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The map label for this gene is lmrB [H]

Identifier: 19745688

GI number: 19745688

Start: 508819

End: 510186

Strand: Reverse

Name: lmrB [H]

Synonym: spyM18_0639

Alternate gene names: 19745688

Gene position: 510186-508819 (Counterclockwise)

Preceding gene: 19745692

Following gene: 19745684

Centisome position: 26.92

GC content: 38.89

Gene sequence:

>1368_bases
ATGTCACATCATCAACAAACCGTTTCAAAACAAACAATTATGGCGATTATCGCCATAGCACTCATTGGTTTTTCAGGAAT
TTTGTCTGAAACCAGTATGAATGTCACCTTCCCGACACTGATGTCAGTCTATCAGTTGCCCTTAAATAGCTTGCAATGGA
TGACGACCATTTATTTACTAGCAGTGGCGATTATGATGACCACTTCGGCTACACTGAAAAAAAATGTGCGGGAAAGACCC
CTCTTTTTTATGGCCACAGGTCTTTTTACATTTGGCACCATCTTGGCCGTTCTGACCCAGTCCTTTGCGATCATGTTGCT
AGCCCGCATTTTTCAAGGCATTGGTACTGGTCTGGTAATGCCTCAGATGTTTAATATTATTTTAGAGCGTGTCCCAATGC
ATAAGGTAGGTCTATTTATGGGATTTGCTGGTCTTATTATTAGCTTAGCACCTGCTTTTGGGCCTACTTATGGGGGCTTT
ATGATTAGCCATTTTAGTTGGCAATGGATTTTTATCTGTATACTCCCTGTACCACTGATTGCAGGTATTCTAGCTTATTA
TTACCTGGAAGATTCTCCAGTAAGCGAAAAAGTACCCTTTGACTGGTTGGCATTTATTGCACTATCGATTAGCTTAACTT
CTGCCTTATTAGCTATTACTAGTCTAGAAAACGGAAGTGTTAATTTGTATTACTTAGGGCTTTTTATTCTCAGCTTTGTC
CTCTTCCTCTACAAGAATCTCACAGCTAAGCAACCCTTTCTTGATATTCGCATTCTCAAAATTCCCTCTCTAACCTTTGG
CCTGATTCCCTTTTTTGTCTTCCAGCTGATTAATTTAGGCATAAATTTTCTAACGCCAAACTTTATTGTCATGGAAAAGA
TTGCTAATAGTTCTCAAGCTGGTATGGTGTTACTACCTGGTACCTTACTCGGAGCTCTACTAGCACCTGCTTTTGGTAAA
CTTTATGATCAAAAAGGAGCAAGACTTTCGCTTTATTTAGGAAATTCCTTATTTAGTTTATCATTGATTATTATGACACT
TCAAACAAGACATTTTATGCTTTTACCATTCACTCTTTTATATATTTTATTCACGTTTGGGCGTAACATGGGCTTTAATA
ATAGCTTAGCCACGGCTATTCGAGAATTGCCTGCCGAGAAAAATGCCGATGCCACGGCCATTTTTCAGATGATGCAGCAA
TTTGCTGGCGCTCTAGGAACTGCTATGGCATCACTGATAGCAAATAGTCAACCAGAATTCACAAGCGGTGTCCAGTCTGT
CTACCTTCTCTTTACTATTTTTGCTCTACTTGATTTTATCTTTTTCTTTGCTATGTTTTACCATTTAGGGAAAAAAGGAT
TAGCCTAA

Upstream 100 bases:

>100_bases
CAACTATTGTTATTCGTATCTATTGATTTAGCAAAAAAGAGTTTCTGACAATTTTAAGATTATATGATACAATAACAATC
GATTAGATAAAGGAGTTATC

Downstream 100 bases:

>100_bases
GATGACATACTAAAAACCAGTCTCGTTTTATTGAACGAGACTGGTTTTTGCTGTTATAAGATACCTTCCAAAAGGCCTTT
CATAAAGTCTTCTGAGTGGA

Product: drug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVYQLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERP
LFFMATGLFTFGTILAVLTQSFAIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLAITSLENGSVNLYYLGLFILSFV
LFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQLINLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGK
LYDQKGARLSLYLGNSLFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADATAIFQMMQQ
FAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLGKKGLA

Sequences:

>Translated_455_residues
MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVYQLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERP
LFFMATGLFTFGTILAVLTQSFAIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLAITSLENGSVNLYYLGLFILSFV
LFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQLINLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGK
LYDQKGARLSLYLGNSLFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADATAIFQMMQQ
FAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLGKKGLA
>Mature_454_residues
SHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVYQLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPL
FFMATGLFTFGTILAVLTQSFAIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGFM
ISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLAITSLENGSVNLYYLGLFILSFVL
FLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQLINLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKL
YDQKGARLSLYLGNSLFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADATAIFQMMQQF
AGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLGKKGLA

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=321, Percent_Identity=24.2990654205607, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081983, Length=424, Percent_Identity=24.0566037735849, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1789042, Length=435, Percent_Identity=21.1494252873563, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790195, Length=400, Percent_Identity=24.25, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788392, Length=431, Percent_Identity=23.4338747099768, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50395; Mature: 50264

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVYQLPLNSLQWMTTIYLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSFAIMLLARIFQGIGTGLVM
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
PQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGFMISHFSWQWIFICILPVPLI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHH
AGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLAITSLENGSVNLYYLGLFILSFV
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
LFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQLINLGINFLTPNFIVMEKIANSSQA
HHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHCCCCCC
GMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLGNSLFSLSLIIMTLQTRHFMLLPFTLL
CEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHH
YILFTFGRNMGFNNSLATAIRELPAEKNADATAIFQMMQQFAGALGTAMASLIANSQPEF
HHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
TSGVQSVYLLFTIFALLDFIFFFAMFYHLGKKGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVYQLPLNSLQWMTTIYLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSFAIMLLARIFQGIGTGLVM
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
PQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGFMISHFSWQWIFICILPVPLI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHH
AGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLAITSLENGSVNLYYLGLFILSFV
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
LFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQLINLGINFLTPNFIVMEKIANSSQA
HHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHCCCCCC
GMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLGNSLFSLSLIIMTLQTRHFMLLPFTLL
CEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHH
YILFTFGRNMGFNNSLATAIRELPAEKNADATAIFQMMQQFAGALGTAMASLIANSQPEF
HHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
TSGVQSVYLLFTIFALLDFIFFFAMFYHLGKKGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]