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The map label for this gene is glyS [H]

Identifier: 19746599

GI number: 19746599

Start: 1414291

End: 1416330

Strand: Reverse

Name: glyS [H]

Synonym: spyM18_1700

Alternate gene names: 19746599

Gene position: 1416330-1414291 (Counterclockwise)

Preceding gene: 19746600

Following gene: 19746598

Centisome position: 74.74

GC content: 43.73

Gene sequence:

>2040_bases
ATGAGTAAAAATCTACTAATCGAACTTGGTCTGGAAGAACTTCCAGCCTACGTGGTCACACCAAGTGAAAAACAACTGGG
AGAGCGCCTGGCGACTTTCTTAACAGAAAATCGTCTCTCTTTTGAAGATATACAAACCTTTTCAACTCCACGCCGTTTGG
CAGTGCGTGTGTCTGGGTTAGCTGATCAGCAAACGGATTTGACTGAGGACTTTAAAGGGCCTGCTAAGAAAATTGCCCTT
GATGCTGATGGGAATTTCTCAAAAGCTGCTCAAGGATTTGTTCGTGGCAAAGGCTTGACCACAGATGCTATCGAATTCCG
CGAAGTCAAAGGAGAAGAATACGTTTATGTGACCAAACACGAAGCTGGAAAACCTGCTAAGGAAGTGCTTCTAGGCGTTA
CTGAAGTGTTGTCAGCAATGACCTTCCCAGTCAGCATGCACTGGGCTAACAATAGTTTTGAATACATTCGTCCTGTTCAC
ACCTTGACAGTTCTGTTGAATGATGAGGCCTTGGAACTTGACTTCTTGGATATTCATTCTGGACGCGTCAGCCGTGGTCA
CCGTTTTTTAGGTACTGAAACCACCATCACAAGTGCTGATTCTTATGAGGCTGATTTGCGCAGCCAGTTTGTTATCGCTG
ATGCTAAAGAGCGTCAAGAGATGATTGTGGAGCAAATCAAGACGCTTGAAGTGGAGCAGGGTGTGCAAGTTGACATTGAC
GAAGACTTGCTTAATGAAGTTTTGAACTTGGTCGAGTTTCCAACAGCTTTCATGGGAAGTTTTGAGGCTAAATACCTTGA
TGTCCCAGAAGAAGTATTGGTAACATCAATGAAAAACCACCAACGTTATTTTGTAGTACGTGATCAGGCTGGTCACTTGA
TGCCAAACTTTGTATCAGTTCGTAATGGGAATGATCAAGCCATTGAAAATGTTATTAAAGGGAACGAAAAAGTTTTGGTA
GCTCGCTTAGAAGATGGTGAATTCTTCTGGCGTGAGGATCAGAAATTGCAAATTGCTGATTTGGTTGCCAAATTGACCAA
TGTGACCTTCCATGAAAAAATCGGCTCTCTTGCTGAACACATGGATCGTACGCGTGTCATTGCCGCATCACTTGCTAAGG
AAGCCAACCTATCTGCTGAGGAAGTCACAGCTGTTGACCGTGCTGCCCAAATTTACAAATTTGACCTTTTAACAGGTATG
GTTGGTGAATTTGATGAGCTGCAAGGGATTATGGGTGAAAAATATGCTTTATTAGCTGGTGAAGATGCTGCTGTGGCAAC
AGCAATCCGTGAACACTACTTACCTGATGCTGCAGGAGGTGCGCTACCTGAAACCAAAGTTGGCGCTGTCCTGGCCTTGG
CAGATAAATTGGATACTCTCTTGTCATTCTTTTCTGTTGGCTTGATTCCATCTGGTTCAAACGACCCTTATGCGCTTCGT
CGGGCAACGCAGGGTATCGTTCGTATTCTTGATCATTTTGGATGGCGCATCCCAATGGATAAGCTGGTAGATAGCTTATA
TGACTTGTCCTTTGATAGTTTGACCTATGCCAATAAGGCGGATGTGATGAACTTTATCCGTGCTCGTGTGGATAAGATGA
TGGGCAAAGCCGCACCAAAAGATATTCGTGAAGCAGTTTTAGAGAGTTCAACCTTTGTGGTTCCTGAAATGTTAGCGGCA
GCTGAAGCACTTGTCAAAGCCAGCCATACGGAAAATTACAAACCAGCAGTTGAATCTTTGTCACGTGCTTTTAATTTGGC
AGAAAAAGCGGACGCTTCTGTTCAGGTGGACCCTAGCCTCTTTGAAAATGAGCAAGAAAACACTTTGTTTGCAGCTATTC
AAGGCTTGACTCTAGCAGGCAGTGCTGCCCAGCAGTTAGAACAGGTCTTTGCCCTTAGCCCAGTGATTAACGATTTCTTT
GATAACACAATGGTTATGGCAGAAGATCAAGCCCTTAAGAACAACCGTTTAGCTATTTTATCAGATTTAGTAAGTAAAGC
TAAGACCATTGCTGCTTTTAACCAACTAAACACCAAGTAA

Upstream 100 bases:

>100_bases
CTACGGACTATGTCAAAAAGACCAAGACTTCCTAGGTGCAAGCACCTGCGTCGCTTTGCTATTTTGATGGTGTCCACTTA
ACGCCCTTGGTATCTTAATT

Downstream 100 bases:

>100_bases
ATAGAAAGGAAAGAGCCTTTGGGGACTTAGCTAGAAAAGTGAGTTCAGTAAAGAACAAGGCCTTTAAACATTGCTTTTCA
CAGTGAGAGTCAAGTCAGTT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDIQTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIAL
DADGNFSKAAQGFVRGKGLTTDAIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKERQEMIVEQIKTLEVEQGVQVDID
EDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTSMKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLV
ARLEDGEFFWREDQKLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIYKFDLLTGM
VGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAVLALADKLDTLLSFFSVGLIPSGSNDPYALR
RATQGIVRILDHFGWRIPMDKLVDSLYDLSFDSLTYANKADVMNFIRARVDKMMGKAAPKDIREAVLESSTFVVPEMLAA
AEALVKASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQQLEQVFALSPVINDFF
DNTMVMAEDQALKNNRLAILSDLVSKAKTIAAFNQLNTK

Sequences:

>Translated_679_residues
MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDIQTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIAL
DADGNFSKAAQGFVRGKGLTTDAIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKERQEMIVEQIKTLEVEQGVQVDID
EDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTSMKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLV
ARLEDGEFFWREDQKLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIYKFDLLTGM
VGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAVLALADKLDTLLSFFSVGLIPSGSNDPYALR
RATQGIVRILDHFGWRIPMDKLVDSLYDLSFDSLTYANKADVMNFIRARVDKMMGKAAPKDIREAVLESSTFVVPEMLAA
AEALVKASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQQLEQVFALSPVINDFF
DNTMVMAEDQALKNNRLAILSDLVSKAKTIAAFNQLNTK
>Mature_678_residues
SKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDIQTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALD
ADGNFSKAAQGFVRGKGLTTDAIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVHT
LTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKERQEMIVEQIKTLEVEQGVQVDIDE
DLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTSMKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVA
RLEDGEFFWREDQKLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIYKFDLLTGMV
GEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAVLALADKLDTLLSFFSVGLIPSGSNDPYALRR
ATQGIVRILDHFGWRIPMDKLVDSLYDLSFDSLTYANKADVMNFIRARVDKMMGKAAPKDIREAVLESSTFVVPEMLAAA
EALVKASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQQLEQVFALSPVINDFFD
NTMVMAEDQALKNNRLAILSDLVSKAKTIAAFNQLNTK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=681, Percent_Identity=35.8296622613803, Blast_Score=357, Evalue=2e-99,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75063; Mature: 74932

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDIQTFSTPRRLAVRVSGL
CCCCEEEEECHHHCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHEEEEECCC
ADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTDAIEFREVKGEEYVYVTKH
CCCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECC
EAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVHTLTVLLNDEALELDFLDIHS
CCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCCEEEEEEEECCCEEEEEEEECCC
GRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKERQEMIVEQIKTLEVEQGVQVDID
CCCCCCCEEECCCCEEECCCCCCHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCEEECC
EDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTSMKNHQRYFVVRDQAGHLMPNFVSV
HHHHHHHHHHHHCCHHHHCCCCCHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEE
RNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQKLQIADLVAKLTNVTFHEKIGSLAEH
CCCCHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
MDRTRVIAASLAKEANLSAEEVTAVDRAAQIYKFDLLTGMVGEFDELQGIMGEKYALLAG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEC
EDAAVATAIREHYLPDAAGGALPETKVGAVLALADKLDTLLSFFSVGLIPSGSNDPYALR
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RATQGIVRILDHFGWRIPMDKLVDSLYDLSFDSLTYANKADVMNFIRARVDKMMGKAAPK
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
DIREAVLESSTFVVPEMLAAAEALVKASHTENYKPAVESLSRAFNLAEKADASVQVDPSL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEECHHH
FENEQENTLFAAIQGLTLAGSAAQQLEQVFALSPVINDFFDNTMVMAEDQALKNNRLAIL
HCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCHHHH
SDLVSKAKTIAAFNQLNTK
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDIQTFSTPRRLAVRVSGL
CCCEEEEECHHHCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHEEEEECCC
ADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTDAIEFREVKGEEYVYVTKH
CCCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECC
EAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVHTLTVLLNDEALELDFLDIHS
CCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCCEEEEEEEECCCEEEEEEEECCC
GRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKERQEMIVEQIKTLEVEQGVQVDID
CCCCCCCEEECCCCEEECCCCCCHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCEEECC
EDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTSMKNHQRYFVVRDQAGHLMPNFVSV
HHHHHHHHHHHHCCHHHHCCCCCHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEE
RNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQKLQIADLVAKLTNVTFHEKIGSLAEH
CCCCHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
MDRTRVIAASLAKEANLSAEEVTAVDRAAQIYKFDLLTGMVGEFDELQGIMGEKYALLAG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEC
EDAAVATAIREHYLPDAAGGALPETKVGAVLALADKLDTLLSFFSVGLIPSGSNDPYALR
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RATQGIVRILDHFGWRIPMDKLVDSLYDLSFDSLTYANKADVMNFIRARVDKMMGKAAPK
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
DIREAVLESSTFVVPEMLAAAEALVKASHTENYKPAVESLSRAFNLAEKADASVQVDPSL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEECHHH
FENEQENTLFAAIQGLTLAGSAAQQLEQVFALSPVINDFFDNTMVMAEDQALKNNRLAIL
HCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCHHHH
SDLVSKAKTIAAFNQLNTK
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]