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The map label for this gene is ciaH [H]

Identifier: 19746166

GI number: 19746166

Start: 993806

End: 995116

Strand: Reverse

Name: ciaH [H]

Synonym: spyM18_1186

Alternate gene names: 19746166

Gene position: 995116-993806 (Counterclockwise)

Preceding gene: 19746167

Following gene: 19746163

Centisome position: 52.51

GC content: 31.58

Gene sequence:

>1311_bases
ATGAATAAATTAAAAAAAGAGATTTTATCAGATAACTATAACCACTTTTTTCATTTTTTTGCGGTTTTTACAGGTATTTT
TGTCATTATGACTATTATTATCTTACAGATTATGCGGTTTGGCGTTTATTCGTCAGTTGACAGTAGTTTAGTTTCTGTTA
GTAATAATGCAAGTAGCTATGCTAATCGTACGATGGCTAGAATATCTTCTTTTTACTTTGATACTGAAAATAACATTATT
AAGGCGCTGCCTGATTCGGATAGTTCTAAGTTATTAGGAACGCCTGCAGCTAATACAGATATCATTTTGTTTAGTGCTAA
TGGAACAATTTTAAATGCTTTTGATGCGTTTTCTAACTATCAAAATTTTCATTTAGATAAACGCCGGTTGGGGAGTATTG
AAACCACCAGTTTAATAAATTTTTATGGACAAGAAGAAAAATACCATACGATAACTGTAAGGGTTCATATCAAAAATTAT
CCTGCAGTTGCCTATATGATGGCAGTAGTAAATGTGGAACAATTAGACCGCGCTAATGAACGTTACGAGCGCATTATTAT
TATAGTTATGAGTGTTTTTTGGCTAATTTCTATTTTAGCAAGTATTTATTTAGCCAAGTGGAGCAGAAAACCTATTTTAG
AAAGCTATGAAAAACAAAAAATGTTTGTTGAAAATGCTAGTCATGAATTAAGGACCCCTTTGGCGGTCTTACAGAATCGT
CTGGAATCGCTTTTTCGTAAGCCCAACGAAACGATATTAGAAAATAGTGAGCATCTCGCTTCTAGTTTAGACGAGGTTCG
CAACATGCGCATCTTAACAACTAATTTATTAAATTTAGCAAGACGAGATGATGGCATTAATCCACAGTGGACTCATTTAG
ATACAGATTTTTTTAATGCTATTTTTGAGAATTATGAACTAGTTGCTAAAGAATATGGAAAAATATTTTATTTTCAGAAC
CAAGTCAATAGATCGTTAAGAATGGATAAGGCTTTACTAAAACAATTAATAACGATTTTATTTGACAATGCTATTAAATA
TACAGATAAAAATGGTATTATTGAAATTATAGTGAAAACAACGGACAAAAATTTATTAATTTCTGTTATTGATAATGGTC
CAGGGATAACAGATGAAGAAAAGAAAAAGATTTTTGATCGTTTTTATCGAGTTGACAAAGCTAGAACACGGCAAACAGGT
GGATTTGGCTTGGGGTTGGCTTTAGCTCAGCAAATCGTGATGTCTTTAAAAGGAAATATTACAGTAAAGGATAATGATCC
TAAAGGTAGTATTTTTGAAGTCAAACTATAA

Upstream 100 bases:

>100_bases
GTTGAAGTTTACATTTCTAAAATAAGGAAAAAATTAAAAGGTACCCGTTTTGTTAACAGGCTTCAAACTTTAAGAAGCGT
AGGGTATATTCTAAAAAACA

Downstream 100 bases:

>100_bases
GCTAAAAAGGGCGACCAATAAGGTCAGCCCTTTTAAAGTTATTTTTAACTGTCAACGCTTTATTGTATTTTTTGAGTTTT
TACCGTATAAAAAAACTCCA

Product: histidine kinase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYSSVDSSLVSVSNNASSYANRTMARISSFYFDTENNII
KALPDSDSSKLLGTPAANTDIILFSANGTILNAFDAFSNYQNFHLDKRRLGSIETTSLINFYGQEEKYHTITVRVHIKNY
PAVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEKQKMFVENASHELRTPLAVLQNR
LESLFRKPNETILENSEHLASSLDEVRNMRILTTNLLNLARRDDGINPQWTHLDTDFFNAIFENYELVAKEYGKIFYFQN
QVNRSLRMDKALLKQLITILFDNAIKYTDKNGIIEIIVKTTDKNLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTG
GFGLGLALAQQIVMSLKGNITVKDNDPKGSIFEVKL

Sequences:

>Translated_436_residues
MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYSSVDSSLVSVSNNASSYANRTMARISSFYFDTENNII
KALPDSDSSKLLGTPAANTDIILFSANGTILNAFDAFSNYQNFHLDKRRLGSIETTSLINFYGQEEKYHTITVRVHIKNY
PAVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEKQKMFVENASHELRTPLAVLQNR
LESLFRKPNETILENSEHLASSLDEVRNMRILTTNLLNLARRDDGINPQWTHLDTDFFNAIFENYELVAKEYGKIFYFQN
QVNRSLRMDKALLKQLITILFDNAIKYTDKNGIIEIIVKTTDKNLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTG
GFGLGLALAQQIVMSLKGNITVKDNDPKGSIFEVKL
>Mature_436_residues
MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYSSVDSSLVSVSNNASSYANRTMARISSFYFDTENNII
KALPDSDSSKLLGTPAANTDIILFSANGTILNAFDAFSNYQNFHLDKRRLGSIETTSLINFYGQEEKYHTITVRVHIKNY
PAVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEKQKMFVENASHELRTPLAVLQNR
LESLFRKPNETILENSEHLASSLDEVRNMRILTTNLLNLARRDDGINPQWTHLDTDFFNAIFENYELVAKEYGKIFYFQN
QVNRSLRMDKALLKQLITILFDNAIKYTDKNGIIEIIVKTTDKNLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTG
GFGLGLALAQQIVMSLKGNITVKDNDPKGSIFEVKL

Specific function: Member of the two-component regulatory system CiaH/CiaR. Involved in early steps of competence regulation and in penicillin susceptibility. Probably phosphorylates CiaR [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=219, Percent_Identity=31.9634703196347, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786783, Length=230, Percent_Identity=30.8695652173913, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788393, Length=229, Percent_Identity=29.6943231441048, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=28.82096069869, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790346, Length=218, Percent_Identity=30.2752293577982, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787894, Length=225, Percent_Identity=28, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788279, Length=214, Percent_Identity=30.3738317757009, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=224, Percent_Identity=32.1428571428571, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790861, Length=226, Percent_Identity=26.5486725663717, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=27.9279279279279, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=239, Percent_Identity=26.7782426778243, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788713, Length=226, Percent_Identity=21.6814159292035, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082128, Length=228, Percent_Identity=25.4385964912281, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790436, Length=338, Percent_Identity=24.2603550295858, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789808, Length=221, Percent_Identity=25.7918552036199, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI48994928, Length=226, Percent_Identity=29.2035398230088, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50095; Mature: 50095

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYSSVDSSLVSVSNNASSY
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ANRTMARISSFYFDTENNIIKALPDSDSSKLLGTPAANTDIILFSANGTILNAFDAFSNY
HHHHHHHHHHHHEECCCCEEEECCCCCCCCEEECCCCCCEEEEEECCCCEEEHHHHHCCC
QNFHLDKRRLGSIETTSLINFYGQEEKYHTITVRVHIKNYPAVAYMMAVVNVEQLDRANE
CCCCCCHHHCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEKQKMFVENASHELRTPLAVLQNR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCHHHHHHHH
LESLFRKPNETILENSEHLASSLDEVRNMRILTTNLLNLARRDDGINPQWTHLDTDFFNA
HHHHHHCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHH
IFENYELVAKEYGKIFYFQNQVNRSLRMDKALLKQLITILFDNAIKYTDKNGIIEIIVKT
HHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEEEE
TDKNLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTGGFGLGLALAQQIVMSLKGNI
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCE
TVKDNDPKGSIFEVKL
EEECCCCCCCEEEEEC
>Mature Secondary Structure
MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYSSVDSSLVSVSNNASSY
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ANRTMARISSFYFDTENNIIKALPDSDSSKLLGTPAANTDIILFSANGTILNAFDAFSNY
HHHHHHHHHHHHEECCCCEEEECCCCCCCCEEECCCCCCEEEEEECCCCEEEHHHHHCCC
QNFHLDKRRLGSIETTSLINFYGQEEKYHTITVRVHIKNYPAVAYMMAVVNVEQLDRANE
CCCCCCHHHCCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEKQKMFVENASHELRTPLAVLQNR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCHHHHHHHH
LESLFRKPNETILENSEHLASSLDEVRNMRILTTNLLNLARRDDGINPQWTHLDTDFFNA
HHHHHHCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHH
IFENYELVAKEYGKIFYFQNQVNRSLRMDKALLKQLITILFDNAIKYTDKNGIIEIIVKT
HHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEEEE
TDKNLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTGGFGLGLALAQQIVMSLKGNI
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCE
TVKDNDPKGSIFEVKL
EEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]