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The map label for this gene is glyA [H]

Identifier: 18978150

GI number: 18978150

Start: 1652268

End: 1653551

Strand: Reverse

Name: glyA [H]

Synonym: PF1778

Alternate gene names: 18978150

Gene position: 1653551-1652268 (Counterclockwise)

Preceding gene: 18978154

Following gene: 18978149

Centisome position: 86.65

GC content: 44.16

Gene sequence:

>1284_bases
ATGACATATAAGGAGTATAGAGACAAGGTTCTAAACTTTATAGAGGAGCATGAAAAATGGAGAAGCCACACTATAAACCT
CATTGCAAGTGAAAACATAACATCCCCGAGTGTAAACAGAGCCGTTGCTTCAGGCTTCATGCACAAGTATGCTGAGGGCT
GGCCGAAGCAGAGGTACTATCAGGGATGTAAATACGTTGATGAGGTTGAGCTCATTGGAGTAGAGCTATTCACAAAGTTA
TTTAAGAGTGATTACGCTGATTTAAGACCAATTTCTGGAACTAATGCAAATCAGGCGGTATTCTTTGGTCTTGGACAACC
AGGTGACAAGGTTATAGTTCTTCACACTAGCCACGGAGGTCACATAAGCCACATGCCCTTCGGAGCTGCTGGAATGAGAG
GACTAGAAGTACACACTTGGCCATTTGACTTTGAGAGCTTTAACATCGATGTTGATAAGGCAGAGAAAATGATAAGGGAG
TTAGAACCAAAGATAGTGATGTTTGGTGGCTCACTATTCCCATTTCCACACCCAGTAAAGGAACTAGCTCCAGTGGCTAA
GGAGGTTGGTGCCTTTGTTGTCTATGATGCCGCTCACGTACTAGGTCTCATAGCTGGAGGAGAGTTCCAGGATCCACTTA
GGGAAGGAGCAGACATAATGACAGCTTCAACACACAAGACATTCCCAGGTCCTCAGGGTGGAGTTATACTCTACAAAAAG
TTTGCAGACGATGAAACAATAGCAAAGTTACAATGGGCAATATTCCCAGGAGTAGTGAGCAATCACCACCTTCACCACAT
GGCTGGTAAGGTTATTACAGCAGCAGAAATGTTGGAGTATGGTGAAGCGTATGCAAAGCAGATAGTTAAGAATGCTAAGG
CTCTTGCTGAAGCTTTGGCTGAAGAGGGGTTCAAGGTTATCGGTGAAGATCAAGGCTATACAAAGAGCCACCAAGTTATA
GTGGACGTAAGTGACCTACACCCAGCCGGTGGAGGATGGGCTGCTCCACTTCTTGAAGAAGCTGGAATAATCCTCAACAA
GAACCTACTTCCATGGGATCCACTTGAAAAAGTCAACGAGCCCAGTGGATTAAGAATTGGAGTTCAAGAGATGACAAGAG
TTGGAATGATGGAAGATGAAATGAGAGAAATCGCCCACTTCATCAAGAGAGTTCTAATAGATAAGGAAGATCCAAAGAAG
GTCAGAAAGGATGTCTACTACTTCAGGCTAGAGTACCAGAAGGTTTACTACTCCTTCGACTATGGCCTACCAATGAAGGA
GTGA

Upstream 100 bases:

>100_bases
AAAGATTGTGAAAAAGATAGTAAACTCAACAATTATCGAATTTCCTGGGCAAACCTTTAAATTATATGATCATGAAATAG
TGTATCGAGGTGAATTTTCG

Downstream 100 bases:

>100_bases
AGTTTTTAATTTCTTTTTTCTCACTTATTTTTGTGAGTTGTCATGAGGAGAATCAAGGTGATATTTTTTGATTTGGATGA
TACTTTGGTTGATACTAGCA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTYKEYRDKVLNFIEEHEKWRSHTINLIASENITSPSVNRAVASGFMHKYAEGWPKQRYYQGCKYVDEVELIGVELFTKL
FKSDYADLRPISGTNANQAVFFGLGQPGDKVIVLHTSHGGHISHMPFGAAGMRGLEVHTWPFDFESFNIDVDKAEKMIRE
LEPKIVMFGGSLFPFPHPVKELAPVAKEVGAFVVYDAAHVLGLIAGGEFQDPLREGADIMTASTHKTFPGPQGGVILYKK
FADDETIAKLQWAIFPGVVSNHHLHHMAGKVITAAEMLEYGEAYAKQIVKNAKALAEALAEEGFKVIGEDQGYTKSHQVI
VDVSDLHPAGGGWAAPLLEEAGIILNKNLLPWDPLEKVNEPSGLRIGVQEMTRVGMMEDEMREIAHFIKRVLIDKEDPKK
VRKDVYYFRLEYQKVYYSFDYGLPMKE

Sequences:

>Translated_427_residues
MTYKEYRDKVLNFIEEHEKWRSHTINLIASENITSPSVNRAVASGFMHKYAEGWPKQRYYQGCKYVDEVELIGVELFTKL
FKSDYADLRPISGTNANQAVFFGLGQPGDKVIVLHTSHGGHISHMPFGAAGMRGLEVHTWPFDFESFNIDVDKAEKMIRE
LEPKIVMFGGSLFPFPHPVKELAPVAKEVGAFVVYDAAHVLGLIAGGEFQDPLREGADIMTASTHKTFPGPQGGVILYKK
FADDETIAKLQWAIFPGVVSNHHLHHMAGKVITAAEMLEYGEAYAKQIVKNAKALAEALAEEGFKVIGEDQGYTKSHQVI
VDVSDLHPAGGGWAAPLLEEAGIILNKNLLPWDPLEKVNEPSGLRIGVQEMTRVGMMEDEMREIAHFIKRVLIDKEDPKK
VRKDVYYFRLEYQKVYYSFDYGLPMKE
>Mature_426_residues
TYKEYRDKVLNFIEEHEKWRSHTINLIASENITSPSVNRAVASGFMHKYAEGWPKQRYYQGCKYVDEVELIGVELFTKLF
KSDYADLRPISGTNANQAVFFGLGQPGDKVIVLHTSHGGHISHMPFGAAGMRGLEVHTWPFDFESFNIDVDKAEKMIREL
EPKIVMFGGSLFPFPHPVKELAPVAKEVGAFVVYDAAHVLGLIAGGEFQDPLREGADIMTASTHKTFPGPQGGVILYKKF
ADDETIAKLQWAIFPGVVSNHHLHHMAGKVITAAEMLEYGEAYAKQIVKNAKALAEALAEEGFKVIGEDQGYTKSHQVIV
DVSDLHPAGGGWAAPLLEEAGIILNKNLLPWDPLEKVNEPSGLRIGVQEMTRVGMMEDEMREIAHFIKRVLIDKEDPKKV
RKDVYYFRLEYQKVYYSFDYGLPMKE

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI22547186, Length=404, Percent_Identity=32.1782178217822, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI261862346, Length=394, Percent_Identity=30.4568527918782, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI19923315, Length=399, Percent_Identity=30.8270676691729, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI261862352, Length=399, Percent_Identity=30.8270676691729, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI261862350, Length=399, Percent_Identity=30.8270676691729, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI261862348, Length=399, Percent_Identity=30.8270676691729, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI22547189, Length=391, Percent_Identity=31.2020460358056, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1788902, Length=405, Percent_Identity=36.0493827160494, Blast_Score=219, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI25144729, Length=402, Percent_Identity=30.5970149253731, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI25144732, Length=402, Percent_Identity=30.5970149253731, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323087, Length=410, Percent_Identity=30.9756097560976, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6319739, Length=418, Percent_Identity=27.511961722488, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24640005, Length=403, Percent_Identity=32.7543424317618, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI221329721, Length=403, Percent_Identity=32.7543424317618, Blast_Score=201, Evalue=6e-52,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 47988; Mature: 47856

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYKEYRDKVLNFIEEHEKWRSHTINLIASENITSPSVNRAVASGFMHKYAEGWPKQRYY
CCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
QGCKYVDEVELIGVELFTKLFKSDYADLRPISGTNANQAVFFGLGQPGDKVIVLHTSHGG
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCC
HISHMPFGAAGMRGLEVHTWPFDFESFNIDVDKAEKMIRELEPKIVMFGGSLFPFPHPVK
EECCCCCCCCCCCCCEEEECCCCCCCEECCHHHHHHHHHHCCCCEEEECCCCCCCCCCHH
ELAPVAKEVGAFVVYDAAHVLGLIAGGEFQDPLREGADIMTASTHKTFPGPQGGVILYKK
HHHHHHHHHCCEEEEHHHHHHHHHCCCCCHHHHHCCCCEEEECCCCCCCCCCCCEEEEEE
FADDETIAKLQWAIFPGVVSNHHLHHMAGKVITAAEMLEYGEAYAKQIVKNAKALAEALA
CCCCHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGFKVIGEDQGYTKSHQVIVDVSDLHPAGGGWAAPLLEEAGIILNKNLLPWDPLEKVNE
HCCCEEECCCCCCCCCCEEEEEEHHCCCCCCCHHHHHHHHCCEEEECCCCCCCCHHHCCC
PSGLRIGVQEMTRVGMMEDEMREIAHFIKRVLIDKEDPKKVRKDVYYFRLEYQKVYYSFD
CCCCEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEEEEEEEEEEE
YGLPMKE
CCCCCCC
>Mature Secondary Structure 
TYKEYRDKVLNFIEEHEKWRSHTINLIASENITSPSVNRAVASGFMHKYAEGWPKQRYY
CHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
QGCKYVDEVELIGVELFTKLFKSDYADLRPISGTNANQAVFFGLGQPGDKVIVLHTSHGG
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCC
HISHMPFGAAGMRGLEVHTWPFDFESFNIDVDKAEKMIRELEPKIVMFGGSLFPFPHPVK
EECCCCCCCCCCCCCEEEECCCCCCCEECCHHHHHHHHHHCCCCEEEECCCCCCCCCCHH
ELAPVAKEVGAFVVYDAAHVLGLIAGGEFQDPLREGADIMTASTHKTFPGPQGGVILYKK
HHHHHHHHHCCEEEEHHHHHHHHHCCCCCHHHHHCCCCEEEECCCCCCCCCCCCEEEEEE
FADDETIAKLQWAIFPGVVSNHHLHHMAGKVITAAEMLEYGEAYAKQIVKNAKALAEALA
CCCCHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGFKVIGEDQGYTKSHQVIVDVSDLHPAGGGWAAPLLEEAGIILNKNLLPWDPLEKVNE
HCCCEEECCCCCCCCCCEEEEEEHHCCCCCCCHHHHHHHHCCEEEECCCCCCCCHHHCCC
PSGLRIGVQEMTRVGMMEDEMREIAHFIKRVLIDKEDPKKVRKDVYYFRLEYQKVYYSFD
CCCCEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEEEEEEEEEEE
YGLPMKE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]