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The map label for this gene is rsmB [H]

Identifier: 19746552

GI number: 19746552

Start: 1361409

End: 1362731

Strand: Reverse

Name: rsmB [H]

Synonym: spyM18_1637

Alternate gene names: 19746552

Gene position: 1362731-1361409 (Counterclockwise)

Preceding gene: 19746553

Following gene: 19746551

Centisome position: 71.91

GC content: 38.78

Gene sequence:

>1323_bases
TTGGCAGATAATTGGAAAAAAAGTACGCGAGGCAAAGCCCTCCTTGTCATTGAAGCTATTTTTGATCAGGGGGCTTACAC
AAACATAGCGCTTAATCAGCAGCTAAGCAATAAAGCTTTATCTGCTAAGGACAGAGCTCTATTAACTGAGATTGTATATG
GTACCGTTTCACGTAAAATTAGCTTGGAATGGTATTTAGCCCATTATGTCAAAGACCGAGATAAATTGGACAAATGGGTT
TATTATCTCCTTATGCTCAGTCTTTATCAACTGATTTATTTAGATAAGTTACCAGCGCATGCCATTGTTAACGATGCGGT
TGGTATTGCCAAAAATCGTGGCAATAAAAAAGGTGCAGAGAAGTTTGTCAATGCTATTTTACGTCAATTCACCAGCCATC
CTCTCCCAGATATGGAAACAATTAAGCGTCGAAATAAGTATTATTCGGTCAAGTATTCACTGCCTGTTTGGTTGGTTAAA
AAGTTAGAAGACCAATTTGGCTCCGACAGAGCAGTGGCTATTATGGAGAGTTTATTTGTGCGTAGTAAGGCTAGTATTCG
CGTGACGGACCCTTTGAAGTTAGAAGAAGTTGCTGAGGCTTTAGATGCAGAACGTTCCCTTTTATCAGCAACTGGTTTAA
CCAAAGCGTCAGGTCATTTTGCAGCCAGTGATTATTTCACAAATGGGGACATCACTATTCAAGATGAAAGCAGTCAGCTT
GTAGCTCCTACTTTAAACATTGACGGTGACGACATAATTCTTGATGCGTGTAGCGCTCCAGGGGGTAAGACCTCTCATAT
AGCATCTTACTTAAAAACAGGTAAAGTTATTGCCTTAGATCTCTATGATCATAAACTAGAGTTGGTGAAGGAAAATGCTA
ACCGCTTAGGAGTTGCTGATAACATTGAGACAAGAAAACTAGATGCCAGAGAGGTTCATCGTCATTTTGAAAAGGATAGC
TTTGATAAGATTTTGGTGGATGCTCCTTGTTCAGGAATTGGTTTGATTCGTCGAAAACCCGATATCAAATATAATAAAGA
AAGTCAAGATTTTAATGCTCTACAGGCTATCCAGCTAGAGATATTGTCTAGTGTTTGTCAAACCTTGCGTAAAGGTGGTA
TAATAACATACAGTACTTGCACTATTTTTGATGAGGAGAATCGTCAAGTCATCGAAGCATTTTTACAGAGTCATCCTAAT
TTTGAACAAGTAAAACTGAACCATACACAAGCAGATATTATCAAGGATGGTTATCTTATTATCACACCAGAACAATACCA
GACTGATGGCTTCTTTATTGGACAAGTCAGACGAGTCTTGTAG

Upstream 100 bases:

>100_bases
GCTTTGTCCTTGATCGTTGTGCAACCGGCTGGTAAACCTAAAATGTCAATAATAGACTTTTTAAACGGCATTGGTAGAAA
ACTTGAAGTAGGGGACATCA

Downstream 100 bases:

>100_bases
TATTTTAGGAGGAAACTGACTGAGTCAGAAAAAACGTATGAAGATTTCATTAAAAACAGACATTGGGCAAAAACGCTCAA
ACAATCAAGATTTTATCAAT

Product: 16S rRNA m(5)C 967 methyltransferase

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKDRALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWV
YYLLMLSLYQLIYLDKLPAHAIVNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
KLEDQFGSDRAVAIMESLFVRSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASGHFAASDYFTNGDITIQDESSQL
VAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKVIALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDS
FDKILVDAPCSGIGLIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIEAFLQSHPN
FEQVKLNHTQADIIKDGYLIITPEQYQTDGFFIGQVRRVL

Sequences:

>Translated_440_residues
MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKDRALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWV
YYLLMLSLYQLIYLDKLPAHAIVNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
KLEDQFGSDRAVAIMESLFVRSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASGHFAASDYFTNGDITIQDESSQL
VAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKVIALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDS
FDKILVDAPCSGIGLIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIEAFLQSHPN
FEQVKLNHTQADIIKDGYLIITPEQYQTDGFFIGQVRRVL
>Mature_439_residues
ADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKDRALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVY
YLLMLSLYQLIYLDKLPAHAIVNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVKK
LEDQFGSDRAVAIMESLFVRSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASGHFAASDYFTNGDITIQDESSQLV
APTLNIDGDDIILDACSAPGGKTSHIASYLKTGKVIALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSF
DKILVDAPCSGIGLIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIEAFLQSHPNF
EQVKLNHTQADIIKDGYLIITPEQYQTDGFFIGQVRRVL

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=330, Percent_Identity=31.5151515151515, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI76150623, Length=330, Percent_Identity=31.5151515151515, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI32698918, Length=181, Percent_Identity=36.4640883977901, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI39995082, Length=230, Percent_Identity=30, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI23199998, Length=236, Percent_Identity=27.9661016949153, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI8922322, Length=236, Percent_Identity=27.9661016949153, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI301336155, Length=241, Percent_Identity=29.4605809128631, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI270288816, Length=236, Percent_Identity=27.9661016949153, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI270288818, Length=236, Percent_Identity=27.9661016949153, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI11545785, Length=159, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI2367212, Length=463, Percent_Identity=29.8056155507559, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081985, Length=220, Percent_Identity=30.9090909090909, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17536757, Length=239, Percent_Identity=32.2175732217573, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI115534021, Length=225, Percent_Identity=29.7777777777778, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71998419, Length=225, Percent_Identity=29.7777777777778, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25143471, Length=182, Percent_Identity=30.2197802197802, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=239, Percent_Identity=31.7991631799163, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319447, Length=183, Percent_Identity=29.5081967213115, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324306, Length=207, Percent_Identity=27.536231884058, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI22024126, Length=239, Percent_Identity=30.9623430962343, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24668781, Length=182, Percent_Identity=31.3186813186813, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21355201, Length=193, Percent_Identity=31.6062176165803, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356513, Length=178, Percent_Identity=32.0224719101124, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356579, Length=223, Percent_Identity=28.2511210762332, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 49623; Mature: 49492

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKDRALLTEIVYGTVSRKI
CCCCCCCCCCCCEEEEEEEHHCCCCCEEEEECHHHHHHHHCHHHHHHHHHHHHHHHHHEE
SLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLIYLDKLPAHAIVNDAVGIAKNRGNKKGAE
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHH
KFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVKKLEDQFGSDRAVAIMESLFV
HHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHH
RSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASGHFAASDYFTNGDITIQDESSQL
HCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCEEEEEECCCEE
VAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKVIALDLYDHKLELVKENANRLGVAD
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCCCCCC
NIETRKLDAREVHRHFEKDSFDKILVDAPCSGIGLIRRKPDIKYNKESQDFNALQAIQLE
CCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHH
ILSSVCQTLRKGGIITYSTCTIFDEENRQVIEAFLQSHPNFEQVKLNHTQADIIKDGYLI
HHHHHHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHCCCCHHEEEECCCHHHHHCCCEEE
ITPEQYQTDGFFIGQVRRVL
ECCCCCCCCCEEHHHHHHCC
>Mature Secondary Structure 
ADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKDRALLTEIVYGTVSRKI
CCCCCCCCCCCEEEEEEEHHCCCCCEEEEECHHHHHHHHCHHHHHHHHHHHHHHHHHEE
SLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLIYLDKLPAHAIVNDAVGIAKNRGNKKGAE
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHH
KFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVKKLEDQFGSDRAVAIMESLFV
HHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHH
RSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASGHFAASDYFTNGDITIQDESSQL
HCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCEEEEEECCCEE
VAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKVIALDLYDHKLELVKENANRLGVAD
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCCCCCC
NIETRKLDAREVHRHFEKDSFDKILVDAPCSGIGLIRRKPDIKYNKESQDFNALQAIQLE
CCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHH
ILSSVCQTLRKGGIITYSTCTIFDEENRQVIEAFLQSHPNFEQVKLNHTQADIIKDGYLI
HHHHHHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHCCCCHHEEEECCCHHHHHCCCEEE
ITPEQYQTDGFFIGQVRRVL
ECCCCCCCCCEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]