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The map label for this gene is eno [H]

Identifier: 18978013

GI number: 18978013

Start: 1531828

End: 1532847

Strand: Reverse

Name: eno [H]

Synonym: PF1641

Alternate gene names: 18978013

Gene position: 1532847-1531828 (Counterclockwise)

Preceding gene: 18978014

Following gene: 18978012

Centisome position: 80.33

GC content: 40.88

Gene sequence:

>1020_bases
ATGAGCATTATTCAAGATATAATTGGGAGAATTGTCGTTTTAAGGGGAGGACAATACTCTGTAGAAGTTGACGTTGTTAC
TGATGAAAGTTTTGGTAGGTTCGCTGCTCCCATTGATGAAAATCCTGCCCTCTATATAGCTGAGGCAAGGAGAGCTGTCA
GTGAAGTTGATGAAATAATTGGCCCAGAGTTGATAGGGTTTGATGCAATTGATCAAGAGCTTATTGATAGCTACCTTTGG
GAAATTGATGGTACTGATAACTTTAGCCATATAGGAGCAAATACAGCACTGGCAGTTTCCGTAGCTGTGGCAAAGGCTGC
CGCGAATTCTAGGAATATCTCCTTGTACTCTTATATAGGAGGTACATTCACAACCGAACTCCCAGTGCCAGTAGTTACAT
TTGGAGCTGATGAAAATTTTGAATATCACGTGATAGTGAGAGACCTCCTGGAAGTCACTGATATCATAGATGCCGTTGTT
CGCCTACTAGATTTTTCCCTCTCCTTAGAAGAGCTATCAAAGGCTTCCGAGCAAGTTGGTTTGGAATTAGGTTTGGAGGT
TAGCCTTGGTATTACAATGAGAAAAGAGCTGGAAACCGAAAAGGTTCTAGAAATTGTTGAGGACTACAATATTGCTTACA
TAAAGCCCATTGGAACTCCAGAGCTATTTCTAGAGCTGATAGCTGGCACCCATGGGGTATTTATAGACGGGGAGTACCTG
TACAGGAAGTATAACATACTCGACAGGAAGTACTATAACGCATTATCAATAAAACCTATAAACCTGGGAACGCTCACTGA
TCTATACAACTTCGTGAATGATGTGAAAGCAGAAAAAATAACCCCAATTTTGTCCGAAAGTAAATATGAGCCTGCAGATG
AAACTCTACCACACCTTGCCCTGGGTTTGAGATGTCCTGCCATGCTGATCCACATGAACTCTGTTGAAAAAATAAATGAA
TTGAATAGAATTGCTGAAGAGTTGGGTGAAAGAGGTAGGATAATAACGTTTGAAGAATAG

Upstream 100 bases:

>100_bases
CATAGACGTGCCTCCGGGAATAACACCGCTTGAAGCAGCTATCTTGGAGTTTGAAAAGGGTGTAATCCCAATAACAGTAA
TCAGACCGAGCTGATGGAAG

Downstream 100 bases:

>100_bases
GGGGTGTTGAGAAATGGCTGATGAGTATCTAGTTCCACTCGACCAGTATTTGGCTGCTGGAGTGCACATAGGAACACAGC
AAAAAACCAAAGACATGAAG

Product: hypothetical protein

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSIIQDIIGRIVVLRGGQYSVEVDVVTDESFGRFAAPIDENPALYIAEARRAVSEVDEIIGPELIGFDAIDQELIDSYLW
EIDGTDNFSHIGANTALAVSVAVAKAAANSRNISLYSYIGGTFTTELPVPVVTFGADENFEYHVIVRDLLEVTDIIDAVV
RLLDFSLSLEELSKASEQVGLELGLEVSLGITMRKELETEKVLEIVEDYNIAYIKPIGTPELFLELIAGTHGVFIDGEYL
YRKYNILDRKYYNALSIKPINLGTLTDLYNFVNDVKAEKITPILSESKYEPADETLPHLALGLRCPAMLIHMNSVEKINE
LNRIAEELGERGRIITFEE

Sequences:

>Translated_339_residues
MSIIQDIIGRIVVLRGGQYSVEVDVVTDESFGRFAAPIDENPALYIAEARRAVSEVDEIIGPELIGFDAIDQELIDSYLW
EIDGTDNFSHIGANTALAVSVAVAKAAANSRNISLYSYIGGTFTTELPVPVVTFGADENFEYHVIVRDLLEVTDIIDAVV
RLLDFSLSLEELSKASEQVGLELGLEVSLGITMRKELETEKVLEIVEDYNIAYIKPIGTPELFLELIAGTHGVFIDGEYL
YRKYNILDRKYYNALSIKPINLGTLTDLYNFVNDVKAEKITPILSESKYEPADETLPHLALGLRCPAMLIHMNSVEKINE
LNRIAEELGERGRIITFEE
>Mature_338_residues
SIIQDIIGRIVVLRGGQYSVEVDVVTDESFGRFAAPIDENPALYIAEARRAVSEVDEIIGPELIGFDAIDQELIDSYLWE
IDGTDNFSHIGANTALAVSVAVAKAAANSRNISLYSYIGGTFTTELPVPVVTFGADENFEYHVIVRDLLEVTDIIDAVVR
LLDFSLSLEELSKASEQVGLELGLEVSLGITMRKELETEKVLEIVEDYNIAYIKPIGTPELFLELIAGTHGVFIDGEYLY
RKYNILDRKYYNALSIKPINLGTLTDLYNFVNDVKAEKITPILSESKYEPADETLPHLALGLRCPAMLIHMNSVEKINEL
NRIAEELGERGRIITFEE

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the archaeal cel

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=147, Percent_Identity=35.3741496598639, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI301897477, Length=147, Percent_Identity=34.0136054421769, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI301897469, Length=147, Percent_Identity=34.0136054421769, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4503571, Length=150, Percent_Identity=34.6666666666667, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536383, Length=147, Percent_Identity=36.0544217687075, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71995829, Length=147, Percent_Identity=36.0544217687075, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI32563855, Length=358, Percent_Identity=24.5810055865922, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323985, Length=135, Percent_Identity=36.2962962962963, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324974, Length=135, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324969, Length=135, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 37723; Mature: 37592

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIQDIIGRIVVLRGGQYSVEVDVVTDESFGRFAAPIDENPALYIAEARRAVSEVDEII
CCHHHHHHHHEEEEECCCEEEEEEEEECCCCCCEECCCCCCCCEEHHHHHHHHHHHHHHH
GPELIGFDAIDQELIDSYLWEIDGTDNFSHIGANTALAVSVAVAKAAANSRNISLYSYIG
CCCCCCCHHHHHHHHHHHHEECCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
GTFTTELPVPVVTFGADENFEYHVIVRDLLEVTDIIDAVVRLLDFSLSLEELSKASEQVG
CCEEECCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
LELGLEVSLGITMRKELETEKVLEIVEDYNIAYIKPIGTPELFLELIAGTHGVFIDGEYL
CEECCEEEECEEEHHHCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEECCHHH
YRKYNILDRKYYNALSIKPINLGTLTDLYNFVNDVKAEKITPILSESKYEPADETLPHLA
HHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHH
LGLRCPAMLIHMNSVEKINELNRIAEELGERGRIITFEE
HHHCCCCCEEECHHHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
SIIQDIIGRIVVLRGGQYSVEVDVVTDESFGRFAAPIDENPALYIAEARRAVSEVDEII
CHHHHHHHHEEEEECCCEEEEEEEEECCCCCCEECCCCCCCCEEHHHHHHHHHHHHHHH
GPELIGFDAIDQELIDSYLWEIDGTDNFSHIGANTALAVSVAVAKAAANSRNISLYSYIG
CCCCCCCHHHHHHHHHHHHEECCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
GTFTTELPVPVVTFGADENFEYHVIVRDLLEVTDIIDAVVRLLDFSLSLEELSKASEQVG
CCEEECCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
LELGLEVSLGITMRKELETEKVLEIVEDYNIAYIKPIGTPELFLELIAGTHGVFIDGEYL
CEECCEEEECEEEHHHCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEECCHHH
YRKYNILDRKYYNALSIKPINLGTLTDLYNFVNDVKAEKITPILSESKYEPADETLPHLA
HHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHH
LGLRCPAMLIHMNSVEKINELNRIAEELGERGRIITFEE
HHHCCCCCEEECHHHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]