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The map label for this gene is folC [H]

Identifier: 19745894

GI number: 19745894

Start: 710381

End: 711649

Strand: Reverse

Name: folC [H]

Synonym: spyM18_0876

Alternate gene names: 19745894

Gene position: 711649-710381 (Counterclockwise)

Preceding gene: 19745895

Following gene: 19745888

Centisome position: 37.55

GC content: 40.66

Gene sequence:

>1269_bases
ATGACTACCTATGATAGCATTTCCTGGATTCATACCTTTAAGGCTAATGGTCGCAAGACCGATTTGGCCAGAATGGCTTG
GTTACTTGAGGCACTAGGCCGGCCACAAGACAAATTCCCTGCTATTCACGTTGTTGGAACTAACGGCAAGGGCTCTGTGA
CAGCCTACTTACAGCATATCCTGTCAACTTCTGGTTATCAGGTCGGAACCTTTACATCTCCTTTTATCATCTCTTTTCAC
GATCGGATCTGCCTTAATGGCCAGCCCATTTCAGACCTAGAATTGGCGCAATGTGTGAGCGTGATTAGACCCATTCTCAC
TAAAATGTCCCTTGAAACAAATTGGGATAGACCTACCGAGTTTGAGTTAGTGACTCTCATAATGTTTTGCTACTTTGCCA
ATCTTAAACCTGTTGATATTGCCATTATTGAGGCAGGGATTGGTGGGAAAACCGACGCAACCAATGTTTTTCATGCTCTG
GCCGTCGTATGCCCTTCCATTAGTTTTGATCACCAAGAACGTTTAGGTTATAGCCTAGCAGAAATTGCTCACCAAAAAGC
AGAAGTTATCAAAGCTAAAGAACCCGTTATTATCGGTCAACTTGAGCAGGAGGCTAGTCAAGTCTTTCAACAAGTTACCC
AAAAAGCCGGTTCACGCTTATATCAATTAGGAAAAGATTTTTTGCTCAAGCCTTCAGAAAAGACGTTTTCCTTTCAACAC
GATAACTTGAATTTAACCAAACTCCGTCTCAAACTATTAGGTCAACATCAAAAAGCGAATGCCTGTTTAGCCATTATGAC
AGCTCAATTACTTAGAAAATCCTTTCCTAAAATATCTCCAAAAACGATTCAAAAAGGAATTGAGACAACAACTTGGCCTG
GCCGGTTCGAATTTATTCAACCTAATCTTCTATTAGACGGCGCTCACAATCCAGATTCCATTGCTAAATTAAAGTCTCTA
CTGCAAGAAGAATTTCCCAAACGTCCTATTCATATCCTCTTTGCAGGTCTCAAACGTAAGCCTTTAGCCGACTTGCTGGC
TCAACTAGATACTTTTGAAGTATCTGTCACGACTTTTGATTTCCCTGAAGCAAATCTTTTAGAGGATTATCCTGATAAAT
ACCCACAGGTAAAAGATTACAGAGACTGGTTACAAGGTTCCGTAACATCTGATGAACTCTTTGTTGTTACAGGGTCACTT
TATTTTATCTCCGAGGTAAGGCAATATTGTCTGAGCAACGCAAATATCTTTAAAAACATATTCCCCTAA

Upstream 100 bases:

>100_bases
CTGAAGGGACGCAACTCCCTAATGCAACTGGAGCTGAAGATGCTAATACTGGTCTTCCTACGAACCCTTAGAAGCATTGT
TAGAAGGACAAGAAATAGTA

Downstream 100 bases:

>100_bases
TCACCTAACAAAAAACGATGCTAGCAGTTTAGAAGACTTTCCTCAAAAACTGTTAACATCATTTTTTATTGCCTTAGACT
ACATTTTTTAGCGCATGGTG

Product: folyl-polyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHVVGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFH
DRICLNGQPISDLELAQCVSVIRPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGSRLYQLGKDFLLKPSEKTFSFQH
DNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFPKISPKTIQKGIETTTWPGRFEFIQPNLLLDGAHNPDSIAKLKSL
LQEEFPKRPIHILFAGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDELFVVTGSL
YFISEVRQYCLSNANIFKNIFP

Sequences:

>Translated_422_residues
MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHVVGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFH
DRICLNGQPISDLELAQCVSVIRPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGSRLYQLGKDFLLKPSEKTFSFQH
DNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFPKISPKTIQKGIETTTWPGRFEFIQPNLLLDGAHNPDSIAKLKSL
LQEEFPKRPIHILFAGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDELFVVTGSL
YFISEVRQYCLSNANIFKNIFP
>Mature_421_residues
TTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHVVGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHD
RICLNGQPISDLELAQCVSVIRPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHALA
VVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGSRLYQLGKDFLLKPSEKTFSFQHD
NLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFPKISPKTIQKGIETTTWPGRFEFIQPNLLLDGAHNPDSIAKLKSLL
QEEFPKRPIHILFAGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDELFVVTGSLY
FISEVRQYCLSNANIFKNIFP

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=320, Percent_Identity=30.9375, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI66932990, Length=320, Percent_Identity=30.9375, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1788654, Length=311, Percent_Identity=29.903536977492, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17553148, Length=345, Percent_Identity=28.4057971014493, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=345, Percent_Identity=28.4057971014493, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17553150, Length=337, Percent_Identity=28.7833827893175, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=348, Percent_Identity=32.183908045977, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6324815, Length=326, Percent_Identity=27.3006134969325, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322718, Length=432, Percent_Identity=25.2314814814815, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24641571, Length=326, Percent_Identity=30.6748466257669, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24581568, Length=327, Percent_Identity=25.3822629969419, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47526; Mature: 47395

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHVVGTNGKGSVTAYLQHI
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHH
LSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVIRPILTKMSLETNWDRPTE
HHCCCCEEECCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
FELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHALAVVCPSISFDHQERLGYSLA
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCHHHH
EIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGSRLYQLGKDFLLKPSEKTFSFQH
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEC
DNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFPKISPKTIQKGIETTTWPGRFEFIQ
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEEEC
PNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILFAGLKRKPLADLLAQLDTFEVSVTTFD
CCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHCCEEEEEEEEC
FPEANLLEDYPDKYPQVKDYRDWLQGSVTSDELFVVTGSLYFISEVRQYCLSNANIFKNI
CCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHC
FP
CC
>Mature Secondary Structure 
TTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHVVGTNGKGSVTAYLQHI
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHH
LSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVIRPILTKMSLETNWDRPTE
HHCCCCEEECCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
FELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHALAVVCPSISFDHQERLGYSLA
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCHHHH
EIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGSRLYQLGKDFLLKPSEKTFSFQH
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEC
DNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFPKISPKTIQKGIETTTWPGRFEFIQ
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEEEC
PNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILFAGLKRKPLADLLAQLDTFEVSVTTFD
CCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHCCEEEEEEEEC
FPEANLLEDYPDKYPQVKDYRDWLQGSVTSDELFVVTGSLYFISEVRQYCLSNANIFKNI
CCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHC
FP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]