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The map label for this gene is carA

Identifier: 18978085

GI number: 18978085

Start: 1590675

End: 1591790

Strand: Direct

Name: carA

Synonym: PF1713

Alternate gene names: 18978085

Gene position: 1590675-1591790 (Clockwise)

Preceding gene: 18978084

Following gene: 18978086

Centisome position: 83.36

GC content: 39.34

Gene sequence:

>1116_bases
ATGGGGGTGCACAAAAAAGGGTACTTAGTTTTGGAAGATGGCTCAGTGATAGAAGGGAAGGCCTTTGGTGCAGAAACGAT
TCGATATGGGGAAGTAGTCTTCACAACGGCAATGGTGGGATATCCAGAATCCCTAACTGATCCTTCATATAAAGGCCAAA
TCTTAATTGCAACAAATCCCTTAATGGGAACTTATGGAGTTTCATCAAAAAGTCTAAAAGAACATGGACTGCCTCTACAT
TACGAATCGGACTCAATTAAGGTAGAAGGATTTGTTATATCTTTTCTAATGAGGCCTAATCACTGGAGCAGCGAAATGAC
TCTAGATGAATGGTTAAGGAGGGAGGGCGTTCCAGGGCTCTATGGAGTTGATACTAGAGCCTTAGTCAAGAAGATTAGAG
AGGAGGGTGTAATGAGAGGAGCAATAGTCACAACAGAAACAGATTTAGAAGAAATTTTAGAGAATATTAGAAAAATAAGC
TATGAAAGTACCAATTTCGTTGAACTTGTATCGCCAAAAAACCCAATTGTACACACACCAAAAAATGTCAAATTCAGAGT
TGTGGTTCTTGATTTGGGAGTGAAGTTTGGTATTCTTAGAGAGTTACTCAAGAGGGGAATTGAAGTTGTTAGAATTCCAT
GGAATTGGGACATATTGGAGGCCTATTACTCCTACAACGCAGATGGCATATTCATAAGCAATGGGCCTGGAAATCCTACC
CTCTTAAAAGAAGTTGCTAAGAGGATTAGAAAGGCATTCAATGAAGAAATTCCGATGATGGGAATATGCCTTGGCCAACA
ACTTTTAGCCTTAGCAGATGGGGCAGAAATATACAAGCTTAAATACGGTCATAGAGGGATTAACAAACCTGTTAAAGATT
TAGAGAGTGGAAAAGCATTTGTTACTACTCAAAATCATGGCTATGCAATTGTTCCTGAAAGCCTAAATGAGTTTAAGGTA
TGGATGGTAAACCTAGATGATAACTCTGTAGAGGGAATAAAACACGAAAGTTTACCTATTATAGCTACCCAATATCATCC
CGAGGCTTCTCCTGGCCCCTGGGATTCATTGTGGGTTTTTGATGAATTTGTAAAGATTTTGGAGGGGAGAAAATGA

Upstream 100 bases:

>100_bases
CTATAAATTTTGTTCTATTTGCAACTTTCTATTATATTTTTGCAGTTGATGTTACAAAAGAAATAAAAATCAGAAAAGAC
TGAGGGCAAGAGGGCATTTA

Downstream 100 bases:

>100_bases
AAATCGATGTATCAAAAGTCATTGTTATTGGTTCCGGTGCAATAAAAATAGGTGAAGCAGCTGAGTTTGACTACAGTGGA
AGTCAAGCATTAAAAGCATT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MGVHKKGYLVLEDGSVIEGKAFGAETIRYGEVVFTTAMVGYPESLTDPSYKGQILIATNPLMGTYGVSSKSLKEHGLPLH
YESDSIKVEGFVISFLMRPNHWSSEMTLDEWLRREGVPGLYGVDTRALVKKIREEGVMRGAIVTTETDLEEILENIRKIS
YESTNFVELVSPKNPIVHTPKNVKFRVVVLDLGVKFGILRELLKRGIEVVRIPWNWDILEAYYSYNADGIFISNGPGNPT
LLKEVAKRIRKAFNEEIPMMGICLGQQLLALADGAEIYKLKYGHRGINKPVKDLESGKAFVTTQNHGYAIVPESLNEFKV
WMVNLDDNSVEGIKHESLPIIATQYHPEASPGPWDSLWVFDEFVKILEGRK

Sequences:

>Translated_371_residues
MGVHKKGYLVLEDGSVIEGKAFGAETIRYGEVVFTTAMVGYPESLTDPSYKGQILIATNPLMGTYGVSSKSLKEHGLPLH
YESDSIKVEGFVISFLMRPNHWSSEMTLDEWLRREGVPGLYGVDTRALVKKIREEGVMRGAIVTTETDLEEILENIRKIS
YESTNFVELVSPKNPIVHTPKNVKFRVVVLDLGVKFGILRELLKRGIEVVRIPWNWDILEAYYSYNADGIFISNGPGNPT
LLKEVAKRIRKAFNEEIPMMGICLGQQLLALADGAEIYKLKYGHRGINKPVKDLESGKAFVTTQNHGYAIVPESLNEFKV
WMVNLDDNSVEGIKHESLPIIATQYHPEASPGPWDSLWVFDEFVKILEGRK
>Mature_370_residues
GVHKKGYLVLEDGSVIEGKAFGAETIRYGEVVFTTAMVGYPESLTDPSYKGQILIATNPLMGTYGVSSKSLKEHGLPLHY
ESDSIKVEGFVISFLMRPNHWSSEMTLDEWLRREGVPGLYGVDTRALVKKIREEGVMRGAIVTTETDLEEILENIRKISY
ESTNFVELVSPKNPIVHTPKNVKFRVVVLDLGVKFGILRELLKRGIEVVRIPWNWDILEAYYSYNADGIFISNGPGNPTL
LKEVAKRIRKAFNEEIPMMGICLGQQLLALADGAEIYKLKYGHRGINKPVKDLESGKAFVTTQNHGYAIVPESLNEFKVW
MVNLDDNSVEGIKHESLPIIATQYHPEASPGPWDSLWVFDEFVKILEGRK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=367, Percent_Identity=44.141689373297, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI21361331, Length=372, Percent_Identity=40.0537634408602, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI169790915, Length=372, Percent_Identity=40.0537634408602, Blast_Score=272, Evalue=3e-73,
Organism=Escherichia coli, GI1786215, Length=383, Percent_Identity=36.0313315926893, Blast_Score=231, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI193204318, Length=366, Percent_Identity=41.2568306010929, Blast_Score=279, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6324878, Length=373, Percent_Identity=43.1635388739946, Blast_Score=303, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6322331, Length=391, Percent_Identity=39.386189258312, Blast_Score=292, Evalue=5e-80,
Organism=Drosophila melanogaster, GI45555749, Length=381, Percent_Identity=39.3700787401575, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24642586, Length=381, Percent_Identity=39.3700787401575, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_PYRFU (Q8U086)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579442.1
- ProteinModelPortal:   Q8U086
- SMR:   Q8U086
- EnsemblBacteria:   EBPYRT00000005649
- GeneID:   1469590
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1713
- NMPDR:   fig|186497.1.peg.1761
- GeneTree:   EBGT00050000022457
- HOGENOM:   HBG286341
- OMA:   EADIPFF
- ProtClustDB:   PRK12564
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_A
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 41715; Mature: 41583

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 263-263 ACT_SITE 346-346 ACT_SITE 348-348

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVHKKGYLVLEDGSVIEGKAFGAETIRYGEVVFTTAMVGYPESLTDPSYKGQILIATNP
CCCCCCCEEEEECCCEEECCCCCCCCEEECCEEEEEEECCCCCCCCCCCCCCEEEEEECC
LMGTYGVSSKSLKEHGLPLHYESDSIKVEGFVISFLMRPNHWSSEMTLDEWLRREGVPGL
CEEECCCCCHHHHHCCCCEEECCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHCCCCCE
YGVDTRALVKKIREEGVMRGAIVTTETDLEEILENIRKISYESTNFVELVSPKNPIVHTP
ECCCHHHHHHHHHHCCCEECEEEECHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECC
KNVKFRVVVLDLGVKFGILRELLKRGIEVVRIPWNWDILEAYYSYNADGIFISNGPGNPT
CCCEEEEEEEECCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCCHH
LLKEVAKRIRKAFNEEIPMMGICLGQQLLALADGAEIYKLKYGHRGINKPVKDLESGKAF
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCCCEE
VTTQNHGYAIVPESLNEFKVWMVNLDDNSVEGIKHESLPIIATQYHPEASPGPWDSLWVF
EEECCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHH
DEFVKILEGRK
HHHHHHHCCCC
>Mature Secondary Structure 
GVHKKGYLVLEDGSVIEGKAFGAETIRYGEVVFTTAMVGYPESLTDPSYKGQILIATNP
CCCCCCEEEEECCCEEECCCCCCCCEEECCEEEEEEECCCCCCCCCCCCCCEEEEEECC
LMGTYGVSSKSLKEHGLPLHYESDSIKVEGFVISFLMRPNHWSSEMTLDEWLRREGVPGL
CEEECCCCCHHHHHCCCCEEECCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHCCCCCE
YGVDTRALVKKIREEGVMRGAIVTTETDLEEILENIRKISYESTNFVELVSPKNPIVHTP
ECCCHHHHHHHHHHCCCEECEEEECHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECC
KNVKFRVVVLDLGVKFGILRELLKRGIEVVRIPWNWDILEAYYSYNADGIFISNGPGNPT
CCCEEEEEEEECCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCCHH
LLKEVAKRIRKAFNEEIPMMGICLGQQLLALADGAEIYKLKYGHRGINKPVKDLESGKAF
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCCCEE
VTTQNHGYAIVPESLNEFKVWMVNLDDNSVEGIKHESLPIIATQYHPEASPGPWDSLWVF
EEECCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHH
DEFVKILEGRK
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA