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The map label for this gene is isdE [H]

Identifier: 19746741

GI number: 19746741

Start: 1541894

End: 1543636

Strand: Reverse

Name: isdE [H]

Synonym: spyM18_1867

Alternate gene names: 19746741

Gene position: 1543636-1541894 (Counterclockwise)

Preceding gene: 19746742

Following gene: 19746740

Centisome position: 81.46

GC content: 38.21

Gene sequence:

>1743_bases
ATGACAAAGGTAGTAATAAAGCAATTGTTGCAGGTAATAGTAGTGTTCATGATAAGTTTATCAACAATGACAAATCTTGT
GTATGCAGATAAAGGTCAAATTTATGGATGTATTATTCAAAGAAATTATCGTCACCCGATATCTGGGCAGATAGAAGATA
GTGGAGGGGAGCATTCCTTTGATATTGGTCAAGGTATGGTTGAAGGGACTGTCTATAGTGATGCCATGCTAGAAGTATCT
GATGCTGGAAAGATCGTTCTTACTTTTCGTATGAGCTTAGCTGATTATTCGGGTAATTATCAGTTCTGGATACAACCTGG
AGGAACTGGAAGCTTTCAGGCAGTTGATTACAATATCACCCAAAAAGGTACTGATACCAATGGGACAACACTAGATATTG
CTATTAGTTTGCCTACTGTAAATAGTATCATTCGAGGAAGCATGTTTGTTGAACCTATGGGCAGAGAAGTGGTCTTTTAC
CTGTCAGCTTCAGAGTTAATACAAAAGTATTCAGGTAATATGTTTGCCCAGCTTGTTACTGAAACAGACAATTCTCAAAA
TCAAGAAGTAAAAGACAGTCAAAAGCCTGTTGATACAAAACTAGGAGAAAGTCAAGATGAGTCTCACACAGGTGCTATGA
TAACTCAGAATAAGCCTAAAGCCAACTCATCAAATAACAAGAGTCTATCAGATAAAAAAATTCTTCCTTCTAAGATGGGC
TTAACGACTTCTTTAGAGTTAAAAAAAGAAGATAAGTTTCGGTCTAAAAAAGACTTATCAATCATGATCTACTACTTTCC
AACTTTTTTTCTGATGTTAGGAGGGTTTGCTGTATGGGTTTGGAAAAAGAGGAAAAAAATGATAAAACGATGTAAAGGAA
TTGGTCTAGTCTTAATGGCCTTCTTTTTGGTAGCTTGTGTAAATCAGCACCCTAAAACGGCTAAAGAGACTGAACAGCAG
AGAATTGTAGCCACTTCGGTTGCTGTGGTTGATATCTGTGACCGTTTAAATTTAGACCTCGTTGGGGTTTGTGATAGTAA
ATTATATACCCTTCCTAAACGCTATGATGCTGTTAAGCGTGTGGGTTTACCCATGAATCCTGATATAGAGTTGATTGCTT
CTTTGAAACCAACTTGGATTTTGAGTCCCAATTCTTTACAAGAAGATTTGGAACCCAAGTATCAAAAATTGGATACTGAG
TATGGTTTTTTGAACTTACGAAGTGTTGAGGGCATGTACCAGTCTATTGATGATTTAGGGAACCTTTTCCAACGTCAACA
AGAAGCAAAAGAATTGCGCCAGCAATACCAGGACTATTATCGTGCTTTCCAAGCTAAACGTAAGGGGAAGAAAAAGCCTA
AAGTGCTTATTCTTATGGGCTTGCCAGGTAGTTATTTGGTGGCGACGAACCAATCTTATGTAGGGAATCTTTTGGACTTG
GCAGGTGGTGAGAATGTTTATCAGTCAGATGAGAAAGAATTTCTATCAGTTAATCCTGAAGACATGCTAGCTAAGGAGCC
TGACTTGATTTTACGAACAGCTCACGCCATTCCAGACAAGGTAAAAGTGATGTTTGACAAAGAATTTGCTGAAAATGATA
TTTGGAAACATTTTACGGCAGTCAAGGAAGGGAAAGTCTATGATTTGGACAATACCCTGTTTGGCATGAGTGCTAAATTG
AACTACCCAGAAGCCTTGGACACCTTAACACAGCTTTTTGACCACGTGGGAGATCATCCGTAA

Upstream 100 bases:

>100_bases
CGCAAGATAGGTGTGTTTTTCACATTTGGAGATGTTATAAAATGGGAATCATTTAAAAAATGAAATAAGATGTTTAAGAA
AAGATAGACAAGAGGAGCAT

Downstream 100 bases:

>100_bases
CATGACAGCATCAGATTTACAAAGGGATCAATCCCTATTGACTAAAAAACGTTTCAGAAGTGGTCTTTATTTAGTTCTTT
TAATTCTCAGCCTCCTATTT

Product: ABC transporter

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: Iron-regulated surface determinant protein E; Staphylococcal iron-regulated protein F [H]

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MTKVVIKQLLQVIVVFMISLSTMTNLVYADKGQIYGCIIQRNYRHPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVS
DAGKIVLTFRMSLADYSGNYQFWIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
LSASELIQKYSGNMFAQLVTETDNSQNQEVKDSQKPVDTKLGESQDESHTGAMITQNKPKANSSNNKSLSDKKILPSKMG
LTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGFAVWVWKKRKKMIKRCKGIGLVLMAFFLVACVNQHPKTAKETEQQ
RIVATSVAVVDICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQEDLEPKYQKLDTE
YGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAKRKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDL
AGGENVYQSDEKEFLSVNPEDMLAKEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKL
NYPEALDTLTQLFDHVGDHP

Sequences:

>Translated_580_residues
MTKVVIKQLLQVIVVFMISLSTMTNLVYADKGQIYGCIIQRNYRHPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVS
DAGKIVLTFRMSLADYSGNYQFWIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
LSASELIQKYSGNMFAQLVTETDNSQNQEVKDSQKPVDTKLGESQDESHTGAMITQNKPKANSSNNKSLSDKKILPSKMG
LTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGFAVWVWKKRKKMIKRCKGIGLVLMAFFLVACVNQHPKTAKETEQQ
RIVATSVAVVDICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQEDLEPKYQKLDTE
YGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAKRKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDL
AGGENVYQSDEKEFLSVNPEDMLAKEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKL
NYPEALDTLTQLFDHVGDHP
>Mature_579_residues
TKVVIKQLLQVIVVFMISLSTMTNLVYADKGQIYGCIIQRNYRHPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSD
AGKIVLTFRMSLADYSGNYQFWIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFYL
SASELIQKYSGNMFAQLVTETDNSQNQEVKDSQKPVDTKLGESQDESHTGAMITQNKPKANSSNNKSLSDKKILPSKMGL
TTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGFAVWVWKKRKKMIKRCKGIGLVLMAFFLVACVNQHPKTAKETEQQR
IVATSVAVVDICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQEDLEPKYQKLDTEY
GFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAKRKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLA
GGENVYQSDEKEFLSVNPEDMLAKEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLN
YPEALDTLTQLFDHVGDHP

Specific function: Involved in heme (porphyrin) scavenging. Binds Fe(2+) and Fe(3+) heme but the largest fraction is Fe(2+) heme. Functions as a high-affinity heme binding protein and probably has a role in relaying heme-iron from cell wall-anchored isd proteins receptors t

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019957
- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 65346; Mature: 65215

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVVIKQLLQVIVVFMISLSTMTNLVYADKGQIYGCIIQRNYRHPISGQIEDSGGEHSF
CCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCCCCCCEECCCCCCCCC
DIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQFWIQPGGTGSFQAVDYNIT
HHCCCCCCCEEECCCEEEECCCCEEEEEEEEEHHCCCCCEEEEEEECCCCCEEEEEEEEE
QKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFYLSASELIQKYSGNMFAQLVT
CCCCCCCCCEEEEEEECCCHHHHHHCCEEECCCCCEEEEEECHHHHHHHHCCCEEEEEEE
ETDNSQNQEVKDSQKPVDTKLGESQDESHTGAMITQNKPKANSSNNKSLSDKKILPSKMG
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHCC
LTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGFAVWVWKKRKKMIKRCKGIGLVLMA
CCEEEEECHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FFLVACVNQHPKTAKETEQQRIVATSVAVVDICDRLNLDLVGVCDSKLYTLPKRYDAVKR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCHHHHHHHHH
VGLPMNPDIELIASLKPTWILSPNSLQEDLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLG
CCCCCCCCHHEEECCCCEEEECCCHHHHHCCHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
NLFQRQQEAKELRQQYQDYYRAFQAKRKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECHHHHHHHHHH
AGGENVYQSDEKEFLSVNPEDMLAKEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTA
CCCCCCCCCCCHHHCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHH
VKEGKVYDLDNTLFGMSAKLNYPEALDTLTQLFDHVGDHP
HCCCCEEECCCCEECCEECCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKVVIKQLLQVIVVFMISLSTMTNLVYADKGQIYGCIIQRNYRHPISGQIEDSGGEHSF
CHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCCCCCCEECCCCCCCCC
DIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQFWIQPGGTGSFQAVDYNIT
HHCCCCCCCEEECCCEEEECCCCEEEEEEEEEHHCCCCCEEEEEEECCCCCEEEEEEEEE
QKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFYLSASELIQKYSGNMFAQLVT
CCCCCCCCCEEEEEEECCCHHHHHHCCEEECCCCCEEEEEECHHHHHHHHCCCEEEEEEE
ETDNSQNQEVKDSQKPVDTKLGESQDESHTGAMITQNKPKANSSNNKSLSDKKILPSKMG
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHCC
LTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGFAVWVWKKRKKMIKRCKGIGLVLMA
CCEEEEECHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FFLVACVNQHPKTAKETEQQRIVATSVAVVDICDRLNLDLVGVCDSKLYTLPKRYDAVKR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCHHHHHHHHH
VGLPMNPDIELIASLKPTWILSPNSLQEDLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLG
CCCCCCCCHHEEECCCCEEEECCCHHHHHCCHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
NLFQRQQEAKELRQQYQDYYRAFQAKRKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECHHHHHHHHHH
AGGENVYQSDEKEFLSVNPEDMLAKEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTA
CCCCCCCCCCCHHHCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHH
VKEGKVYDLDNTLFGMSAKLNYPEALDTLTQLFDHVGDHP
HCCCCEEECCCCEECCEECCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA