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The map label for this gene is thiI [H]

Identifier: 18978207

GI number: 18978207

Start: 1691604

End: 1692209

Strand: Direct

Name: thiI [H]

Synonym: PF1835

Alternate gene names: 18978207

Gene position: 1691604-1692209 (Clockwise)

Preceding gene: 18978206

Following gene: 18978208

Centisome position: 88.65

GC content: 40.59

Gene sequence:

>606_bases
ATGAAGGTTGTAGCCCTCCTAAGCTCAGGCATAGACTCTCCCGTTGCAATTCATCTTATGGCTAGTAGGGGGGTAGAAGT
TTACCCTCTTCATTTTAGACAGGACGAGGTGAAGGAGAGAAAAGTTAGAAAAGTTGTCAAAAAGCTCCAAGAAATTCATG
GCGAAAAGGTTAAAGATCCACATACAGTGAACGCCTACGAAGTTCAAGCTCCTGTTTTCGAAAAGCTTAAGGAAATAAAG
AAAGGCAAGTGGACATGCGTGTTTTGTAAGTATACTATGTATATTGTTGCAGAGAGCTATGCAAAAAAAGTTGGAGCTAG
TGCAATAATTACGGGAGATTCACTAGGCCAGGTGGCTTCCCAAACTCTTGACAATCTCCTAGTCATAAGCACAGCTACAA
AATTGCCAGTAATAAGACCACTAATTGGGCTTGATAAAGAGGAGATAGTTAAAATAGCAAAGGAAATTGGGACGTTTGAA
ATTAGCATAGAAGAAGAACCTCCTTGTCCATTCGTTCCGAAGTTCCCAGTGGTTAGAGCTGGATTAGGAGAATTTCAGAA
GATTTTATCTCAAGTGAAAGACAAACTTCCCAGGGGAGATTTATGA

Upstream 100 bases:

>100_bases
CAATTCCAGAGCTAATAGGAGGTACAGTAATTCTTGGATACATGATATATCTGGGCTTGCGAGAAGAAAAAATATATACC
AAAAACTCAACCTCTAACCC

Downstream 100 bases:

>100_bases
AACCCTTAAAGCTTGCAACTTATCTTCTAATCTTAAATTCCTTTCTTCTGCTTTTATATAGTTATTCAATCTACTATGCA
TTCGCAATATTTAGCTTTGT

Product: hypothetical protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MKVVALLSSGIDSPVAIHLMASRGVEVYPLHFRQDEVKERKVRKVVKKLQEIHGEKVKDPHTVNAYEVQAPVFEKLKEIK
KGKWTCVFCKYTMYIVAESYAKKVGASAIITGDSLGQVASQTLDNLLVISTATKLPVIRPLIGLDKEEIVKIAKEIGTFE
ISIEEEPPCPFVPKFPVVRAGLGEFQKILSQVKDKLPRGDL

Sequences:

>Translated_201_residues
MKVVALLSSGIDSPVAIHLMASRGVEVYPLHFRQDEVKERKVRKVVKKLQEIHGEKVKDPHTVNAYEVQAPVFEKLKEIK
KGKWTCVFCKYTMYIVAESYAKKVGASAIITGDSLGQVASQTLDNLLVISTATKLPVIRPLIGLDKEEIVKIAKEIGTFE
ISIEEEPPCPFVPKFPVVRAGLGEFQKILSQVKDKLPRGDL
>Mature_201_residues
MKVVALLSSGIDSPVAIHLMASRGVEVYPLHFRQDEVKERKVRKVVKKLQEIHGEKVKDPHTVNAYEVQAPVFEKLKEIK
KGKWTCVFCKYTMYIVAESYAKKVGASAIITGDSLGQVASQTLDNLLVISTATKLPVIRPLIGLDKEEIVKIAKEIGTFE
ISIEEEPPCPFVPKFPVVRAGLGEFQKILSQVKDKLPRGDL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=180, Percent_Identity=32.7777777777778, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 22367; Mature: 22367

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVALLSSGIDSPVAIHLMASRGVEVYPLHFRQDEVKERKVRKVVKKLQEIHGEKVKDP
CHHHEEHHCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
HTVNAYEVQAPVFEKLKEIKKGKWTCVFCKYTMYIVAESYAKKVGASAIITGDSLGQVAS
CCCCCEEECCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHH
QTLDNLLVISTATKLPVIRPLIGLDKEEIVKIAKEIGTFEISIEEEPPCPFVPKFPVVRA
HHHHCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHH
GLGEFQKILSQVKDKLPRGDL
CHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKVVALLSSGIDSPVAIHLMASRGVEVYPLHFRQDEVKERKVRKVVKKLQEIHGEKVKDP
CHHHEEHHCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
HTVNAYEVQAPVFEKLKEIKKGKWTCVFCKYTMYIVAESYAKKVGASAIITGDSLGQVAS
CCCCCEEECCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHH
QTLDNLLVISTATKLPVIRPLIGLDKEEIVKIAKEIGTFEISIEEEPPCPFVPKFPVVRA
HHHHCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHH
GLGEFQKILSQVKDKLPRGDL
CHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA