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The map label for this gene is metG [H]

Identifier: 18978039

GI number: 18978039

Start: 1550606

End: 1550935

Strand: Direct

Name: metG [H]

Synonym: PF1667

Alternate gene names: 18978039

Gene position: 1550606-1550935 (Clockwise)

Preceding gene: 33359570

Following gene: 18978040

Centisome position: 81.26

GC content: 32.73

Gene sequence:

>330_bases
ATGGAGTTATATGACGTTTCAGAATTTTGGAAGTTTGATATGAGAGTTGGAAAGGTAATAAAGGCAGAAAAGTTAAAGAG
AACTAGGAAATTGATAAAGTTGGTTGTTGATTTTGGAAAAGAAACGAGAACAATAGTAACTGGAATAGCAGATCAATATA
ACCCCAAAGATTTAGAAGGGAAGAAATTTATTTTTGTTCTAAACTTAAAACCAAAAGAATTCTCTGGAGTTAAAAGTGAA
GGAATGCTAATAGTGGCAGAAGAAGAAAATGGAAAAGTTTACTTACTTCCTGTTCCAGAAGAAGTTCCGGTAGGAACAAA
AGTTTGGTAG

Upstream 100 bases:

>100_bases
TTCAAAGGCAACGTTACCTACCTCATGGTCGGGAGAGATGTCAATGATGTCAACGAAGCATTGAAAAAGATACTTTTCCA
ATCATATATAAGGTGATTTT

Downstream 100 bases:

>100_bases
AGATCAGCACTTAAGATCCTCATCATCCCTTCAGAGCTGCGCGGGTTCATCATCTTTACCACAAGATCTGAAAGCATTTT
TATATTTTTATGTGCTAAGT

Product: partial methionyl-tRNA synthetase - matches COOH terminus

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 109; Mature: 109

Protein sequence:

>109_residues
MELYDVSEFWKFDMRVGKVIKAEKLKRTRKLIKLVVDFGKETRTIVTGIADQYNPKDLEGKKFIFVLNLKPKEFSGVKSE
GMLIVAEEENGKVYLLPVPEEVPVGTKVW

Sequences:

>Translated_109_residues
MELYDVSEFWKFDMRVGKVIKAEKLKRTRKLIKLVVDFGKETRTIVTGIADQYNPKDLEGKKFIFVLNLKPKEFSGVKSE
GMLIVAEEENGKVYLLPVPEEVPVGTKVW
>Mature_109_residues
MELYDVSEFWKFDMRVGKVIKAEKLKRTRKLIKLVVDFGKETRTIVTGIADQYNPKDLEGKKFIFVLNLKPKEFSGVKSE
GMLIVAEEENGKVYLLPVPEEVPVGTKVW

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789455, Length=109, Percent_Identity=33.0275229357798, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 12548; Mature: 12548

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELYDVSEFWKFDMRVGKVIKAEKLKRTRKLIKLVVDFGKETRTIVTGIADQYNPKDLEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
KKFIFVLNLKPKEFSGVKSEGMLIVAEEENGKVYLLPVPEEVPVGTKVW
CEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MELYDVSEFWKFDMRVGKVIKAEKLKRTRKLIKLVVDFGKETRTIVTGIADQYNPKDLEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
KKFIFVLNLKPKEFSGVKSEGMLIVAEEENGKVYLLPVPEEVPVGTKVW
CEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]