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The map label for this gene is speB [C]

Identifier: 18978329

GI number: 18978329

Start: 1807417

End: 1808382

Strand: Direct

Name: speB [C]

Synonym: PF1957

Alternate gene names: 18978329

Gene position: 1807417-1808382 (Clockwise)

Preceding gene: 18978328

Following gene: 18978330

Centisome position: 94.72

GC content: 42.75

Gene sequence:

>966_bases
ATGAAGATCCAGAGGAAGCTGCTAAGGCAGAAGGTCTCATTTGACTTTTCTTTTCTTTTGATAAAATTCTTAAACTCTTC
TCTAGCTCGTCTTTTTGGTGATGACATGCTGTTACATACCTACAAATCATTTGATATGGAACTGCCACTAGTCAACATAA
AAGATGCCGACTACGTGATTCTGGGCCTTCCCTATGATGGGACTACAAGCTATAAGCCAGGTGCAAGGTTTGGTCCAGTT
TTAATAAGGCAAGCTACTCTAAATTTTGAGAGCTACATTTTGGATTATGACGTTGATTTAGCCGAAGCTAAGATAGCTGA
CGCTGGAGATGTAGCACTACCTGTGAGTATAGAAGAGGCACTAAAGGTCGCTGAGGAAACTGTGAGAGAGTTGAGAGAAA
TAAATCCCAAAGCTTTGCCAATATTCCTAGGTGGAGAGCATTCTATGACGCTAGCCCCAGTAAGAGTCCTCAAACCTAAG
AGTTATGTAGTCTTTGATGCGCATTTGGACTTAAGAGACAGCTACGGGGGTTCAAAGTTCAACCATGCTTGTGTTGCTAG
AAGAATAAATGAGTTGAGAGTAGAAGTGGCAATATTTGGAGTGAGGAGTGGGACTAAGGAGGAGGTGGATTTTGCACGGA
AAAACGGAATAGAGTGGATTCATGCAAGAGACTACAGCTTTGAAGCGTTTGTAGATATTGTTAGCACTCTACCAGAGCCC
ATATACCTTTCAATTGACATAGATACCTTCGATGCATCTCTAGTTCCTGAAACTGGAACCCCAGAGCCTGGAGGATTAAG
ATTCTGGGACGTTGTGGAAGCTTTAGAATGGTTATCAGAGAGCAGGGAAGTCATTGGGATAGATATAATGGAAGTTGGAG
GAGAAAAGCTGGGCAATGTAACAGCCTTAACTGGGGCAAAGCTCCTCTTCTACCTCCTGGGAATGTTCGAGGGGATTAAG
AAATGA

Upstream 100 bases:

>100_bases
ATTGAAGCTGGAGGAGCTGGAGCAGTTGTTGGAAGAAATATATTCCAAAGAGAAAACCCAGAACCAATGATTAAGGCCCT
CATTAGGGTAATCCACAGAA

Downstream 100 bases:

>100_bases
AATTCCTCGCCACATGCCCCCCTGGGAGAGAAGGAGATGCAATGCTCGAGCTCGAGTGGGCGTTGAATGCCAAGGTGAAA
AGAACAAGGTGGGGGGGAGT

Product: agmatinase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKIQRKLLRQKVSFDFSFLLIKFLNSSLARLFGDDMLLHTYKSFDMELPLVNIKDADYVILGLPYDGTTSYKPGARFGPV
LIRQATLNFESYILDYDVDLAEAKIADAGDVALPVSIEEALKVAEETVRELREINPKALPIFLGGEHSMTLAPVRVLKPK
SYVVFDAHLDLRDSYGGSKFNHACVARRINELRVEVAIFGVRSGTKEEVDFARKNGIEWIHARDYSFEAFVDIVSTLPEP
IYLSIDIDTFDASLVPETGTPEPGGLRFWDVVEALEWLSESREVIGIDIMEVGGEKLGNVTALTGAKLLFYLLGMFEGIK
K

Sequences:

>Translated_321_residues
MKIQRKLLRQKVSFDFSFLLIKFLNSSLARLFGDDMLLHTYKSFDMELPLVNIKDADYVILGLPYDGTTSYKPGARFGPV
LIRQATLNFESYILDYDVDLAEAKIADAGDVALPVSIEEALKVAEETVRELREINPKALPIFLGGEHSMTLAPVRVLKPK
SYVVFDAHLDLRDSYGGSKFNHACVARRINELRVEVAIFGVRSGTKEEVDFARKNGIEWIHARDYSFEAFVDIVSTLPEP
IYLSIDIDTFDASLVPETGTPEPGGLRFWDVVEALEWLSESREVIGIDIMEVGGEKLGNVTALTGAKLLFYLLGMFEGIK
K
>Mature_321_residues
MKIQRKLLRQKVSFDFSFLLIKFLNSSLARLFGDDMLLHTYKSFDMELPLVNIKDADYVILGLPYDGTTSYKPGARFGPV
LIRQATLNFESYILDYDVDLAEAKIADAGDVALPVSIEEALKVAEETVRELREINPKALPIFLGGEHSMTLAPVRVLKPK
SYVVFDAHLDLRDSYGGSKFNHACVARRINELRVEVAIFGVRSGTKEEVDFARKNGIEWIHARDYSFEAFVDIVSTLPEP
IYLSIDIDTFDASLVPETGTPEPGGLRFWDVVEALEWLSESREVIGIDIMEVGGEKLGNVTALTGAKLLFYLLGMFEGIK
K

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=292, Percent_Identity=29.4520547945205, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1789306, Length=293, Percent_Identity=27.6450511945392, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 35963; Mature: 35963

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQRKLLRQKVSFDFSFLLIKFLNSSLARLFGDDMLLHTYKSFDMELPLVNIKDADYVI
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCCEEE
LGLPYDGTTSYKPGARFGPVLIRQATLNFESYILDYDVDLAEAKIADAGDVALPVSIEEA
EEECCCCCCCCCCCCCCCCEEEEEECCCHHHEEEECCCCHHHHCCCCCCCEEEEECHHHH
LKVAEETVRELREINPKALPIFLGGEHSMTLAPVRVLKPKSYVVFDAHLDLRDSYGGSKF
HHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCCEEEEEEEEEEECCCCCCCCH
NHACVARRINELRVEVAIFGVRSGTKEEVDFARKNGIEWIHARDYSFEAFVDIVSTLPEP
HHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCC
IYLSIDIDTFDASLVPETGTPEPGGLRFWDVVEALEWLSESREVIGIDIMEVGGEKLGNV
EEEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHCCE
TALTGAKLLFYLLGMFEGIKK
EHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIQRKLLRQKVSFDFSFLLIKFLNSSLARLFGDDMLLHTYKSFDMELPLVNIKDADYVI
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCCEEE
LGLPYDGTTSYKPGARFGPVLIRQATLNFESYILDYDVDLAEAKIADAGDVALPVSIEEA
EEECCCCCCCCCCCCCCCCEEEEEECCCHHHEEEECCCCHHHHCCCCCCCEEEEECHHHH
LKVAEETVRELREINPKALPIFLGGEHSMTLAPVRVLKPKSYVVFDAHLDLRDSYGGSKF
HHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCCEEEEEEEEEEECCCCCCCCH
NHACVARRINELRVEVAIFGVRSGTKEEVDFARKNGIEWIHARDYSFEAFVDIVSTLPEP
HHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCC
IYLSIDIDTFDASLVPETGTPEPGGLRFWDVVEALEWLSESREVIGIDIMEVGGEKLGNV
EEEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHCCE
TALTGAKLLFYLLGMFEGIKK
EHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]