Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 19746195

GI number: 19746195

Start: 1019590

End: 1021908

Strand: Reverse

Name: pcrA [H]

Synonym: spyM18_1217

Alternate gene names: 19746195

Gene position: 1021908-1019590 (Counterclockwise)

Preceding gene: 19746198

Following gene: 19746194

Centisome position: 53.93

GC content: 41.05

Gene sequence:

>2319_bases
ATGAATCCTTTATTAAATGGAATGAATGATCGGCAGGCACAAGCTGTTCAAACAACTGAAGGTCCCTTATTGATTATGGC
GGGGGCTGGTTCTGGTAAAACAAGGGTACTGACCCATCGTATTGCCTATTTAATTGATGAAAAATTTGTCAATCCTTGGA
ATATTTTAGCTATTACCTTTACTAATAAGGCTGCGCGTGAAATGAAAGAAAGAGCCTTGGCCTTAAATCCAGCTACTAAA
GATACCTTAATTGCTACCTTTCACTCCATGTGTGTACGGATTCTGCGTCGTGAGGCTGATCACATTGGTTACAACCGAAA
CTTCACAATCGTGGATCCAGGTGAGCAACGAACGTTGATGAAGCGCATTTTAAAACAATTAAATATAGACCCTAAAAAGT
GGAATGAACGCTCTATTTTAGGAACTATTTCAAACGCTAAAAATGATTTGCTTGATGAAAAGGGATACGAGGCACAGGCT
GCTGATATGTATAGTCAAATCGTAGCGAGGTGTTACAAAGCTTATCAGGAAGAATTACGACGTAGCGAAGCACTTGACTT
TGATGATTTAATCATGATGACGCTACGCCTGTTTGACGCTAATCCTGACGTGTTAGCTTATTACCAGCAGCGTTACCAAT
ACATTCATGTGGACGAGTATCAAGATACCAACCATGCCCAATATCAATTAGTCAAGTTACTAGCTTCTCGCTTTAAAAAT
ATTTGCGTTGTTGGAGACGCTGATCAGTCTATCTATGGTTGGCGGGGAGCTGACATGCAAAATATTCTTGATTTTGAAAA
AGATTACCCAGATGCTAAGGTAGTGCTTTTAGAAGAAAACTATCGCTCTACCAAGAAAATTCTGCAAGCAGCTAACGACG
TGATTAACAATAACCGTAATCGCCGTCCTAAGAAACTTTGGACACAAAATGCGGATGGGGAACAACTGGTTTATTACAGG
GCAAATGATGAGCGAGATGAGGCAGTATTTGTGGCCTCAACCATTAGCAATATGAGTCAAGAGCTTGGAAAAAACTTCAA
AGATTTCGCTGTTTTATACCGTACCAATGCCCAATCTCGGACCATTGAAGAAGCACTCTTAAAATCCAATATTCCTTACA
CCATGGTTGGAGGCACCAAGTTTTACAGCCGTAAAGAAATTCGGGATTTGATTGCCTATTTAACCATTGTTGCTAACCCT
GCTGATAATATTTCTTTTGAGCGGATTGTGAATGAGCCTAAACGAGGTGTTGGTCCAGGAACGCTTGATAAACTGCGTCA
GTTTGCCTATGAGAGTGACCAGTCTTTATTAGAAGCAGCTTCAAATCTATTGATGTCCCCCTTGAAAGGTAAAGCAGCAC
AAGCTATCATGGACTTGGCCAATATTCTCGGTCAGCTACGTCAAGACCTAGATCAGATGAGTATCACTGACTTAGCAGAA
GCTTTGTTAGAAAAAACAGGTTATTTAGATAGCTTACGCCTGCAAAATACATTGGAAAGCCAAGCCCGTATCGAAAATAT
CGAAGAATTCTTATCTGTTACTAAAAATTTTGACGAGTCAAGTGCCTCTCAAGAAGAAGACGAAACCGGTGTTGATCGCT
TGGGACGTTTTTTAAACGATTTAGCACTGATTGCTGATACAGATGATTCCCAAGCAGAAGCTTCTGAAGTAACCTTAATG
ACCCTACATGCTGCCAAAGGCTTGGAGTTCCCTGTTGTTTTCTTAATTGGTATGGAAGAAGGTGTCTTCCCCTTATCACG
TGCCTCTGAAGATCCAGATGAATTGGAAGAAGAAAGACGTTTAGCTTATGTCGGAATCACAAGAGCTGAAGAAGTTCTCT
TTATGACCAATGCCAACACCAGAACCCTCTTTGGTAAAAGTAGTTATAACCGTCCAACACGCTTCTTGAAAGAAATTTCA
GAGGAACTACTCTCTTATAAAGGATTGGCAAGACCAGCACAATCTTCATTTGGTGTGCGTTTTTCTACTGAAACACACAC
ACAGTTTGGACAAGGGATGAGTCTATCAGAAGCCTTACAAGCTCGAAAGGCCCAGGCTCAAGTAAGACAATCAGCACAGC
CAATGCAGGCACATACGATACCATCAGCTTCAACTAGCTCAGTTTTACCGCTTGGTTCTAATAGCTCTGTAGAAGAGGTC
ACTTGGCAGATTGGCGATATCGCTCACCATAAAAAATGGGGAGACGGGACTGTGCTTGAGGTTTCAGGCTCCGGAAAAAC
GATGGAATTGAAAATCAAATTTCCAGAAGTTGGTCTAAAGAAATTACTAGCAAGTGTTGCTCCTATTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
CTCTTTACTTTTGGGGTCTTCTTTGCTATTTCCCTTGGAAACATGATATAATTTTAAGGTTGATAAAAGAGATGTCACTG
AACGAGATTAAGGAGAAAGC

Downstream 100 bases:

>100_bases
GAAAAAAATGAGCTTCCTACTATATAAGTGAGTAGGAAGCTCATTTTTTTAGATTTCTGTATCATATAAAAGTTAAAAAA
TTTTAAGTTTTAGGAGCATA

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 772; Mature: 772

Protein sequence:

>772_residues
MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKFVNPWNILAITFTNKAAREMKERALALNPATK
DTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVDEYQDTNHAQYQLVKLLASRFKN
ICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVLLEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYR
ANDERDEAVFVASTISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIAYLTIVANP
ADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKGKAAQAIMDLANILGQLRQDLDQMSITDLAE
ALLEKTGYLDSLRLQNTLESQARIENIEEFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEASEVTLM
TLHAAKGLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLFGKSSYNRPTRFLKEIS
EELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQARKAQAQVRQSAQPMQAHTIPSASTSSVLPLGSNSSVEEV
TWQIGDIAHHKKWGDGTVLEVSGSGKTMELKIKFPEVGLKKLLASVAPIEKK

Sequences:

>Translated_772_residues
MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKFVNPWNILAITFTNKAAREMKERALALNPATK
DTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVDEYQDTNHAQYQLVKLLASRFKN
ICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVLLEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYR
ANDERDEAVFVASTISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIAYLTIVANP
ADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKGKAAQAIMDLANILGQLRQDLDQMSITDLAE
ALLEKTGYLDSLRLQNTLESQARIENIEEFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEASEVTLM
TLHAAKGLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLFGKSSYNRPTRFLKEIS
EELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQARKAQAQVRQSAQPMQAHTIPSASTSSVLPLGSNSSVEEV
TWQIGDIAHHKKWGDGTVLEVSGSGKTMELKIKFPEVGLKKLLASVAPIEKK
>Mature_772_residues
MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKFVNPWNILAITFTNKAAREMKERALALNPATK
DTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVDEYQDTNHAQYQLVKLLASRFKN
ICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVLLEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYR
ANDERDEAVFVASTISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIAYLTIVANP
ADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKGKAAQAIMDLANILGQLRQDLDQMSITDLAE
ALLEKTGYLDSLRLQNTLESQARIENIEEFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEASEVTLM
TLHAAKGLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLFGKSSYNRPTRFLKEIS
EELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQARKAQAQVRQSAQPMQAHTIPSASTSSVLPLGSNSSVEEV
TWQIGDIAHHKKWGDGTVLEVSGSGKTMELKIKFPEVGLKKLLASVAPIEKK

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=776, Percent_Identity=38.5309278350515, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI48994965, Length=653, Percent_Identity=40.1225114854518, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1787196, Length=347, Percent_Identity=26.2247838616715, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322369, Length=730, Percent_Identity=29.041095890411, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6324477, Length=673, Percent_Identity=22.8826151560178, Blast_Score=117, Evalue=9e-27,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 87000; Mature: 87000

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKFVNPWNILAITF
CCCCCCCCCCHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEE
TNKAAREMKERALALNPATKDTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLM
CCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
KRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQAADMYSQIVARCYKAYQEELR
HHHHHHCCCCCCCCCCCHHEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVDEYQDTNHAQYQLVKLLASRFKN
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCC
ICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVLLEENYRSTKKILQAANDVINNNRN
EEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC
RRPKKLWTQNADGEQLVYYRANDERDEAVFVASTISNMSQELGKNFKDFAVLYRTNAQSR
CCCHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCHHHHEEEEECCCCHH
TIEEALLKSNIPYTMVGGTKFYSRKEIRDLIAYLTIVANPADNISFERIVNEPKRGVGPG
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCC
TLDKLRQFAYESDQSLLEAASNLLMSPLKGKAAQAIMDLANILGQLRQDLDQMSITDLAE
HHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLEKTGYLDSLRLQNTLESQARIENIEEFLSVTKNFDESSASQEEDETGVDRLGRFLND
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LALIADTDDSQAEASEVTLMTLHAAKGLEFPVVFLIGMEEGVFPLSRASEDPDELEEERR
HEEEECCCCCCCHHHHEEEEEEEHHCCCCCCEEEEEECCCCCCCHHCCCCCHHHHHHHHC
LAYVGITRAEEVLFMTNANTRTLFGKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVR
EEEEEECCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
FSTETHTQFGQGMSLSEALQARKAQAQVRQSAQPMQAHTIPSASTSSVLPLGSNSSVEEV
EECCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEECCCCCCHHHH
TWQIGDIAHHKKWGDGTVLEVSGSGKTMELKIKFPEVGLKKLLASVAPIEKK
HHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKFVNPWNILAITF
CCCCCCCCCCHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEE
TNKAAREMKERALALNPATKDTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLM
CCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
KRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQAADMYSQIVARCYKAYQEELR
HHHHHHCCCCCCCCCCCHHEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVDEYQDTNHAQYQLVKLLASRFKN
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCC
ICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVLLEENYRSTKKILQAANDVINNNRN
EEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC
RRPKKLWTQNADGEQLVYYRANDERDEAVFVASTISNMSQELGKNFKDFAVLYRTNAQSR
CCCHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCHHHHEEEEECCCCHH
TIEEALLKSNIPYTMVGGTKFYSRKEIRDLIAYLTIVANPADNISFERIVNEPKRGVGPG
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCC
TLDKLRQFAYESDQSLLEAASNLLMSPLKGKAAQAIMDLANILGQLRQDLDQMSITDLAE
HHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLEKTGYLDSLRLQNTLESQARIENIEEFLSVTKNFDESSASQEEDETGVDRLGRFLND
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LALIADTDDSQAEASEVTLMTLHAAKGLEFPVVFLIGMEEGVFPLSRASEDPDELEEERR
HEEEECCCCCCCHHHHEEEEEEEHHCCCCCCEEEEEECCCCCCCHHCCCCCHHHHHHHHC
LAYVGITRAEEVLFMTNANTRTLFGKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVR
EEEEEECCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
FSTETHTQFGQGMSLSEALQARKAQAQVRQSAQPMQAHTIPSASTSSVLPLGSNSSVEEV
EECCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEECCCCCCHHHH
TWQIGDIAHHKKWGDGTVLEVSGSGKTMELKIKFPEVGLKKLLASVAPIEKK
HHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA