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The map label for this gene is ytzG [H]

Identifier: 19746361

GI number: 19746361

Start: 1177296

End: 1178060

Strand: Reverse

Name: ytzG [H]

Synonym: spyM18_1406

Alternate gene names: 19746361

Gene position: 1178060-1177296 (Counterclockwise)

Preceding gene: 19746362

Following gene: 19746360

Centisome position: 62.17

GC content: 41.18

Gene sequence:

>765_bases
GTGACTTCTATTTCTTTTGCAAGGGTAAATTTATGTTACACTACTAATATGCGTTTAGATAAATTATTAGAAGGTACAAA
AGTTGGCTCTAGAAGTCAAGTGAAGAAACTGATTAAGGCTCAAGGGGTTTGGGTTGATCACATGCCTGCCAGAAATGGTC
GACAAAATGTTGATCCTGGCTTGCAACTGATTGAGGTGACTGGACAGCGAGTGACGCATCCTAAACATTCCTACATTATC
CTCAACAAGCCATCAGGGGTGGTCTCAGCTAAGAAAGATACTAACTATTTAACCATCATTGATCAGCTTGCTGAAGAAGA
TAAAAGTCCAGACTTGTATCCTGTTGGCAGATTGGATAGGGATACCGAAGGGTTGGTTTTACTCACTGATAATGGTCCCC
TAGGGTTTCGGATGTTGCACCCTAGTCATCATGTTTCTAAAACGTATCTTGTAACCGTTAATGGCTTGTTAGCAGAAGAT
GCCTCAGATTTTTTTGCAGCTGGTATTTGTTTCCCAACAGGTGAACAATGCCAACCAGCTCAATTGACTATTCTTAAAGC
CGACACAGACCAGTCACAGGCTAGTTTGACCATTTCAGAAGGAAAATTTCACCAAGTGAAAAAAATGTTTTTGACCTATG
GTTTGAAAGTGACCTCTCTGAAACGGACGCACTTTGCAGGACTGGAACTCGGTCATTTAGCCAGCGGAGAATATCGTTAT
CTAACAGAGTCAGAAAGGCAACTCATTAAAACGTATCTAGACTGA

Upstream 100 bases:

>100_bases
GATGAAGCTGCTGCGTCACAATTAGACTAACCTAACCAGTCGTTAAACCTTGTAAGAACAAAAATGAGGAGATAGTCAAC
CTCATTTAAGAAGAAGTCTT

Downstream 100 bases:

>100_bases
GCAAAACATGCTTTCAAAAAGAAGCTATTCTTGCTTAATCAATAAAGTGTCCATTGACTATTATAGTCATGATGAGTCCG
ACTTTTGCTATGTTTAGTCA

Product: 16S pseudouridylate synthetase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTSISFARVNLCYTTNMRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYII
LNKPSGVVSAKKDTNYLTIIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAED
ASDFFAAGICFPTGEQCQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRY
LTESERQLIKTYLD

Sequences:

>Translated_254_residues
MTSISFARVNLCYTTNMRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYII
LNKPSGVVSAKKDTNYLTIIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAED
ASDFFAAGICFPTGEQCQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRY
LTESERQLIKTYLD
>Mature_253_residues
TSISFARVNLCYTTNMRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIIL
NKPSGVVSAKKDTNYLTIIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDA
SDFFAAGICFPTGEQCQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRYL
TESERQLIKTYLD

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=37.3949579831933, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790453, Length=239, Percent_Identity=29.7071129707113, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787524, Length=223, Percent_Identity=28.2511210762332, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081838, Length=201, Percent_Identity=28.3582089552239, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 28291; Mature: 28160

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSISFARVNLCYTTNMRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
CCCCEEEEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCC
LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTIIDQLAEEDKSPDLYPVGRLDR
CEEEEECCCCCCCCCCCEEEEECCCCCEEECCCCCEEEEHHHHHHHCCCCCEEECCCCCC
DTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQCQPA
CCCCEEEEECCCCEEEEEECCCCCCCEEEEEEECCEECCCCHHHHEEEEECCCCCCCCCC
QLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRY
EEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCEEEEECCHHCCCCEECCCCCCCEEE
LTESERQLIKTYLD
ECHHHHHHHHHHCC
>Mature Secondary Structure 
TSISFARVNLCYTTNMRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
CCCEEEEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCC
LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTIIDQLAEEDKSPDLYPVGRLDR
CEEEEECCCCCCCCCCCEEEEECCCCCEEECCCCCEEEEHHHHHHHCCCCCEEECCCCCC
DTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQCQPA
CCCCEEEEECCCCEEEEEECCCCCCCEEEEEEECCEECCCCHHHHEEEEECCCCCCCCCC
QLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLASGEYRY
EEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCEEEEECCHHCCCCEECCCCCCCEEE
LTESERQLIKTYLD
ECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]