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The map label for this gene is liaS [H]

Identifier: 19746548

GI number: 19746548

Start: 1356920

End: 1357924

Strand: Reverse

Name: liaS [H]

Synonym: spyM18_1631

Alternate gene names: 19746548

Gene position: 1357924-1356920 (Counterclockwise)

Preceding gene: 19746549

Following gene: 19746547

Centisome position: 71.66

GC content: 34.53

Gene sequence:

>1005_bases
ATGAAAAAACGTTACTATGCTCTTGTTTGGCTCTACTCAACCATTACCATTTTATCTATTGTTTTTGTGGTCATGGATAA
TTTAGGAATCACTTTTAACTACCTTCGCAATCATTTATGGCAGGTCGAACGTCTAGGGTTTTCGATTTTATTGTTGATTG
TTTCTGTGACTTTATTATTGTTGTTATTGTGGATTATTATGGATGATAACAGTAAGCGTAACATCAACCAAAATCTAAAA
TATATTCTCAATAATCGACGACTCTATCTAGATGAGACATCAGAAATCAATACTAATTTAAGTCGACTTTCTAAAAAAAT
GTCTCACCTGACTGCTAACATGCAAAAGAAAGAAAGTGCTTATATTCTTGATAGCCAAGAAGTTGTAAAACAAGAGCGCA
AACGAATTGCAAGAGATTTACATGACACAGTCAGTCAAGAGCTATTCGCTTCGTCATTGATTTTATCAGGAATTTCGATG
AGTTTGGAACAACTGGACAAAACACAATTACAAACACAGTTAACAACGGTTGAAGCAATGTTGCAAAATGCTCAAAATGA
TCTACGCATTCTCCTTTTGCATCTTAGACCTACCGAGCTAGCTAATCGGACTTTATCTGAGGGCCTTAATATGATTCTTA
AGGAATTAACAGATAAAAGTGATATTGAAGTCATTTATAAGGAAACCATTGCTCAGCTTCCTAAAACAATGGAAGATAAT
CTTTTTAGAATTGCCCAAGAATTCATTAGCAACACGTTAAAACATGCTAAAGCTAGTCGAATTGAAGTTTATCTCAATCA
AACCTCAACAGAATTACAATTGAAGATGATTGATGATGGTGTAGGATTTGATATGGATCAGGTAAGGGATTTGAGTTATG
GTCTGAAGAATATTGAAGACCGTGTCAATGATTTAGCAGGAAACCTACATTTAATTAGTCAAAAAGGCAAAGGAGTTTCC
ATGGATATTAGACTGCCGATAGTGAAGGGAGATGACGATGAGTAA

Upstream 100 bases:

>100_bases
GCAACGAATCTATTAAGTTAAAAGAAACGGATAACCAATCCCTTAAAAAAGTCAAAATTATCGTTACAACTATTGCCGGA
AATGTTGAGGTGGATAGGCG

Downstream 100 bases:

>100_bases
GATAAAAGTGATATTGGTCGATGATCATGAAATGGTCCGCATGGGACTCAAGAGTTTTTTGAATTTACAAGCTGATATTG
ATGTCGTTGGTGAAGCCTCT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVERLGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLK
YILNNRRLYLDETSEINTNLSRLSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLNMILKELTDKSDIEVIYKETIAQLPKTMEDN
LFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKMIDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVS
MDIRLPIVKGDDDE

Sequences:

>Translated_334_residues
MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVERLGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLK
YILNNRRLYLDETSEINTNLSRLSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLNMILKELTDKSDIEVIYKETIAQLPKTMEDN
LFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKMIDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVS
MDIRLPIVKGDDDE
>Mature_334_residues
MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVERLGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLK
YILNNRRLYLDETSEINTNLSRLSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLNMILKELTDKSDIEVIYKETIAQLPKTMEDN
LFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKMIDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVS
MDIRLPIVKGDDDE

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=259, Percent_Identity=25.4826254826255, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38532; Mature: 38532

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVERLGFSILLLIVSVTLLL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSRLSKKMSHLTANMQKKESA
HHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC
YILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISMSLEQLDKTQLQTQLTTVEAM
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQNAQNDLRILLLHLRPTELANRTLSEGLNMILKELTDKSDIEVIYKETIAQLPKTMEDN
HHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHH
LFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKMIDDGVGFDMDQVRDLSYGLKNIED
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
RVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
HHHHHHCCEEEEECCCCCEEEEEEEEEECCCCCC
>Mature Secondary Structure
MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVERLGFSILLLIVSVTLLL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSRLSKKMSHLTANMQKKESA
HHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC
YILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISMSLEQLDKTQLQTQLTTVEAM
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQNAQNDLRILLLHLRPTELANRTLSEGLNMILKELTDKSDIEVIYKETIAQLPKTMEDN
HHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHH
LFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKMIDDGVGFDMDQVRDLSYGLKNIED
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
RVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
HHHHHHCCEEEEECCCCCEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]