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The map label for this gene is thiD [H]

Identifier: 19746821

GI number: 19746821

Start: 1631248

End: 1632012

Strand: Reverse

Name: thiD [H]

Synonym: spyM18_1965

Alternate gene names: 19746821

Gene position: 1632012-1631248 (Counterclockwise)

Preceding gene: 19746822

Following gene: 19746820

Centisome position: 86.12

GC content: 35.69

Gene sequence:

>765_bases
ATGAAAACTGATTATATCGTGACTATATCTGGGAACGATATTTTAAGTGGAGGTGGCCTCTATGCTGATTTAGCGACTTA
CATAAGGTATGACCTACAGGCCTTTGTAGCGGTAACTTGTTTAACTACTAGATCTGAAGAGGGCTTTAGTCTATTTCCGG
TAGCTAAAGAAATTTTCCGTGATCAGTTGAACAGCTTTACGAACGCTCCAATTTCTGCTATTAAAATTGGTTTGTTACCT
AATGCGGAAATGTGTGAAATAGTTTTGGATTTTATAAAGGGGCATGTGGGAATCCCAGTGGTTTTAGATCCTGTTCTAGC
ATGTAAAGAAATAGATGATGTTAAAATAGTTCCTCTAAGACAGGAGATTTTACAATTATTGCCTTATGTAACTGTTGTTA
CTCCAAATTTGGTAGAGGCTCAGCTATTATCTCAAAAAGAGATTGTTTCTTTAAAAGATATGCAAGAAGCTGCTAAATAT
TTTTATCAACTTGGGGCAAAACAGGTGGTTATCAAGGGTGGTAATCGATTTAGTCAGAAAAAGGCTATTGATCTTTTTTA
TGACGGTAAAGAGATTGTTACCTTGGAATGTCCTGTACTTGAGAAAAATAATATTGGCGCAGGCTGTACTTTTGCATCTA
GTATCGCTAGTCAGTTAGTAAAGAAAAAAACGCCACTAGAAGCTGTGAAAAACTCAAAAGAGTTAGTCTACCAAGCTATT
TTACAATCTGATAGATACGGAGTAAAACAAAGTTATGCAAAATAG

Upstream 100 bases:

>100_bases
ATGCCAGTTGAACAAGTTAAAGTGATTTTGAGCTCCAAAAATCGTCAGCTAGCTGGACCAACGATATCTGGAAATGGGCT
TTACTTGAAGGAGATTTGTT

Downstream 100 bases:

>100_bases
TAAGATACGACAAATGAGTTTAACAGGGATATTGACAGCATTGGTTGTTGTTTTAGGAAGATTTGTCATGCTACCTACTC
CAACAGGTTTTTTAACGCTG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTRSEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLP
NAEMCEIVLDFIKGHVGIPVVLDPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQLVKKKTPLEAVKNSKELVYQAI
LQSDRYGVKQSYAK

Sequences:

>Translated_254_residues
MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTRSEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLP
NAEMCEIVLDFIKGHVGIPVVLDPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQLVKKKTPLEAVKNSKELVYQAI
LQSDRYGVKQSYAK
>Mature_254_residues
MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTRSEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLP
NAEMCEIVLDFIKGHVGIPVVLDPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQLVKKKTPLEAVKNSKELVYQAI
LQSDRYGVKQSYAK

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=251, Percent_Identity=27.8884462151394, Blast_Score=91, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6325378, Length=254, Percent_Identity=25.9842519685039, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 28034; Mature: 28034

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTRSEEGFSLFPVAKEIFR
CCCCEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHH
DQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHVGIPVVLDPVLACKEIDDVKIVPLR
HHHHHHCCCCHHHEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEHHH
QEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKYFYQLGAKQVVIKGGNRFSQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
KAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQLVKKKTPLEAVKNSKELVYQAI
HCEEEEECCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHH
LQSDRYGVKQSYAK
HHCCCCCCHHHCCC
>Mature Secondary Structure
MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTRSEEGFSLFPVAKEIFR
CCCCEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHH
DQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHVGIPVVLDPVLACKEIDDVKIVPLR
HHHHHHCCCCHHHEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEHHH
QEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKYFYQLGAKQVVIKGGNRFSQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
KAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQLVKKKTPLEAVKNSKELVYQAI
HCEEEEECCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHH
LQSDRYGVKQSYAK
HHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA