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The map label for this gene is truB

Identifier: 18978157

GI number: 18978157

Start: 1660205

End: 1661227

Strand: Direct

Name: truB

Synonym: PF1785

Alternate gene names: 18978157

Gene position: 1660205-1661227 (Clockwise)

Preceding gene: 18978153

Following gene: 18978161

Centisome position: 87.0

GC content: 41.06

Gene sequence:

>1023_bases
GTGGCGAGAGACGAGGTAAGAAGGATACTTCCAGCAGATATAAAAAGAGAAGTTCTAATTAAAGATGAAAACGCGGAAAC
TAATCCTGATTGGGGATTTCCTCCAGAAAAAAGGCCAATAGAAATGCACATTCAATTTGGAGTCATAAACTTAGACAAAC
CTCCAGGACCAACAAGTCATGAAGTAGTTGCCTGGATTAAGAAGATACTAAATCTCGAAAAAGCAGGACACGGAGGAACT
TTAGATCCCAAAGTTAGTGGCGTGCTTCCTGTGGCACTTGAAAAAGCCACAAGAGTAGTCCAGGCCCTACTTCCTGCAGG
AAAGGAATACGTAGCATTAATGCATCTTCATGGGGATGTACCAGAGGACAAGATAATTCAGGTAATGAAAGAGTTTGAGG
GAGAAATAATTCAGAGACCCCCACTTAGAAGCGCTGTGAAAAGAAGACTTAGAACTAGAAAAGTTTACTATATTGAGGTT
CTTGAAATAGAGGGAAGAGATGTTCTCTTTAGAGTAGGAGTTGAGGCAGGAACGTACATAAGATCACTTATTCACCACAT
TGGCCTGGCCCTTGGAGTTGGCGCCCATATGTCTGAGCTGAGAAGAACAAGAAGCGGACCATTCAAAGAAGATGAAACTT
TAATAACCCTTCACGATCTTGTGGACTATTATTACTTCTGGAAAGAAGATGGAATTGAAGAGTATTTTAGAAAAGCAATA
CAGCCGATGGAAAAGGCGGTTGAACACCTACCAAAGGTCTGGATAAAAGACTCAGCAGTTGCAGCTGTAACTCACGGAGC
TGATCTGGCAGTTCCTGGGATAGCTAAGCTTCATGCAGGGATAAAAAGAGGTGATTTAGTGGCAATTATGACATTAAAAG
ATGAGTTAGTAGCTTTAGGAAAGGCCATGATGACGAGTCAAGAGATGTTAGAAAAAACAAAAGGAATAGCCGTTGATGTA
GAAAAGGTATTCATGCCTAGGGATTGGTATCCAAAGTTATGGGAAAAAAGAGATAGAAGCTAA

Upstream 100 bases:

>100_bases
CAGTGTTATTTGACCAGGGATATAATAATGAGATTATTTAAAAAACTTTTTCAATTTAGATGCCTAGGGAGATAAAAGAG
ATCCAAAGTAGGTGGTGGAA

Downstream 100 bases:

>100_bases
ATTCATGATACTCTTGTTTTTTCTTTTCTCAATGAATATGTGAGTCCATAAGCTGGCCATAACCCAGGGGGCTGAGAATA
GTGATGGAGGTTCCTTAAAA

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MARDEVRRILPADIKREVLIKDENAETNPDWGFPPEKRPIEMHIQFGVINLDKPPGPTSHEVVAWIKKILNLEKAGHGGT
LDPKVSGVLPVALEKATRVVQALLPAGKEYVALMHLHGDVPEDKIIQVMKEFEGEIIQRPPLRSAVKRRLRTRKVYYIEV
LEIEGRDVLFRVGVEAGTYIRSLIHHIGLALGVGAHMSELRRTRSGPFKEDETLITLHDLVDYYYFWKEDGIEEYFRKAI
QPMEKAVEHLPKVWIKDSAVAAVTHGADLAVPGIAKLHAGIKRGDLVAIMTLKDELVALGKAMMTSQEMLEKTKGIAVDV
EKVFMPRDWYPKLWEKRDRS

Sequences:

>Translated_340_residues
MARDEVRRILPADIKREVLIKDENAETNPDWGFPPEKRPIEMHIQFGVINLDKPPGPTSHEVVAWIKKILNLEKAGHGGT
LDPKVSGVLPVALEKATRVVQALLPAGKEYVALMHLHGDVPEDKIIQVMKEFEGEIIQRPPLRSAVKRRLRTRKVYYIEV
LEIEGRDVLFRVGVEAGTYIRSLIHHIGLALGVGAHMSELRRTRSGPFKEDETLITLHDLVDYYYFWKEDGIEEYFRKAI
QPMEKAVEHLPKVWIKDSAVAAVTHGADLAVPGIAKLHAGIKRGDLVAIMTLKDELVALGKAMMTSQEMLEKTKGIAVDV
EKVFMPRDWYPKLWEKRDRS
>Mature_339_residues
ARDEVRRILPADIKREVLIKDENAETNPDWGFPPEKRPIEMHIQFGVINLDKPPGPTSHEVVAWIKKILNLEKAGHGGTL
DPKVSGVLPVALEKATRVVQALLPAGKEYVALMHLHGDVPEDKIIQVMKEFEGEIIQRPPLRSAVKRRLRTRKVYYIEVL
EIEGRDVLFRVGVEAGTYIRSLIHHIGLALGVGAHMSELRRTRSGPFKEDETLITLHDLVDYYYFWKEDGIEEYFRKAIQ
PMEKAVEHLPKVWIKDSAVAAVTHGADLAVPGIAKLHAGIKRGDLVAIMTLKDELVALGKAMMTSQEMLEKTKGIAVDVE
KVFMPRDWYPKLWEKRDRS

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI215599015, Length=306, Percent_Identity=42.8104575163399, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI4503337, Length=306, Percent_Identity=42.8104575163399, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI21040257, Length=218, Percent_Identity=32.5688073394495, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI2367200, Length=263, Percent_Identity=28.1368821292776, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17553978, Length=306, Percent_Identity=40.8496732026144, Blast_Score=257, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6323204, Length=306, Percent_Identity=43.7908496732026, Blast_Score=275, Evalue=5e-75,
Organism=Drosophila melanogaster, GI281364189, Length=306, Percent_Identity=44.4444444444444, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI281364187, Length=306, Percent_Identity=44.4444444444444, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI281364185, Length=306, Percent_Identity=44.4444444444444, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI281364183, Length=306, Percent_Identity=44.4444444444444, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI62471759, Length=306, Percent_Identity=44.4444444444444, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI17975520, Length=306, Percent_Identity=44.4444444444444, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PYRFU (Q7LWY0)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579514.1
- PDB:   2EY4
- PDB:   2HVY
- PDB:   2RFK
- PDB:   3HAX
- PDB:   3HAY
- PDB:   3HJW
- PDB:   3HJY
- PDB:   3LWO
- PDB:   3LWP
- PDB:   3LWQ
- PDB:   3LWR
- PDB:   3LWV
- PDBsum:   2EY4
- PDBsum:   2HVY
- PDBsum:   2RFK
- PDBsum:   3HAX
- PDBsum:   3HAY
- PDBsum:   3HJW
- PDBsum:   3HJY
- PDBsum:   3LWO
- PDBsum:   3LWP
- PDBsum:   3LWQ
- PDBsum:   3LWR
- PDBsum:   3LWV
- ProteinModelPortal:   Q7LWY0
- SMR:   Q7LWY0
- DIP:   DIP-48526N
- EnsemblBacteria:   EBPYRT00000004105
- GeneID:   1469664
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1785
- GeneTree:   EBGT00050000022953
- HOGENOM:   HBG397258
- OMA:   SGPTSHE
- ProtClustDB:   PRK04270
- HAMAP:   MF_01081
- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR004521
- SMART:   SM00359
- TIGRFAMs:   TIGR00425
- TIGRFAMs:   TIGR00451

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 38562; Mature: 38430

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 82-82

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDEVRRILPADIKREVLIKDENAETNPDWGFPPEKRPIEMHIQFGVINLDKPPGPTSH
CCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCHH
EVVAWIKKILNLEKAGHGGTLDPKVSGVLPVALEKATRVVQALLPAGKEYVALMHLHGDV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCC
PEDKIIQVMKEFEGEIIQRPPLRSAVKRRLRTRKVYYIEVLEIEGRDVLFRVGVEAGTYI
CHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCEEEEEEEEEECCCEEEEEECCCHHHHH
RSLIHHIGLALGVGAHMSELRRTRSGPFKEDETLITLHDLVDYYYFWKEDGIEEYFRKAI
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHH
QPMEKAVEHLPKVWIKDSAVAAVTHGADLAVPGIAKLHAGIKRGDLVAIMTLKDELVALG
HHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHH
KAMMTSQEMLEKTKGIAVDVEKVFMPRDWYPKLWEKRDRS
HHHHHHHHHHHHHCCCEEEHHHHHCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
ARDEVRRILPADIKREVLIKDENAETNPDWGFPPEKRPIEMHIQFGVINLDKPPGPTSH
CHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCHH
EVVAWIKKILNLEKAGHGGTLDPKVSGVLPVALEKATRVVQALLPAGKEYVALMHLHGDV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCC
PEDKIIQVMKEFEGEIIQRPPLRSAVKRRLRTRKVYYIEVLEIEGRDVLFRVGVEAGTYI
CHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCEEEEEEEEEECCCEEEEEECCCHHHHH
RSLIHHIGLALGVGAHMSELRRTRSGPFKEDETLITLHDLVDYYYFWKEDGIEEYFRKAI
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHH
QPMEKAVEHLPKVWIKDSAVAAVTHGADLAVPGIAKLHAGIKRGDLVAIMTLKDELVALG
HHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHH
KAMMTSQEMLEKTKGIAVDVEKVFMPRDWYPKLWEKRDRS
HHHHHHHHHHHHHCCCEEEHHHHHCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA