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The map label for this gene is exoA [H]

Identifier: 19745558

GI number: 19745558

Start: 373778

End: 374605

Strand: Reverse

Name: exoA [H]

Synonym: spyM18_0461

Alternate gene names: 19745558

Gene position: 374605-373778 (Counterclockwise)

Preceding gene: 19745566

Following gene: 19745556

Centisome position: 19.77

GC content: 43.36

Gene sequence:

>828_bases
ATGAAACTGATTTCATGGAACATTGATTCCTTAAATGCTGCTCTCACAGGAGAGTCCCCTCGTGCGCTTTTATCACGCGC
TGTTTTAGACACTTTAGTCGCTCAAGATGCGGATATTATTGCCATTCAAGAAACCAAATTATCTGCTAAGGGGCCTACCA
AAAAACATATCGAGGCCTTACTTAGTTACTTTCCAAACTATTTGCATGTGTGGCGTTCTTCAGTTGAGCCTGCTAGGAAA
GGCTATGCTGGCACCATGTTCCTGTACAAAAACACGCTAAACCCTGTCAGTACTTTTCCAGAAATTGGTGCCCCAACAAC
AATGGATGCTGAGGGCCGTATCATTACCCTAGAATTTGAAGACTTTTTCGTCACACAGGTTTACACGCCTAATGCTGGTG
ATGGCCTTAGACGTTTAGATGACCGTCAAATTTGGGACCACAAATACGCTGATTACTTGACTGAACTGGATGCTCAAAAG
CCTGTTTTGGCTGCGGGAGATTACAACGTTGCCCACAAAGAGATTGATTTGGCAAATCCAAGCAGCAATCGACGTTCCCC
TGGTTTTACCGACGAAGAACGTCAAAGCTTTACTAACCTATTAGCAAGAGGCTTTACAGATACTTTTCGTCACTTGCACG
GCGATATTCCTCATGTTTATACCTGGTGGGCACAACGTAGCAAAACCAGCAAAATCAATAATACCGGTTGGAGAATTGAC
TATTGGCTAGCTTCAAACCGACTTGTGGATAAGGTTAAGCGTTCTGAAATGATTTCTTCTGGGGAACGCCAAGACCATAC
GCCAATTTTACTAGATATTGATCTCTAA

Upstream 100 bases:

>100_bases
TTGGCTAGACTGTTTTATGGGGGCTAGGTGTCCAAAGTCGCTTATCGTTTCGTTTTTTGTTACAATAACAACAAGACTAT
CTGTTAGAAAGAGATACCAA

Downstream 100 bases:

>100_bases
TTTCTAAAAACAGATCCCAAAAAAGGACAAGGCGTTATAATGTTGCCTTGTCCTTTTTTGAGACTTTGTCGCATTATCCT
AAATCCAACTCTTGATATGG

Product: 3'-exo-deoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQETKLSAKGPTKKHIEALLSYFPNYLHVWRSSVEPARK
GYAGTMFLYKNTLNPVSTFPEIGAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
PVLAAGDYNVAHKEIDLANPSSNRRSPGFTDEERQSFTNLLARGFTDTFRHLHGDIPHVYTWWAQRSKTSKINNTGWRID
YWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL

Sequences:

>Translated_275_residues
MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQETKLSAKGPTKKHIEALLSYFPNYLHVWRSSVEPARK
GYAGTMFLYKNTLNPVSTFPEIGAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
PVLAAGDYNVAHKEIDLANPSSNRRSPGFTDEERQSFTNLLARGFTDTFRHLHGDIPHVYTWWAQRSKTSKINNTGWRID
YWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL
>Mature_275_residues
MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQETKLSAKGPTKKHIEALLSYFPNYLHVWRSSVEPARK
GYAGTMFLYKNTLNPVSTFPEIGAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
PVLAAGDYNVAHKEIDLANPSSNRRSPGFTDEERQSFTNLLARGFTDTFRHLHGDIPHVYTWWAQRSKTSKINNTGWRID
YWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL

Specific function: In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=276, Percent_Identity=37.3188405797101, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI18375503, Length=276, Percent_Identity=37.3188405797101, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI18375501, Length=276, Percent_Identity=37.3188405797101, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1788046, Length=287, Percent_Identity=28.2229965156794, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71989536, Length=275, Percent_Identity=37.0909090909091, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI71989539, Length=162, Percent_Identity=29.0123456790123, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221330655, Length=271, Percent_Identity=36.90036900369, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17136678, Length=271, Percent_Identity=36.90036900369, Blast_Score=168, Evalue=3e-42,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31256; Mature: 31256

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQETKLSAKGPTKKHIEAL
CCEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHH
LSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVSTFPEIGAPTTMDAEGRIITLEFE
HHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCCEEEEEEC
DFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQKPVLAAGDYNVAHKEIDLANP
CEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECCCC
SSNRRSPGFTDEERQSFTNLLARGFTDTFRHLHGDIPHVYTWWAQRSKTSKINNTGWRID
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEE
YWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL
EEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQETKLSAKGPTKKHIEAL
CCEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHH
LSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVSTFPEIGAPTTMDAEGRIITLEFE
HHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCCEEEEEEC
DFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQKPVLAAGDYNVAHKEIDLANP
CEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECCCC
SSNRRSPGFTDEERQSFTNLLARGFTDTFRHLHGDIPHVYTWWAQRSKTSKINNTGWRID
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEE
YWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL
EEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]