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The map label for this gene is rnhB

Identifier: 18978153

GI number: 18978153

Start: 1655335

End: 1656009

Strand: Direct

Name: rnhB

Synonym: PF1781

Alternate gene names: 18978153

Gene position: 1655335-1656009 (Clockwise)

Preceding gene: 18978152

Following gene: 18978157

Centisome position: 86.75

GC content: 39.41

Gene sequence:

>675_bases
ATGAAAATAGGGGGAATTGACGAAGCAGGAAGAGGACCAGCGATAGGGCCATTAGTAGTAGCTACTGTCGTCGTTGATGA
GAAAAACATTGAGAAGCTCAGAAACATTGGAGTAAAAGACTCCAAACAACTAACACCCCATGAAAGGAAGAATTTATTTT
CCCAGATAACCTCAATAGCGGATGATTACAAAATAGTGATAGTATCCCCAGAAGAAATCGACAATAGATCAGGAACAATG
AACGAGTTAGAGGTAGAGAAGTTTGCTCTCGCCCTAAATTCGCTTCAGATAAAACCAGCTCTTATATACGCTGATGCAGC
GGATGTAGATGCCAATAGATTTGCAAGCTTGATAGAGAGAAGACTCAATTATAAGGCGAAGATTATTGCCGAACACAAGG
CCGATGCAAAGTATCCAGTAGTTTCAGCAGCTTCAATACTTGCAAAGGTTGTTAGGGATGAGGAAATTGAAAAATTAAAA
AAGCAATATGGAGACTTTGGCTCTGGGTATCCAAGTGATCCAAAAACCAAGAAATGGCTTGAAGAGTACTACAAAAAACA
CAACTCTTTCCCTCCAATAGTCAGACGAACCTGGGAAACTGTAAGAAAAATAGAGGAAAGCATTAAAGCCAAAAAATCCC
AGCTAACGCTTGATAAATTCTTTAAGAAACCTTAG

Upstream 100 bases:

>100_bases
CGGTAATTTTTGGCTATTACTCTAACATTCCTCCCAGTGGAGTTGCCACAATAATCTTGGGTATAATCTATGGAGGAATA
ATATCCAGGAGGAGAGAATA

Downstream 100 bases:

>100_bases
AAAGTCTATCCACAACCTTCCATATTCTTTGATCCACTCCCAGTAGATCTTCAAAGATTTTTCGAAGTACTCCCACTTTA
TTATTTCATAGCTAATGACT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKIGGIDEAGRGPAIGPLVVATVVVDEKNIEKLRNIGVKDSKQLTPHERKNLFSQITSIADDYKIVIVSPEEIDNRSGTM
NELEVEKFALALNSLQIKPALIYADAADVDANRFASLIERRLNYKAKIIAEHKADAKYPVVSAASILAKVVRDEEIEKLK
KQYGDFGSGYPSDPKTKKWLEEYYKKHNSFPPIVRRTWETVRKIEESIKAKKSQLTLDKFFKKP

Sequences:

>Translated_224_residues
MKIGGIDEAGRGPAIGPLVVATVVVDEKNIEKLRNIGVKDSKQLTPHERKNLFSQITSIADDYKIVIVSPEEIDNRSGTM
NELEVEKFALALNSLQIKPALIYADAADVDANRFASLIERRLNYKAKIIAEHKADAKYPVVSAASILAKVVRDEEIEKLK
KQYGDFGSGYPSDPKTKKWLEEYYKKHNSFPPIVRRTWETVRKIEESIKAKKSQLTLDKFFKKP
>Mature_224_residues
MKIGGIDEAGRGPAIGPLVVATVVVDEKNIEKLRNIGVKDSKQLTPHERKNLFSQITSIADDYKIVIVSPEEIDNRSGTM
NELEVEKFALALNSLQIKPALIYADAADVDANRFASLIERRLNYKAKIIAEHKADAKYPVVSAASILAKVVRDEEIEKLK
KQYGDFGSGYPSDPKTKKWLEEYYKKHNSFPPIVRRTWETVRKIEESIKAKKSQLTLDKFFKKP

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Homo sapiens, GI38455391, Length=222, Percent_Identity=36.4864864864865, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1786380, Length=202, Percent_Identity=32.6732673267327, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17536263, Length=227, Percent_Identity=34.3612334801762, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324257, Length=223, Percent_Identity=33.1838565022422, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24580635, Length=251, Percent_Identity=33.8645418326693, Blast_Score=111, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_PYRFU (Q8U036)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579510.1
- ProteinModelPortal:   Q8U036
- SMR:   Q8U036
- EnsemblBacteria:   EBPYRT00000005767
- GeneID:   1469660
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1781
- GeneTree:   EBGT00050000022530
- HOGENOM:   HBG584843
- OMA:   AEHKADE
- ProtClustDB:   PRK00015
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_A
- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337
- Gene3D:   G3DSA:1.10.10.460
- PANTHER:   PTHR10954
- TIGRFAMs:   TIGR00729

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 25315; Mature: 25315

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGGIDEAGRGPAIGPLVVATVVVDEKNIEKLRNIGVKDSKQLTPHERKNLFSQITSIA
CCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
DDYKIVIVSPEEIDNRSGTMNELEVEKFALALNSLQIKPALIYADAADVDANRFASLIER
CCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
RLNYKAKIIAEHKADAKYPVVSAASILAKVVRDEEIEKLKKQYGDFGSGYPSDPKTKKWL
HHCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
EEYYKKHNSFPPIVRRTWETVRKIEESIKAKKSQLTLDKFFKKP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIGGIDEAGRGPAIGPLVVATVVVDEKNIEKLRNIGVKDSKQLTPHERKNLFSQITSIA
CCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
DDYKIVIVSPEEIDNRSGTMNELEVEKFALALNSLQIKPALIYADAADVDANRFASLIER
CCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
RLNYKAKIIAEHKADAKYPVVSAASILAKVVRDEEIEKLKKQYGDFGSGYPSDPKTKKWL
HHCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
EEYYKKHNSFPPIVRRTWETVRKIEESIKAKKSQLTLDKFFKKP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA