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The map label for this gene is trmB [H]

Identifier: 19746628

GI number: 19746628

Start: 1443432

End: 1444067

Strand: Reverse

Name: trmB [H]

Synonym: spyM18_1734

Alternate gene names: 19746628

Gene position: 1444067-1443432 (Counterclockwise)

Preceding gene: 19746629

Following gene: 19746627

Centisome position: 76.2

GC content: 39.94

Gene sequence:

>636_bases
ATGCGAGTTAGAAAACGAAAAGGTGCTGAGAAGCACTTAGCTAATAACCCACACTATGTGATATTAAATCCAGAAGATGC
CAAGGGCCGTTGGCATGACGTTTTTGGAAATGACCGTCCTATTCATATCGAAGTTGGTTCAGGAAAGGGCGGATTTATCA
CAGGGATGGCCTTGAAAAATCCTGATATTAACTACATTGGCATTGATATTCAATTATCTGTCCTAAGCTACGCCTTGGAT
AAGGTATTAGCTTCAGAAGTACCCAATGTCAAACTTCTGCGAGTGGATGGATCAAGTTTAACCAACTATTTTGAAGATGG
TGAGGTGGATATGATGTACCTCAACTTTTCAGATCCTTGGCCAAAAACCAAGCATGAAAAACGTCGTTTGACCTACAAGG
ATTTCTTAGACACTTACAAGCGTATCTTGCCAGAACACGGTGAAATTCATTTTAAAACCGATAATCGGGGCTTGTTTGAG
TACAGTTTAGCTAGTTTTTCTCAATACGGCATGACCTTGAGACAGATCTGGCTTGACTTACACGCTAGTAACTATGAAGG
AAATGTCATGACAGAATATGAAGAGAAGTTCTCTAATAAAGGTCAAGTCATTTACCGAGTTGAGGCAAATTTCTAG

Upstream 100 bases:

>100_bases
TGACACAAATTCTCAAGTGTTTTGACAAGCGTGACATGGAGCATGTGAATCAGGAGATTTATGCCCTCAGAAAATTTAGA
GAAATATTTAGAAAGAAATA

Downstream 100 bases:

>100_bases
TAGTATTTTGTTTCAAAATATGATAACATAAAGGAGTTGATACAGTATTAACTTCTTTAGTAATGGAGAGGTCGCATAGT
GGCTTAGTGCGCACGCTTGG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MRVRKRKGAEKHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIEVGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALD
KVLASEVPNVKLLRVDGSSLTNYFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF

Sequences:

>Translated_211_residues
MRVRKRKGAEKHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIEVGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALD
KVLASEVPNVKLLRVDGSSLTNYFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF
>Mature_211_residues
MRVRKRKGAEKHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIEVGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALD
KVLASEVPNVKLLRVDGSSLTNYFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=135, Percent_Identity=28.1481481481481, Blast_Score=67, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17536689, Length=140, Percent_Identity=30, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 24454; Mature: 24454

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRKRKGAEKHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIEVGSGKGGFITGMALKN
CCCCCCCCCHHHHCCCCCEEEECCCCCCCCEEECCCCCCEEEEEECCCCCCEEEEEEECC
PDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTNYFEDGEVDMMYLNFSDPW
CCCCEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCCEEEEEEECCCCC
PKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFEYSLASFSQYGMTLRQIWLDL
CCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHH
HASNYEGNVMTEYEEKFSNKGQVIYRVEANF
CCCCCCCCEEEHHHHHHCCCCCEEEEEECCC
>Mature Secondary Structure
MRVRKRKGAEKHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIEVGSGKGGFITGMALKN
CCCCCCCCCHHHHCCCCCEEEECCCCCCCCEEECCCCCCEEEEEECCCCCCEEEEEEECC
PDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTNYFEDGEVDMMYLNFSDPW
CCCCEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCCEEEEEEECCCCC
PKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFEYSLASFSQYGMTLRQIWLDL
CCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHH
HASNYEGNVMTEYEEKFSNKGQVIYRVEANF
CCCCCCCCEEEHHHHHHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]