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The map label for this gene is hisF [H]

Identifier: 18978035

GI number: 18978035

Start: 1547441

End: 1548241

Strand: Direct

Name: hisF [H]

Synonym: PF1663

Alternate gene names: 18978035

Gene position: 1547441-1548241 (Clockwise)

Preceding gene: 18978034

Following gene: 18978036

Centisome position: 81.09

GC content: 41.95

Gene sequence:

>801_bases
ATGAAGGAACTATCTCTCTTGAAGAAGCTTTGGAGGTTGCTAAAAGTGTTGGCAAGAAGAATTATAGCAGCTCTTGACAT
AAAAGATGGTAGGGTTGTTAAAGGAATAAAATTCAAGAACATTAGAGATGCTGGAGATCCAATAGAACTAGCTAAAAGGT
ATGAGAGCGAAGGAATTGACGAGATAGTCTTTCTAGATATTACTGCCTCCTACGAAAAAAGGAAAATCCTTCTCGACTTG
GTAGAAAGAGTAGCAGAGGAAATCTACGTTCCTTTTACGGTGGGAGGAGGTATAAGAACAGTTGAAGAGGCAAGAGAAAT
AATTAGACGAGGGGCCGACAAGATATTTATCAATACAGCCGCCGTGGAAAATCCCTCCTTAATTAAAGAGATCGCTGACC
TCATAGGCTCAGCAAACTTAGTTGTTGCTATAGATGCCAAGTGGAACGGAAAATTCTGGGAAGTTTATACACACGGGGGA
AGAAAGCCCAGGGGAATGGACGCTGTTGAGTGGGCCAAAAGAGTAGAAGAACTGGGAGCAGGTGAAATTCTCCTCACGAG
CATGGACACCGATGGAACAAAGCAGGGATTTGACATTCCACTAACAAAAGCAGTTGTTGATGCCGTAAATATTCCCGTTA
TAGCCTCTGGAGGGGCTGGAAAACCAGAACACTTTTTAGAAGTATTTAGGATAGGTTCTGATGCTGCATTAGCGGCATCA
ATTTTCCACTATGGTGAATATACGGTAAGGGAACTGAAGATGTTTCTTGCCAAAAATGGAATTCCAGTTAGACTTGATTA
G

Upstream 100 bases:

>100_bases
GATGAAGAATTTATTTATGCAGGAGGAGTGGCAACACCTACCGATATAATTCAGCTGGCTGAAATAGGGTTTTCTGGCGT
TATTATTGGGAAGGCTTTGT

Downstream 100 bases:

>100_bases
AGGTGAGCATATGGAAATTGAAAAGTTATTGGAGCAAGTAAACTGGGAAAAGAACAATGGGATTGTCCCCGTCATAGTTC
AGGATGAAAAAGGAGAAGTT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKELSLLKKLWRLLKVLARRIIAALDIKDGRVVKGIKFKNIRDAGDPIELAKRYESEGIDEIVFLDITASYEKRKILLDL
VERVAEEIYVPFTVGGGIRTVEEAREIIRRGADKIFINTAAVENPSLIKEIADLIGSANLVVAIDAKWNGKFWEVYTHGG
RKPRGMDAVEWAKRVEELGAGEILLTSMDTDGTKQGFDIPLTKAVVDAVNIPVIASGGAGKPEHFLEVFRIGSDAALAAS
IFHYGEYTVRELKMFLAKNGIPVRLD

Sequences:

>Translated_266_residues
MKELSLLKKLWRLLKVLARRIIAALDIKDGRVVKGIKFKNIRDAGDPIELAKRYESEGIDEIVFLDITASYEKRKILLDL
VERVAEEIYVPFTVGGGIRTVEEAREIIRRGADKIFINTAAVENPSLIKEIADLIGSANLVVAIDAKWNGKFWEVYTHGG
RKPRGMDAVEWAKRVEELGAGEILLTSMDTDGTKQGFDIPLTKAVVDAVNIPVIASGGAGKPEHFLEVFRIGSDAALAAS
IFHYGEYTVRELKMFLAKNGIPVRLD
>Mature_266_residues
MKELSLLKKLWRLLKVLARRIIAALDIKDGRVVKGIKFKNIRDAGDPIELAKRYESEGIDEIVFLDITASYEKRKILLDL
VERVAEEIYVPFTVGGGIRTVEEAREIIRRGADKIFINTAAVENPSLIKEIADLIGSANLVVAIDAKWNGKFWEVYTHGG
RKPRGMDAVEWAKRVEELGAGEILLTSMDTDGTKQGFDIPLTKAVVDAVNIPVIASGGAGKPEHFLEVFRIGSDAALAAS
IFHYGEYTVRELKMFLAKNGIPVRLD

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=44.7470817120623, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI87082028, Length=238, Percent_Identity=25.2100840336134, Blast_Score=75, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=35.5555555555556, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 29503; Mature: 29503

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELSLLKKLWRLLKVLARRIIAALDIKDGRVVKGIKFKNIRDAGDPIELAKRYESEGID
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECEEECCCCCCCCHHHHHHHHHHCCCC
EIVFLDITASYEKRKILLDLVERVAEEIYVPFTVGGGIRTVEEAREIIRRGADKIFINTA
EEEEEEEECCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEC
AVENPSLIKEIADLIGSANLVVAIDAKWNGKFWEVYTHGGRKPRGMDAVEWAKRVEELGA
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCC
GEILLTSMDTDGTKQGFDIPLTKAVVDAVNIPVIASGGAGKPEHFLEVFRIGSDAALAAS
CCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCHHHHHH
IFHYGEYTVRELKMFLAKNGIPVRLD
HHHCCCHHHHHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MKELSLLKKLWRLLKVLARRIIAALDIKDGRVVKGIKFKNIRDAGDPIELAKRYESEGID
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECEEECCCCCCCCHHHHHHHHHHCCCC
EIVFLDITASYEKRKILLDLVERVAEEIYVPFTVGGGIRTVEEAREIIRRGADKIFINTA
EEEEEEEECCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEC
AVENPSLIKEIADLIGSANLVVAIDAKWNGKFWEVYTHGGRKPRGMDAVEWAKRVEELGA
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCC
GEILLTSMDTDGTKQGFDIPLTKAVVDAVNIPVIASGGAGKPEHFLEVFRIGSDAALAAS
CCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCHHHHHH
IFHYGEYTVRELKMFLAKNGIPVRLD
HHHCCCHHHHHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA