTTGGACTAGAACGACCAACGTGTCAGA
TGTCCTTGAAGTGGGTGAAGACGTTGATGTTAAGGTCATTAAGATTGATGAGAAAGGTCGTGTGGATGCCTCAATGAAAG
CTTTGATTCCACGCCCACCAAAACCAGAGAAAAAAGAAGAAAAACATGACTAA

Upstream 100 bases:

>100_bases
GCGTGGCAGGTTGCTTTTTGTAGCAAAACAACGTATAACAACTGAATCTAAGCAGAGAACAGGTACCTCTTTACCCCTCT
GAAAATTAAAAGGAGAATAT

Downstream 100 bases:

>100_bases
ATCAAATGAATTAGATATTCGCTTAAGAGCTTTTATCAATGCGCCTGATAACTTTTTGGATAGCCTTGCGCTTGTCAACG
CTTTCCATAATTTTCCTGTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREG
RPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQEQIVAVVGKEKAEVELLQVDADL
QADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVKAEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPD
GRKIDEIRPLDAVVDFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKSFLHHYNFPQYSVGETGRYGAAG
RREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTV
LTDIQGLEDHFGDMDFKVAGTREGITALQMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDT
IKIDVDKIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAIDRTKEIIAGLVREAKVGEVYHAKVIRIEKFGA
FVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKIDEKGRVDASMKALIPRPPKPEKKEEKHD

Sequences:

>Translated_710_residues
MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREG
RPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQEQIVAVVGKEKAEVELLQVDADL
QADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVKAEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPD
GRKIDEIRPLDAVVDFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKSFLHHYNFPQYSVGETGRYGAAG
RREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTV
LTDIQGLEDHFGDMDFKVAGTREGITALQMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDT
IKIDVDKIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAIDRTKEIIAGLVREAKVGEVYHAKVIRIEKFGA
FVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKIDEKGRVDASMKALIPRPPKPEKKEEKHD
>Mature_709_residues
SKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGR
PSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFII
NPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQEQIVAVVGKEKAEVELLQVDADLQ
ADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVKAEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDG
RKIDEIRPLDAVVDFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKSFLHHYNFPQYSVGETGRYGAAGR
REIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTVL
TDIQGLEDHFGDMDFKVAGTREGITALQMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTI
KIDVDKIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAIDRTKEIIAGLVREAKVGEVYHAKVIRIEKFGAF
VNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKIDEKGRVDASMKALIPRPPKPEKKEEKHD

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=712, Percent_Identity=35.6741573033708, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI145693187, Length=689, Percent_Identity=48.766328011611, Blast_Score=619, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI115534063, Length=713, Percent_Identity=34.0813464235624, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI281362905, Length=697, Percent_Identity=37.0157819225251, Blast_Score=442, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651641, Length=697, Percent_Identity=37.0157819225251, Blast_Score=442, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651643, Length=697, Percent_Identity=37.0157819225251, Blast_Score=442, Evalue=1e-124,
Organism=Drosophila melanogaster, GI161079377, Length=661, Percent_Identity=36.4599092284418, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77328; Mature: 77197

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAVMSKKMATGDFFPLQVN
CCCCEEEEEECCCCEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEC
YEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDE
CHHHHEECCCCCCCHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
NASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFIINPDKEQMEASLLELTVAGS
CCCCCHHHHCCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHEEECCC
KEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQEQIVAVVGKEKAEVELLQVDADL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
QADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVKAEYEERYAEDENLATIMRDVAEIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVVDFLPKVHGSGLFTRGQTQALSVLTLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCHHHEEEEEEC
PMGETQIIDGLAPEYKKSFLHHYNFPQYSVGETGRYGAAGRREIGHGALGERALEQVLPS
CCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTV
HHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
LTDIQGLEDHFGDMDFKVAGTREGITALQMDIKIAGITPQILEEALAQAKKARFEILDVI
EEECCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EATIAEPRPELAPTAPKIDTIKIDVDKIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSI
HHHHCCCCCCCCCCCCCCEEEEEEHEEEEEEEECCCHHHHHHHHHCCCEEEECCCCCEEE
YSSDQAAIDRTKEIIAGLVREAKVGEVYHAKVIRIEKFGAFVNLFDKTDALVHISEIAWT
EECCHHHHHHHHHHHHHHHHHHHCCCEEHHEEEEEEHHHHHHHHHCCCCCEEEEHHHHEE
RTTNVSDVLEVGEDVDVKVIKIDEKGRVDASMKALIPRPPKPEKKEEKHD
CCCCHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAVMSKKMATGDFFPLQVN
CCCEEEEEECCCCEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEC
YEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDE
CHHHHEECCCCCCCHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
NASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFIINPDKEQMEASLLELTVAGS
CCCCCHHHHCCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHEEECCC
KEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQEQIVAVVGKEKAEVELLQVDADL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
QADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVKAEYEERYAEDENLATIMRDVAEIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVVDFLPKVHGSGLFTRGQTQALSVLTLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCHHHEEEEEEC
PMGETQIIDGLAPEYKKSFLHHYNFPQYSVGETGRYGAAGRREIGHGALGERALEQVLPS
CCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTV
HHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
LTDIQGLEDHFGDMDFKVAGTREGITALQMDIKIAGITPQILEEALAQAKKARFEILDVI
EEECCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EATIAEPRPELAPTAPKIDTIKIDVDKIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSI
HHHHCCCCCCCCCCCCCCEEEEEEHEEEEEEEECCCHHHHHHHHHCCCEEEECCCCCEEE
YSSDQAAIDRTKEIIAGLVREAKVGEVYHAKVIRIEKFGAFVNLFDKTDALVHISEIAWT
EECCHHHHHHHHHHHHHHHHHHHCCCEEHHEEEEEEHHHHHHHHHCCCCCEEEEHHHHEE
RTTNVSDVLEVGEDVDVKVIKIDEKGRVDASMKALIPRPPKPEKKEEKHD
CCCCHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA